SOURCES OF COPPER INTO THE EUROPEAN AQUATIC ENVIRONMENT.

Chemical contamination from point source discharges in developed (resource-rich) countries has been widely regulated and studied for decades; however, diffuse sources are largely unregulated and widespread. In the European Union, large dischargers report releases of some chemicals; yet, little is known of total emissions (point and diffuse) and their relative significance. We estimated copper loadings from all significant sources including industry, sewage treatment plants, surface runoff (from traffic, architecture and atmospheric deposition), septic tanks, agriculture, mariculture, marine transport (antifoulant leaching), and natural processes. A combination of European datasets, literature and industry data were utilised to generate export coefficients. These were then multiplied by activity rates to derive loads. A total of around 8 kilotons of copper per annum (ktpa) is estimated to enter freshwaters in the European Union and another 3.5 ktpa enters transitional and coastal waters. The main inputs to freshwater are natural processes (3.7 ktpa), agriculture (1.8 ktpa) and runoff (1.8 ktpa). Agricultural emissions are dominated by copper-based plant protection products and farmyard manure. Urban runoff is influenced by copper use in architecture and by vehicle brake linings. Antifoulant leaching from boats (3.2 ktpa) dominates saline water loads of copper. It is noteworthy that most of the emissions originate from a limited number of copper uses where environmental exposure and pathways exist, compared with the bulk of copper use within electrical and electronic equipment and infrastructure that has no environmental pathway during its use. A sensitivity analysis indicated significant uncertainty in data from abandoned mines and urban runoff load estimates. This study provided for the first time a methodology and comprehensive metal load apportionment to European aquatic systems, identifying data gaps and uncertainties which may be refined over time. Source apportionments using this methodology can inform more cost-effective environmental risk assessment and management. This article is protected by copyright. All rights reserved. © 2022 SETAC

Laryngeal manual therapy: a preliminary study to examine its treatment effects in the management of muscle tension dysphonia

The objectives of this study were to determine appropriate acoustic and outcome measures for the evaluation of a method of laryngeal manual therapy (LMT) used in the treatment of patients with muscle tension dysphonia (MTD). The effects of this technique were also investigated. The study was based on the hypotheses that the vertical position of the larynx in the vocal tract would lower, that the quality of the voice would normalize, and that a reduction in any vocal tract discomfort (VTD) would occur after LMT. This was a small, prospective, repeated measures pilot study in which each member of the research team was "blinded" to all other stages of the study and during which all data were anonymized until the final stage of data analysis. Ten subjects presenting with MTD completed outcome measures and provided audiorecordings immediately before, immediately after, and 1 week after LMT. The Kay CSL 4150 was used for signal acquisition and for some acoustic measurements. Spectrographic evaluation was accomplished with Praat. A new perceptual, self-rating scale, the VTD scale, and a new proforma for use by the clinician for palpatory evaluation, were developed for the study. Relative average perturbation during connected speech was significantly reduced after LMT, indicating a reduction in abnormal vocal function. The severity and frequency of VTD was shown to have reduced after LMT. This pilot study showed positive evidence for LMT as a method of therapy in the treatment of hyperfunctional voice disorders. Its effects were shown to be measurable with both acoustical analysis and the VTD scale

Copper transformation, speciation, and detoxification in anoxic and suboxic freshwater sediments

The complex chemistry of copper (Cu) in freshwater sediments at low concentrations is not well understood. We evaluated the transformation processes of Cu added to freshwater sediments under suboxic and anoxic conditions. Freshwater sediments from three sources in Michigan with different characteristics (Spring Creek, River Raisin, and Maple Lake) were spiked with 30 or 60 mg kg-1 Cu and incubated under a nitrogen atmosphere. After 28-d, each treatment subset was amended with organic matter (OM) to promote anoxic conditions and evaluate its effects on Cu speciation. OM addition triggered a shift from suboxic to anoxic conditions, and sequential extractions showed that Cu accordingly shifted from acid-soluble to oxidizable fractions. Extended X-ray absorption fine-structure (EXAFS) spectroscopy revealed that Cu sulfides dominated all anoxic samples except for Spring Creek 30 mg kgxfffd; 1, where Cu(I) was predominantly complexed to thiol groups of OM. Covellite and chalcopyrite (CuFeS2) were the predominant Cu species in nearly all anoxic samples, as determined by Raman spectroscopy, scanning electron microscopy, and X-ray absorption near-edge structure (XANES) spectroscopy. Copper reduction also occurred under suboxic conditions: for two of three sediments, around 80% had been reduced to Cu(I), while the remaining 20% persisted as Cu(II) complexed to OM. However, in the third coarsest (i.e., Spring Creek), around 50% of the Cu had been reduced, forming Cu(I)-OM complexes, while the remainder was Cu(II)-OM complexes. Toxicity tests showed that survival of H. azteca and D. magna were significantly lower in suboxic treatments. Anoxic sediments triggered a near-complete transformation of Cu to sulfide minerals, reducing its toxicity

