The Younger Dryas black mat from Ojo de Agua, a geoarchaeologicalsite in Northeastern Zacatecas, Mexico

New explorations in the desert of northeastern Zacatecas, in central-northern Mexico, revealed dozens ofarchaeological and geoarchaeological sites. One of them, Ojo de Agua, contains the remains of a Pleis-tocene spring-fed hydrographic system located at the southeastern end of a large elongated endorheicbasin. The locality yielded a particularly dark, highly organic stratigraphic layer commonly known in theAmericas as Black Mat (BM), exposed on the natural profiles of a creek, but not associated with culturalmaterials. Several radiocarbon assessments confirmed the formation of the Ojo de Agua Black Mat duringthe Younger Dryas chronozone, with ten calibrated results clustering between 12,700e12,100 cal BP. Thismulti-proxy study confirmed the peculiarity of the deposit and found similarities and differences withother contexts of Younger Dryas age. The Ojo de Agua Black Mat (stratum C2) is far richer in charcoalspecks than the related strata, but lacks phytoliths, diatoms or ostracods. No further biological remainswere found in it, except for intrusive capillary roots. Clearly water-lain in a shallow pond, the stratumqualifies as a clayey silt with an acidic-to-neutral pH. Rich in heavy metals and with high contents oftitanium, the Ojo de Agua Black Mat yielded significant indicators of intense wildfires during the YoungerDryas, but produced no carbon spherules or nanodiamonds supposedly linked to the impact theory

Volume estimation of soil stored in agricultural terrace systems : a geomorphometric approach

High-resolution topographic (HRT) techniques allow the mapping and characterization of geomorphological features with wide-ranging perspectives at multiple scales. We can exploit geomorphometric information in the study of the most extensive and common landforms that humans have ever produced: agricultural terraces. We can only develop an understanding of these historical landform through in-depth knowledge of their origin, evolution and current state in the landscape. These factors can ultimately assist in the future preservation of such landforms in a world increasingly affected by anthropogenic activities. From HRT surveys, it is possible to produce high-resolution Digital Terrain Models (DTMs) from which important geomorphometric parameters such as topographic curvature, to identify terrace edges can be extracted, even if abandoned or covered by uncontrolled vegetation. By using riser bases as well as terrace edges (riser tops) and through the computation of minimum curvature, it is possible to obtain environmentally useful information on these agricultural systems such as terrace soil thickness and volumes. The quantification of terrace volumes can provide new benchmarks for soil erosion models, new perspectives to stakeholders for terrace management in terms of natural hazard and offer a measure of the effect of these agricultural systems on soil organic carbon sequestration. This paper presents the realization and testing of an innovative and rapid methodological workflow to estimate the anthropogenic reworked and moved soil of terrace systems in different landscapes. We start with remote terrace mapping at large scale and then utilize more detailed HRT surveys to extract geomorphological features, from which the original theoretical slope-surface of terrace systems were derived. These last elements were compared with sub-surface information obtained from the excavations across the study sites that confirm the reliability of the methodology used. The results of this work have produced accurate DTMs of Difference (DoD) for three terrace sites in central Europe in Italy and Belgium. Differences between actual and theoretical terraces from DTM and excavation evidence have been used to estimate the soil volumes and masses used to remould slopes. The utilization of terrace and lynchet volumetric data, enriched by geomorphometric analysis through indices such as sediment conductivity provides a unique and efficient methodology for the greater understanding of these globally important landforms, in a period of increasing land pressure

Early to Middle Bronze Age agricultural terraces in north-east England : morphology, dating and cultural implications

Terracing is found widely in the Mediterranean and in other hilly and mountainous regions of the world. Yet while archaeological attention to these ‘mundane' landscape features has grown, they remain understudied, particularly in Northern Europe. Here, the authors present a multidisciplinary study of terraces in the Breamish Valley, Northumberland. The results date their construction to the Early to Middle Bronze Age, when they were built by cutting back the hillside, stone clearance and wall construction. Environmental evidence points to their use for cereal cultivation. The authors suggest that the construction and use of these terraces formed part of an Early to Middle Bronze Age agricultural intensification, which may have been both demographically and culturally driven

Integrated Molecular Meta-Analysis of 1,000 Pediatric High-Grade and Diffuse Intrinsic Pontine Glioma.

