Selected wheat seed defense proteins exhibit competitive binding to model microbial lipid interfaces

Puroindolines (Pins) and purothionins (Pths) are basic, amphiphilic, cysteine-rich wheat proteins that play a role in plant defense against microbial pathogens. We have examined the co-adsorption and sequential addition of Pins (Pin-a, Pin-b and a mutant form of Pin-b with Trp-44 to Arg-44 substitution) and β-purothionin (β-Pth) model anionic lipid layers, using a combination of surface pressure measurements, external reflection FTIR spectroscopy and neutron reflectometry. Results highlighted differences in the protein binding mechanisms, and in the competitive binding and penetration of lipid layers between respective Pins and β-Pth. Pin-a formed a blanket-like layer of protein below the lipid surface that resulted in the reduction or inhibition of β-Pth penetration of the lipid layer. Wild-type Pin-b participated in co-operative binding with β-Pth, whereas the mutant Pin-b did not bind to the lipid layer in the presence of β-Pth. The results provide further insight into the role of hydrophobic and cationic amino acid residues in antimicrobial activity

A domain-specific high-level programming model

International audienceNowadays, computing hardware continues to move toward more parallelism and more heterogeneity, to obtain more computing power. From personal computers to supercomputers, we can find several levels of parallelism expressed by the interconnections of multi-core and many-core accelerators. On the other hand, computing software needs to adapt to this trend, and programmers can use parallel programming models (PPM) to fulfil this difficult task. There are different PPMs available that are based on tasks, directives, or low level languages or library. These offer higher or lower abstraction levels from the architecture by handling their own syntax. However, to offer an efficient PPM with a greater (additional) high-levelabstraction level while saving on performance, one idea is to restrict this to a specific domain and to adapt it to a family of applications. In the present study, we propose a high-level PPM specific to digital signal processing applications. It is based on data-flow graph models of computation, and a dynamic runtime model of execution (StarPU). We show how the user can easily express this digital signal processing application, and can take advantage of task, data and graph parallelism in the implementation, to enhance the performances of targeted heterogeneous clusters composed of CPUs and different accelerators (e.g., GPU, Xeon Phi

Bars in early- and late-type disks in COSMOS

We investigate the (large-scale) bar fraction in a mass-complete sample of M > 10^10.5 Msun disk galaxies at 0.2 < z < 0.6 in the COSMOS field. The fraction of barred disks strongly depends on mass, disk morphology, and specific star formation rate (SSFR). At intermediate stellar mass (10^10.5 < M < 10^11 Msun) the bar fraction in early-type disks is much higher, at all redshifts, by a factor ~2, than that in late-type disks. This trend is reversed at higher stellar mass (M > 10^11 Msun), where the fraction of bars in early-type disks becomes significantly lower, at all redshifts, than that in late-type disks. The bar fractions for galaxies with low and high SSFRs closely follow those of the morphologically-selected early-type and late-type populations, respectively. This indicates a close correspondence between morphology and SSFR in disk galaxies at these earlier epochs. Interestingly, the total bar fraction in 10^10.5 < M < 10^11 Msun disks is built up by a factor of ~2 over the redshift interval explored, while for M > 10^11 Msun disks it remains roughly constant. This indicates that, already by z ~ 0.6, spectral and morphological transformations in the most massive disk galaxies have largely converged to the familiar Hubble sequence that we observe in the local Universe, while for intermediate mass disks this convergence is ongoing until at least z ~ 0.2. Moreover, these results highlight the importance of employing mass-limited samples for quantifying the evolution of barred galaxies. Finally, the evolution of the barred galaxy populations investigated does not depend on the large-scale environmental density (at least, on the scales which can be probed with the available photometric redshifts).Comment: 10 pages, 4 figures, updated to reflect version accepted by MNRA

Assessment of two types of passive sampler for the efficient recovery of SARS-CoV-2 and other viruses from wastewater