Effect of Solution Stoichiometry on BaSO4 Crystallization from Turbidity Measurements and Modeling

The impact of solution stoichiometry on the nucleation and growth of BaSO4 was studied by measuring solution transmittance and subsequent fitting to a crystallization model. Our results show that a large excess of either Ba2+ or SO4 2− ions inhibits both the nucleation and growth of BaSO4. However, for a small excess of Ba2+, the growth is enhanced. The dependence of nucleation and growth rates on supersaturation and solution stoichiometry was captured by a semiempirical rate model. Hence, the solution stoichiometry is a highly relevant parameter while studying all aspects of BaSO4 crystallization, and it could be worthwhile to examine other minerals similarly.European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 819588)Junta de Andalucía (PROYEXCEL_00771

Analysis of the symmetry of electrodes for Electropalatography with Cone Beam CT Scanning

The process of compression of air and vibration of activity in the larynx through which speech is produced is of great interest in phonetics, phonology, psychology and is related to various areas of biomedical engineering as it has a strong relationship with cochlear implants, Parkinson’s disease and Stroke. One technique by means of which speech production is analysed is the use of electropalatography, in which an artificial palate, moulded to the speakers’ hard palate is introduced in the mouth. The palate contains a series of electrodes, which monitor contact between the tongue and the palate during speech production. There is interest in the symmetry or asymmetry of the movement of the tongue as this may be related to languages or right- or left-handedness, however this has never been thoroughly studied. A specific limitation of electropalatography for symmetry studies is that palates are hand-crafted and the position of the electrodes themselves may be asymmetric. In this work, we analyse the positioning of electrodes of one electropalatography setting. The symmetry was analysed by locating the electrodes of the palate through the observation of the palate with Computed Tomography. An algorithm to segment the electrodes and find the symmetry of left and right sides of the palates is described. No significant asymmetry was found for one specific palate. The methodology presented should allow the analysis of palates to be used in larger studies of speech production

Effect of Solution Stoichiometry on BaSO4 Crystallization from Turbidity Measurements and Modeling

The impact of solution stoichiometry on the nucleation and growth of BaSO4 was studied by measuring solution transmittance and subsequent fitting to a crystallization model. Our results show that a large excess of either Ba2+ or SO42− ions inhibits both the nucleation and growth of BaSO4. However, for a small excess of Ba2+, the growth is enhanced. The dependence of nucleation and growth rates on supersaturation and solution stoichiometry was captured by a semiempirical rate model. Hence, the solution stoichiometry is a highly relevant parameter while studying all aspects of BaSO4 crystallization, and it could be worthwhile to examine other minerals similarly

A walk in the PARC:developing and implementing 21st century chemical risk assessment in Europe

Current approaches for the assessment of environmental and human health risks due to exposure to chemical substances have served their purpose reasonably well. Nevertheless, the systems in place for different uses of chemicals are faced with various challenges, ranging from a growing number of chemicals to changes in the types of chemicals and materials produced. This has triggered global awareness of the need for a paradigm shift, which in turn has led to the publication of new concepts for chemical risk assessment and explorations of how to translate these concepts into pragmatic approaches. As a result, next-generation risk assessment (NGRA) is generally seen as the way forward. However, incorporating new scientific insights and innovative approaches into hazard and exposure assessments in such a way that regulatory needs are adequately met has appeared to be challenging. The European Partnership for the Assessment of Risks from Chemicals (PARC) has been designed to address various challenges associated with innovating chemical risk assessment. Its overall goal is to consolidate and strengthen the European research and innovation capacity for chemical risk assessment to protect human health and the environment. With around 200 participating organisations from all over Europe, including three European agencies, and a total budget of over 400 million euro, PARC is one of the largest projects of its kind. It has a duration of seven years and is coordinated by ANSES, the French Agency for Food, Environmental and Occupational Health & Safety

