Breeding \u3cem\u3eLotus Australis\u3c/em\u3e Andrews for Low Cyanide Content

Lotus australis Andrews is a native perennial tetraploid legume (2n=4x=28) widely distributed throughout Australia (Figure 1). It is highly variable with 14 botanical varieties reported in the Australian Plant Name Index (http://www.anbg.gov.au). Despite broad adaptation within L. australis no cultivars have been developed for cultivation. One of the main barriers to cultivation is the reported cyanogenic nature of the species (Foulds, 1982), which makes it potentially toxic when plant cyanogenic glycosides are fully hydrolysed to form hydrogen cyanide (HCN). Foulds (1982) also reported that the cyanophoric trait was polymorphic at seedling and adult stages with 12% of plants acyanogenic in some populations. . The Cooperative Research Center for Plant-Based Management of Dryland Salinity, financially supported by Australian Wool Innovation has commenced a breeding program to develop a non-toxic cultivar of L. australis. The selection criterion of the first phase of the breeding programme was for low HCN production. Once this trait is stabilised, forage production and seed yield as well as general plant health will be the main breeding objectives

Commercialisation and Impacts of Pasture Legumes in Southern Australia–Lessons Learnt

Forage legumes are a key feature of temperate grasslands in southern Australia, valued for their ability to increase animal production, improve soil fertility and fix atmospheric nitrogen. Of the 36 temperate annual legume and 11 temperate perennial legume species with registered cultivars introduced or domesticated in Australia over the last 100 years, a third have made a major contribution to agriculture, a third have modest use and a third have failed to make any commercial impact. Highly successful species include subterranean clover, barrel medic, white clover, lucerne, French serradella and balansa clover. Species were assessed on the scale of their application, ease of seed production and specific requirements for agronomic management to determine critical factors for maximising commercial success. Of fundamental importance is the need to understand the farming systems context for legume technologies, particularly as it relates to potential scale of application and impact on farm profitability. Other factors included a requirement for parallel investment in rhizobiology, implementing an adequate ‘duty of care’ problem-solving framework for each new plant product and the need to construct a commercialisation model that optimises the trade-off between rapid adoption by farmers and profitability of the seed industry. Our experience to date indicates that seed industry engagement is highest when they have exclusive rights to a cultivar, can exercise some control over seed production and can market seed for a premium price without having to carry over significant seed quantities from one season to the next. A capability for non-specialist seed production on-farm (with lower associated seed costs) is a disincentive for the seed industry, but may be an appropriate commercialisation model for some public cultivars

“I was worried if I don’t have a broken leg they might not take it seriously”: Experiences of men accessing ambulance services for mental health and/or alcohol and other drug problems

Abstract Background A large proportion of ambulance callouts are for men with mental health and/or alcohol and other drug (AOD) problems, but little is known about their experiences of care. This study aimed to describe men's experiences of ambulance care for mental health and/or AOD problems, and factors that influence their care. Methods Interviews were undertaken with 30 men who used an ambulance service for mental health and/or AOD problems in Australia. Interviews were analysed using the Framework approach to thematic analysis. Results Three interconnected themes were abstracted from the data: (a) professionalism and compassion, (b) communication and (c) handover to emergency department staff. Positive experiences often involved paramedics communicating effectively and conveying compassion throughout the episode of care. Conversely, negative experiences often involved a perceived lack of professionalism, and poor communication, especially at handover to emergency department staff. Conclusion Increased training and organizational measures may be needed to enhance paramedics' communication when providing care to men with mental health and/or AOD problems

Improving the Phosphorus Efficiency of Temperate Australian Pastures

Phosphorus (P) is a key input necessary for high production in many temperate, grass-legume pasture systems in Australia because the pastures are situated on P-deficient and moderate to highly P-sorbing soils. A consequence of P-sorption in these soils is that much more P must be applied as fertiliser than will be exported in animal products. The P balance efficiency (PBE=100*Pexport/Pinputs) of grazing enterprises (e.g. wool, meat, milk and live animal export) is about 10-30% and compares poorly with some other agricultural enterprises (e.g. 45-54% for grain production; McLaughlin et al. 1992; Weaver and Wong 2011). P accumulates in these soils when they are fertilised as a result of phosphate reactions with Ca and/or Al and Fe oxides, and P incorporation into resistant organic materials (McLaughlin et al. 2011). Some P in grazed fields is also accumulated in animal camps. The net rate of P accumulation in soil (and in grazed fields as a whole) is related to the concentration of plant-available P in the soil. Operating grazing systems at lower plant-available P levels should help to slow P accumulation and result in more effective use of P fertiliser (Simpson et al. 2010; Simpson et al. 2011). Because the P requirement of grass-legume pastures is usually set by the high P requirements of the legume (Hill et al. 2005), we commenced a study to quantify the P requirements of a range of legumes to determine whether productive, lower P-input grazing systems can be developed. We are also screening subterranean clover, the most widely used pasture legume in temperate Australia, for root traits related to P efficiency. Here we report early findings from the establishment year of a field experiment to determine the P requirement of several alternative temperate legumes

