Articulação entre a vida profissional, familiar e pessoal: Um estudo de caso na PSP do COMETLIS

Dissertação de Mestrado em Sociologia das Organizações e do TrabalhoO risco e stress da profissão de polícia desencadeiam um desgaste físico e psicológico, que coloca desafios consideráveis à articulação entre a vida profissional, familiar e pessoal. O presente estudo tem como objectivo geral conhecer as condições familiares e profissionais que influenciam a articulação entre a vida profissional, familiar e pessoal dos/as Agentes da Polícia de Segurança Pública (PSP). A nível metodológico foi aplicado um inquérito por questionário a 414 Agentes de 11 Divisões do Comando Metropolitano de Polícia de Lisboa (COMETLIS). No plano empírico testaram-se os modelos de Allen (2001), Carlson, Kacmar e Williams (2000) e Neto (2014), com vista a avaliar a articulação entre a vida profissional, familiar e pessoal dos/as Agentes da PSP do COMETLIS. Os resultados apontam para a influência de ter filhos/as menores de idade, ter idosos/as ou pessoas dependentes a seu cargo, assim como a idade como variáveis com influência considerável na articulação com a vida profissional. A nível profissional, as variáveis, trabalho por turnos, categoria profissional, anos de serviço e estar deslocado/a em serviço, foram apontadas pelos/as Agentes como condicionantes na articulação com a vida familiar e pessoal. Neste estudo verificam-se ainda diferenças de género na articulação entre a vida profissional, familiar e pessoal.The danger and stress of police officer career induce physical and psychological distress, which poses considerable challenges articulating professional and familiar/personal life. The present study aimed to know the family and professional conditions that influence the articulation between personal, family and professional lives of the Public Security Police (PSP) police agents. At the methodological level, a questionnaire survey was applied to 414 police agents of 11 divisions of Lisbon Metropolitan Police Command (COMETLIS). At the empirical level, the models of Allen (2001), Carlson, Kacmar and Williams (2000) and Neto (2014) were tested in order to evaluate the articulation between professional and familiar/personal life of police agents. The results point to the influence of having underage children, having elderly or dependent people, as well as age as variables with considerable influence on the articulation with professional life. At the professional level, the variables, shift work, professional category, years of service and being displaced in service, were pointed out by the Agents as conditioning factors in the articulation with family and personal life. In this study there are also gender differences in the articulation between professional, family and personal life.N/

Validation of a peptide nucleic acid fluorescence in situ hybridization for the specific detection of salmonella species in food matrices

Salmonella is a Gram-negative flagellated rod-shaped bacterium that is one of the most important etiological agents in bacterial foodborne diseases [1,2]. Despite human salmonellosis generally presenting as a self-limiting episode of enterocolitis, the infection can degenerate into chronic and debilitating conditions [2]. To diagnose a Salmonella infection, standard cultural methods are routinely used, which implies bacterial identification by biochemical and serological tests, to confirm the suspect colonies grown on the selective agar [3]. However, this methodology is time-consuming and takes too long to deliver the results [4]. Due to these limitations, more rapid techniques for detection have been developed [5-7]. For that, in this study, we developed a novel Peptide Nucleic Acid Fluorescence in situ Hybridization (PNA FISH) method for the specific detection of Salmonella spp. The method was based on a new PNA probe, SalPNA1692, coupled with a novel blocker probe in a 1:1 ratio. The method was optimized for the detection of Salmonella in food samples through an evaluation of several rich and selective enrichment broths. The best outcome was achieved using Buffered Peptone water as a pre-enrichment for 24 h followed by 16 h of selective enrichment in RambaQuick broth. For validation in food samples, fresh ground beef was artificially contaminated with two ranges of inoculum: a low level (0.22 CFU/25 g) and a high level (210 CFU/25 g). For both levels of contamination, the confirmed positives were the same comparing the PNA-FISH method and the reference method (ISO 6579-1: 2017). The new PNA-FISH method presented a specificity of 100 % and is a faster time-to-result method, making it a good candidate for routine application in food safety laboratories.info:eu-repo/semantics/publishedVersio

SARS-CoV-2 introductions and early dynamics of the epidemic in Portugal

Genomic surveillance of SARS-CoV-2 in Portugal was rapidly implemented by the National Institute of Health in the early stages of the COVID-19 epidemic, in collaboration with more than 50 laboratories distributed nationwide. Methods By applying recent phylodynamic models that allow integration of individual-based travel history, we reconstructed and characterized the spatio-temporal dynamics of SARSCoV-2 introductions and early dissemination in Portugal. Results We detected at least 277 independent SARS-CoV-2 introductions, mostly from European countries (namely the United Kingdom, Spain, France, Italy, and Switzerland), which were consistent with the countries with the highest connectivity with Portugal. Although most introductions were estimated to have occurred during early March 2020, it is likely that SARS-CoV-2 was silently circulating in Portugal throughout February, before the first cases were confirmed. Conclusions Here we conclude that the earlier implementation of measures could have minimized the number of introductions and subsequent virus expansion in Portugal. This study lays the foundation for genomic epidemiology of SARS-CoV-2 in Portugal, and highlights the need for systematic and geographically-representative genomic surveillance.We gratefully acknowledge to Sara Hill and Nuno Faria (University of Oxford) and Joshua Quick and Nick Loman (University of Birmingham) for kindly providing us with the initial sets of Artic Network primers for NGS; Rafael Mamede (MRamirez team, IMM, Lisbon) for developing and sharing a bioinformatics script for sequence curation (https://github.com/rfm-targa/BioinfUtils); Philippe Lemey (KU Leuven) for providing guidance on the implementation of the phylodynamic models; Joshua L. Cherry (National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health) for providing guidance with the subsampling strategies; and all authors, originating and submitting laboratories who have contributed genome data on GISAID (https://www.gisaid.org/) on which part of this research is based. The opinions expressed in this article are those of the authors and do not reflect the view of the National Institutes of Health, the Department of Health and Human Services, or the United States government. This study is co-funded by Fundação para a Ciência e Tecnologia and Agência de Investigação Clínica e Inovação Biomédica (234_596874175) on behalf of the Research 4 COVID-19 call. Some infrastructural resources used in this study come from the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT).info:eu-repo/semantics/publishedVersio

