Eye Tracking Analysis in Reading Online Newspapers

Reading online newspapers is increasingly becoming more common, so that thousands of newspapers are published online today. Despite this development, there are many unanswered questions concerning subjects’ behaviour during reading an online newspapers. Recording eye movements when a subject is navigating within a news website can provide quantitative and objective information on subject’s behaviour and combined with other methodologies – usability testing, focus groups, log analysis - represents a powerful tool for improving news websites functionality, and by consequence their achievement among readers. Two Italian newspaper websites have been considered for the study described in this paper and the analysis has been focused on the exploration behaviour within the newspaper home page and the reading of article pages

Detecting expert’s eye using a multiple-kernel Relevance Vector Machine

Decoding mental states from the pattern of neural activity or overt behavior is an intensely pursued goal. Here we applied machine learning to detect expertise from the oculomotor behavior of novice and expert billiard players during free viewing of a filmed billiard match with no specific task, and in a dynamic trajectory prediction task involving ad-hoc, occluded billiard shots. We have adopted a ground framework for feature space fusion and a Bayesian sparse classifier, namely, a Relevance Vector Machine. By testing different combinations of simple oculomotor features (gaze shifts amplitude and direction, and fixation duration), we could classify on an individual basis which group - novice or expert - the observers belonged to with an accuracy of 82% and 87%, respectively for the match and the shots. These results provide evidence that, at least in the particular domain of billiard sport, a signature of expertise is hidden in very basic aspects of oculomotor behavior, and that expertise can be detected at the individual level both with ad-hoc testing conditions and under naturalistic conditions - and suitable data mining. Our procedure paves the way for the development of a test for the “expert’s eye”, and promotes the use of eye movements as an additional signal source in Brain-Computer-Interface (BCI) systems

Development and Validation of MPS-Based System for Human Appearance Prediction in Challenging Forensic Samples

Forensic DNA phenotyping (FDP) provides the ability to predict the human external traits from unknown sample donors, directly from minute amounts of DNA found at the crime scene. We developed a MPS multiplex assay, with the aim of genotyping all 41 DNA markers included in the HIrisPlex-S system for simultaneous prediction of eye, hair and skin colours. Forensic samples such as blood, skeletal remains, touch DNA, saliva swab, artificially degraded samples together with individuals with known phenotypes and a set of 2800 M control DNA were sequenced on the Ion Torrent platform in order to evaluate the concordance testing results and the forensic suitability of the 41-plex MPS assay. The panel was evaluated by testing a different number of PCR cycles and the volume of reagents for library preparation. The study demonstrated that full and reliable profiles were obtained with 0.1–5 ng, even with high degraded DNA. The increment of the number of PCR cycles results in an improvement of correctly genotyping and phenotyping for samples with low amounts of degraded DNA but higher frequencies of artefacts were found. The high DNA degradation level did not influence the correct genotyping and phenotyping and the critical parameter affecting the result is the quantity of input DNA. Eye and hair colour was predicted in 92.60% of individuals and skin colour in 85.15% of individuals. The results suggest that this MPS assay is robust, highly sensitive and useful for human pigmentation prediction in the forensic genetic field

A global analysis of Y-chromosomal haplotype diversity for 23 STR loci

In a worldwide collaborative effort, 19,630 Y-chromosomes were sampled from 129 different populations in 51 countries. These chromosomes were typed for 23 short-tandem repeat (STR) loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, GATAH4, DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643) and using the PowerPlex Y23 System (PPY23, Promega Corporation, Madison, WI). Locus-specific allelic spectra of these markers were determined and a consistently high level of allelic diversity was observed. A considerable number of null, duplicate and off-ladder alleles were revealed. Standard single-locus and haplotype-based parameters were calculated and compared between subsets of Y-STR markers established for forensic casework. The PPY23 marker set provides substantially stronger discriminatory power than other available kits but at the same time reveals the same general patterns of population structure as other marker sets. A strong correlation was observed between the number of Y-STRs included in a marker set and some of the forensic parameters under study. Interestingly a weak but consistent trend toward smaller genetic distances resulting from larger numbers of markers became apparent.Peer reviewe