PUESTA EN MARCHA DE UN MODELO IN VITRO 3D DE EPITELIO INTESTINAL COMO BASE DE UN MODELO DE CÁNCER DE COLON EN CHIP

El cáncer de colon es un problema relevante para la salud pública, con una incidencia y una mortalidad elevada en todo el mundo. El desarrollo de nuevos modelos de estudio in vitro ha permitido superar importantes desventajas de modelos anteriores. Entre estos modelos se incluyen los modelos 3D de Organ-On-Chip. El objetivo del presente estudio fue recrear in vitro el epitelio de colon en un dispositivo microfluídico. En dicho modelo las células epiteliales se adhieren a una membrana porosa, que las separa a su vez de un canal inferior. El modelo será la base para el desarrollo posterior de un modelo Organ-On-Chip de cáncer de colon. Para ello se sembraron células Caco-2 o Caco-2:HT29-MTX (a un ratio 3:1, similar al ratio enterocitos:células caliciformes de colon) que se dejaron diferenciar durante 21 días. Sobre el modelo se valoró la diferenciación celular, la integridad del epitelio, la producción de moco y la evolución del epitelio en el tiempo. Se estableció una monocapa tanto con las células Caco-2 como Caco-2:HT29-MTX, que se mantuvo en cultivo durante 21 días. Las pruebas de azul alcián y de inmunofluorescencia para Muc-2 mostraron producción de mucina por las células HT29-MTX a 7 y 21 días, respectivamente. La permeabilidad del epitelio, mediante la técnica de Lucifer Yellow, similar a la observada en intestino y estudios previos, fue ligeramente mayor en las células Caco-2 con HT29-MTX, aunque no significativamente. Los resultados indican la formación de un epitelio similar al del colon, con una permeabilidad parecida y secreción de moco, si bien serán necesarios futuros estudios para una mejor caracterización. <br /

Sense of coherence and quality of life in patients treated with antivitamin k oral anticoagulants: a cross-sectional study

The aim of this study was to analyze the correlation between the participants’ self-reported quality of life and their sense of coherence in a sample (n = 85) of patients on treatment with oral antivitamin K anticoagulants. A cross-sectional design was used. The measurement instruments included a questionnaire on sociodemographic variables, the Spanish version of the Abbreviated World Health Organization Quality of Life questionnaire (WHOQOL-BREF), an oral-anticoagulant-treatment-specific quality-of-life questionnaire, and the sense-of-coherence (SOC) scale. We analyzed the correlations between the participants’ characteristics and the results from the quality-of-life and SOC scales. Age, level of education, employment status, living arrangement, and treatment length were the determinants of the quality of life in people treated with oral anticoagulants. We found a significant association between the four domains of the WHOQOL-BREF questionnaire and general treatment satisfaction (p < 0.01); no significant correlations were found between the SOC subscales and the oral-anticoagulant-treatment-specific quality of life in our sample. Women had a worse level of self-management than men. Nursing interventions should be tailored to the needs of the populations on treatment with oral anticoagulants in order to facilitate a higher level of self-management

An evaluation of undergraduate student nurses' gameful experience whilst playing a digital escape room as part of a FIRST year module: a cross-sectional study

The circumstances arising from the COVID-19 pandemic have accelerated the use of digital teaching and learning in health professions education. Digital gamification-based teaching and learning activities are innovative and versatile tools for the acquisition of professional competencies in higher education, which can be used on a range of topics and can be supplemental to other teaching methods

Psychological distress and fear of Covid-19 in student nurses before clinical placement: a cross-sectional study

Objective: To determine the degree of psychological distress and fear of COVID-19 experienced by undergraduate student nurses who were about to begin their clinical placements. Method: A cross-sectional study was carried out with 100 second- and third-year undergraduate student nurses of the University of Zaragoza (Spain). Measures included the Fear of COVID-19 Scale and the Depression Anxiety Stress Scales. Results: Regularly, student nurses did not think of themselves as vulnerable to COVID-19. However, a significant association was observed between the student nurses’ level of psychological distress and cohabiting with relatives or people who were considered vulnerable to the infection (p = 0.035). The Depression Anxiety Stress Scale results revealed a low level of psychological distress in general; the Fear of COVID-19 Scale indicated moderate fear (2.94). Conclusion: Student nurses who lived with their relatives experienced higher levels of stress due to the perceived risk of transmission, but were less fearful of loss of work and income. Anxiety in our sample was associated principally with not knowing their upcoming placement location

Dependency Theory and the Aesthetics of Contrast in Fernando Solanas’s La hora de los hornos and Memoria del saqueo

This paper is a comparative analysis of two key documentaries by Fernando Solanas: La hora de los hornos / The Hour of the Furnaces (1966–68) and Memoria del saqueo / Social Genocide (2003). It argues that Solanas produces documentaries when the representative link that ties the political representatives to the represented (the people) is suspended or breaks down, as experienced during the times of the proscription of Peronism (1955–73) and in the more recent crisis of representation in Argentine institutional politics (1989–2001). The comparison follows two axes: political arguments and the aesthetics of contrast. Regarding the first criterion, the paper highlights the current persistence, in Solanas’s political argumentation, of externalist-mechanistic versions of dependency theory of the late 1960s and early 1970s. In relation to the aesthetics of contrast, it analyses the stark oppositions in Solanas’s documentaries as a visual rhetoric which can be read as an essentialist false-bottom economy that opposes ‘appearance’ to ‘reality’. The article concludes that these political and aesthetic polarizations are essentializing and literalizing discursive strategies that denounce the excesses of political representation from an unmediated and transparent site of full popular presence. Within such strategies, there is no room for the constitutive opacity intrinsic to representation and articulatory politics

Estudio mixto del distrés psicológico en los alumnos del Grado en Enfermería ante las prácticas curriculares externas durante la pandemia de Covid-19

Los estudiantes universitarios son una población vulnerable de experimentar síntomas de ansiedad, depresión y estrés. Comenzar un nuevo curso académico constituye un reto que, en ocasiones, puede suponer alteraciones en su estado de salud. El comienzo del presente curso académico 2020-2021 está siendo marcado por la pandemia causada por el Coronavirus SARS-CoV-2, lo cual podría acentuar la experiencia de distrés psicológico en los estudiantes universitarios, particularmente en aquellos cuya formación incluye la realización de prácticas clínicas..