Diet uniformity at an early farming community in northwest Anatolia (Turkey) : carbon and nitrogen isotope studies of bone collagen at Aktopraklik

Aktopraklık is a settlement site composed of three areas (A–C) in the Marmara region of northwest Anatolia, with phases of occupation that date to the Late Neolithic and Early Chalcolithic periods, mid-seventh to mid-sixth millennium bc (ca. 6400–5600 cal. bc). Here, we present 54 human and fauna bone collagen stable isotope results from the site, alongside five modern fish bone collagen isotope results, to examine the nature of human diet. The stable isotope analysis shows that human diet comprised the consumption of select C3 terrestrial resources, with a preference for domestic animal proteins over plant proteins. The evidence to date suggests that animal husbandry was at the forefront of Late Neolithic and Early Chalcolithic subsistence practices. No isotopic difference in humans is observed between biological sex or between areas B and C at the settlement

Genetic origins of the Minoans and Mycenaeans

The origins of the Bronze Age Minoan and Mycenaean cultures have puzzled archaeologists for more than a century. We assembled genome-wide data from nineteen ancient individuals, including Minoans from Crete, Mycenaeans from mainland Greece, and their eastern neighbours from southwestern Anatolia. We show that Minoans and Mycenaeans were genetically similar, having at least three quarters of their ancestry from the first Neolithic farmers of western Anatolia and the Aegean1,2, and most of the remainder from ancient populations like those of the Caucasus3 and Iran4,5. However, the Mycenaeans differed from Minoans in deriving additional ancestry from an ultimate source related to the hunter-gatherers of eastern Europe and Siberia6–8, introduced via a proximal source related to either the inhabitants of either the Eurasian steppe1,6,9 or Armenia4,9. Modern Greeks resemble the Mycenaeans, but with some additional dilution of the early Neolithic ancestry. Our results support the idea of continuity but not isolation in the history of populations of the Aegean, before and after the time of its earliest civilizations

Genomic insights into the origin of farming in the ancient Near East

We report genome-wide ancient DNA from 44 ancient Near Easterners ranging in time between ~12,000 and 1,400 BC, from Natufian hunter–gatherers to Bronze Age farmers. We show that the earliest populations of the Near East derived around half their ancestry from a ‘Basal Eurasian’ lineage that had little if any Neanderthal admixture and that separated from other non-African lineages before their separation from each other. The first farmers of the southern Levant (Israel and Jordan) and Zagros Mountains (Iran) were strongly genetically differentiated, and each descended from local hunter–gatherers. By the time of the Bronze Age, these two populations and Anatolian-related farmers had mixed with each other and with the hunter–gatherers of Europe to greatly reduce genetic differentiation. The impact of the Near Eastern farmers extended beyond the Near East: farmers related to those of Anatolia spread westward into Europe; farmers related to those of the Levant spread southward into East Africa; farmers related to those of Iran spread northward into the Eurasian steppe; and people related to both the early farmers of Iran and to the pastoralists of the Eurasian steppe spread eastward into South Asia

Genome-wide patterns of selection in 230 ancient Eurasians

Ancient DNA makes it possible to directly witness natural selection by analyzing samples from populations before, during and after adaptation events. Here we report the first scan for selection using ancient DNA, capitalizing on the largest genome-wide dataset yet assembled: 230 West Eurasians dating to between 6500 and 1000 BCE, including 163 with newly reported data. The new samples include the first genome-wide data from the Anatolian Neolithic culture whose genetic material we extracted from the DNA-rich petrous bone and who we show were members of the population that was the source of Europe’s first farmers. We also report a complete transect of the steppe region in Samara between 5500 and 1200 BCE that allows us to recognize admixture from at least two external sources into steppe populations during this period. We detect selection at loci associated with diet, pigmentation and immunity, and two independent episodes of selection on height

