Traditional professions+ elements of tourist integrated development in rural areas

Integrated development seen at the level of local community assumes a global approach of all influence factors over these on the one hand, but also the evolution of these in time on another hand. In these conditions there must be taken in consideration a series of aspects as: economic activities, local community and environment protection. The purpose of this paper is to analyze traditional trades/professions from rural areas in order to surprise these interconnections with environmental factors, economic aspects and local community. Traditional trades can be looked at from many points of view. First of them and the oldest one is the economic point of view. Employed populations in traditional fields are insuring a source of income and contribute to a raise of local wellbeing. The second aspect is bound by the attraction possibility of tourists in the area through the angle of these original activities, which are ever rarer due to the economic progress and competition. A third aspect, under which we are going to follow the trades, is referring to the development of the feeling of pride and community belonging, the maintaining of youth in the locality, at valuating the customs and folk traditions as a first step towards the perpetuation of these in the future. Last but not least we will analyze the connections between traditional trades and the natural framework as well as the impact of the above mentioned on the environment.integrated tourism, local community, rural, traditional handicrafts, customs

Tumour‐stage mycosis fungoides regressing with milia and pustules after total skin electron beam therapy

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/139098/1/jdv14372.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/139098/2/jdv14372_am.pd

Altered fMRI restingâ state connectivity in individuals with fibromyalgia on acute pain stimulation

BackgroundFibromyalgia is a chronic widespread pain condition, with patients commonly reporting other symptoms such as sleep difficulties, memory complaints and fatigue. The use of magnetic resonance imaging (MRI) in fibromyalgia has allowed for the detection of neural abnormalities, with alterations in brain activation elicited by experimental pain and alterations in resting state connectivity related to clinical pain.MethodsIn this study, we sought to monitor state changes in resting brain connectivity following experimental pressure pain in fibromyalgia patients and healthy controls. Twelve fibromyalgia patients and 15 healthy controls were studied by applying discrete pressure stimuli to the thumbnail bed during MRI. Restingâ state functional MRI scanning was performed before and immediately following experimental pressure pain. We investigated changes in functional connectivity to the thalamus and the insular cortex.ResultsAcute pressure pain increased insula connectivity to the anterior cingulate and the hippocampus. Additionally, we observed increased thalamic connectivity to the precuneus/posterior cingulate cortex, a known part of the default mode network, in patients but not in controls. This connectivity was correlated with changes in clinical pain.ConclusionsThese data reporting changes in restingâ state brain activity following a noxious stimulus suggest that the acute painful stimuli may contribute to the alteration of the neural signature of chronic pain.What does this study/add?In this study acute pain application shows an echo in functional connectivity and clinical pain changes in chronic pain.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/122449/1/ejp832_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/122449/2/ejp832.pd

Оценка знаний семейных врачей сектора Ботаника, муниципия Кишинэу о деятельности первичной медицинской помощи и содержании Национальных Клинических Протоколов для взрослых

