Genotype distribution of cervical human papillomavirus DNA in women with cervical lesions in Bioko, Equatorial Guinea

<p>Abstract</p> <p>Background</p> <p>The HVP vaccine is a useful tool for preventing cervical cancer. The purpose of this study is to determine the most frequent HPV genotypes in Equatorial Guinea in order to develop future vaccination strategies to apply in this country.</p> <p>Methods</p> <p>A campaign against cervical cancer was carried out in the area on a total of 1,680 women. 26 of the women, following cytological screening, were treated surgically with a loop electrosurgical excision procedure (LEEP). Cases were studied histologically and were genotyped from paraffin blocks by applying a commercial kit that recognized 35 HPV types.</p> <p>Results</p> <p>Cytological diagnoses included 17 HSIL, 1 LSIL, 5 ASC-H and 3 AGUS. Histological diagnosis resulted in 3 cases of microinvasive squamous cell carcinoma stage IA of FIGO, 9 CIN-3, 8 CIN-2, 2 CIN-1, 3 flat condylomas and mild dysplasia of the endocervical epithelium. Fifteen of twenty-five cases genotyped were positive for HPV (60%). HPV 16 and 33 were identified in four cases each, HPV 58 in two other cases, and HPV 18, 31, 52, and 82 in one case, with one HPV 16 and 58 coinfection.</p> <p>Conclusion</p> <p>The frequency of HPV types in the African area varies in comparison to other regions, particularly in Europe and USA. Vaccination against the five most common HPV types (16, 33, 58, 18, and 31) should be considered in the geographic region of West Africa and specifically in Equatorial Guinea.</p

The review of the Brazilian Forest Act: harmful effects on amphibian conservation

In the last months there is a growing discussion in Brazil about a new Brazilian Forest Act (Código Florestal). This new proposal, in substitution to the current Act, includes modifications which will affect negatively natural populations of amphibians. Besides the deleterious impacts upon amphibians, it will also harm the national and international human populations. Among the possible effects, in consequence of the associate amphibian population declines or complete loss, we cite the increase of agricultural production costs, loss of primary sources of compounds for the medical industry, generalized ecological disequilibrium, eutrofication of water bodies, increase in the costs of water (for humans) treatment, increase of agricultural plagues, and increase of insect borne diseases. All these effects are of high relevance, even more if we consider the population declines of amphibians, the most threatened terrestrial vertebrate group in the world. Therefore, we propose that if a new Forest Act must be prepared, that it should be based, besides in unlikely immediate economical benefits to farm owners, in scientific knowledge which benefits nature conservancy, economy, health, and human welfare.Nos últimos meses está em efervescente discussão uma proposta para que um novo código florestal (Projeto de Lei nº 1876/99) substitua o vigente instaurado por meio da Lei Federal nº 4771/65 e modificado recentemente por Medidas Provisórias. Este novo código proposto, todavia, propõe alterações legais que devem afetar negativamente as populações naturais de anfíbios do Brasil. O declínio de anfíbios deve gerar impactos negativos tanto para a população (humana) nacional, como para a comunidade internacional. Entre os possíveis efeitos dessa lei, em consequência da perda de diversidade de anfíbios, podemos citar o aumento nos custos de produção agrícola, perda de matéria prima para produção de remédios, descontrole ecológico, eutrofização de corpos d'água, encarecimento do custo do tratamento de água para abastecimento humano, aumento de pragas agrícolas e aumento de doenças transmitidas por insetos vetores. Isto tudo é bastante preocupante ainda mais se levarmos em conta a própria perda da biodiversidade de anfíbios, o grupo de vertebrados terrestres mais ameaçado do planeta. Sendo assim, ressaltamos a necessidade de que, caso um novo código florestal seja elaborado, este tenha embasamento também em questões técnicas e científicas que impreterivelmente afetam, não só a conservação da natureza, mas também a economia, saúde e bem estar das populações humanas.3538Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Artistas sobre outras obras

