Comunicar, colaborar, aprender: el caso de la intranet de la Biblioteca de la Universidad de Burgos

Comunicación presentada en las 4as. Jornadas BUCLE sobre bibliotecas universitarias, celebradas en Ávila los días 16 y 17 de septiembre de 2010.[ES] Desde el año 2007 la Biblioteca de la Universidad de Burgos dispone de una nueva intranet corporativa que está planteada como una herramienta para la gestión automatizada de las acciones que componen los 9 criterios del modelo EFQM. Sus objetivos son facilitar la comunicación interna y el trabajo colaborativo mejorando el flujo de la información, de forma que sea posible un proceso continuo de aprendizaje, innovación y mejora. Consta de los siguientes módulos: Gestión documental, Gestión de procedimientos, Gestión de indicadores, Gestión de incidencias, Agenda de eventos y Foros de discusión

The impact of manual patient handling on work ability: A cross-sectional study in a Brazilian hospital

Aim: To investigate the level of self-reported work ability and its association with manual patient handling in healthcare workers. Design: Cross-sectional study adhering to the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines. Methods: A total of 320 healthcare workers answered a self-administered questionnaire regarding manual patient handling, work ability, occupational factors, occurrence of low back pain and sociodemographic and lifestyle factors from November 2016 to March 2017. The association between manual patient handling and low back pain was analysed with Poisson regression models. Results: The prevalence ratio of inadequate work ability was 43.42%. Manual patient handling (PR 1.375, 95% CI 1.038–1.821), bachelor education (PR 2.150, 95% CI 1.272–3.632), less than bachelor education (PR 2.166, 95% CI 1.218–3.855), seniority (PR 1.049, 95% CI 1.024–1.086), poor sleep quality (PR 1.425, 95% CI 1.13–1.796) and presence of low back pain (PR 2.003, 95% CI 1.314–3.052) were all positively associated with an inadequate work ability.Funding for open access charge: Universidad de Huelva/CBU

Stabilization of Lactobacillus sp. with enhanced thermal resistance by spray-drying

[EN] Combining biotechnology and encapsulation by spray-drying is possible to obtain microorganisms-based products in powder format without losing viability. A methodology based on the improvement of the thermal stress resistance of selected strains of Lactobacillus sp. and subsequent culture stabilization by spray-drying by optimizing the process parameters and using thermal protectant materials has been developed. The results obtained showed that the final product kept the viability of the initial culture in addition to be a solid, powdery and ease-to-handle product.Rivera, D.; Valverde, M.; Valera, A.; Torrejón, A.; Espí, J.; Gómez, E.; Ruiz, B. (2018). Stabilization of Lactobacillus sp. with enhanced thermal resistance by spray-drying. En IDS 2018. 21st International Drying Symposium Proceedings. Editorial Universitat Politècnica de València. 1341-1348. https://doi.org/10.4995/IDS2018.2018.7625OCS1341134

Metabolic adaptations in spontaneously immortalized PGC-1α knock-out mouse embryonic fibroblasts increase their oncogenic potential

PGC-1α controls, to a large extent, the capacity of cells to respond to changing nutritional requirements and energetic demands. The key role of metabolic reprogramming in tumor development has highlighted the potential role of PGC-1α in cancer. To investigate how loss of PGC-1α activity in primary cells impacts the oncogenic characteristics of spontaneously immortalized cells, and the mechanisms involved, we used the classic 3T3 protocol to generate spontaneously immortalized mouse embryonic fibroblasts (iMEFs) from wild-type (WT) and PGC-1α knockout (KO) mice and analyzed their oncogenic potential in vivo and in vitro. We found that PGC-1α KO iMEFs formed larger and more proliferative primary tumors than WT counterparts, and fostered the formation of lung metastasis by B16 melanoma cells. These characteristics were associated with the reduced capacity of KO iMEFs to respond to cell contact inhibition, in addition to an increased ability to form colonies in soft agar, an enhanced migratory capacity, and a reduced growth factor dependence. The mechanistic basis of this phenotype is likely associated with the observed higher levels of nuclear β-catenin and c-myc in KO iMEFs. Evaluation of the metabolic adaptations of the immortalized cell lines identified a decrease in oxidative metabolism and an increase in glycolytic flux in KO iMEFs, which were also more dependent on glutamine for their survival. Furthermore, glucose oxidation and tricarboxylic acid cycle forward flux were reduced in KO iMEF, resulting in the induction of compensatory anaplerotic pathways. Indeed, analysis of amino acid and lipid patterns supported the efficient use of tricarboxylic acid cycle intermediates to synthesize lipids and proteins to support elevated cell growth rates. All these characteristics have been observed in aggressive tumors and support a tumor suppressor role for PGC-1α, restraining metabolic adaptations in cancer.This work was funded by grants from the Spanish “Ministerio de Ciencia, Innovación y Universidades” (MICINN) and ERDF/FEDER funds, SAF2012-37693, SAF2015-63904-R, SAF2015-71521-REDC, RTI2018-093864-B-I00 to M.M., SAF2017-83043-R and B2017/BMD-3724 to S·C., PI15/00107 to A.M.R, the University of the Basque Country UPV/EHU grant GIU16/62) to J.l.R.S. and M.B.R.L., and the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement 721236-TREATMENT to M.M

COMPETENCIAS DOCENTES EN LA EDUCACIÓN SUPERIOR: ¿QUÉ INDICA LA LITERATURA?