We collated data from 157 unpublished cases of pediatric high-grade glioma and diffuse intrinsic pontine glioma and 20 publicly available datasets in an integrated analysis of >1,000 cases. We identified co-segregating mutations in histone-mutant subgroups including loss of FBXW7 in H3.3G34R/V, TOP3A rearrangements in H3.3K27M, and BCOR mutations in H3.1K27M. Histone wild-type subgroups are refined by the presence of key oncogenic events or methylation profiles more closely resembling lower-grade tumors. Genomic aberrations increase with age, highlighting the infant population as biologically and clinically distinct. Uncommon pathway dysregulation is seen in small subsets of tumors, further defining the molecular diversity of the disease, opening up avenues for biological study and providing a basis for functionally defined future treatment stratification

Aridification of the Egyptian Sahara 5000–4000 cal BP revealed from x-ray fluorescence analysis of Nile Delta sediments at Kom al-Ahmer/Kom Wasit

Elemental XRF analysis carried out on an 8m long core from the Nile Delta reveals a gradual increase in the Ca/Ti ratio between 5000–4000 cal BP which is linked to the progressive development of hyper-aridity in this region. The increase results from elevated flux of aeolian material entering the Nile river system from calcareous source rock geologies in the dryer Egyptian Sahara. The most major increase in hyper-aridity occurs around 4000 cal BP. Such a perspective suggests a locally abrupt, regionally time-transgressive inception of hyper-aridity in this region at the end of the African Humid Period. After this time, reorganisation of wind circulation meant that less Saharan-derived aeolian material entered the Nile Valley, and the contribution of aeolian material in the Nile’s sedimentary signal was also dwarfed by an increase in Blue Nile sedimentary flux. Chronological control is provided by two radiocarbon dates and the top and bottom of a well-constrained pottery horizon that dates from the period of occupation of two nearby archaeological sites: Kom al-Ahmer and Kom Wasit.</p

Using Conceptual Structures in the Design of Computer-based Assessment Software

Abstract. This paper discusses the use of conceptual structures in the design of computer-based assessment (CBA) tools for e-assessment of programming exercises. In STEM (science, technology, engineering and maths) subjects, universities often observe high dropout and failure rates among the first year students. There are a number of research initiatives that investigate the use of interactive teaching methods and e-learning technologies for improving STEM education. This paper presents a conceptual model of programming exercises and discusses more generally how conceptual structures can be employed for the implementation of CBA tools.

Polynucleotide Phosphorylase Activity May Be Modulated by Metabolites in Escherichia coli*♦

RNA turnover is an essential element of cellular homeostasis and response to environmental change. Whether the ribonucleases that mediate RNA turnover can respond to cellular metabolic status is an unresolved question. Here we present evidence that the Krebs cycle metabolite citrate affects the activity of Escherichia coli polynucleotide phosphorylase (PNPase) and, conversely, that cellular metabolism is affected widely by PNPase activity. An E. coli strain that requires PNPase for viability has suppressed growth in the presence of increased citrate concentration. Transcriptome analysis reveals a PNPase-mediated response to citrate, and PNPase deletion broadly impacts on the metabolome. In vitro, citrate directly binds and modulates PNPase activity, as predicted by crystallographic data. Binding of metal-chelated citrate in the active site at physiological concentrations appears to inhibit enzyme activity. However, metal-free citrate is bound at a vestigial active site, where it stimulates PNPase activity. Mutagenesis data confirmed a potential role of this vestigial site as an allosteric binding pocket that recognizes metal-free citrate. Collectively, these findings suggest that RNA degradative pathways communicate with central metabolism. This communication appears to be part of a feedback network that may contribute to global regulation of metabolism and cellular energy efficiency