A pós modernidade encerra a interrogação crítica do homem sobre o homem: a linguagem ameaça o que é significante, o que é corpo. O sujeito é falado, e assim constituído depois da linguagem (Althusser, 1976). O jovem Arthur Rimbaud sintetiza-o, aos dezassete anos, numa carta ao seu professor de retórica Georges Izambard, datada de 13 de maio 1871: «C’est faux de dire: je pense: on devrait dire on me pense. Pardon du jeu de mots. Je est un autre». (Rimbaud, 1999: 237) A identidade resiste à linguagem dos homens. A linguagem é transmitida entre as gerações e com ela transporta os sujeitos, ou, o que é dizer o mesmo, a possível interrogação: quem me fala? É esta também a perplexidade que motivou o tema desta revista Estúdio. Reuniram-se, nesta edição, 22 artigos, provenientes de diferentes autores da Argentina, Brasil, Portugal e Espanha, que se debruçam sobre artistas cuja identidade se mostra e merece ser interrogada: são espaços ‘ex-cêntricos,’ para se descobrir.info:eu-repo/semantics/publishedVersio

Variação espacial de cátions, ânions e variáveis físico-químicas no rio Solimões-Amazonas entre Manaus e Jutaí, bacia amazônica

C&#225;tions e &#226;nions majorit&#225;rios s&#227;o esp&#233;cies qu&#237;micas de grande import&#226;ncia nos diversos compartimentos aqu&#225;ticos, por&#233;m, pouco abordados de forma exclusiva na bacia amaz&#244;nica. Neste sentido, o objetivo deste estudo foi avaliar a din&#226;mica espacial de Na+, Ca2+, K+, Mg2+, Cl- e SO4 2- em um trecho do rio Solim&#245;es-Amazonas, levando-se em considera&#231;&#227;o a alcalinidade, potencial hidrogeni&#244;nico (pH), condutividade el&#233;trica (CE), s&#243;lidos totais em suspens&#227;o (STS) e carbono org&#226;nico total (COT). As coletas foram realizadas em maio, julho e setembro de 2012 em sete pontos na calha principal do rio Solim&#245;es-Amazonas e na foz de alguns dos principais tribut&#225;rios: rios Juta&#237;, Juru&#225;, Japur&#225; e Purus. Os c&#225;tions e &#226;nions foram analisados por cromatografia de &#237;ons, STS por gravimetria, pH por potenciometria, alcalinidade por titula&#231;&#227;o potenciom&#233;trica, CE por condutimetria e o COT por oxida&#231;&#227;o catal&#237;tica por combust&#227;o. As concentra&#231;&#245;es m&#233;dias dos c&#225;tions seguiram esta ordem Ca2+>Na+>Mg2+>K+ e dos &#226;nions SO4 2->Cl-. De montante &#224; jusante a tend&#234;ncia foi diminui&#231;&#227;o nas concentra&#231;&#245;es de c&#225;tions e &#226;nions, das vari&#225;veis pH, CE, STS e alcalinidade, al&#233;m do aumento das concentra&#231;&#245;es de COT. O presente estudo evidenciou mudan&#231;as nas caracter&#237;sticas qu&#237;micas sofridas pelo rio Solim&#245;es em seu curso. O aporte dos tribut&#225;rios pode ser apontado como um dos fatores respons&#225;veis por essas modifica&#231;&#245;es

Experiencias en el aula: tercer encuentro de prácticas pedagógicas innovadoras.

Experiencias de profesores en su quehacer en los distintos ambientes de aprendizaje presenciales y a distancia.Para el Centro de Excelencia Docente aeiou constituye un honor presentar la publicación del Tercer Encuentro de Prácticas Pedagógicas Innovadoras en el que se destacan cuarenta trabajos de profesores de UNIMINUTO provenientes de diferentes sedes. Con este encuentro son ya tres que bajo la dirección de aeiou los profesores han compartido su quehacer en los distintos ambientes de aprendizaje presenciales y a distancia. Cada año el Centro de Excelencia Docente invita a los profesores a participar en este evento, para el 2108 además de la inscripción voluntaria por parte de cada profesor, se invitó al estudiantado a que postularan a sus profesores que consideraban eran innovadores y creativos en el cumplimiento de su función docente y se obtuvo una respuesta importante por parte de los estudiantes, que para algunos profesores resultó sorpresiva porque quizás no habían considerado que el trabajo que hacían en su ambiente de aprendizaje era diferente, fuera de lo común. Luego de una evaluación de jurados nacionales e internacionales de las prácticas presentadas y de la realización del evento, que tuvo como novedad hacerlo de forma simultánea en cuatro sedes donde UNIMINUTO tiene presencia: Buga, Ibagué, Pereira y Bogotá, se comparte la presente publicación para tener como referencia y evidencia el trabajo que los profesores hacen a diario

