Family Reunification among Two Groups of Runaway Adolescents Utilizing Emergency Shelters

Limited research has addressed reunification of runaway youths with their families following an emergency shelter stay; however, recent studies have shown that those who reunify with their families following a shelter stay have more positive outcomes than those relocated to other residences. This study evaluated differences between two samples of runaway youth utilizing youth emergency shelters in New York (n = 155) and Texas (n = 195) and identified factors associated with reunification among these two groups of adolescents. Less than half (43.7%) of the youths were reunited with their families. Among New York runaway youths, those who had lived primarily with someone other than a parent before shelter admission, were physically abused, or neglected were less likely to return home. Among youths admitted to emergency shelter services in Texas, those with longer shelter stays, living primarily with someone other than a parent before shelter admission, or being pregnant or a parent were less likely to reunify. This study provides valuable information concerning family reunification following shelter service use; however, additional research is needed to delineate youth, family, and shelter system factors that distinguish successful from unsuccessful reunification over an extended period of time

Successful Transitions of Runaway/Homeless Youth from Shelter Care

Previous research indicates that runaway and homeless youth often achieve positive outcomes after shelter stays however few studies have examined how these outcomes are achieved. This study employs qualitative methods to explicate this phenomenon. Twenty-five providers and 21 youth from four shelters participated in this study. Youth were recruited who had completed shelter care and returned home for minimally six months. Multiple raters identified themes and created a conceptual model. While in shelter, youths experienced structure and freedom, and the family experienced respite. Once youth became involved in treatment, the family re-connected and the youth returned home. After returning home, youth and family become involved in follow-up services. Results from our study provide insight into the process through which runaway/homeless youth return home after a shelter stay. Our findings emphasize the need for continued change by all members of the family system, highlighting the need for continued intervention to maintain positive changes

Short-Term Outcomes for Youth Receiving Runaway and Homeless Shelter Services

Objective: Few studies have assessed the outcomes of runaway/homeless youth that seek assistance from shelter or crisis services, which would provide much needed documentation of intervention effectiveness and point to new directions for service provision. The goals of the current study were to: (1) assess short-term outcomes among runaway/homeless youth using emergency shelters and crisis services and (2) compare short-term outcomes achieved by runaway/homeless youth in crisis shelters with similar youth using other, longer-term treatment modalities. Method: The study sampled 261 youth using runaway/homeless shelters from four midwestern states at intake and six-weeks postdischarge and 47 high-risk youth using longer-term services at intake and six weeks postintake; ten key outcome variables were assessed. Results: Every outcome variable demonstrated improvement postintervention: days on the run, school suspension and/or detention, and sexual activity decreased; perceived family support and self-esteem increased; and youth were more likely to be currently employed and less likely to have been fired. In comparing runaway/homeless crisis shelter users with day treatment users on the ten outcome variables, there were no significant differences across any of the outcome variables. Conclusions: Despite limitations, the research provides evidence for the short-term effectiveness of crisis shelter services for runaway/homeless youth

Relative distances of Omega Centauri and 47 Tucanae

We present precise optical and near-infrared ground-based photometry of two Globular Clusters (GCs): Omega Cen and 47 Tuc. These photometric catalogs are unbiased in the Red Giant Branch (RGB) region close to the tip. We provide new estimates of the RGB tip (TRGB) magnitudes--m_I(TRGB)=9.84+/-0.05, Omega Cen; m_I(TRGB)=9.46+/-0.06, 47 Tuc--and use these to determine the relative distances of the two GCs. We find that distance ratios based on different calibrations of the TRGB, the RR Lyrae stars and kinematic distances agree with each other within one sigma. Absolute TRGB and RR Lyrae distance moduli agree within 0.10--0.15 mag, while absolute kinematic distance moduli are 0.2--0.3 mag smaller. Absolute distances to 47 Tuc based on the Zero-Age-Horizontal-Branch and on the white dwarf fitting agree within 0.1 mag, but they are 0.1--0.3 mag smaller than TRGB and RR Lyrae distances.Comment: 5 pages, 4 figures, accepted for publication by ApJ

The RMS survey: ammonia mapping of the environment of massive young stellar objects

We present the results of ammonia observations towards 66 massive star forming regions identified by the Red Midcourse Space Experiment Source survey. We have used the Green Bank Telescope and the K-Band Focal Plane Array to map the ammonia (NH3) (1,1) and (2,2) inversion emission at a resolution of 30 arcsec in 8 arcmin regions towards the positions of embedded massive star formation. We have identified a total of 115 distinct clumps, approximately two-thirds of which are associated with an embedded massive young stellar object or compact H ii region, while the others are classified as quiescent. There is a strong spatial correlation between the peak NH3 emission and the presence of embedded objects. We derive the spatial distribution of the kinetic gas temperatures, line widths, and NH3 column densities from these maps, and by combining these data with dust emission maps we estimate clump masses, H2 column densities and ammonia abundances. The clumps have typical masses of ∼1000 M⊙ and radii ∼0.5 pc, line widths of ∼2 km s−1 and kinetic temperatures of ∼16–20 K. We find no significant difference between the sizes and masses of the star-forming and quiescent subsamples; however, the distribution maps reveal the presence of temperature and line width gradients peaking towards the centre for the star-forming clumps while the quiescent clumps show relatively uniform temperatures and line widths throughout. Virial analysis suggests that the vast majority of clumps are gravitationally bound and are likely to be in a state of global free fall in the absence of strong magnetic fields. The similarities between the properties of the two subsamples suggest that the quiescent clumps are also likely to form massive stars in the future, and therefore provide an excellent opportunity to study the initial conditions of massive pre-stellar and protostellar clumps

Parent-of-origin-specific allelic associations among 106 genomic loci for age at menarche.

