3,103 research outputs found
Development methodology to share vehicles optimizing the variability of the mileage
A simulation is a tool used to visualize the behaviors of a system, which will later help make decisions regarding how to handle the variables involved in the system, as well as the specific changes that have to be made. This study shows a case of vehicle allocation for different people within a company, evaluating methodologies, vehicle rotation to reduce the variance of the mileage and eliminating penalties with rental agencies for exceeding the permitted mileage. The paper shows a literature review of allocation models and similar studies, and later displays a detailed description of the problem, the variables that was used, the composition of the simulation and the optimization model that were generated, the results of the simulation, and finally, the findings of the research
Methylation alterations are not a major cause of PTTG1 missregulation
Background: On its physiological cellular context, PTTG1 controls sister chromatid segregation
during mitosis. Within its crosstalk to the cellular arrest machinery, relies a checkpoint of integrity
for which gained the over name of securin. PTTG1 was found to promote malignant transformation
in 3T3 fibroblasts, and further found to be overexpressed in different tumor types. More recently,
PTTG1 has been also related to different processes such as DNA repair and found to trans-activate
different cellular pathways involving c-myc, bax or p53, among others. PTTG1 over-expression has
been correlated to a worse prognosis in thyroid, lung, colorectal cancer patients, and it can not be
excluded that this effect may also occur in other tumor types. Despite the clinical relevance and
the increasing molecular characterization of PTTG1, the reason for its up-regulation remains
unclear.
Method: We analysed PTTG1 differential expression in PC-3, DU-145 and LNCaP tumor cell
lines, cultured in the presence of the methyl-transferase inhibitor 5-Aza-2'-deoxycytidine. We also
tested whether the CpG island mapping PTTG1 proximal promoter evidenced a differential
methylation pattern in differentiated thyroid cancer biopsies concordant to their PTTG1
immunohistochemistry status. Finally, we performed whole-genome LOH studies using Affymetix
50 K microarray technology and FRET analysis to search for allelic imbalances comprising the
PTTG1 locus.
Conclusion: Our data suggest that neither methylation alterations nor LOH are involved in
PTTG1 over-expression. These data, together with those previously reported, point towards a
post-transcriptional level of missregulation associated to PTTG1 over-expression.This project was funded by The Fundación de Investigación Biomédica Mutua Madrileña Automovilista. Neocodex have been partially funded by the Ministerio de Educación y Ciencia of Spain (FIT-010000-2004-69, PTQ04-1-0006, PTQ2003-0549, PTQ2003-0546 and PTQ2003-0783). MAJ was also supported by SAF2005- 07713-C03-03 and CS by FIS 06/757
Procesos fisicoquímicos asociados con isquemia en miocardio y su influencia en arritmias: un estudio de simulación
Los fuertes cambios fisicoquímicos sufridos por el miocardio en eventos isquémicos componen el sustrato funcional para la formación de reentradas de potencial eléctrico en superficie. Estas alteraciones son modeladas en mallas bidimensionales, utilizando modelo biofísico de Luo-Rudy en tejido epicárdico. Se obtienen reentradas en forma de ocho que representa un desbalance eléctrico de tipo fuente-sumidero. Se cree que este desbalance es clave en fibrilación ventricular
Procesos fisicoquímicos asociados con isquemia en miocardio y su influencia en arritmias: un estudio de simulación
Los fuertes cambios fisicoquímicos sufridos por el miocardio en eventos isquémicos componen el sustrato funcional para la formación de reentradas de potencial eléctrico en superficie. Estas alteraciones son modeladas en mallas bidimensionales, utilizando modelo biofísico de Luo-Rudy en tejido epicárdico. Se obtienen reentradas en forma de ocho que representa un desbalance eléctrico de tipo fuente-sumidero. Se cree que este desbalance es clave en fibrilación ventricular
ALMA observations of Elias 2–24: a protoplanetary disk with multiple gaps in the Ophiuchus molecular cloud
