12 research outputs found
Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System
The paper presents the development of a “proof-of-principle” hands-free and self-contained diagnostic platform for detection of human papillomavirus (HPV) E6/E7 mRNA in clinical specimens. The automated platform performs chip-based sample preconcentration, nucleic acid extraction, amplification, and real-time fluorescent detection with minimal user interfacing. It consists of two modular prototypes, one for sample preparation and one for amplification and detection; however, a common interface is available to facilitate later integration into one single module. Nucleic acid extracts (n = 28) from cervical cytology specimens extracted on the sample preparation chip were tested using the PreTect HPV-Proofer and achieved an overall detection rate for HPV across all dilutions of 50%–85.7%. A subset of 6 clinical samples extracted on the sample preparation chip module was chosen for complete validation on the NASBA chip module. For 4 of the samples, a 100% amplification for HPV 16 or 33 was obtained at the 1 : 10 dilution for microfluidic channels that filled correctly. The modules of a “sample-in, answer-out” diagnostic platform have been demonstrated from clinical sample input through sample preparation, amplification and final detection
Enhanced Liquid Metal Micro Droplet Generation by Pneumatic Actuation Based on the StarJet Method
We present a novel pneumatic actuation system for generation of liquid metal droplets according to the so-called StarJet method. In contrast to our previous work, the performance of the device has been significantly improved: the maximum droplet generation frequency in continuous mode has been increased to fmax = 11 kHz (formerly fmax = 4 kHz). In addition, the droplet diameter has been reduced to 60 μm. Therefore, a new fabrication process for the silicon nozzle chips has been developed enabling the production of smaller nozzle chips with higher surface quality. The size of the metal reservoir has been increased to hold up to 22 mL liquid metal and the performance and durability of the actuator has been improved by using stainless steel and a second pneumatic connection to control the sheath flow. Experimental results are presented regarding the characterization of the droplet generation, as well as printed metal structures
Completely Superhydrophobic PDMS Surfaces for Microfluidics
This study presents a straightforward two-step fabrication
process of durable, completely superhydrophobic microchannels in PDMS.
First, a composite material of PDMS/PTFE particles is prepared and
used to replicate a master microstructure. Superhydrophobic surfaces
are formed by subsequent plasma treatment, in which the PDMS is isotropically
etched and PTFE particles are excavated. We compare the advancing
and receding contact angles of intrinsic PDMS samples and composite
PTFE/PDMS samples (1 wt %, 8 wt %, and 15 wt % PTFE particle concentration)
and demonstrate that both the horizontal and vertical surfaces are
indeed superhydrophobic. The best superhydrophobicity is observed
for samples with a PTFE particle concentration of 15 wt %, which have
advancing and receding contact angles of 159° ± 4°
and 158° ± 3°, respectively