Identification of a Common Gene Expression Response in Different Lung Inflammatory Diseases in Rodents and Macaques

To identify gene expression responses common to multiple pulmonary diseases we collected microarray data for acute lung inflammation models from 12 studies and used these in a meta-analysis. The data used include exposures to air pollutants; bacterial, viral, and parasitic infections; and allergic asthma models. Hierarchical clustering revealed a cluster of 383 up-regulated genes with a common response. This cluster contained five subsets, each characterized by more specific functions such as inflammatory response, interferon-induced genes, immune signaling, or cell proliferation. Of these subsets, the inflammatory response was common to all models, interferon-induced responses were more pronounced in bacterial and viral models, and a cell division response was more prominent in parasitic and allergic models. A common cluster containing 157 moderately down-regulated genes was associated with the effects of tissue damage. Responses to influenza in macaques were weaker than in mice, reflecting differences in the degree of lung inflammation and/or virus replication. The existence of a common cluster shows that in vivo lung inflammation in response to various pathogens or exposures proceeds through shared molecular mechanisms

Prevalence and determinants of human papillomavirus genital infection in men

Four-hundred-forty-five husbands of women with invasive cervical carcinoma, 165 of women with in situ cervical cancer, and 717 of control women (age range 19–82 years) were interviewed and a sample of exfoliated cells from the penis obtained in seven case–control studies conducted by the International Agency for Research on Cancer. The characteristics of human papillomavirus-positive and human papillomavirus-negative husbands were compared using odds ratios and 95% confidence intervals. Thirteen per cent of the husbands of control women, 18% of the husbands of women with invasive cervical carcinoma, and 21% of the husbands of in situ cervical carcinoma women were positive for penile human papillomavirus DNA. Human papillomavirus 16 was detected in 45 husbands, human papillomavirus 18, 31 or 33 in 19, and human papillomavirus 6/11 in 6, but the majority of human papillomavirus infection (158) was with other or unspecified human papillomavirus types. The same human papillomavirus type was seldom identified in both husband and wife. The strongest variation in penile human papillomavirus infection was by country, with percentages among the husbands of control women ranging between 3% in Spain and 39% in Brazil. Having had over 50 lifetime sexual partners, compared with only one, was associated with an odds ratio of 2.3

Elemental Composition of Natural Nanoparticles and Fine Colloids in European Forest Stream Waters and Their Role as Phosphorus Carriers

