Assessing the loading and release of metronidazole from bacterial cellulose film as a pharmaceutical dressing

Background: Bacterial cellulose membrane (BCM) produced by Gluconacetobacter xylinus is an advantageous bacterial product and because of its unique properties could be used as an ideal dressing. The aim of this study was to consider the capability of this biomaterial in the release of Metronidazole. In the case of proving this capability, it provides the basis for the production of a dressing containing this type of antibiotic. Materials and Methods: In this study, BCM was initially synthesized by Gluconacetobacter xylinus. The BCM was loaded by Metronidazole. Then the release process was considered in distilled water and buffer phosphate Saline. The ultra violet spectrophotometry was applied for measuring the concentration of the released drug. Results: The chemical structure of bacterial cellulose was confirmed by Fourier Transform Infrared (FT-IR) spectroscopy. The release of Metronidazole in distilled water and phosphate buffered Saline was reached to 84.27 and 84.71, respectively. Due to higher release in phosphate buffered Saline media, it seems that the trend of release in vitro provides efficient results. Conclusion: Results of this study provides the basis for future research on supplying an ideal dressing from this microbial product

Adipose tissue-derived mesenchymal stem cells for breast tissue regeneration

YesWith an escalating incidence of breast cancer cases all over the world and the deleterious psychological impact that mastectomy has on patients along with several limitations of the currently applied modalities, it's plausible to seek unconventional approaches to encounter such a burgeoning issue. Breast tissue engineering may allow that chance via providing more personalized solutions which are able to regenerate, mimicking natural tissues also facing the witnessed limitations. This review is dedicated to explore the utilization of adipose tissue-derived mesenchymal stem cells for breast tissue regeneration among postmastectomy cases focusing on biomaterials and cellular aspects in terms of harvesting, isolation, differentiation and new tissue formation as well as scaffolds types, properties, material–host interaction and an in vitro breast tissue modeling

Pathogenicity locus determinants and toxinotyping of Clostridioides difficile isolates recovered from Iranian patients

Little is known about the toxin profiles, toxinotypes and variations of toxin Clostridioides difficile C (tcdC) in Iranian C. difficile isolates. A total of 818 stool specimens were obtained from outpatients (n = 45) and hospitalized patients (n = 773) in Tehran, Iran, from 2011 to 2017. The 44 C. difficile isolates were subjected to PCR of toxin C. difficile A (tcdA), toxin C. difficile B (tcdB), tcdA 3�-end deletion, toxinotyping and sequencing of the tcdC gene. Thirty-eight isolates (86.36) were identified as tcdA and tcdB positive, and the remaining six isolates (13.63) were nontoxigenic. All tcdA- and tcdB-positive isolates yielded an amplicon of 2535 bp by PCR for the tcdA 3� end. Fourteen (36.84), seventeen (44.73) and seven (18.43) isolates belonged to wild-type, toxin C. difficile C subclone3 (tcdC-sc3) and tcdC-A genotype of tcdC, respectively. Thirty-one isolates (81.57) belonged to toxinotype 0, and seven isolates (18.42) were classified as toxinotype V. This study provides evidence for the circulation of historical and hypervirulent isolates in the healthcare and community settings. Furthermore, it was also demonstrated that the tcdC-A genotype and toxinotype V are not uncommon among Iranian C. difficile isolates. © 201

Study of possibilities of replacement of fish meal by poultry by product in formulated diet of Huso huso

To regard to dramatic decreasing of pelagic fish and depending on fish meal for sturgeon Feeding a project with title on "Study of possibility of replacement of fish meal by poultry by product in formulated diet of Huso huso" in two faz (fingerling) and (Growth out) were carried out. In first faz, proximate composition of fish meal, poultry by product and diet ingredient were determined , thereafter Six test diets (contain 45% protein and 19 Mj kg^-1 energy) were prepared based on anchovy fish meal and poultry by-product substituted at levels of 20% (PBM_20), 40% (PBM_40), 60% (PBM_60), 80% (PBM_80) and 100% (PBM_100), respectively. Huso huso Fingerlings (28.42±0.17gr ± SD) were stocked in 18 fiberglass tanks and fed to satiation to eight week. Result indicated the highest final weight (FW), Weight gain (WG), Specific growth rate (SGR) and Protein efficiency ratio (PER) were belonged to control diet (350.52 ±21.86_gr),(1133.00 ±74.84%),(3.74±0.092 %per day) and (1.58 ±0.017) respectively. But No significant difference was detected between FM, PBM_20, PBM_40 and PBM_60 (P>0.05), Also, there were not significant difference in treatments for Feed efficiency Ratio between (PBM_0), (PBM_20), (PBM_40), (PBM_60) (PBM_80) respectively (P0.05).The best feed efficiency ratio belonged to fish fed PBM_40 that no significant differences with (PBM_20), (PBM_60) and (PBM_80) (P>0.05).a significant decrease of body lipid observed by increasing of supplementation of poultry by product in diets that lowest body lipid (6.6±0.16) were stabilized in (PBM_80) (P0.05). also hepatosomatc index were significantly increase by increasing of poultry by product in diets respectively that highest were observed in fish fed PBM100 (4.2±0.63) (P<0.05).Result of this investigation indicated that poultry by product had high potential for replacing of fish meal in fingerling and growth out Huso huso diet. Therefore suggested a investigation design for research about the effect of poultry by product on growth rate, body composition and immune system of Huso huso at long period for introduce a new replacer product Instead Of fish meal in sturgeon aquaculture industry