We present ALMA 1.3 mm continuum observations at 0. 2 (25 au) resolution of Elias 2–24, one of the largest and brightest protoplanetary disks in the Ophiuchus Molecular Cloud, and we report the presence of three partially resolved concentric gaps located at ∼20, 52, and 87 au from the star. We perform radiative transfer modeling of the disk to constrain its surface density and temperature radial profile and place the disk structure in the context of mechanisms capable of forming narrow gaps such as condensation fronts and dynamical clearing by actively forming planets. In particular, we estimate the disk temperature at the locations of the gaps to be 23, 15, and 12 K (at 20, 52, and 87 au, respectively), very close to the expected snowlines of CO (23–28 K) and N2 (12–15 K). Similarly, by assuming that the widths of the gaps correspond to 4–8× the Hill radii of forming planets (as suggested by numerical simulations), we estimate planet masses in the range of 0.2 1.5 – MJup, 1.0 8.0 – MJup, and 0.02 0.15 – MJup for the inner, middle, and outer gap, respectively. Given the surface density profile of the disk, the amount of “missing mass” at the location of each one of these gaps (between 4 and 20 MJup) is more than sufficient to account for the formation of such planets.Fil: Cieza, Lucas A.. Universidad Diego Portales; ChileFil: Casassus, Simon. Universidad de Chile; ChileFil: Pérez, Sebastian. Universidad de Chile; ChileFil: Hales, Antonio. Alma Observatory; ChileFil: Cárcamo, Miguel. Universidad de Chile; ChileFil: Ansdell, Megan. University of California at Berkeley; Estados UnidosFil: Avenhaus, Henning. Universitat Zurich; SuizaFil: Bayo, Amelia. Universidad de Valparaiso; ChileFil: Bertrang, Gesa H.-M.. Universidad Diego Portales; ChileFil: Cánovas, Hector. Agencia Espacial Europea; EspañaFil: Christiaens, Valentin. Universidad de Chile; ChileFil: Dent, William. Alma Observatory; ChileFil: Ferrero, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Gamen, Roberto Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Olofsson, Johan. Universidad de Valparaiso; ChileFil: Orcajo, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Osses, Axel. Universidad de Chile; ChileFil: Peña Ramirez, Karla. Universidad de Antofagasta; ChileFil: Principe, David. Massachusetts Institute of Technology; Estados UnidosFil: Ruíz Rodríguez, Dary. Rochester Institute Of Technology; Estados UnidosFil: Schreiber, Matthias R.. Universidad de Valparaiso; ChileFil: Plas, Gerrit van der. Univ. Grenoble Alpes; SuizaFil: Williams, Jonathan P.. Institute For Astronomy, University Of Hawaii; Estados UnidosFil: Zurlo, Alice. Universidad Diego Portales; Chil
Freezing and large time scales induced by geometrical frustration
We investigate the properties of an effective Hamiltonian with competing
interactions involving spin and chirality variables, relevant for the
description of the {\it trimerized} version of the spin-1/2 {\it kagome}
antiferromagnet. Using classical Monte Carlo simulations, we show that
remarkable behaviors develop at very low temperatures. Through an {\it order by
disorder} mechanism, the low-energy states are characterized by a dynamical
freezing of the chiralities, which decouples the lattice into ``dimers'' and
``triangles'' of antiferromagnetically coupled spins. Under the presence of an
external magnetic field, the particular topology of the chiralities induces a
very slow spin dynamics, reminiscent of what happens in ordinary spin glasses.Comment: 12 pages, 13 figure
In silico ischaemia-induced reentry at the Purkinjeventricle interface
This computational modelling work illustrates the influence of hyperkalaemia and electrical uncoupling induced by defined ischaemia on action potential (AP) propagation and the incidence of reentry at the Purkinjeventricle interface in mammalian hearts.
Unidimensional and bidimensional models of the Purkinjeventricle subsystem, including ischaemic conditions (defined as phase 1B) in the ventricle and an ischaemic border zone, were developed by altering several important electrophysiological parameters of the LuoRudy AP model of the ventricular myocyte. Purkinje electrical activity was modelled using the equations of DiFrancesco and Noble. Our study suggests that an extracellular potassium concentration [K](o) 14 mM and a slight decrease in intercellular coupling induced by ischaemia in ventricle can cause conduction block from Purkinje to ventricle. Under these conditions, propagation from ventricle to Purkinje is possible. Thus, unidirectional block (UDB) and reentry can result. When conditions of UDB are met, retrograde propagation with a long delay (320 ms) may re-excite Purkinje cells, and give rise to a reentrant pathway. This induced reentry may be the origin of arrhythmias observed in phase 1B ischaemia.