"This is the peer reviewed version of the following article: Gottselig, N., W. Amelung, J. W. Kirchner, R. Bol, W. Eugster, S. J. Granger, C. Hernández-Crespo, et al. 2017. Elemental Composition of Natural Nanoparticles and Fine Colloids in European Forest Stream Waters and Their Role as Phosphorus Carriers. Global Biogeochemical Cycles 31 (10). American Geophysical Union (AGU): 1592 1607. doi:10.1002/2017gb005657, which has been published in final form at https://doi.org/10.1002/2017GB005657. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."[EN] Biogeochemical cycling of elements largely occurs in dissolved state, but many elements may also be bound to natural nanoparticles (NNP, 1-100 nm) and fine colloids (100-450 nm). We examined the hypothesis that the size and composition of stream water NNP and colloids vary systematically across Europe. To test this hypothesis, 96 stream water samples were simultaneously collected in 26 forested headwater catchments along two transects across Europe. Three size fractions (similar to 1-20 nm, >20-60 nm, and >60 nm) of NNP and fine colloids were identified with Field Flow Fractionation coupled to inductively coupled plasma mass spectrometry and an organic carbon detector. The results showed that NNP and fine colloids constituted between 2 +/- 5% (Si) and 53 +/- 21% (Fe; mean +/- SD) of total element concentrations, indicating a substantial contribution of particles to element transport in these European streams, especially for P and Fe. The particulate contents of Fe, Al, and organic C were correlated to their total element concentrations, but those of particulate Si, Mn, P, and Ca were not. The fine colloidal fractions >60 nm were dominated by clay minerals across all sites. The resulting element patterns of NNP <60 nm changed from North to South Europe from Fe-to Ca-dominated particles, along with associated changes in acidity, forest type, and dominant lithology.The authors gratefully acknowledge the assistance of the following people in locating suitable sampling sites, contacting site operators, performing the sampling, and providing data: A. Avila Castells (Autonomous University of Barcelona), R. Batalla (University of Lleida), P. Blomkvist (Swedish University of Agricultural Sciences), H. Bogena (Julich Research Center), A.K. Boulet (University of Aveiro), D. Estany (University of Lleida), F. Garnier (French National Institute of Agricultural Research), H.J. Hendricks-Franssen (Research Center Julich), L. JacksonBlake (James Hutton Institute, NIVA), T. Laurila (Finnish Meteorological Institute), A. Lindroth (Lund University), M.M. Monerris (Universitat Politecnica de Valencia), M. Ottosson Lofvenius (Swedish University of Agricultural Sciences), I. Taberman (Swedish University of Agricultural Sciences), F. Wendland (Research Center Julich), T. Zetterberg (Swedish University of Agricultural Sciences and The Swedish Environmental Research Institute, IVL) and further unnamed contributors. The Swedish Infrastructure for Ecosystem Science (SITES) and the Swedish Integrated Monitoring, the latter financed by the Swedish Environmental Protection Agency, and ICOS Sweden have supported sampling and provided data for the Swedish sites. J.J.K. gratefully acknowledges the support from CESAM (UID/AMB/50017/2013), funded by the FCT/MCTES (PIDDAC) with cofunding by FEDER through COMPETE. N.G. gratefully acknowledges all those who contributed to organizing and implementing the continental sampling. The raw data can be found at http://hdl.handle.net/2128/14937. This project was partly funded by the German Research Foundation (DFG KL2495/1-1).Gottselig, N.; Amelung, W.; Kirchner, J.; Bol, R.; Eugster, W.; Granger, S.; Hernández Crespo, C.... (2017). Elemental Composition of Natural Nanoparticles and Fine Colloids in European Forest Stream Waters and Their Role as Phosphorus Carriers. Global Biogeochemical Cycles. 