Wastewater-based epidemiology (WBE) has proven to be a useful surveillance tool during the ongoing SARS-CoV-2 pandemic, and has driven research into evaluating the most reliable and cost-effective techniques for obtaining a representative sample of wastewater. When liquid samples cannot be taken efficiently, passive sampling approaches have been used, however, insufficient data exists on their usefulness for multi-virus capture and recovery. In this study, we compared the virus-binding capacity of two passive samplers (cotton-based tampons and ion exchange filter papers) in two different water types (deionised water and wastewater). Here we focused on the capture of wastewater-associated viruses including Influenza A and B (Flu-A & B), SARS-CoV-2, human adenovirus (AdV), norovirus GII (NoVGII), measles virus (MeV), pepper mild mottle virus (PMMoV), the faecal marker crAssphage and the process control virus Pseudomonas virus phi6. After deployment, we evaluated four different methods to recover viruses from the passive samplers namely, (i) phosphate buffered saline (PBS) elution followed by polyethylene glycol (PEG) precipitation, (ii) beef extract (BE) elution followed by PEG precipitation, (iii) no-elution into PEG precipitation, and (iv) direct extraction. We found that the tampon-based passive samplers had higher viral recoveries in comparison to the filter paper. Overall, the preferred viral recovery method from the tampon passive samplers was the no-elution/PEG precipitation method. Furthermore, we evidenced that non-enveloped viruses had higher percent recoveries from the passive samplers than enveloped viruses. This is the first study of its kind to assess passive sampler and viral recovery methods amongst a plethora of viruses commonly found in wastewater or used as a viral surrogate in wastewater studies

Denying equality: an analysis of arguments against lowering the age of consent for sex between men

This paper takes a human rights approach to lesbian and gay oppression and critically explores the arguments used to oppose equality in the debates about the age of consent for sex between men. A thematic analysis of Hansard and newspaper reports produced in Britain during the 1990s showed that opponents of the amendment to equalise the age of consent countered with three key arguments laying claim to ethical principles overriding the principle of equality. These were: (1) Principles of right and wrong take precedence over equality; (2) Principles of democracy take precedence over equality; (3) Principles of care and protection take precedence over equality. Two additional arguments (the health risks of anal intercourse, and escalating demands for gay rights) are also outlined. Our findings are discussed with reference to debates on other lesbian and gay rights issues, and we consider the ways in which we might best counter these arguments. </p

Concert recording 2021-11-01

[Track 1]. Triathlon for solo horn. I. Aggressive / Paul Basler -- [Track 2]. Notturno, op. 112 / Carl Reinecke -- [Track 3]. Reflections on a Southern hymn. I. Intonation ; IV. Wondrous love / Stephen Gryc -- [Track 4]. Sunshine for horn, orchestra, and piano / Nathan Pereda -- [Track 5]. Fantasy for horn, op. 88 / Malcolm Arnold-- [Track 6]. Sonata for horn and piano / Margaret Brouwer -- [Track 7]. Tanguito / Dante Yenque -- [Track 8]. Sonata no. 3 for horn and piano / Alec Wilder -- [Track 9]. Aileron for eight horns / James Naigus -- [Track 10]. Six quartets. I. Adagio / Franz Schubert ; arranged by Verne Reynolds -- [Track 11]. Sunshine

Selfgravity and QSO disks

It is well known that the outer parts of QSO accretion disks are prone to selfgravity if heated solely by orbital dissipation. Such disks might be expected to form stars rather than accrete onto the black hole. The arguments leading to this conclusion are reviewed. Conversion of a part of the gas into high-mass stars or stellar-mass black holes, and the release of energy in these objects by fusion or accretion, may help to stabilize the remaining gas. If the disk extends beyond a parsec, however, more energy is probably required for stability than is available by turning half the gas into high-mass stars. Small black holes are perhaps marginally viable energy sources, with important implications (not pursued here) for the QSO spectral energy distribution, the metallicity of the gas, microlensing of QSO disks, and perhaps gravitational-wave searches. Other possible palliatives for selfgravity include accretion driven by nonviscous torques that allow near-sonic accretion speeds and hence lower surface densities for a given mass accretion rate. All such modes of accretion face major theoretical difficulties, and in any case merely postpone selfgravity. Alternatively, thin disks may not exist beyond a thousand Schwarzshild radii or so (0.01 parsec), in which case QSOs must be fueled by gas with small specific angular momentum.Comment: 16 pages, no figures; uses mn2e.cl

