Optimization of a Piezoelectric Mechanical Amplifier Actuator for Nano-Indentation

In the present investigation, a piezo-stack actuator coupled with a flexible diamond shaped frame has been investigated for its mechanical amplification property. The piezo-stack under electrical excitation produces a longitudinal strain along major diagonal of the frame which gets amplified due to its diamond shape along the shorter diagonal. The effect of the thickness of flexural hinges used in the diamond frame on amplification has been studied with a view to optimize the stress and displacement. The effectiveness of the piezoelectric mechanical amplifier actuator as a nano-indenter has been studied and discussed in this paper

Efficient synthesis of pyrene-1-carbothioamides and carboxamides. Tunable solid-state fluorescence of pyrene-1-carboxamides

Pyrene reacts with potassium thiocyanate and organic isothiocyanates in the presence of trifluoromethanesulfonic acid to afford primary and secondary pyrene-1-carbothioamides in high yields. These compounds were efficiently oxidatively desulfurized with Oxone® to the corresponding carboxamides. The amides display solid-state fluorescence with quantum efficiencies up to 62%, originating from monomers, aggregates (such as preformed dimers), and/or excimers, depending on the substituent at the nitrogen atom. Single crystal X-ray diffraction characterization of one highly emissive compound supports this assumption.Publikacja w ramach programu Royal Society of Chemistry "Gold for Gold" 2014 finansowanego przez Uniwersytet Łódzki

Characterization of photophysical and base-mimicking properties of a novel fluorescent adenine analogue in DNA

To increase the diversity of fluorescent base analogues with improved properties, we here present the straightforward click-chemistry-based synthesis of a novel fluorescent adenine-analogue triazole adenine (AT) and its photophysical characterization inside DNA. AT shows promising properties compared to the widely used adenine analogue 2-aminopurine. Quantum yields reach >20% and >5% in single- and double-stranded DNA, respectively, and show dependence on neighbouring bases. Moreover, AT shows only a minor destabilization of DNA duplexes, comparable to 2-aminopurine, and circular dichroism investigations suggest that AT only causes minimal structural perturbations to normal B-DNA. Furthermore, we find that AT shows favourable base-pairing properties with thymine and more surprisingly also with normal adenine. In conclusion, AT shows strong potential as a new fluorescent adenine analogue for monitoring changes within its microenvironment in DNA

Methylated Nucleobases: Synthesis and Evaluation for Base Pairing In Vitro and In Vivo

The synthesis, base pairing properties and in vitro (polymerase) and in vivo (E. coli) recognition of 2′‐deoxynucleotides with a 2‐amino‐6‐methyl‐8‐oxo‐7,8‐dihydro‐purine (X), a 2‐methyl‐6‐thiopurine (Y) and a 6‐methyl‐4‐pyrimidone (Z) base moiety are described. As demonstrated by Tm measurements, the X and Y bases fail to form a self‐complementary base pair. Despite this failure, enzymatic incorporation experiments show that selected DNA polymerases recognize the X nucleotide and incorporate this modified nucleotide versus X in the template. In vivo, X is mainly recognized as a A/G or C base; Y is recognized as a G or C base and Z is mostly recognized as T or C. Replacing functional groups in nucleobases normally involved in W−C recognition (6‐carbonyl and 2‐amino group of purine; 6‐carbonyl of pyrimidine) readily leads to orthogonality (absence of base pairing with natural bases)

Raindrop Size Distribution Characteristics of the Western Pacific Tropical Cyclones Measured in the Palau Islands

Due to the severe threat of tropical cyclones to human life, recent years have witnessed an increase in the investigations on raindrop size distributions of tropical cyclones to improve their quantitative precipitation estimation algorithms and modeling simulations. So far, the raindrop size distributions of tropical cyclones using disdrometer measurements have been conducted at coastal and inland stations, but such studies are still missing for oceanic locations. To the authors’ knowledge, the current study examines—for the first time—the raindrop size distributions of fourteen tropical cyclones observed (during 2003–2007) at an oceanic station, Aimeliik, located in the Palau islands in the Western Pacific. The raindrop size distributions of Western Pacific tropical cyclones measured in the Palau islands showed unlike characteristics between stratiform and convective clusters, with a larger mass-weighted mean diameter and smaller normalized intercept parameter in the convective type. The contribution of the drop diameters to the total number concentration showed a gradual decrease with the increase in drop diameter size. Raindrop size distributions of Western Pacific tropical cyclones measured in the Palau islands differed slightly from Taiwan and Japan. The helpfulness of empirical relations in raindrop size distribution in rainfall estimation algorithms of ground-based (Z–R, μ–Λ, Dm–R, and Nw–R) and remote-sensing (σm–Dm, μo–Dm, Dm–Zku, and Dm–Zka) radars are evaluated. Furthermore, the present study also related the rainfall kinetic energy of fourteen tropical cyclones with rainfall rate and mass-weighted mean diameter (KEtime–R, KEmm–R, and KEmm–Dm). The raindrop size distribution empirical relations appraised in this study offer a chance to: (1) enhance the rain retrieval algorithms of ground-based, remote sensing radars; and (2) improve rainfall kinetic energy estimations using disdrometers and GPM DPR in rainfall erosivity studies

Click chemistry with DNA

The advent of click chemistry has led to an influx of new ideas in the nucleic acids field. The copper catalysed alkyne–azide cycloaddition (CuAAC) reaction is the method of choice for DNA click chemistry due to its remarkable efficiency. It has been used to label oligonucleotides with fluorescent dyes, sugars, peptides and other reporter groups, to cyclise DNA, to synthesise DNA catenanes, to join oligonucleotides to PNA, and to produce analogues of DNA with modified nucleobases and backbones. In this critical review we describe some of the pioneering work that has been carried out in this area (78 references)