A Tunable, Three-Dimensional \u3ci\u3eIn Vitro\u3c/i\u3e Culture Model of Growth Plate Cartilage Using Alginate Hydrogel Scaffolds

Defining the final size and geometry of engineered tissues through precise control of the scalar and vector components of tissue growth is a necessary benchmark for regenerative medicine, but it has proved to be a significant challenge for tissue engineers. The growth plate cartilage that promotes elongation of the long bones is a good model system for studying morphogenetic mechanisms because cartilage is composed of a single cell type, the chondrocyte; chondrocytes are readily maintained in culture; and growth trajectory is predominately in a single vector. In this cartilage, growth is generated via a differentiation program that is spatially and temporally regulated by an interconnected network composed of long- and short-range signaling mechanisms that together result in the formation of functionally distinct cellular zones. To facilitate investigation of the mechanisms underlying anisotropic growth, we developed an in vitro model of the growth plate cartilage by using neonatal mouse growth plate chondrocytes encapsulated in alginate hydrogel beads. In bead cultures, encapsulated chondrocytes showed high viability, cartilage matrix deposition, low levels of chondrocyte hypertrophy, and a progressive increase in cell proliferation over 7 days in culture. Exogenous factors were used to test functionality of the parathyroid-related protein–Indian hedgehog (PTHrP-IHH) signaling interaction, which is a crucial feedback loop for regulation of growth. Consistent with in vivo observations, exogenous PTHrP stimulated cell proliferation and inhibited hypertrophy, whereas IHH signaling stimulated chondrocyte hypertrophy. Importantly, the treatment of alginate bead cultures with IHH or thyroxine resulted in formation of a discrete domain of hypertrophic cells that mimics tissue architecture of native growth plate cartilage. Together, these studies are the first demonstration of a tunable in vitro system to model the signaling network interactions that are required to induce zonal architecture in growth plate chondrocytes, which could also potentially be used to grow cartilage cultures of specific geometries to meet personalized patient needs

Trans-Gulf of Mexico loop migration of tree swallows revealed by solar geolocation

One of the greatest feats of avian migration is the non-stop crossing of extensive areas of inhospitable habitat such as deserts and seas. Differences in spring and autumn migration routes have been reported in species that cross such barriers, and are thought to have evolved in response to seasonal variation in prevailing wind direction. We tested the hypothesis that migration routes vary seasonally with respect to the Gulf of Mexico in the tree swallow Tachycineta bicolor using solar geolocators attached and retrieved at 4 breeding sites in central North America. We found that 100 % of birds (n = 10) made a trans-Gulf flight of \u3e850 km from Louisiana south to their wintering grounds in the Yucatan Peninsula in 12–36 hours, achieving minimum ground speeds as high as 32 m/s. Although most days during autumn migration were characterized by unfavorable headwinds blowing to the northwest, migration over the Gulf mostly occurred on days with strong winds blowing to the south. In contrast, in 8 of 9 (88 %) birds on spring migration returned from the wintering grounds towards Louisiana following a clockwise loop pat tern flying over land to the west around the Gulf. During this spring period there were few days with prevailing winds from the south to assist northward migration. Results suggest that, despite being up to three times further (ca. 2,700 km), a coastal cir- cum-Gulf spring migration represents the less risky route when wind conditions are not favorable. These findings also help to re solve a long-standing dispute in the literature concerning migration patterns between the US Gulf coast and Mexico, and provide insight into the factors shaping migration strategies of small songbirds migrating across large bodies of water

Metallicities for 13 nearby open clusters from high-resolution spectroscopy of dwarf and giant stars. Stellar metallicity, stellar mass, and giant planets

We present a study of accurate stellar parameters and iron abundances for 39 giants and 16 dwarfs in the 13 open clusters IC2714, IC4651, IC4756, NGC2360, NGC2423, NGC2447 (M93), NGC2539, NGC2682 (M67), NGC3114, NGC3680, NGC4349, NGC5822, NGC6633. The analysis was done using a set of high-resolution and high-S/N spectra obtained with the UVES spectrograph (VLT). These clusters are currently being searched for planets using precise radial velocities. For all the clusters, the derived average metallicities are close to solar. Interestingly, the values derived seem to depend on the line-list used. This dependence and its implications for the study of chemical abundances in giants stars are discussed. We show that a careful choice of the lines may be crucial for the derivation of metallicities for giant stars on the same metallicity scale as those derived for dwarfs. Finally, we discuss the implications of the derived abundances for the metallicity- and mass-giant planet correlation. We conclude that a good knowledge of the two parameters is necessary to correctly disentangle their influence on the formation of giant planets.Comment: accepted for publication in A&