The Sun was not born in M 67

Using the most recent proper-motion determination of the old, Solar-metallicity, Galactic open cluster M 67, in orbital computations in a non-axisymmetric model of the Milky Way, including a bar and 3D spiral arms, we explore the possibility that the Sun once belonged to this cluster. We have performed Monte Carlo numerical simulations to generate the present-day orbital conditions of the Sun and M 67, and all the parameters in the Galactic model. We compute 3.5 \times 10^5 pairs of orbits Sun-M 67 looking for close encounters in the past with a minimum distance approach within the tidal radius of M 67. In these encounters we find that the relative velocity between the Sun and M 67 is larger than 20 km/s. If the Sun had been ejected from M 67 with this high velocity by means of a three-body encounter, this interaction would destroy an initial circumstellar disk around the Sun, or disperse its already formed planets. We also find a very low probability, much less than 10^-7, that the Sun was ejected from M 67 by an encounter of this cluster with a giant molecular cloud. This study also excludes the possibility that the Sun and M 67 were born in the same molecular cloud. Our dynamical results convincingly demonstrate that M67 could not have been the birth cluster of our Solar System.Comment: Astronomical Journal accepted (35 pages, 9 figures

Predicting growth and curve progression in the individual patient with adolescent idiopathic scoliosis: design of a prospective longitudinal cohort study

<p>Abstract</p> <p>Background</p> <p>Scoliosis is present in 3-5% of the children in the adolescent age group, with a higher incidence in females. Treatment of adolescent idiopathic scoliosis is mainly dependent on the progression of the scoliotic curve. There is a close relationship between curve progression and rapid (spinal) growth of the patient during puberty. However, until present time no conclusive method was found for predicting the timing and magnitude of the pubertal growth spurt in total body height, or the curve progression of the idiopathic scoliosis.</p> <p>The goal of this study is to determine the predictive value of several maturity indicators that reflect growth or remaining growth potential, in order to predict timing of the peak growth velocity of total body height in the individual patient with adolescent idiopathic scoliosis. Furthermore, different parameters are evaluated for their correlation with curve progression in the individual scoliosis patient.</p> <p>Methods/design</p> <p>This prospective, longitudinal cohort study will be incorporated in the usual care of patients with adolescent idiopathic scoliosis. All new patients between 8 and 17 years with adolescent idiopathic scoliosis (Cobb angle >10 degrees) visiting the outpatient clinic of the University Medical Center Groningen are included in this study. Follow up will take place every 6 months. The present study will use a new ultra-low dose X-ray system which can make total body X-rays. Several maturity indicators are evaluated like different body length dimensions, secondary sexual characteristics, skeletal age in hand and wrist, skeletal age in the elbow, the Risser sign, the status of the triradiate cartilage, and EMG ratios of the paraspinal muscle activity.</p> <p>Correlations of all dimensions will be calculated in relationship to the timing of the pubertal growth spurt, and to the progression of the scoliotic curve. An algorithm will be made for the optimal treatment strategy in the individual patient with adolescent idiopathic scoliosis.</p> <p>Discussion</p> <p>This study will determine the value of many maturity indicators and will be useful as well for other clinicians treating children with disorders of growth. Since not all clinicians have access to the presented new 3D X-ray system or have the time to make EMG's, for example, all indicators will be correlated to the timing of the peak growth velocity of total body height and curve progression in idiopathic scoliosis. Therefore each clinician can chose which indicators can be used best in their practice.</p> <p>Trial registration number</p> <p>NTR2048</p

A simplified extractive metallurgy exercise to demonstrate selective extraction of copper

A laboratory activity is described for senior high school or 1st year undergraduate level students that illustrates key concepts linked to extractive metallurgy. This experiment demonstrates preferential binding of a methoxyphenolic oxime ligand to Cu2+ in the presence of other transition metal ions in aqueous solution. The students are tasked to investigate the importance of the spatial relationship between the oxime and phenol group of two potential ligands by performing a series of short and simple tests. The objective is achieved by identifying which methoxyphenolic oxime ligand selectively and rapidly forms an isolable precipitate with Cu2+ ions. Only one of the potential ligands exhibits a clear preference for binding to Cu2+ and this can easily be identified visually (and confirmed by UV-visible spectroscopy if desired). The experiment has successfully been employed as part of an extracurricular laboratory course involving groups of 25-30 high school students.PostprintPeer reviewe