Strengthening the impact of plant genetic resources through collaborative collection, conservation, characterisation, and evaluation: a tribute to the legacy of Dr Clive Francis

This paper is a tribute to the legacy of Dr Clive Francis, who directly and indirectly collected >14 000 accessions across 60 genera of pasture, forage, and crop species and their wild relatives around the Mediterranean basin, Eastern Africa, and Central and South Asia from 1973 to 2005. This was achieved by a collaborative approach that built strong interactions between disparate organisations (ICARDA, VIR, CLIMA, and Australian genebanks) based on germplasm exchange, conservation and documentation, capacity building, and joint collection. These activities greatly strengthened Australian pasture, forage, and crop genebanks, and led to widespread germplasm utilisation that has waned in the last 5 years, reflecting changing priorities among industry funding bodies and research providers. This situation must be reversed, given the pivotal role genetic resource collections must play to broaden the genetic and adaptive base of plant breeding, to meet the challenge of feeding an increasing population in a depleting resource base. Because the use of germplasm subsets that facilitate phenotyping will stimulate wider utilisation of genetic resources, we discuss the application of core collection and germplasm selection through habitat characterisation/filtering in Australian collections. Both are valid entry points into large collections, but the latter has the advantage of enabling both trait discovery and investigation of plant adaptation, and because it is based on a priori hypothesis testing, it increases understanding even when the trait of interest is not identified

Above–belowground herbivore interactions in mixed plant communities are influenced by altered precipitation patterns

Root- and shoot-feeding herbivores have the capacity to influence one another by modifying the chemistry of the shared host plant. This can alter rates of nutrient mineralization and uptake by neighboring plants and influence plant–plant competition, particularly in mixtures combining grasses and legumes. Root herbivory-induced exudation of nitrogen (N) from legume roots, for example, may increase N acquisition by co-occurring grasses, with knock-on effects on grassland community composition. Little is known about how climate change may affect these interactions, but an important and timely question is how will grass–legume mixtures respond in a future with an increasing reliance on legume N mineralization in terrestrial ecosystems. Using a model grass–legume mixture, this study investigated how simultaneous attack on lucerne (Medicago sativa) by belowground weevils (Sitona discoideus) and aboveground aphids (Acyrthosiphon pisum) affected a neighboring grass (Phalaris aquatica) when subjected to drought, ambient, and elevated precipitation. Feeding on rhizobial nodules by weevil larvae enhanced soil water retention under ambient and elevated precipitation, but only when aphids were absent. While drought decreased nodulation and root N content in lucerne, grass root and shoot chemistry were unaffected by changes in precipitation. However, plant communities containing weevils but not aphids showed increased grass height and N concentrations, most likely associated with the transfer of N from weevil-attacked lucerne plants containing more nodules and higher root N concentrations compared with insect-free plants. Drought decreased aphid abundance by 54% but increased total and some specific amino acid concentrations (glycine, lysine, methionine, tyrosine, cysteine, histidine, arginine, aspartate, and glutamate), suggesting that aphid declines were being driven by other facets of drought (e.g., reduced phloem hydraulics). The presence of weevil larvae belowground decreased aphid numbers by 30%, likely associated with a significant reduction in proline in weevil-treated lucerne plants. This study demonstrates how predicted changes to precipitation patterns and indirect interactions between herbivores can alter the outcome of competition between N-fixing legumes and non-N-fixing grasses, with important implications for plant community structure and productivity

Response-based selection of barley cultivars and legume species for complementarity:root morphology and exudation in relation to nutrient source

Phosphorus (P) and nitrogen (N) use efficiency may be improved through increased biodiversity in agroecosystems. Phenotypic variation in plants’ response to nutrient deficiency may influence positive complementarity in intercropping systems. A multicomponent screening approach was used to assess the influence of P supply and N source on the phenotypic plasticity of nutrient foraging traits in barley (H. vulgare L.) and legume species. Root morphology and exudation were determined in six plant nutrient treatments. A clear divergence in the response of barley and legumes to the nutrient treatments was observed. Root morphology varied most among legumes, whereas exudate citrate and phytase activity were most variable in barley. Changes in root morphology were minimized in plants provided with ammonium in comparison to nitrate but increased under P deficiency. Exudate phytase activity and pH varied with legume species, whereas citrate efflux, specific root length, and root diameter lengths were more variable among barley cultivars. Three legume species and four barley cultivars were identified as the most responsive to P deficiency and the most contrasting of the cultivars and species tested. Phenotypic response to nutrient availability may be a promising approach for the selection of plant combinations for minimal input cropping systems