31(10):1592-1607. https://doi.org/10.1002/2017GB005657S159216073110Baken, S., Moens, C., van der Grift, B., & Smolders, E. (2016). Phosphate binding by natural iron-rich colloids in streams. Water Research, 98, 326-333. doi:10.1016/j.watres.2016.04.032Baken, S., Regelink, I. C., Comans, R. N. J., Smolders, E., & Koopmans, G. F. (2016). Iron-rich colloids as carriers of phosphorus in streams: A field-flow fractionation study. Water Research, 99, 83-90. doi:10.1016/j.watres.2016.04.060Benedetti, M. F., Van Riemsdijk, W. H., Koopal, L. K., Kinniburgh, D. G., Gooddy, D. C., & Milne, C. J. (1996). Metal ion binding by natural organic matter: From the model to the field. Geochimica et Cosmochimica Acta, 60(14), 2503-2513. doi:10.1016/0016-7037(96)00113-5Binkley, D., Ice, G. G., Kaye, J., & Williams, C. A. (2004). NITROGEN AND PHOSPHORUS CONCENTRATIONS IN FOREST STREAMS OF THE UNITED STATES. Journal of the American Water Resources Association, 40(5), 1277-1291. doi:10.1111/j.1752-1688.2004.tb01586.xBishop, K., Buffam, I., Erlandsson, M., Fölster, J., Laudon, H., Seibert, J., & Temnerud, J. (2008). Aqua Incognita: the unknown headwaters. Hydrological Processes, 22(8), 1239-1242. doi:10.1002/hyp.7049Bol, R., Julich, D., Brödlin, D., Siemens, J., Kaiser, K., Dippold, M. A., … Hagedorn, F. (2016). Dissolved and colloidal phosphorus fluxes in forest ecosystems-an almost blind spot in ecosystem research. Journal of Plant Nutrition and Soil Science, 179(4), 425-438. doi:10.1002/jpln.201600079Buffle, J., & Leppard, G. G. (1995). Characterization of Aquatic Colloids and Macromolecules. 2. Key Role of Physical Structures on Analytical Results. Environmental Science & Technology, 29(9), 2176-2184. doi:10.1021/es00009a005Celi, L., & Barberis, E. (s. f.). Abiotic stabilization of organic phosphorus in the environment. Organic phosphorus in the environment, 113-132. doi:10.1079/9780851998220.0113Dahlqvist, R., Benedetti, M. F., Andersson, K., Turner, D., Larsson, T., Stolpe, B., & Ingri, J. (2004). Association of calcium with colloidal particles and speciation of calcium in the Kalix and Amazon rivers. Geochimica et Cosmochimica Acta, 68(20), 4059-4075. doi:10.1016/j.gca.2004.04.007Darch, T., Blackwell, M. S. A., Hawkins, J. M. B., Haygarth, P. M., & Chadwick, D. (2014). A Meta-Analysis of Organic and Inorganic Phosphorus in Organic Fertilizers, Soils, and Water: Implications for Water Quality. Critical Reviews in Environmental Science and Technology, 44(19), 2172-2202. doi:10.1080/10643389.2013.790752Dynesius, M., & Nilsson, C. (1994). Fragmentation and Flow Regulation of River Systems in the Northern Third of the World. Science, 266(5186), 753-762. doi:10.1126/science.266.5186.753Erickson, H. P. (2009). Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy. Biological Procedures Online, 11(1), 32-51. doi:10.1007/s12575-009-9008-xEspinosa, M., Turner, B. L., & Haygarth, P. M. (1999). Preconcentration and Separation of Trace Phosphorus Compounds in Soil Leachate. Journal of Environmental Quality, 28(5), 1497-1504. doi:10.2134/jeq1999.00472425002800050015xFernández-Martínez, M., Vicca, S., Janssens, I. A., Sardans, J., Luyssaert, S., Campioli, M., … Peñuelas, J. (2014). Nutrient availability as the key regulator of global forest carbon balance. Nature Climate Change, 4(6), 471-476. doi:10.1038/nclimate2177Giddings, J., Yang, F., & Myers, M. (1976). Flow-field-flow fractionation: a versatile new separation method. Science, 193(4259), 1244-1245. doi:10.1126/science.959835Gimbert, L. J., Andrew, K. N., Haygarth, P. M., & Worsfold, P. J. (2003). Environmental applications of flow field-flow fractionation (FIFFF). TrAC Trends in Analytical Chemistry, 22(9), 615-633. doi:10.1016/s0165-9936(03)01103-8Gottselig, N., Bol, R., Nischwitz, V., Vereecken, H., Amelung, W., & Klumpp, E. (2014). Distribution of Phosphorus-Containing Fine Colloids and Nanoparticles in Stream Water of a Forest Catchment. Vadose Zone Journal, 13(7), vzj2014.01.0005. doi:10.2136/vzj2014.01.0005Gottselig, N., Nischwitz, V., Meyn, T., Amelung, W., Bol, R., Halle, C., … Klumpp, E. (2017). Phosphorus Binding to Nanoparticles and Colloids in Forest Stream Waters. Vadose Zone Journal, 16(3), vzj2016.07.0064. doi:10.2136/vzj2016.07.0064Hagedorn , A. G. 2006 EG-Sicherheitsdatenblatt (Gemäß 2001/58/EG)Hart, B. T., Douglas, G. B., Beckett, R., Van Put, A., & Van Grieken, R. E. (1993). Characterization of colloidal and particulate matter transported by the magela creek system, Northern Australia. Hydrological Processes, 7(1), 105-118. doi:10.1002/hyp.3360070111Hassellöv, M., Lyvén, B., Haraldsson, C., & Sirinawin, W. (1999). Determination of Continuous Size and Trace Element Distribution of Colloidal Material in Natural Water by On-Line Coupling of Flow Field-Flow Fractionation with ICPMS. Analytical Chemistry, 71(16), 3497-3502. doi:10.1021/ac981455yHassellov, M., & von der Kammer, F. (2008). Iron Oxides as Geochemical Nanovectors for Metal Transport in Soil-River Systems. Elements, 4(6), 401-406. doi:10.2113/gselements.4.6.401Hens, M., & Merckx, R. (2001). Functional Characterization of Colloidal Phosphorus Species in the Soil Solution of Sandy Soils. Environmental Science & Technology, 35(3), 493-500. doi:10.1021/es0013576Hill, D. M., & Aplin, A. C. (2001). Role of colloids and fine particles in the transport of metals in rivers draining carbonate and silicate terrains. Limnology and Oceanography, 46(2), 331-344. doi:10.4319/lo.2001.46.2.0331Jarvie, H. P., Neal, C., Rowland, A. P., Neal, M., Morris, P. N., Lead, J. R., … Hockenhull, K. (2012). Role of riverine colloids in macronutrient and metal partitioning and transport, along an upland–lowland land-use continuum, under low-flow conditions. Science of The Total Environment, 434, 171-185. doi:10.1016/j.scitotenv.2011.11.061Jiang, X., Bol, R., Nischwitz, V., Siebers, N., Willbold, S., Vereecken, H., … Klumpp, E. (2015). Phosphorus Containing Water Dispersible Nanoparticles in Arable Soil. Journal of Environmental Quality, 44(6), 1772-1781. doi:10.2134/jeq2015.02.0085Kögel-Knabner, I., & Amelung, W. (2014). Dynamics, Chemistry, and Preservation of Organic Matter in Soils. Treatise on Geochemistry, 157-215. doi:10.1016/b978-0-08-095975-7.01012-3Krám, P., Hruška, J., & Shanley, J. B. (2012). Streamwater chemistry in three contrasting monolithologic Czech catchments. Applied Geochemistry, 27(9), 1854-1863. doi:10.1016/j.apgeochem.2012.02.020Lyvén, B., Hassellöv, M., Turner, D. R., Haraldsson, C., & Andersson, K. (2003). Competition between iron- and carbon-based colloidal carriers for trace metals in a freshwater assessed using flow field-flow fractionation coupled to ICPMS. Geochimica et Cosmochimica Acta, 67(20), 3791-3802. doi:10.1016/s0016-7037(03)00087-5Marschner, B., & Kalbitz, K. (2003). Controls of bioavailability and biodegradability of dissolved organic matter in soils. Geoderma, 113(3-4), 211-235. doi:10.1016/s0016-7061(02)00362-2Martin, J.-M., Dai, M.-H., & Cauwet, G. (1995). Significance of colloids in the biogeochemical cycling of organic carbon and trace metals in the Venice Lagoon (Italy). Limnology and Oceanography, 40(1), 119-131. doi:10.4319/lo.1995.40.1.0119Mattsson, T., Kortelainen, P., Laubel, A., Evans, D., Pujo-Pay, M., Räike, A., & Conan, P. (2009). Export of dissolved organic matter in relation to land use along a European climatic gradient. Science of The Total Environment, 407(6), 1967-1976. doi:10.1016/j.scitotenv.2008.11.014Missong, A., Bol, R., Willbold, S., Siemens, J., & Klumpp, E. (2016). Phosphorus forms in forest soil colloids as revealed by liquid-state31P-NMR. Journal of Plant Nutrition and Soil Science, 179(2), 159-167. doi:10.1002/jpln.201500119Montalvo, D., Degryse, F., & McLaughlin, M. J. (2015). Natural Colloidal P and Its Contribution to Plant P Uptake. Environmental Science & Technology, 49(6), 3427-3434. doi:10.1021/es504643fNeubauer, E., Köhler, S. J., von der Kammer, F., Laudon, H., & Hofmann, T. (2013). Effect of pH and Stream Order on Iron and Arsenic Speciation in Boreal Catchments. Environmental Science & Technology, 47(13), 7120-7128. doi:10.1021/es401193jNeubauer, E., v.d. Kammer, F., & Hofmann, T. (2011). Influence of carrier solution ionic strength and injected sample load on retention and recovery of natural nanoparticles using Flow Field-Flow