A real-time Malaysian automatic license plate recognition (M-ALPR) using hybrid fuzzy

The Road Transport Department of Malaysia has endorsed a specification for car plates that includes the font and size of characters that must be followed by car owners. However, there are cases where this specification is not followed. This paper proposes a new methodology to segment and recognize Malaysian car license plates automatically. The proposed methodology solves the problem of segmenting different length licenses such as license with different number of character and number. There are two main objectives for this paper: first is to develop fuzzy rules to recognize the segmented characters and numbers from the same input-sets, which is the same size without overlapping between the characters and numbers sets. Secondly, this paper proposes a method to recognize non-standard plates by Template Matching theorem. Finally, the hybrid method of Fuzzy and Template matching is tested on 300 samples of car images captured in outdoor environment. The results yield 90.4% recognition accuracy, the Fuzzy based required 1.7 seconds and Template matching based took 0.75 seconds to perform the recognition. The adaptability factor of the hybrid method is also discussed

An improved Malaysian automatic license plate recognition (M-ALPR) system using hybrid fuzzy in C++ environment

In this paper, an improved hybrid fuzzy technique (Fuzzy Logic and Template matching) for Malaysian Automatic License Plate Recognition (M-ALPR) system is proposed. The system is proposed to reduce the program complexity of the existing M-ALPR system and to decrease the processing time of recognizing Malaysian license plates. First, the algorithm to recognize the license plates is presented, by taking advantage of Matlab and C++ programming language benefits in order to increase system efficiency. Feature extraction using vertical line counter is introduced in this system. Later, with the help of OpenCV, the hybrid fuzzy technique is developed using the C++ language. Then, the comparison between these two implementations on M-ALPR system was reported. The improved system was tested on 740 samples images from real scene and the results show that the proposed improvement supports the accurateness and high speed processing of M-ALPR system

Spiral Microchannels with Trapezoidal Cross Section Fabricated by Femtosecond Laser Ablation in Glass for the Inertial Separation of Microparticles

The fabrication and testing of spiral microchannels with a trapezoidal cross section for the passive separation of microparticles is reported in this article. In contrast to previously reported fabrication methods, the fabrication of trapezoidal spiral channels in glass substrates using a femtosecond laser is reported for the first time in this paper. Femtosecond laser ablation has been proposed as an accurate and fast prototyping method with the ability to create 3D features such as slanted-base channels. Moreover, the fabrication in borosilicate glass substrates can provide high optical transparency, thermal resistance, dimensional stability, and chemical inertness. Post-processing steps of the laser engraved glass substrate are also detailed in this paper including hydrogen fluoride (HF) dipping, chemical cleaning, surface activation, and thermal bonding. Optical 3D images of the fabricated chips confirmed a good fabrication accuracy and acceptable surface roughness. To evaluate the particle separation function of the microfluidic chip, 5 μm, 10 μm, and 15 μm particles were focused and recovered from the two outlets of the spiral channel. In conclusion, the new chemically inert separation chip can be utilized in biological or chemical processes where different sizes of cells or particles must be separated, i.e., red blood cells, circulating tumor cells, and technical particle suspensions

Event-triggered logical flow control for comprehensive process integration of multi-step assays on centrifugal microfluidic platforms

Content in the UH Research Archive is made available for personal research, educational, and non-commercial purposes only. Unless otherwise stated, all content is protected by copyright, and in the absence of an open license, permissions for further re-use should be sought from the publisher, the author, or other copyright holder.The centrifugal "lab-on-a-disc" concept has proven to have great potential for process integration of bioanalytical assays, in particular where ease-of-use, ruggedness, portability, fast turn-around time and cost efficiency are of paramount importance. Yet, as all liquids residing on the disc are exposed to the same centrifugal field, an inherent challenge of these systems remains the automation of multi-step, multi-liquid sample processing and subsequent detection. In order to orchestrate the underlying bioanalytical protocols, an ample palette of rotationally and externally actuated valving schemes has been developed. While excelling with the level of flow control, externally actuated valves require interaction with peripheral instrumentation, thus compromising the conceptual simplicity of the centrifugal platform. In turn, for rotationally controlled schemes, such as common capillary burst valves, typical manufacturing tolerances tend to limit the number of consecutive laboratory unit operations (LUOs) that can be automated on a single disc. In this paper, a major advancement on recently established dissolvable film (DF) valving is presented; for the very first time, a liquid handling sequence can be controlled in response to completion of preceding liquid transfer event, i.e. completely independent of external stimulus or changes in speed of disc rotation. The basic, event-triggered valve configuration is further adapted to leverage conditional, large-scale process integration. First, we demonstrate a fluidic network on a disc encompassing 10 discrete valving steps including logical relationships such as an AND-conditional as well as serial and parallel flow control. Then we present a disc which is capable of implementing common laboratory unit operations such as metering and selective routing of flows. Finally, as a pilot study, these functions are integrated on a single disc to automate a common, multi-step lab protocol for the extraction of total RNA from mammalian cell homogenate.Peer reviewe

Paper imbibition for timing of multi-step liquid handling protocols on event-triggered centrifugal microfluidic lab-on-a-disc platforms