MAMMALS IN PORTUGAL : A data set of terrestrial, volant, and marine mammal occurrences in P ortugal

Mammals are threatened worldwide, with 26% of all species being includedin the IUCN threatened categories. This overall pattern is primarily associatedwith habitat loss or degradation, and human persecution for terrestrial mam-mals, and pollution, open net fishing, climate change, and prey depletion formarine mammals. Mammals play a key role in maintaining ecosystems func-tionality and resilience, and therefore information on their distribution is cru-cial to delineate and support conservation actions. MAMMALS INPORTUGAL is a publicly available data set compiling unpublishedgeoreferenced occurrence records of 92 terrestrial, volant, and marine mam-mals in mainland Portugal and archipelagos of the Azores and Madeira thatincludes 105,026 data entries between 1873 and 2021 (72% of the data occur-ring in 2000 and 2021). The methods used to collect the data were: live obser-vations/captures (43%), sign surveys (35%), camera trapping (16%),bioacoustics surveys (4%) and radiotracking, and inquiries that represent lessthan 1% of the records. The data set includes 13 types of records: (1) burrowsjsoil moundsjtunnel, (2) capture, (3) colony, (4) dead animaljhairjskullsjjaws, (5) genetic confirmation, (6) inquiries, (7) observation of live animal (8),observation in shelters, (9) photo trappingjvideo, (10) predators dietjpelletsjpine cones/nuts, (11) scatjtrackjditch, (12) telemetry and (13) vocalizationjecholocation. The spatial uncertainty of most records ranges between 0 and100 m (76%). Rodentia (n=31,573) has the highest number of records followedby Chiroptera (n=18,857), Carnivora (n=18,594), Lagomorpha (n=17,496),Cetartiodactyla (n=11,568) and Eulipotyphla (n=7008). The data setincludes records of species classified by the IUCN as threatened(e.g.,Oryctolagus cuniculus[n=12,159],Monachus monachus[n=1,512],andLynx pardinus[n=197]). We believe that this data set may stimulate thepublication of other European countries data sets that would certainly contrib-ute to ecology and conservation-related research, and therefore assisting onthe development of more accurate and tailored conservation managementstrategies for each species. There are no copyright restrictions; please cite thisdata paper when the data are used in publications.info:eu-repo/semantics/publishedVersio

Mammals in Portugal: a data set of terrestrial, volant, and marine mammal occurrences in Portugal

Mammals are threatened worldwide, with ~26% of all species being included in the IUCN threatened categories. This overall pattern is primarily associated with habitat loss or degradation, and human persecution for terrestrial mammals, and pollution, open net fishing, climate change, and prey depletion for marine mammals. Mammals play a key role in maintaining ecosystems functionality and resilience, and therefore information on their distribution is crucial to delineate and support conservation actions. MAMMALS IN PORTUGAL is a publicly available data set compiling unpublished georeferenced occurrence records of 92 terrestrial, volant, and marine mammals in mainland Portugal and archipelagos of the Azores and Madeira that includes 105,026 data entries between 1873 and 2021 (72% of the data occurring in 2000 and 2021). The methods used to collect the data were: live observations/captures (43%), sign surveys (35%), camera trapping (16%), bioacoustics surveys (4%) and radiotracking, and inquiries that represent less than 1% of the records. The data set includes 13 types of records: (1) burrows | soil mounds | tunnel, (2) capture, (3) colony, (4) dead animal | hair | skulls | jaws, (5) genetic confirmation, (6) inquiries, (7) observation of live animal (8), observation in shelters, (9) photo trapping | video, (10) predators diet | pellets | pine cones/nuts, (11) scat | track | ditch, (12) telemetry and (13) vocalization | echolocation. The spatial uncertainty of most records ranges between 0 and 100 m (76%). Rodentia (n =31,573) has the highest number of records followed by Chiroptera (n = 18,857), Carnivora (n = 18,594), Lagomorpha (n = 17,496), Cetartiodactyla (n = 11,568) and Eulipotyphla (n = 7008). The data set includes records of species classified by the IUCN as threatened (e.g., Oryctolagus cuniculus [n = 12,159], Monachus monachus [n = 1,512], and Lynx pardinus [n = 197]). We believe that this data set may stimulate the publication of other European countries data sets that would certainly contribute to ecology and conservation-related research, and therefore assisting on the development of more accurate and tailored conservation management strategies for each species. There are no copyright restrictions; please cite this data paper when the data are used in publications

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Ratos tratados com dieta cetogênica apresentam aumento de tecido adiposo mediado pela elevação da atividade da fosfoenolpiruvato carboxicinase

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Ratos tratados com dieta cetogênica apresentam aumento de tecido adiposo mediado pela elevação da atividade da fosfoenolpiruvato carboxicinase

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Resumos em andamento - Educação

Resumos em andamento - Educaçã

Resumos em andamento - Educação

Resumos em andamento - Educaçã