Photoaffinity Labeling the Agonist Binding Sites of Torpedo and α4β2 Nicotinic Acetylcholine Receptors and Acetylcholine Binding Proteins (AChBPs) with [3H]Cytisine

Fitxes dels barri

The spatial distribution and annual cycle of upper ocean thermohaline structure

Author Posting. © American Geophysical Union, 2012. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 117 (2012): C02027, doi:10.1029/2011JC007033.Observations of the spatial distribution and persistence of thermohaline structure are presented, and show how advection and diffusion affect a passive tracer. More than two years of underwater glider observations in the central subtropical North Pacific showed thermohaline variability over horizontal scales from 5 to 1300 km. Thermohaline fluctuations along isopycnals (spice fluctuations) were elevated in layers throughout the water column with the largest fluctuations near the surface and subtropical frontal regions. Fluctuations were uncorrelated between the layers but stirred by the same velocity field. Spice variance had local extrema in the vertical because of differences in source water properties and the influence of neighboring water masses. Spice variance spanned about three orders of magnitude along deeper isopycnals with larger variance where different water masses met and where velocity and vorticity variance were elevated. Horizontal wave number spectra of spice had slopes of −2 everywhere in the upper 1000 m. Submesoscale spice fluctuations had slopes in physical space near the ratio of the Coriolis parameter to the buoyancy frequency (f/N), consistent with predictions of quasi-geostrophic theory. In the mixed layer, thermohaline structure had a significant annual cycle with smaller interannual differences. Thermohaline fluctuations left behind during restratification and isolated from the mixed layer decayed with time because of diffusion along isopycnals. Horizontal diffusivity estimates in the remnant mixed layer were 0.4 m2 s−1 at 15–28 km wavelengths and 0.9 m2 s−1 at 35–45 km wavelengths.We gratefully acknowledge the National Science Foundation for funding this work under grant number OCE0452574.2012-08-1

Characterizing Ligand-Gated Ion Channel Receptors with Genetically Encoded Ca++ Sensors

We present a cell based system and experimental approach to characterize agonist and antagonist selectivity for ligand-gated ion channels (LGIC) by developing sensor cells stably expressing a Ca2+ permeable LGIC and a genetically encoded Förster (or fluorescence) resonance energy transfer (FRET)-based calcium sensor. In particular, we describe separate lines with human α7 and human α4β2 nicotinic acetylcholine receptors, mouse 5-HT3A serotonin receptors and a chimera of human α7/mouse 5-HT3A receptors. Complete concentration-response curves for agonists and Schild plots of antagonists were generated from these sensors and the results validate known pharmacology of the receptors tested. Concentration-response relations can be generated from either the initial rate or maximal amplitudes of FRET-signal. Although assaying at a medium throughput level, this pharmacological fluorescence detection technique employs a clonal line for stability and has versatility for screening laboratory generated congeners as agonists or antagonists on multiple subtypes of ligand-gated ion channels. The clonal sensor lines are also compatible with in vivo usage to measure indirectly receptor activation by endogenous neurotransmitters

The state of the Martian climate

60°N was +2.0°C, relative to the 1981–2010 average value (Fig. 5.1). This marks a new high for the record. The average annual surface air temperature (SAT) anomaly for 2016 for land stations north of starting in 1900, and is a significant increase over the previous highest value of +1.2°C, which was observed in 2007, 2011, and 2015. Average global annual temperatures also showed record values in 2015 and 2016. Currently, the Arctic is warming at more than twice the rate of lower latitudes

A dyadic approach to understanding the impact of breast cancer on relationships between partners during early survivorship

© 2016 The Author(s). Background: The shared impact of breast cancer for women and their male partners is emerging as an important consideration during the experience of a breast cancer diagnosis, particularly during survivorship. This study aimed to explore the experiences of women and their partners during early survivorship and contributes a range of insights into the lives of those intimately affected by breast cancer. Methods: In-depth interviews were completed with Australian women survivors of breast cancer (n = 8) and their partners (n = 8), between six months and five years following cessation of treatment. Questions included a focus on the women and their partners' daily experiences during early survivorship, including the management of ongoing symptoms, engagement in leisure and social interests, returning to work, communicating with each other, maintenance of the current relationship and other important roles and responsibilities. Thematic analysis was employed to determine key themes arising from the dyadic accounts of women and their partners' experiences during early breast cancer survivorship. Results: Women and their partners experienced many changes to their previous roles, responsibilities and relationships during early breast cancer survivorship. Couples also reported a range of communication, intimacy and sexuality concerns which greatly impacted their interactions with each other, adding further demands on the relationship. Three significant themes were determined: (1) a disconnection within the relationship - this was expressed as the woman survivor of breast cancer needing to prioritise her own needs, sometimes at the expense of her partner and the relationship; (2) reformulating the relationship - this reflects the strategies used by couples to negotiate changes within the relationship; and (3) support is needed to negotiate the future of the relationship - couples emphasised the need for additional support and resources to assist them in maintaining their relationship during early survivorship. Conclusion: It can be concluded that the early survivorship period represents a crucial time for both women and their partners and there are currently limited options available to meet their shared needs and preferences for support. Findings indicate that a suitable model of care underpinned by a biopsychosocial framework, access to comprehensive assessment, timely support and the provision of targeted resources are urgently needed to assist women and their partners during this critical time