The genetic history of the Southern Arc: a bridge between West Asia and Europe

By sequencing 727 ancient individuals from the Southern Arc (Anatolia and its neighbors in Southeastern Europe and West Asia) over 10,000 years, we contextualize its Chalcolithic period and Bronze Age (about 5000 to 1000 BCE), when extensive gene flow entangled it with the Eurasian steppe. Two streams of migration transmitted Caucasus and Anatolian/Levantine ancestry northward, and the Yamnaya pastoralists, formed on the steppe, then spread southward into the Balkans and across the Caucasus into Armenia, where they left numerous patrilineal descendants. Anatolia was transformed by intra–West Asian gene flow, with negligible impact of the later Yamnaya migrations. This contrasts with all other regions where Indo-European languages were spoken, suggesting that the homeland of the Indo-Anatolian language family was in West Asia, with only secondary dispersals of non-Anatolian Indo-Europeans from the steppe

Stable isotope analysis of Neolithic and Chalcolithic populations from Aktopraklik, northern Anatolia

This paper presents the results of stable isotope (carbon and nitrogen) analysis of human and faunal remains from the site of Aktopraklık, one of the earliest farming sites in the Eastern Marmara region of Northwest Anatolia. Excavations at this site have shown that occupation occurs from the middle of the 7th millennium BC through to the middle of the 6th millennium BC. The earliest Neolithic activity at this location occurs at the settlement site of Aktopraklık C. Since 2004 a number of Neolithic and Early Chalcolithic burials have been recovered from the settlement areas and an Early Chalcolithic cemetery (Aktopraklık B and A respectively). To date a total of 60 individuals have been recovered from Aktopraklık, 23 of which (20 adults [10 males, 8 females and 2 indet adults] and 3 children below ca. 12 years of age) form the basis of the current isotope study. In addition, 14 faunal samples from cattle, pig, sheep/goat and fallow deer are included in the analysis in order to facilitate a consideration of trophic level shifts and to interpret the 13C data. The data represents the first isotopic study of a farming community from this region of Anatolia. This region is important to our understanding of the north-westwards transmission of farming into Europe from the Near East, and as such Aktopraklık represents a key site for studying the diet of farmers at the transition to agriculture. The close clustering of isotope values overall indicates homogeneity in subsistence practices for this farming population. Interestingly, the isotope values indicate a general focus on C3 terrestrial resources at Aktopraklık, despite the close proximity of both freshwater and marine environments where alternative resources could have been procured

The genetic history of the Southern Arc: A bridge between West Asia and Europe

Materials and Methods The materials and methods described here are for the combined study of the population history of the Southern Arc and pertain to the present study (which describe the entire dataset and analytically focuses on the Chalcolithic and Bronze Age periods), and two studies on Neolithic populations and the more recent history of the Southern Arc which employ the same analysis dataset and method

Ancient DNA from Mesopotamia suggests distinct Pre-Pottery and Pottery Neolithic migrations into Anatolia

We present the first ancient DNA data from the Pre-Pottery Neolithic of Mesopotamia (Southeastern Turkey and Northern Iraq), Cyprus, and the Northwestern Zagros, along with the first data from Neolithic Armenia. We show that these and neighboring populations were formed through admixture of pre-Neolithic sources related to Anatolian, Caucasus, and Levantine hunter-gatherers, forming a Neolithic continuum of ancestry mirroring the geography of West Asia. By analyzing Pre-Pottery and Pottery Neolithic populations of Anatolia, we show that the former were derived from admixture between Mesopotamian-related and local Epipaleolithic-related sources, but the latter experienced additional Levantine-related gene flow, thus documenting at least two pulses of migration from the Fertile Crescent heartland to the early farmers of Anatolia

The genetic history of the Southern Arc : a bridge between West Asia and Europe

By sequencing 727 ancient individuals from the Southern Arc (Anatolia and its neighbors in Southeastern Europe and West Asia) over 10,000 years, we contextualize its Chalcolithic period and Bronze Age (about 5000 to 1000 BCE), when extensive gene flow entangled it with the Eurasian steppe. Two streams of migration transmitted Caucasus and Anatolian/Levantine ancestry northward, and the Yamnaya pastoralists, formed on the steppe, then spread southward into the Balkans and across the Caucasus into Armenia, where they left numerous patrilineal descendants. Anatolia was transformed by intra–West Asian gene flow, with negligible impact of the later Yamnaya migrations. This contrasts with all other regions where Indo-European languages were spoken, suggesting that the homeland of the Indo-Anatolian language family was in West Asia, with only secondary dispersals of non-Anatolian Indo-Europeans from the steppe