11,173 research outputs found
Synthesis of stabilized analogues of fragments of Neisseria meningitidis type A capsular polysaccharide
The resistance to Neisseria meningitidis (N. m.), a gram-negative bacterium causing meningitis, is mediated by the presence of antibodies directed against its capsular polysaccharides (CPS), which are associated with different serogroups. One of the most virulent is serotype A (Men A), whose CPS is constituted of (\uf0ae6)-N-acetylmannosamine \uf061-1-O-Phosphate as a repeating unit. The already existing vaccines against N.m. give a T-cell independent immune response, therefore inefficient in infants. The response can be improved by conjugation of the saccharidic portion to a protein carrier, which invokes T-cell involvement. In addition, anomeric phosphodiester bridges are chemically labile and therefore not appropriate for a vaccine. To overcome these problems, phosphonate 4 and its oligomers 5 and 7 have been synthesised as stable analogues of the repeating unit of Men A CPS. Key steps of the synthetic strategy are:
a) Synthesis of building blocks 3 and 4, the latter bearing a spacer at the reducing end suitable for conjugation to a proper protein carrier;
b) Coupling of 3 with 4 via Mitsunobu reaction providing 5, followed by 6'-O-deacetylation and a second Mitsunobu coupling to give trimer 7.
After removal of the protecting groups, the synthesized compounds have been evaluated through a competitive ELISA assay (IC50 values) by use of Men A antiserum. Moreover, preliminary approaches towards the carba-analogue 8 of N-acetylmannosamine 1-O-phosphate, a key building block for the synthesis of a new type of Men A analogues, will be also discussed
Spectroscopic classification of X-ray sources in the Galactic Bulge Survey
We present the classification of 26 optical counterparts to X-ray sources
discovered in the Galactic Bulge Survey. We use (time-resolved) photometric and
spectroscopic observations to classify the X-ray sources based on their
multi-wavelength properties. We find a variety of source classes, spanning
different phases of stellar/binary evolution. We classify CX21 as a quiescent
cataclysmic variable (CV) below the period gap, and CX118 as a high accretion
rate (nova-like) CV. CXB12 displays excess UV emission, and could contain a
compact object with a giant star companion, making it a candidate symbiotic
binary or quiescent low mass X-ray binary (although other scenarios cannot be
ruled out). CXB34 is a magnetic CV (polar) that shows photometric evidence for
a change in accretion state. The magnetic classification is based on the
detection of X-ray pulsations with a period of 81 2 min. CXB42 is
identified as a young stellar object, namely a weak-lined T Tauri star
exhibiting (to date unexplained) UX Ori-like photometric variability. The
optical spectrum of CXB43 contains two (resolved) unidentified double-peaked
emission lines. No known scenario, such as an AGN or symbiotic binary, can
easily explain its characteristics. We additionally classify 20 objects as
likely active stars based on optical spectroscopy, their X-ray to optical flux
ratios and photometric variability. In 4 cases we identify the sources as
binary stars.Comment: Accepted for publication in MNRA
Long-term exercise modulates hippocampal gene expression in sencescent females mice
Altres ajuts: FI-DGR 2011 de la Generalitat de CatalunyaThe senescence-accelerated SAMP8 mouse is considered a useful non-transgenic model for studying aspects of progressive cognitive decline and Alzheimer's disease (AD). Using SAMR1 mice as controls, here we explored the effects of 6 months of voluntary wheel running in 10-month-old female SAMP8 mice. Exercise in SAMP8 mice improved phenotypic features associated with premature aging (i.e., skin color and body tremor) and enhanced vascularization and BDNF gene expression in the hippocampus compared with controls. With the aim of identifying genes involved in brain aging responsive to long-term exercise, we performed whole genome microarray studies in hippocampus from sedentary SAMP8 (P8sed), SAMR1 (R1sed), and exercised SAMP8 (P8run) mice. The genes