Age at menarche is a marker of timing of puberty in females. It varies widely between individuals, is a heritable trait and is associated with risks for obesity, type 2 diabetes, cardiovascular disease, breast cancer and all-cause mortality. Studies of rare human disorders of puberty and animal models point to a complex hypothalamic-pituitary-hormonal regulation, but the mechanisms that determine pubertal timing and underlie its links to disease risk remain unclear. Here, using genome-wide and custom-genotyping arrays in up to 182,416 women of European descent from 57 studies, we found robust evidence (P < 5 × 10(-8)) for 123 signals at 106 genomic loci associated with age at menarche. Many loci were associated with other pubertal traits in both sexes, and there was substantial overlap with genes implicated in body mass index and various diseases, including rare disorders of puberty. Menarche signals were enriched in imprinted regions, with three loci (DLK1-WDR25, MKRN3-MAGEL2 and KCNK9) demonstrating parent-of-origin-specific associations concordant with known parental expression patterns. Pathway analyses implicated nuclear hormone receptors, particularly retinoic acid and γ-aminobutyric acid-B2 receptor signalling, among novel mechanisms that regulate pubertal timing in humans. Our findings suggest a genetic architecture involving at least hundreds of common variants in the coordinated timing of the pubertal transition

Biological, clinical and population relevance of 95 loci for blood lipids.

Plasma concentrations of total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol and triglycerides are among the most important risk factors for coronary artery disease (CAD) and are targets for therapeutic intervention. We screened the genome for common variants associated with plasma lipids in &gt;100,000 individuals of European ancestry. Here we report 95 significantly associated loci (P &lt; 5 x 10(-8)), with 59 showing genome-wide significant association with lipid traits for the first time. The newly reported associations include single nucleotide polymorphisms (SNPs) near known lipid regulators (for example, CYP7A1, NPC1L1 and SCARB1) as well as in scores of loci not previously implicated in lipoprotein metabolism. The 95 loci contribute not only to normal variation in lipid traits but also to extreme lipid phenotypes and have an impact on lipid traits in three non-European populations (East Asians, South Asians and African Americans). Our results identify several novel loci associated with plasma lipids that are also associated with CAD. Finally, we validated three of the novel genes-GALNT2, PPP1R3B and TTC39B-with experiments in mouse models. Taken together, our findings provide the foundation to develop a broader biological understanding of lipoprotein metabolism and to identify new therapeutic opportunities for the prevention of CAD

Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples

Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface acoustic wave nebulization (SAWN) is a new technique that has been demonstrated to deposit less energy into ions upon ion formation and transfer for detection than other methods for sample introduction into a mass spectrometer (MS). Here we report the optimization and use of SAWN as a nebulization technique for the introduction of samples from a low flow of liquid, and the interfacing of SAWN with liquid chromatographic separation (LC) for the analysis of a protein digest. This demonstrates that SAWN can be a viable, low-energy alternative to ESI for the LC-MS analysis of proteomic samples

Hundreds of variants clustered in genomic loci and biological pathways affect human height

Most common human traits and diseases have a polygenic pattern of inheritance: DNA sequence variants at many genetic loci influence the phenotype. Genome-wide association (GWA) studies have identified more than 600 variants associated with human traits, but these typically explain small fractions of phenotypic variation, raising questions about the use of further studies. Here, using 183,727 individuals, we show that hundreds of genetic variants, in at least 180 loci, influence adult height, a highly heritable and classic polygenic trait. The large number of loci reveals patterns with important implications for genetic studies of common human diseases and traits. First, the 180 loci are not random, but instead are enriched for genes that are connected in biological pathways (P = 0.016) and that underlie skeletal growth defects (P < 0.001). Second, the likely causal gene is often located near the most strongly associated variant: in 13 of 21 loci containing a known skeletal growth gene, that gene was closest to the associated variant. Third, at least 19 loci have multiple independently associated variants, suggesting that allelic heterogeneity is a frequent feature of polygenic traits, that comprehensive explorations of already-discovered loci should discover additional variants and that an appreciable fraction of associated loci may have been identified. Fourth, associated variants are enriched for likely functional effects on genes, being over-represented among variants that alter amino-acid structure of proteins and expression levels of nearby genes. Our data explain approximately 10% of the phenotypic variation in height, and we estimate that unidentified common variants of similar effect sizes would increase this figure to approximately 16% of phenotypic variation (approximately 20% of heritable variation). Although additional approaches are needed to dissect the genetic architecture of polygenic human traits fully, our findings indicate that GWA studies can identify large numbers of loci that implicate biologically relevant genes and pathways.

XIAP Regulates Cytosol-Specific Innate Immunity to Listeria Infection

The inhibitor of apoptosis protein (IAP) family has been implicated in immune regulation, but the mechanisms by which IAP proteins contribute to immunity are incompletely understood. We show here that X-linked IAP (XIAP) is required for innate immune control of Listeria monocytogenes infection. Mice deficient in XIAP had a higher bacterial burden 48 h after infection than wild-type littermates, and exhibited substantially decreased survival. XIAP enhanced NF-κB activation upon L. monocytogenes infection of activated macrophages, and prolonged phosphorylation of Jun N-terminal kinase (JNK) specifically in response to cytosolic bacteria. Additionally, XIAP promoted maximal production of pro-inflammatory cytokines upon bacterial infection in vitro or in vivo, or in response to combined treatment with NOD2 and TLR2 ligands. Together, our data suggest that XIAP regulates innate immune responses to L. monocytogenes infection by potentiating synergy between Toll-like receptors (TLRs) and Nod-like receptors (NLRs) through activation of JNK- and NF-κB–dependent signaling