Relative proportion of omega 6/3 in the diet of pregnants with the variation of the transgenerational metabolic profile page and incorporation of fatty acids in liver tissue

The essential fatty acids alpha-linolenic acid (α-LNA 18:3 n-3) and linoleic acid (LA 18:2 n-6) fatty acids are precursors of polyunsaturated very long chain (PUFA-VLC) families omega-3 and omega-6. These fatty acids when consumed during pregnancy are important for brain development and adaptation of the retina and fetal tissues, influencing the metabolic pathways of fatty acids in the offspring. Based on this, we seek to verify the relationship of the influence of the ratio of omega 6/3 in the diet of pregnants with the metabolic profile of fatty acids and liver tissue in subsequent generations. For this we used 48 rats (Rattus norvegicus) Wistar, 36 females and 18 males for the founder generation (G0) with the control group (CONT) received a diet with fat source as soybean oil and omega group (OM) received the diet with linseed oil. From these groups, the animals were selected for the training of two generations (F1 and F2). The animals receiving the CONT followed the control diet while the OM group was divided into two new groups, one receiving control diet (OM/CONT) and the other keeping the feed omega (OM/OM). The incorporation of fatty acids (FA) of omega-3 was higher in liver of rats in the group OM, while the FA of omega-6 had a greater uptake of the liver tissue of the CONT group. A major change can be observed in the amount of oleic AG in liver tissue, since the merger occurred higher in the group CONT and the lowest in OM/CONT, demonstrating that the diet provided to the G0 may have somehow influenced the incorporation of generations F1 and F2. This remodeling enzyme was also sufficient to change the outcome of NEFA, which demonstrated decreased lower lipid mobilization in the same group.Os ácidos graxos essenciais alfa-linolênico (α-LNA 18:3n-3) e linoléico (LA 18:2n-6) são precursores de ácidos graxos poli-insaturados de cadeia muito longa (AGPI-CML) das famílias ômega-3 e ômega-6. Estes ácidos graxos quando consumidos durante a gestação são importantes para o desenvolvimento do cérebro e retina e na adaptação de tecidos fetais, influenciando as vias metabólicas dos ácidos graxos na prole. Baseado nisso, buscou-se verificar a relação da influência da proporção ômega 6/3 na dieta da gestante com o perfil metabólico e de ácidos graxos do tecido hepático nas gerações subsequentes. Para isso foram utilizados 48 ratos (Rattus norvegicus) da cepa Wistar/UFPel, sendo 36 fêmeas e 12 machos para a geração fundador (G0), com o grupo controle (CONT) recebendo uma dieta tendo como fonte lipídica o óleo de soja e o grupo ômega (ÔM) que recebeu a dieta com óleo de linhaça. A partir destes grupos, os animais foram selecionados para a formação de mais duas gerações (F1 e F2). Os animais do CONT seguiram recebendo a dieta controle enquanto o grupo ÔM foi dividido em dois novos grupos, um recebendo dieta controle (ÔM/CONT) e o outro mantendo a dieta ômega (ÔM/ÔM). A incorporação de ácidos graxos (AGs) da família ômega-3 foi superior no fígado das ratas do grupo ÔM, enquanto os AGs da família ômega-6 tiveram uma maior incorporação do tecido hepático do grupo CONT. Uma mudança importante pode ser observada na quantidade do AG oléico no tecido hepático, pois a incorporação maior aconteceu no grupo CONT e a menor no ÔM/CONT, demonstrando que a dieta fornecida para a G0 pode ter influenciado de alguma forma a incorporação das gerações F1 e F2. Esta remodelação enzimática foi suficiente também para mudar o resultado do NEFA, que demonstrou menor mobilização lipídica no mesmo grupo

Diets Rich in Polyunsaturated Fatty Acids With Different Omega-6/Omega-3 Ratio Decrease Liver Content of Saturated Fatty Acids Across Generations of Wistar Rats

Our study evaluated how the consumption of diets with low (LOW group - 0.4/1) or high (CON group - 13.6/1) omega-6/omega-3 ratio across generations (F1 and F2) can modulate liver fatty acid (FA) profile and blood biomarkers. Liver content of α-linolenic acid was higher in animals always fed with LOW diet than animals that changed from CON to LOW diet, which by your time was higher than animals always fed with CON diet. Liver saturated FA concentration decreased in both groups from F1 to F2. In conclusion, both diets were efficient in decreasing the saturated FA liver content across generations, the LOW ratio diet was more effective in reducing blood triglycerides and non-esterified fatty acids, and there was a multigenerational effect of the LOW ratio diet, improving the FA profile even when the offspring start receiving the CON diet