differentially expressed in P8sed versus R1sed were considered as putative aging markers (i) and those differentially expressed in P8run versus P8sed were considered as genes modulated by exercise (ii). Genes differentially expressed in both comparisons (i and ii) were considered as putative aging genes responsive to physical exercise. We identified 34 genes which met both criteria. Gene ontology analysis revealed that they are mainly involved in functions related to extracellular matrix maintenance. Selected genes were validated by real-time quantitative PCR assays, i.e., collagen type 1 alpha 1 (col1a1), collagen type 1 alpha 2 (col1a2), fibromodulin (fmod), prostaglandin D(2) synthase (ptgds), and aldehyde dehydrogenase (Aldh1a2). As a whole, our study suggests that exercise training during adulthood may prevent or delay gene expression alterations and processes associated with hippocampal aging in at-risk subjects
Relationship between cognition and age at onset of first-episode psychosis: comparative study between adolescents, young adults, and adults
Psychotic disorders typically manifest from late adolescence to early adulthood, and an earlier onset might be associated with greater symptom severity and a worse long-term prognosis. This study aimed to compare the cognitive characteristics of patients with first-episode psychosis (FEP) by their age at onset. We included 298 patients diagnosed with FEP and classified them as having an early onset (EOS), youth onset (YOS), or adult onset (AOS) based on age limits of = 25 years (N = 116), respectively. Socio-demographic and clinical variables included age at baseline, gender, socio-economic status, antipsychotic medication, DSM-IV diagnoses assessed by clinical semi-structured interview, psychotic symptom severity, and age at onset. Neuropsychological assessment included six cognitive domains: premorbid intelligence, working memory, processing speed, verbal memory, sustained attention, and executive functioning. The EOS group had lower scores than the YOS or AOS groups in global cognition, executive functioning, and sustained attention. Although the scores in the YOS group were intermediate to those in the EOS and AOS groups for most cognitive factors, no statistically significant differences were detected between the YOS and AOS groups. Age at onset results in specific patterns of cognitive interference. Of note, impairment appears to be greater with EOS samples than with either YOS or AOS samples. A longitudinal study with a larger sample size is needed to confirm our findings
Biological treatments in allergy: Prescribing patterns and management of hypersensitivity reactions
Clinical Communication
Pentagonal nanowires: a first-principles study of atomic and electronic structure
We performed an extensive first-principles study of nanowires in various
pentagonal structures by using pseudopotential plane wave method within the
density functional theory. Our results show that nanowires of different types
of elements, such as alkali, simple, transition and noble metals and inert gas
atoms, have a stable structure made from staggered pentagons with a linear
chain perpendicular to the planes of the pentagons and passing through their
centers. This structure exhibits bond angles close to those in the icosahedral
structure. However, silicon is found to be energetically more favorable in the
eclipsed pentagonal structure. These quasi one dimensional pentagonal nanowires
have higher cohesive energies than many other one dimensional structures and
hence may be realized experimentally. The effect of magnetic state are examined
by spin-polarized calculations. The origin of the stability are discussed by
examining optimized structural parameters, charge density and electronic band
structure, and by using analysis based on the empirical Lennard-Jones type
interaction. Electronic band structure of pentagonal wires of different
elements are discussed and their effects on quantum ballistic conductance are
mentioned. It is found that the pentagonal wire of silicon exhibits metallic
band structure.Comment: 4 figures, accepted for publication in Phys. Rev.