A Hubble Space Telescope transit light curve for GJ436b

We present time series photometry for six partial transits of GJ436b obtained with the Fine Guidance Sensor instrument on the Hubble Space Telescope (HST). Our analysis of these data yields independent estimates of the host star's radius R_star = 0.505 +0.029/-0.020 R_sun, and the planet's orbital period P = 2.643882 +0.000060/-0.000058 d, orbital inclination i = 85.80 +0.21/-0.25 deg, mean central transit time T_c = 2454455.279241 +0.00026/-0.00025 HJD, and radius R_p = 4.90 +0.45/-0.33 R_earth. The radius we determine for the planet is larger than the previous findings from analyses of an infrared light curve obtained with the Spitzer Space Telescope. Although this discrepancy has a 92% formal significance (1.7 sigma), it might be indicative of systematic errors that still influence the analyses of even the highest-precision transit light curves. Comparisons of all the measured radii to theoretical models suggest that GJ436b has a H/He envelope of ~10% by mass. We also find that the transit times for GJ436b are constant to within 10 s over the 11 planetary orbits that the HST data span. However, the ensemble of published values exhibits a long-term drift and our mean transit time is 128 s later than that expected from the Spitzer ephemeris. The sparseness of the currently available data hinders distinguishing between an error in the orbital period or perturbations arising from an additional object in the system as the cause of the apparent trend. Assuming the drift is due to an error in the orbital period we obtain an improved estimate for it of P = 2.643904 +/- 0.000005 d. This value and our measured transit times will serve as important benchmarks in future studies of the GJ436 system. (abridged)Comment: Accepted for publication in A&

Constructing and evaluating a continent‐wide migratory songbird network across the annual cycle

Determining how migratory animals are spatially connected between breeding and non‐breeding periods is essential for predicting the effects of environmental change and for developing optimal conservation strategies. Yet, despite recent advances in tracking technology, we lack comprehensive information on the spatial structure of migratory networks across a species’ range, particularly for small‐bodied, long‐distance migratory animals. We constructed a migratory network for a songbird and used network‐based metrics to characterize the spatial structure and prioritize regions for conservation. The network was constructed using year‐round movements derived from 133 archival light‐level geolocators attached to Tree Swallows (Tachycineta bicolor) originating from 12 breeding sites across their North American breeding range. From these breeding sites, we identified 10 autumn stopover nodes (regions) in North America, 13 non‐breeding nodes located around the Gulf of Mexico, Mexico, Florida, and the Caribbean, and 136 unique edges (migratory routes) connecting nodes. We found strong migratory connectivity between breeding and autumn stopover sites and moderate migratory connectivity between the breeding and non‐breeding sites. We identified three distinct “communities” of nodes that corresponded to western, central, and eastern North American flyways. Several regions were important for maintaining network connectivity, with South Florida and Louisiana as the top ranked non‐breeding nodes and the Midwest as the top ranked stopover node. We show that migratory songbird networks can have both a high degree of mixing between seasons yet still show regionally distinct migratory flyways. Such information will be crucial for accurately predicting factors that limit and regulate migratory songbirds throughout the annual cycle. Our study highlights how network‐based metrics can be valuable for identifying overall network structure and prioritizing specific regions within a network for conserving a wide variety of migratory animals

Two-Loop Analysis of Non-abelian Chern-Simons Theory

Perturbative renormalization of a non-Abelian Chern-Simons gauge theory is examined. It is demonstrated by explicit calculation that, in the pure Chern-Simons theory, the beta-function for the coefficient of the Chern-Simons term vanishes to three loop order. Both dimensional regularization and regularization by introducing a conventional Yang-Mills component in the action are used. It is shown that dimensional regularization is not gauge invariant at two loops. A variant of this procedure, similar to regularization by dimensional reduction used in supersymmetric field theories is shown to obey the Slavnov-Taylor identity to two loops and gives no renormalization of the Chern-Simons term. Regularization with Yang-Mills term yields a finite integer-valued renormalization of the coefficient of the Chern-Simons term at one loop, and we conjecture no renormalization at higher order. We also examine the renormalization of Chern-Simons theory coupled to matter. We show that in the non-abelian case the Chern-Simons gauge field as well as the matter fields require infinite renormalization at two loops and therefore obtain nontrivial anomalous dimensions. We show that the beta function for the gauge coupling constant is zero to two-loop order, consistent with the topological quantization condition for this constant.Comment: 48 pages, UU/HEP/91/12; file format changed to standard Latex to solve the problem with printin