Nicolae Testemitanu State Medical and Pharmaceutical University, Territorial Medical Association Botanica, Chisinau, Congresul III al Medicilor de Familie din Republica Moldova, 17–18 mai, 2012, Chişinău, Republica Moldova, Conferinţa Naţională „Maladii bronhoobstructive la copii”, consacrată profesorului universitar, doctor habilitat Victor Gheţeul, 27 aprilie, Chişinău, Republica MoldovaThe quality of the primary health care is largely determined by the level of the professional training of family doctors. With the purpose of the quality improvement and standardization of the medical services rendered to the population of the Republic of Moldova, there have been developed the National clinical protocols for adults, which are designed as basis for the institutional protocols. There are more than 160 protocols in the process of implementation in clinical practice. The aim of the study was to assess the level of knowledge on primary health care activities and on the content of a number of National Clinical Protocols for adults, at the family physicians from Botanica district, mun. Chisinau. The results showed a higher level of knowledge of those National Clinical Protocols, for which implementation, doctors took some training programs and spent more time using them in clinical practice. The system of the survey of knowledge is an accessible method for estimating the degree of the implementation of the National Clinical Protocols. In order to accelerate their implementation, the topics of the National clinical protocols should be included in the post-graduate medical education programs.Качество первичной медицинской помощи во многом определяется уровнем профессиональной подготовки семейных врачей. С этой целью и для стандартизации медицинских услуг оказываемых населению Республики Молдова были разработаны Национальные клинические протоколы для взрослых, на основании которых разработаны учрежденческие протоколы. В процессе внедрения в клиническую практику находятся более 160 протоколов. Цель данного исследования: оценка уровня знаний семейных врачей сектора Ботаника, муниципия Кишинэу о деятельности первичной медицинской помощи и содержании ряда Национальных клинических протоколов для взрослых. Результаты продемонстрировали более высокий уровень знания Национальных клинических протоколов, по внедрению которых врачи проходили какие-либо обучающие программы, и которым уделялось больше времени в клинической практике. Система опроса знаний – доступный метод оценки степени внедрения Национальных клинических протоколов. В программах постуниверситетского образования врачей следует продолжить изучение Национальных клинических протоколов для ускорения процесса их внедрения

A whole-genome assembly of the domestic cow, Bos taurus

Background: The genome of the domestic cow, Bos taurus, was sequenced using a mixture of hierarchical and whole-genome shotgun sequencing methods. Results: We have assembled the 35 million sequence reads and applied a variety of assembly improvement techniques, creating an assembly of 2.86 billion base pairs that has multiple improvements over previous assemblies: it is more complete, covering more of the genome; thousands of gaps have been closed; many erroneous inversions, deletions, and translocations have been corrected; and thousands of single-nucleotide errors have been corrected. Our evaluation using independent metrics demonstrates that the resulting assembly is substantially more accurate and complete than alternative versions. Conclusions: By using independent mapping data and conserved synteny between the cow and human genomes, we were able to construct an assembly with excellent large-scale contiguity in which a large majority (approximately 91%) of the genome has been placed onto the 30 B. taurus chromosomes. We constructed a new cow-human synteny map that expands upon previous maps. We also identified for the first time a portion of the B. taurus Y chromosome. © 2009 Zimin et al.; licensee BioMed Central Ltd

Internet of things

Manual of Digital Earth / Editors: Huadong Guo, Michael F. Goodchild, Alessandro Annoni .- Springer, 2020 .- ISBN: 978-981-32-9915-3Digital Earth was born with the aim of replicating the real world within the digital world. Many efforts have been made to observe and sense the Earth, both from space (remote sensing) and by using in situ sensors. Focusing on the latter, advances in Digital Earth have established vital bridges to exploit these sensors and their networks by taking location as a key element. The current era of connectivity envisions that everything is connected to everything. The concept of the Internet of Things(IoT)emergedasaholisticproposaltoenableanecosystemofvaried,heterogeneous networked objects and devices to speak to and interact with each other. To make the IoT ecosystem a reality, it is necessary to understand the electronic components, communication protocols, real-time analysis techniques, and the location of the objects and devices. The IoT ecosystem and the Digital Earth (DE) jointly form interrelated infrastructures for addressing today’s pressing issues and complex challenges. In this chapter, we explore the synergies and frictions in establishing an efficient and permanent collaboration between the two infrastructures, in order to adequately address multidisciplinary and increasingly complex real-world problems. Although there are still some pending issues, the identified synergies generate optimism for a true collaboration between the Internet of Things and the Digital Earth