Fractionation. Journal of Chromatography A, 1218(38), 6763-6773. doi:10.1016/j.chroma.2011.07.010Nischwitz, V., & Goenaga-Infante, H. (2012). Improved sample preparation and quality control for the characterisation of titanium dioxide nanoparticles in sunscreens using flow field flow fractionation on-line with inductively coupled plasma mass spectrometry. Journal of Analytical Atomic Spectrometry, 27(7), 1084. doi:10.1039/c2ja10387gRan, Y., Fu, J. ., Sheng, G. ., Beckett, R., & Hart, B. . (2000). Fractionation and composition of colloidal and suspended particulate materials in rivers. Chemosphere, 41(1-2), 33-43. doi:10.1016/s0045-6535(99)00387-2Regelink, I. C., Koopmans, G. F., van der Salm, C., Weng, L., & van Riemsdijk, W. H. (2013). Characterization of Colloidal Phosphorus Species in Drainage Waters from a Clay Soil Using Asymmetric Flow Field-Flow Fractionation. Journal of Environmental Quality, 42(2), 464-473. doi:10.2134/jeq2012.0322Regelink, I. C., Voegelin, A., Weng, L., Koopmans, G. F., & Comans, R. N. J. (2014). Characterization of Colloidal Fe from Soils Using Field-Flow Fractionation and Fe K-Edge X-ray Absorption Spectroscopy. Environmental Science & Technology, 48(8), 4307-4316. doi:10.1021/es405330xRegelink, I. C., Weng, L., & van Riemsdijk, W. H. (2011). The contribution of organic and mineral colloidal nanoparticles to element transport in a podzol soil. Applied Geochemistry, 26, S241-S244. doi:10.1016/j.apgeochem.2011.03.114RICHARDSON, C. J. (1985). Mechanisms Controlling Phosphorus Retention Capacity in Freshwater Wetlands. Science, 228(4706), 1424-1427. doi:10.1126/science.228.4706.1424Roth , C. 2011 Sicherheitsdatenblatt Gemäß Verordnung (EG) Nr. 1907/2006 RepSchmitt, D., Taylor, H. E., Aiken, G. R., Roth, D. A., & Frimmel, F. H. (2002). Influence of Natural Organic Matter on the Adsorption of Metal Ions onto Clay Minerals. Environmental Science & Technology, 36(13), 2932-2938. doi:10.1021/es010271pSix, J., Elliott, E. T., & Paustian, K. (1999). Aggregate and Soil Organic Matter Dynamics under Conventional and No-Tillage Systems. Soil Science Society of America Journal, 63(5), 1350-1358. doi:10.2136/sssaj1999.6351350xStolpe, B., Guo, L., Shiller, A. M., & Hassellöv, M. (2010). Size and composition of colloidal organic matter and trace elements in the Mississippi River, Pearl River and the northern Gulf of Mexico, as characterized by flow field-flow fractionation. Marine Chemistry, 118(3-4), 119-128. doi:10.1016/j.marchem.2009.11.007Tipping, E., & Hurley, M. . (1992). A unifying model of cation binding by humic substances. Geochimica et Cosmochimica Acta, 56(10), 3627-3641. doi:10.1016/0016-7037(92)90158-fTombácz, E., Libor, Z., Illés, E., Majzik, A., & Klumpp, E. (2004). The role of reactive surface sites and complexation by humic acids in the interaction of clay mineral and iron oxide particles. Organic Geochemistry, 35(3), 257-267. doi:10.1016/j.orggeochem.2003.11.002Trostle, K. D., Ray Runyon, J., Pohlmann, M. A., Redfield, S. E., Pelletier, J., McIntosh, J., & Chorover, J. (2016). Colloids and organic matter complexation control trace metal concentration-discharge relationships in Marshall Gulch stream waters. Water Resources Research, 52(10), 7931-7944. doi:10.1002/2016wr019072U.S. Department of Agriculture 1993 Soil survey manual, chapter 3. Selected chemical propertiesVitousek, P. (1982). Nutrient Cycling and Nutrient Use Efficiency. The American Naturalist, 119(4), 553-572. doi:10.1086/283931Wells, M. L., & Goldberg, E. D. (1991). Occurrence of small colloids in sea water. Nature, 353(6342), 342-344. doi:10.1038/353342a0Wen, L.-S., Santschi, P., Gill, G., & Paternostro, C. (1999). Estuarine trace metal distributions in Galveston Bay: importance of colloidal forms in the speciation of the dissolved phase. Marine Chemistry, 63(3-4), 185-212. doi:10.1016/s0304-4203(98)00062-0Zirkler, D., Lang, F., & Kaupenjohann, M. (2012). «Lost in filtration»—The separation of soil colloids from larger particles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 399, 35-40. doi:10.1016/j.colsurfa.2012.02.02