In a defined setting of ischaemia (phase 1B), a small amount of uncoupling between ventricular cells, as well as between Purkinje and ventricular tissue, may induce UDBs and reentry. Hyperkalaemia is also confirmed to be an important factor in the genesis of reentrant rhythms, since it regulates the range of coupling in which UDBs may be induced.This work was supported: (i) by the European Commission preDiCT grant (DG-INFSO-224381), (ii) by the 'VI Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica' from the Ministerio de Economia y Competitividad of Spain (grant number TIN2012-37546-C03-01) and the European Commission (European Regional Development Funds - ERDF - FEDER), and (iii) by the Programa de Apoyo a la Investigacioon y Desarrollo (PAID-06-11-2002) de la Universidad Politecnica de Valencia, Programa Prometeo (PROMETEO/2012/030) de la Conselleria d'Educacio Formacio I Ocupacio, Generalitat Valenciana, and (iv) Direccion General de Politica Cientifica de la Generalitat Valenciana (GV/2013/119).Esteban Ramírez, J.; Saiz Rodríguez, FJ.; Romero Pérez, L.; Ferrero De Loma-Osorio, JM.; Trénor Gomis, BA. (2014). In silico ischaemia-induced reentry at the Purkinjeventricle interface. EP-Europace. 16(3):444-451. https://doi.org/10.1093/europace/eut386S44445116
On the consistency of the peculiar GRBs 060218 and 060614 with the Ep,i - Eiso correlation
We analyze and discuss the position of GRB 060218 and GRB 060614 in the Ep,i
- Eiso plane. GRB 060218 is important because of its similarity with GRB
980425, the proto-type event of the GRB-SN connection. While GRB 980425 is an
outlier of the Ep,i - Eiso correlation, we find that GRB 060218 is fully
consistent with it. This evidence, combined with the `chromatic' behavior of
the afterglow light curves, is at odds with the hypothesis that GRB 060218 was
a `standard' GRB seen off-axis and supports the existence of a class of truly
sub--energetic GRBs. GRB 060614 is a peculiar event not accompanied by a bright
Supernova. Based on published spectral information, we find that also this
event is consistent with the Ep,i - Eiso correlation. We discuss the
implications of our results for the rate of sub--energetic GRBs, the GRB/SN
connection and the properties of the newly discovered sub-class of long GRBs
not associated with bright Supernovae. We have included in our analysis other
recent GRBs with clear evidence (or clear no evidence) of associated SNe.Comment: 9 pages, 4 figures, FINAL REVISED VERSION (added Figure 4 and short
GRBs in Figure 1; minor changes and style corrections applied; references
updated), submitted to A&A on November 25, 200
The Optical SN 2012bz Associated with the Long GRB 120422A
The association of Type Ic SNe with long-duration GRBs is well established.
We endeavor, through accurate ground-based observational campaigns, to
characterize these SNe at increasingly high redshifts. We obtained a series of
optical photometric and spectroscopic observations of the Type Ic SN2012bz
associated with the Swift long-duration GRB120422A (z=0.283) using the 3.6-m
TNG and the 8.2-m VLT telescopes. The peak times of the light curves of
SN2012bz in various optical filters differ, with the B-band and i'-band light
curves reaching maximum at ~9 and ~23 rest-frame days, respectively. The
bolometric light curve has been derived from individual bands photometric
measurements, but no correction for the unknown contribution in the
near-infrared (probably around 10-15%) has been applied. Therefore, the present
light curve should be considered as a lower limit to the actual UV-optical-IR
bolometric light curve. This pseudo-bolometric curve reaches its maximum (Mbol
= -18.56 +/- 0.06) at 13 +/- 1 rest-frame days; it is similar in shape and
luminosity to the bolometric light curves of the SNe associated with z<0.2 GRBs
and more luminous than those of SNe associated with XRFs. A comparison with the
model generated for the bolometric light curve of SN2003dh suggests that
SN2012bz produced only about 15% less 56Ni than SN2003dh, about 0.35 Msol.
Similarly the VLT spectra of SN2012bz, after correction for Galactic extinction
and for the contribution of the host galaxy, suggest comparable explosion
parameters with those observed in SN2003dh (EK~3.5 x 10^52 erg, Mej~7 Msol) and
a similar progenitor mass (~25-40 Msol). GRB120422A is consistent with the
Epeak-Eiso and the EX,iso-Egamma,iso-E_peak relations. GRB120422A/SN2012bz
shows the GRB-SN connection at the highest redshift so far accurately monitored
both photometrically and spectroscopically.Comment: 7 pages, 6 figures, 2 tables, accepted for publication in Astronomy &
Astrophysic
Transition from Fireball to Poynting-flux-dominated Outflow in Three-Episode GRB 160625B
The ejecta composition is an open question in gamma-ray bursts (GRB) physics.
Some GRBs possess a quasi-thermal spectral component in the time-resolved
spectral analysis, suggesting a hot fireball origin. Others show a featureless
non-thermal spectrum known as the "Band" function, consistent with a
synchrotron radiation origin and suggesting that the jet is
Poynting-flux-dominated at the central engine and likely in the emission region
as well. There are also bursts showing a sub-dominant thermal component and a
dominant synchrotron component suggesting a likely hybrid jet composition. Here
we report an extraordinarily bright GRB 160625B, simultaneously observed in
gamma-rays and optical wavelengths, whose prompt emission consists of three
isolated episodes separated by long quiescent intervals, with the durations of
each "sub-burst" being 0.8 s, 35 s, and 212 s, respectively. Its high
brightness (with isotropic peak luminosity L
erg/s) allows us to conduct detailed time-resolved spectral analysis in each
episode, from precursor to main burst and to extended emission. The spectral
properties of the first two sub-bursts are distinctly different, allowing us to
observe the transition from thermal to non-thermal radiation between
well-separated emission episodes within a single GRB. Such a transition is a
clear indication of the change of jet composition from a fireball to a
Poynting-flux-dominated jet.Comment: Revised version reflecting the referees' comments. 27 pages, 11
figures, 5 tables. The final edited version will appear in Nature Astronom
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