COVID-19 symptoms at hospital admission vary with age and sex: results from the ISARIC prospective multinational observational study

Background: The ISARIC prospective multinational observational study is the largest cohort of hospitalized patients with COVID-19. We present relationships of age, sex, and nationality to presenting symptoms. Methods: International, prospective observational study of 60 109 hospitalized symptomatic patients with laboratory-confirmed COVID-19 recruited from 43 countries between 30 January and 3 August 2020. Logistic regression was performed to evaluate relationships of age and sex to published COVID-19 case definitions and the most commonly reported symptoms. Results: ‘Typical’ symptoms of fever (69%), cough (68%) and shortness of breath (66%) were the most commonly reported. 92% of patients experienced at least one of these. Prevalence of typical symptoms was greatest in 30- to 60-year-olds (respectively 80, 79, 69%; at least one 95%). They were reported less frequently in children (≤ 18 years: 69, 48, 23; 85%), older adults (≥ 70 years: 61, 62, 65; 90%), and women (66, 66, 64; 90%; vs. men 71, 70, 67; 93%, each P &lt; 0.001). The most common atypical presentations under 60 years of age were nausea and vomiting and abdominal pain, and over 60 years was confusion. Regression models showed significant differences in symptoms with sex, age and country. Interpretation: This international collaboration has allowed us to report reliable symptom data from the largest cohort of patients admitted to hospital with COVID-19. Adults over 60 and children admitted to hospital with COVID-19 are less likely to present with typical symptoms. Nausea and vomiting are common atypical presentations under 30 years. Confusion is a frequent atypical presentation of COVID-19 in adults over 60 years. Women are less likely to experience typical symptoms than men

Evaluation of Raw Cheese as a Novel Source of Biofertilizer with a High Level of Biosecurity for Blueberry

Today’s agriculture requires the search for new and modern tools in order to improve and expand the use of its crops and to increase their sustainability. The use of plant growth-promoting PGP bacteria (PGPB) is the target of much research and seems to be an ideal strategy as long as the strains are properly selected for this purpose. Among the bacteria, lactic acid bacteria (LAB) are considered a suitable alternative due to their high biosafety and mechanisms for promoting plant growth. In view of this, in this work we decided to isolate LAB with PGP capacity from raw milk cheese of the PDO “Serra da Estrela”. A total of 88 strains with a high diversity and remarkable capacity to control food-borne and pathogenic microorganisms were isolated. In addition, most of them showed excellent capacities for phosphate solubilization and the production of indole-3-acetic acid and siderophores. Subsequently, we also studied their inoculation in blueberry seedlings. Among the isolates, strains QSE20, QSE62 and QSE79 showed the most remarkable ability to efficiently colonize the rhizosphere of this plant, improving root development and increasing the number of secondary roots

Ciguatoxin detection in flesh and liver of relevant fish species from the Canary Islands

The Canary Islands are a ciguatoxin (CTX) hotspot with an established official monitoring for the detection of CTX in fish flesh from the authorised points of first sale. Fish caught by recreational fishermen are not officially tested and the consumption of toxic viscera or flesh could lead to ciguatera poisoning (CP). The objectives of this study were to determine the presence of CTX-like toxicity in relevant species from this archipelago, compare CTX levels in liver and flesh and examine possible factors involved in their toxicity. Sixty amberjack (Seriola spp.), 27 dusky grouper (Epinephelus marginatus), 11 black moray eels (Muraena helena) and 11 common two-banded seabream (Diplodus vulgaris) were analysed by cell-based assay (CBA) and Caribbean ciguatoxin-1 (C-CTX1) was detected by liquid chromatography mass spectrometry (LC-MS/MS) in all these species. Most of the liver displayed higher CTX levels than flesh and even individuals without detectable CTX in flesh exhibited hepatic toxicity. Black moray eels stand out for the large difference between CTX concentration in both tissues. None of the specimens with non-toxic liver showed toxicity in flesh. This is the first evidence of the presence of C-CTX1 in the common two-banded seabream and the first report of toxicity comparison between liver and muscle from relevant fish species captured in the Canary Islands.European Food Safety Authority | Ref. GP/EFSA/AFSCO/2015/03Ministerio de Educación | Ref. FPU17/04022Ministerio de Ciencia e Innovación | Ref. PID2019-108781RR-C21Ministerio de Ciencia e Innovación | Ref. PID2019 108781RR C22Xunta de Galicia | Ref. ED481A-2018/20