This document is the Accepted Manuscript version of the following article: David J. Kinahan, Sinéad M. Kearney, Olivier P. Faneuil, Macdara T. Glynn, Nikolay Dimov, and Jens Ducrée, ‘Paper imbibition for timing of multi-step liquid handling protocols on event-triggered centrifugal microfluidic lab-on-a-disc platforms’, RSC Advances, Vol. 5 (3): 1818-1826, 2015, doi: https://doi.org/10.1039/C4RA14887H, published by the Royal Society of Chemistry.Rotational microfluidic platforms have attracted swiftly growing interest over the last decade due to their suitability for integration and automation of sample preparation and detection. Valving is of pivotal importance on these compact "Lab-on-a-Disc" (LoaD) platforms as all liquids are exposed to the same centrifugal field. A number of valving technologies have been developed to coordinate timing of serial and/or parallel multi-step/multi-liquid assay protocols comprising of laboratory unit operations (LUOs) such as the release, metering and mixing of sample and reagents. So far these valving techniques could be broadly categorised into rotationally controlled or externally actuated schemes. Only recently a new, "event-triggered" flow control has been introduced. In this approach, a valve is opened upon arrival of a liquid at a defined destination on the disc; this innovative mechanism for the first time permits the cascading of LUOs independent of the spin rate. In one technology, dissolvable films (DFs) are configured with a pneumatic chamber to offer function akin to an electrical relay. Dissolving one DF, termed the control film (CF), results in the release of liquid at a distal location through a so-called load film (LF). In this paper, a new method for temporal control of actuating DF-based, event-triggered CFs which are serially aligned at defined distances along a paper strip is introduced. Liquids are transported through the paper strip at a given velocity, thus setting well-defined intervals between subsequent LUOs, e.g. incubation steps. As a proof-of-concept, we present a disc with integrated metering and mixing which can perform a prototypical, 4-fold serial dilution; a common function in bioanalytical protocols. Imbibition of the paper strip sequentially opens five valves for serial dilution and mixing. To illustrate an unprecedented level of on-disc automation, this is followed by a branched cascade of 17 event-triggered valves (for a total of 22 liquid handling steps) which completes the serial dilution protocol.Peer reviewedFinal Accepted Versio

Vacuum/Compression Valving (VCV) Using Parrafin-Wax on a Centrifugal Microfluidic CD Platform

This paper introduces novel vacuum/compression valves (VCVs) utilizing paraffin wax. A VCV is implemented by sealing the venting channel/hole with wax plugs (for normally-closed valve), or to be sealed by wax (for normally-open valve), and is activated by localized heating on the CD surface. We demonstrate that the VCV provides the advantages of avoiding unnecessary heating of the sample/reagents in the diagnostic process, allowing for vacuum sealing of the CD, and clear separation of the paraffin wax from the sample/reagents in the microfluidic process. As a proof of concept, the microfluidic processes of liquid flow switching and liquid metering is demonstrated with the VCV. Results show that the VCV lowers the required spinning frequency to perform the microfluidic processes with high accuracy and ease of control.open5

Development of a Passive Liquid Valve (PLV) Utilizing a Pressure Equilibrium Phenomenon on the Centrifugal Microfluidic Platform

In this paper, we propose an easy-to-implement passive liquid valve (PLV) for the microfluidic compact-disc (CD). This valve can be implemented by introducing venting chambers to control the air flow of the source and destination chambers. The PLV mechanism is based on equalizing the main forces acting on the microfluidic CD (i.e., the centrifugal and capillary forces) to control the burst frequency of the source chamber liquid. For a better understanding of the physics behind the proposed PLV, an analytical model is described. Moreover, three parameters that control the effectiveness of the proposed valve, i.e., the liquid height, liquid density, and venting chamber position with respect to the CD center, are tested experimentally. To demonstrate the ability of the proposed PLV valve, microfluidic liquid switching and liquid metering are performed. In addition, a Bradford assay is performed to measure the protein concentration and evaluated in comparison to the benchtop procedure. The result shows that the proposed valve can be implemented in any microfluidic process that requires simplicity and accuracy. Moreover, the developed valve increases the flexibility of the centrifugal CD platform for passive control of the liquid flow without the need for an external force or trigger

Real-time Malaysian automatic license plate recognition using hybrid fuzzy logic with skew detection and correction method

Automatic License Plate Recognition (ALPR) system is a mass surveillance method that uses optical character recognition on images to read the license plates on vehicles. This system has been used widely overseas. However, the different forms of Malaysian license plates still a problem that makes this system harder to be applied locally. The proposed license plate recognition algorithm is aimed to recognize the different Malaysian license plates by employing two methods: Fuzzy Logic to recognize standard license plate (the plates which consist of characters and numbers), and Template Matching to recognize non-standard plates (the plates which consist of non-standard word and numbers). Mathematical Morphology is the first preprocessing step used to enhance Malaysian license plate image quality, by removing noise from the binarized image. The second step is to remove license plate borders by implementing Mathematical Morphology process with conditional statements. The third preprocessing step is a new Skew Detection and Correction (SDC) method proposed to correct the skewness of license plate image. License plate level testing follows the preprocessing step in order to check if the license plate is one or two rows (the license plate elements are in one or two rows). The standard and non-standard test is performed by checking if the input image is representing a standard or a non-standard plate. Vertical scanning (VS) and horizontal scanning (HS) have been used to segment license plate image elements. Segmentation process is the step where license plate elements are segmented. The next step is to forward the extracted characters and numbers to the Fuzzy Logic system to be recognized in case of standard license plates input, while forward nonstandard words images to the Template Matching in order to be recognized in case of non-standard license plates input. The output of recognition step will be a string of numbers and characters which represent the recognized license plate. The proposed M-LPR algorithm has shown an impressive result to recognize different Malaysian license plate forms. Fuzzy Logic system has been tested on standard license plate shows 92.16% recognition accuracy and 0.88 second processing time. The Template Matching shows 92% recognition accuracy and 1.06 second processing time when it is tested on non-standard license plate. The proposed SDC method has been evaluated by comparing with different other existing SDC methods such as Hough Transform, Projection Profile, Mathematical Morphology and Bounding Box methods