Treating symptomatic uterine fibroids with myomectomy: current practice and views of UK consultants

Background: The demand for uterus-sparing treatments is increasing as more women postpone childbirth to their 30–40s, when fibroids are more symptomatic. With an increasing choice of treatment options and changing care-provider profiles, now is an opportune time to survey current practices and opinions. Using a 25-stem questionnaire, a web-based survey was used to capture the practices and opinions of UK consultant gynecologists on the treatment of symptomatic fibroids, including the types of procedure most frequently used, methods used to reduce blood loss, and awareness and acceptability of treatment options, and to assess the impact of gender and experience of the treating gynecologist. Results: The response rate was 22%. Laparascopic myomectomy is used least frequently, with 80% of the respondents using GnRHa preoperatively to minimize blood loss and correct anemia, while vasopressin is most frequently used to reduce intraoperative blood loss. Female consultants operate significantly less frequently than males. Those with more than 10 years consultant experience are more likely to perform an open myomectomy compared to those with less than 10 years experience. Conclusions: Compared to a similar survey performed 10 years ago, surgical methods remain to be the most common treatments, but use of less invasive treatments such as UAE has increased. Consultants’ attitudes appear to be responding to the patient demand for less radical treatments. However, it is yet to be seen if the changing consultant demographics will keep up with this demand. The low response rate warrants cautious interpretation of the results, but they provide an interesting snapshot of current views and practices

A chemical survey of exoplanets with ARIEL

Thousands of exoplanets have now been discovered with a huge range of masses, sizes and orbits: from rocky Earth-like planets to large gas giants grazing the surface of their host star. However, the essential nature of these exoplanets remains largely mysterious: there is no known, discernible pattern linking the presence, size, or orbital parameters of a planet to the nature of its parent star. We have little idea whether the chemistry of a planet is linked to its formation environment, or whether the type of host star drives the physics and chemistry of the planet’s birth, and evolution. ARIEL was conceived to observe a large number (~1000) of transiting planets for statistical understanding, including gas giants, Neptunes, super-Earths and Earth-size planets around a range of host star types using transit spectroscopy in the 1.25–7.8 μm spectral range and multiple narrow-band photometry in the optical. ARIEL will focus on warm and hot planets to take advantage of their well-mixed atmospheres which should show minimal condensation and sequestration of high-Z materials compared to their colder Solar System siblings. Said warm and hot atmospheres are expected to be more representative of the planetary bulk composition. Observations of these warm/hot exoplanets, and in particular of their elemental composition (especially C, O, N, S, Si), will allow the understanding of the early stages of planetary and atmospheric formation during the nebular phase and the following few million years. ARIEL will thus provide a representative picture of the chemical nature of the exoplanets and relate this directly to the type and chemical environment of the host star. ARIEL is designed as a dedicated survey mission for combined-light spectroscopy, capable of observing a large and well-defined planet sample within its 4-year mission lifetime. Transit, eclipse and phase-curve spectroscopy methods, whereby the signal from the star and planet are differentiated using knowledge of the planetary ephemerides, allow us to measure atmospheric signals from the planet at levels of 10–100 part per million (ppm) relative to the star and, given the bright nature of targets, also allows more sophisticated techniques, such as eclipse mapping, to give a deeper insight into the nature of the atmosphere. These types of observations require a stable payload and satellite platform with broad, instantaneous wavelength coverage to detect many molecular species, probe the thermal structure, identify clouds and monitor the stellar activity. The wavelength range proposed covers all the expected major atmospheric gases from e.g. H2O, CO2, CH4 NH3, HCN, H2S through to the more exotic metallic compounds, such as TiO, VO, and condensed species. Simulations of ARIEL performance in conducting exoplanet surveys have been performed – using conservative estimates of mission performance and a full model of all significant noise sources in the measurement – using a list of potential ARIEL targets that incorporates the latest available exoplanet statistics. The conclusion at the end of the Phase A study, is that ARIEL – in line with the stated mission objectives – will be able to observe about 1000 exoplanets depending on the details of the adopted survey strategy, thus confirming the feasibility of the main science objectives.Peer reviewedFinal Published versio