Iranian joint registry (iranian national hip and knee arthroplasty registry)

Periodic evaluation and monitoring the health and economic outcome of joint replacement surgery is a common and popular process under the territory of joint registries in many countries. In this article we introduce the methodology used for the foundation of the National Iranian Joint Registry (IJR) with a joint collaboration of the Social Security Organization (SSO) and academic research departments considering the requirements of the Iran's Ministry of Health and Education. ©BY THE ARCHIVES OF BONE AND JOINT SURGERY

Measurement of (anti)deuteron and (anti)proton production in DIS at HERA

The first observation of (anti)deuterons in deep inelastic scattering at HERA has been made with the ZEUS detector at a centre-of-mass energy of 300--318 GeV using an integrated luminosity of 120 pb-1. The measurement was performed in the central rapidity region for transverse momentum per unit of mass in the range 0.3<p_T/M<0.7. The particle rates have been extracted and interpreted in terms of the coalescence model. The (anti)deuteron production yield is smaller than the (anti)proton yield by approximately three orders of magnitude, consistent with the world measurements.Comment: 26 pages, 9 figures, 5 tables, submitted to Nucl. Phys.

Forward jet production in deep inelastic ep scattering and low-x parton dynamics at HERA

Differential inclusive jet cross sections in neutral current deep inelastic ep scattering have been measured with the ZEUS detector. Three phase-space regions have been selected in order to study parton dynamics where the effects of BFKL evolution might be present. The measurements have been compared to the predictions of leading-logarithm parton shower Monte Carlo models and fixed-order perturbative QCD calculations. In the forward region, QCD calculations at order alpha_s^1 underestimate the data up to an order of magnitude at low x. An improved description of the data in this region is obtained by including QCD corrections at order alpha_s^2, which account for the lowest-order t-channel gluon-exchange diagrams, highlighting the importance of such terms in parton dynamics at low x.Comment: 25 pages, 4 figure

Review of management and monitoring of two crop shrimp production of Indian white shrimp Fenneropenaeus indicus in intensive culture system in Guatr Shrimp Farm, Sistan & Baloochestan Province

In line with the implementation of two Crop shrimp culture in year in Gwater shrimp Farming Site credits UNDP aims to increase production of crops per year, reducing the days of culture in the second crop using the nursery pond, control feed conversion ratio (FCR) and production management, Farming of Indian white Shrimp P.indicus in 4 private farm was done in 2008. Surveillance and monitoring of these farms, the possible obstacles to the harmful effects of management strengths and weaknesses and develop in the future was done in corporation of Offshore Fisheries Research Center of Chabahar and fisheries of Sistan and Balouchestan. First crop was successful, but the shrimp of Nursery pond and second crop due to the occurrence of white spot disease (WSSV) disease and casualties were died and did not actually do the work. The average minimum and maximum feed conversion in Culture period 1.46 and 1.96 respectively, in C2 _31 and C2 _ 14 farms were observed. Maximum production was 41,376 kg in farm C2 _31. The rainfall on 14 August severe flooding and water supplying cussed suddenly fall down of Inland channel water salinity at day 16-20 the August to 4-5 PPT and the PH go up to 8.8-9. After 2 days the white spot disease in South of site was confirmed and was transferred immediately to the north of site. (The pilots farm) The important thing was that the farm under two crop system as a last resort so that all First crop harvest but shrimp in Nursery pond were infected and with veterinary supervision of all infected ponds were then killed. The results showed that shrimp farming can be done in two periods in year with a predetermined .In this study the only factor that could have adverse effects on the project was Feed supply problems during the growing period and the consequences that it caused low average body weight and final harvested Shrimp resulted to delay in daily growth