First bounds on the high-energy emission from isolated Wolf-Rayet binary systems
High-energy gamma-ray emission is theoretically expected to arise in tight
binary star systems (with high mass loss and high velocity winds), although the
evidence of this relationship has proven to be elusive so far. Here we present
the first bounds on this putative emission from isolated Wolf-Rayet (WR) star
binaries, WR 147 and WR 146, obtained from observations with the MAGIC
telescope.Comment: (Authors are the MAGIC Collaboration.) Manuscript in press at The
Astrophysical Journal Letter
Determining social factors related to pesticide poisoning among rice farmers in Colombia
Objetivo Caracterizar la forma en que los agricultores se exponen a los plaguicidas y se presenta la intoxicación. Materiales y Métodos Estudio multi-métodos y multinivel (individuo y comunidad), que incluyó métodos etnográficos, encuesta y medición de plaguicidas en agua y muestras humanas. Resultados Se describió el proceso productivo y se conocieron los principales factores de riesgo. Los plaguicidas son considerados el mayor peligro en el trabajo y en sus viviendas. Los trabajadores tienen precarias condiciones laborales y no están protegidos por el sistema de riesgos laborales. En las muestras de agua se encontraron azinfos-metil, endosulfán, b-BHC, bromofos-metil, bromofos-etil y 2,4-DDT. En la encuesta participaron 381 trabajadores de los cuales 12,9, 68 y 5,5 % tuvieron intoxicaciones leves, moderadas y severas, respectivamente. Los casos severos tenían menores niveles de escolaridad, menor nivel de afiliación al régimen contributivo del sistema de seguridad social y un mayor número tenía enfermedad cardiovascular, diabetes, herpes u otras infecciones virales. Conclusión Existen precarias condiciones laborales que favorecen la exposición a plaguicidas caracterizadas por la exclusión del sistema de riesgos laborales, pobreza y baja educación. Es urgente la inclusión de estos trabajadores al sistema de riesgos laborales y la mejora de las condiciones de vida. De esa manera se podrán disminuir las prácticas inseguras en el manejo de plaguicidas.Objective Large quantities of pesticides are used in rice crops. The aim of this study is to characterize how farmers are exposed to pesticides and subsequent poisoning. Materials and Methods A multilevel (individual and community) multi-method study, which included ethnographic and survey methods, as well as measurement of pesticides in water and human samples, was performed. Results The production process is described and the main risk factors are presented. Pesticides are considered the greatest danger at work and at their homes. Workers have poor working conditions and are not protected by the system of occupational risks. Azinphos-methyl, endosulfan, beta-BHC, bromophos-methyl, bromophos-ethyl and 2,4- DDT were found in water samples. The survey included 381 workers with mild (12.86 %), moderate (67.98 %) and severe (5.51 %) poisonings respectively. Severe cases presented lower levels of education, lower levels of health care access to the contributory regimen of the Colombian social security system and higher incidence of cardiovascular disease, diabetes, herpes or other viral infections. Conclusion There are precarious working conditions that favor exposure to pesticides correlated to the exclusion of farmers from the occupational risk system, to poverty and to poor education. It is urgent to include these workers to the system of occupational risk system and to improve their living conditions, thus reducing unsafe practices when handling pesticides
MAGIC observations of very high energy gamma-rays from HESS J1813-178
Recently, the HESS collaboration has reported the detection of gamma-ray
emission above a few hundred GeV from eight new sources located close to the
Galactic Plane. The source HESS J1813-178 has sparked particular interest, as
subsequent radio observations imply an association with SNR G12.82-0.02.
Triggered by the detection in VHE gamma-rays, a positionally coincident source
has also been found in INTEGRAL and ASCA data. In this Letter we present MAGIC
observations of HESS J1813-178, resulting in the detection of a differential
gamma-ray flux consistent with a hard-slope power law, described as dN/(dA dt
dE) = (3.3+/-0.5)*10^{-12} (E/TeV)^{-2.1+/-0.2} cm^(-2)s^(-1)TeV^(-1). We
briefly discuss the observational technique used, the procedure implemented for
the data analysis, and put this detection in the perspective of multifrequency
observations.Comment: Accepted by ApJ Letter
Observation of Pulsed Gamma-rays Above 25 GeV from the Crab Pulsar with MAGIC
One fundamental question about pulsars concerns the mechanism of their pulsed
electromagnetic emission. Measuring the high-end region of a pulsar's spectrum
would shed light on this question. By developing a new electronic trigger, we
lowered the threshold of the Major Atmospheric gamma-ray Imaging Cherenkov
(MAGIC) telescope to 25 GeV. In this configuration, we detected pulsed
gamma-rays from the Crab pulsar that were greater than 25 GeV, revealing a
relatively high cutoff energy in the phase-averaged spectrum. This indicates
that the emission occurs far out in the magnetosphere, hence excluding the
polar-cap scenario as a possible explanation of our measurement. The high
cutoff energy also challenges the slot-gap scenario.Comment: Slight modification of the analysis: Fitting a more general function
to the combined data set of COMPTEL, EGRET and MAGIC. Final result and
conclusion is unchange
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