The new university model inspired by the principles of the European space of higher education entails a change in the roles of teachers, turning them from transmitters into facilitators of learning, while the student assumes the main role of the educational process. This requires incorporating a competency-based approach in higher education, considering that rendering quality education implies that teachers in higher education must have personal, training, research, and pedagogical skills that enable them to perform their teaching function effectively. The aim of this text is to identify relevant pedagogical skills that the teacher should have in order to promote the development of professional and personal values and skills in students, so that they can cope with different situations they will deal with in their future profession and improve their employability. To reach this goal, we conducted a review of the literature at international level, so to design an instrument to identify the most relevant competencies drawn from such review: 1) know and understand; 2) know-how; and 3) knowing how to be.El nuevo modelo de universidad inspirado en los principios del espacio europeo de educación superior ha supuesto un cambio en los roles del profesorado, pasando de transmisor a facilitador de aprendizajes, e incidiendo en el papel del estudiante como protagonista del proceso educativo. Ello supone incorporar el enfoque competencial en la formación universitaria, al considerar que llevar a cabo una enseñanza de calidad implica que el profesorado de enseñanza superior debe tener competencias personales, formativas, de investigación y pedagógicas que le permitan desempeñar su función docente eficazmente. Este trabajo tiene por objetivo la identificación de las competencias pedagógicas más relevantes para favorecer el desarrollo de habilidades, valores y aptitudes profesionales y personales en los estudiantes, con la finalidad de que puedan hacer frente a diferentes situaciones en su labor profesional. Para ello, realizamos una revisión de la literatura a niveles nacional e internacional, a partir de la cual planteamos el diseño de un instrumento en función de las competencias más relevantes: 1) conocer y comprender; 2) saber hacer; y 3) saber ser.O novo modelo de universidade inspirado nos princípios do espaço europeu de ensino superior pressupõe uma mudança nos papéis do professor, que passa de transmissor a facilitador das aprendizagens, e assumindo o estudante o papel protagonista do processo educativo. Isso requer a incorporação de um enfoque nas competências a desenvolver na formação universitária, ao considerar que levar a cabo um ensino de qualidade implica que os professores no ensino superior devam ter competências pessoais, formativas, investigativas e pedagógicas que lhes permitam desempenhar a função docente de modo eficaz. Assim, este texto tem como objetivo a identificação das competências pedagógicas mais relevantes para favorecer o desenvolvimento de habilidades, valores e aptidões profissionais e pessoais nos estudantes, com o objetivo de que estes possam fazer face às diferentes situações que irão enfrentar no seu futuro trabalho profissional e melhorar a sua empregabilidade no mercado laboral. Para isso realizamos uma revisão de literatura em níveis nacional e internacional para poder planear o desenho de um instrumento em função das competências mais relevantes extraídas desta revisão: 1) conhecer e compreender; 2) saber fazer e 3) saber ser

Detection of risk factors that influence weight loss in patients undergoing radiotherapy

AbstractAimTo identify risk factors that influence weight loss in patients receiving radiotherapy.BackgroundIt is a well-known fact that cancer patients can be affected by malnutrition at the onset of the disease and during treatment due to the toxicity. Pretreatment weight loss alone does not predict those who will need nutritional supplementation. Instead, a variety of nutritional and tumor related factors needs to be taken into account.Material and methodsA retrospective study was conducted on 129 patients with different tumor locations. Weight loss was evaluated during radiotherapy and one month after treatment. The impact of age, ECOG, chemotherapy, pretreatment weight loss, tumor location, previous surgery and TNM were analyzed. We aimed to identify a high-risk group of patients before starting treatment.ResultsThe average net weight loss during radiotherapy and one month after treatment for this group of patients was 0.68kg and 1.6kg, respectively. Median weight loss during radiotherapy was 2.6 kg for head and neck (HN) patients and 0.27 kg for other tumor sites (p=0.028). Median weight loss one month after radiotherapy was 3.7kg for HN patients and 1.1kg for the rest of the patients (p=0.034). The median weight loss one month after treatment was 3.2 kg for patients receiving chemotherapy and 0.5kg for those patients who did not receive chemotherapy (p<0.001). A regression analysis determined that HN tumor location and the use of chemotherapy were independent risk factors.ConclusionsNutritional status must be monitored and managed before, during and after treatment. A variety of nutritional and tumor-related factors must be considered. According to our results, head and neck tumors and the use of chemotherapy are the only two factors considered statistically significant. Because patients continue to lose weight after treatment, we recommend close surveillance after radiotherapy

ENO regulates tomato fruit size through the floral meristem development network

A dramatic evolution of fruit size has accompanied the domestication and improvement of fruit-bearing crop species. In tomato (Solanum lycopersicum), naturally occurring cis-regulatory mutations in the genes of the CLAVATA-WUSCHEL signaling pathway have led to a significant increase in fruit size generating enlarged meristems that lead to flowers with extra organs and bigger fruits. In this work, by combining mapping-by-sequencing and CRISPR/Cas9 genome editing methods, we isolated EXCESSIVE NUMBER OF FLORAL ORGANS (ENO), an AP2/ERF transcription factor which regulates floral meristem activity. Thus, the ENO gene mutation gives rise to plants that yield larger multilocular fruits due to an increased size of the floral meristem. Genetic analyses indicate that eno exhibits synergistic effects with mutations at the LOCULE NUMBER (encoding SlWUS) and FASCIATED (encoding SlCLV3) loci, two central players in the evolution of fruit size in the domestication of cultivated tomatoes. Our findings reveal that an eno mutation causes a substantial expansion of SlWUS expression domains in a flower-specific manner. In vitro binding results show that ENO is able to interact with the GGC-box cis-regulatory element within the SlWUS promoter region, suggesting that ENO directly regulates SlWUS expression domains to maintain floral stem-cell homeostasis. Furthermore, the study of natural allelic variation of the ENO locus proved that a cis-regulatory mutation in the promoter of ENO had been targeted by positive selection during the domestication process, setting up the background for significant increases in fruit locule number and fruit size in modern tomatoes

L-Arginine Ameliorates Defective Autophagy in GM2 Gangliosidoses by mTOR Modulation.

AIMS: Tay-Sachs and Sandhoff diseases (GM2 gangliosidosis) are autosomal recessive disorders of lysosomal function that cause progressive neurodegeneration in infants and young children. Impaired hydrolysis catalysed by β-hexosaminidase A (HexA) leads to the accumulation of GM2 ganglioside in neuronal lysosomes. Despite the storage phenotype, the role of autophagy and its regulation by mTOR has yet to be explored in the neuropathogenesis. Accordingly, we investigated the effects on autophagy and lysosomal integrity using skin fibroblasts obtained from patients with Tay-Sachs and Sandhoff diseases. RESULTS: Pathological autophagosomes with impaired autophagic flux, an abnormality confirmed by electron microscopy and biochemical studies revealing the accelerated release of mature cathepsins and HexA into the cytosol, indicating increased lysosomal permeability. GM2 fibroblasts showed diminished mTOR signalling with reduced basal mTOR activity. Accordingly, provision of a positive nutrient signal by L-arginine supplementation partially restored mTOR activity and ameliorated the cytopathological abnormalities. INNOVATION: Our data provide a novel molecular mechanism underlying GM2 gangliosidosis. Impaired autophagy caused by insufficient lysosomal function might represent a new therapeutic target for these diseases. CONCLUSIONS: We contend that the expression of autophagy/lysosome/mTOR-associated molecules may prove useful peripheral biomarkers for facile monitoring of treatment of GM2 gangliosidosis and neurodegenerative disorders that affect the lysosomal function and disrupt autophagy

Selectivity of a thiosemicarbazonatocopper(II) complex towards duplex RNA. Relevant noncovalent interactions both in solid state and solution

Thiosemicarbazones and their metal derivatives have long been screened as antitumor agents, and their interactions with DNA have been analysed. Herein, we describe the synthesis and characterization of compounds containing [CuL]+ entities (HL = pyridine-2-carbaldehyde thiosemicarbazone) and adenine, cytosine or 9-methylguanine, and some of their corresponding nucleotides. For the first time, crystal structures of adenine- and 9-methylguanine-containing thiosemicarbazone complexes are reported. To the best of our knowledge, the first study on the affinity thiosemicarbazone–RNA is also provided here. Experimental and computational studies have shown that [CuL(OH2)]+ entities at low concentration intercalate into dsRNA poly(rA)·poly(rU) through strong hydrogen bonds involving uracil residues and π–π stacking interactions. In fact, noncovalent interactions are present both in the solid state and in solution. This behaviour diverges from that observed with DNA duplexes and creates an optimistic outlook in achieving selective binding to RNA for subsequent possible medical applications.Obra Social “la Caixa” (OSLC-2012-007), Ministerio de Economía y Competitividad and FEDER funds (CTQ2013-48937-C2-1-P, CTQ2015-70371- REDT, MAT2012-34740 and CTQ2014-58812-C2-2-R), Junta de Castilla y León (BU237U13), the Basque Government (IT-779- 13), Gerencia Regional de Salud, Consejería de Sanidad, Junta de Castilla y León (GRS 1023/A/14 and GR172)