Castor bean organelle genome sequencing and worldwide genetic diversity analysis

Castor bean is an important oil-producing plant in the Euphorbiaceae family. Its high-quality oil contains up to 90% of the unusual fatty acid ricinoleate, which has many industrial and medical applications. Castor bean seeds also contain ricin, a highly toxic Type 2 ribosome-inactivating protein, which has gained relevance in recent years due to biosafety concerns. In order to gain knowledge on global genetic diversity in castor bean and to ultimately help the development of breeding and forensic tools, we carried out an extensive chloroplast sequence diversity analysis. Taking advantage of the recently published genome sequence of castor bean, we assembled the chloroplast and mitochondrion genomes extracting selected reads from the available whole genome shotgun reads. Using the chloroplast reference genome we used the methylation filtration technique to readily obtain draft genome sequences of 7 geographically and genetically diverse castor bean accessions. These sequence data were used to identify single nucleotide polymorphism markers and phylogenetic analysis resulted in the identification of two major clades that were not apparent in previous population genetic studies using genetic markers derived from nuclear DNA. Two distinct sub-clades could be defined within each major clade and large-scale genotyping of castor bean populations worldwide confirmed previously observed low levels of genetic diversity and showed a broad geographic distribution of each sub-clade

Working principle and demonstrator of microwave-multiplexing for the HOLMES experiment microcalorimeters

The determination of the neutrino mass is an open issue in modern particle physics and astrophysics. The direct mass measurement is the only theory-unrelated experimental tool capable to probe such quantity. The HOLMES experiment aims to measure the end-point energy of the electron capture (EC) decay of $^{163}$Ho with a statistical sensitivity on the neutrino mass as low as $\sim 1$ eV/c$^2$. In order to acquire the large needed statistics, by keeping the pile-up contribution as low as possible, 1024 transition edge sensors (TESs) with high energy and time resolutions will be employed. Microcalorimeter and bolometer arrays based on transition edge sensor with thousands of pixels are under development for several space-based and ground-based applications, including astrophysics, nuclear and particle physics, and materials science. The common necessary challenge is to develop pratical multiplexing techniques in order to simplify the cryogenics and readout systems. Despite the various multiplexing variants which are being developed have been successful, new approaches are needed to enable scaling to larger pixel counts and faster sensors, as requested for HOLMES, reducing also the cost and complexity of readout. A very novel technique that meets all of these requirements is based on superconducting microwave resonators coupled to radio-frequency Superconducting Quantum Interference Devices, in which the the changes in the TES input current is tranduced to a change in phase of a microwave signal. In this work we introduce the basics of this technique, the design and development of the first two-channel read out system and its performances with the first TES detectors specifically designed for HOLMES. In the last part we explain how to extend this approach scaling to 1024 pixels.Comment: accepted on JINS

The genomes of two key bumblebee species with primitive eusocial organization

Background: The shift from solitary to social behavior is one of the major evolutionary transitions. Primitively eusocial bumblebees are uniquely placed to illuminate the evolution of highly eusocial insect societies. Bumblebees are also invaluable natural and agricultural pollinators, and there is widespread concern over recent population declines in some species. High-quality genomic data will inform key aspects of bumblebee biology, including susceptibility to implicated population viability threats. Results: We report the high quality draft genome sequences of Bombus terrestris and Bombus impatiens, two ecologically dominant bumblebees and widely utilized study species. Comparing these new genomes to those of the highly eusocial honeybee Apis mellifera and other Hymenoptera, we identify deeply conserved similarities, as well as novelties key to the biology of these organisms. Some honeybee genome features thought to underpin advanced eusociality are also present in bumblebees, indicating an earlier evolution in the bee lineage. Xenobiotic detoxification and immune genes are similarly depauperate in bumblebees and honeybees, and multiple categories of genes linked to social organization, including development and behavior, show high conservation. Key differences identified include a bias in bumblebee chemoreception towards gustation from olfaction, and striking differences in microRNAs, potentially responsible for gene regulation underlying social and other traits. Conclusions: These two bumblebee genomes provide a foundation for post-genomic research on these key pollinators and insect societies. Overall, gene repertoires suggest that the route to advanced eusociality in bees was mediated by many small changes in many genes and processes, and not by notable expansion or depauperation

Genetic and phenotypic characterization of NKX6‐2‐related spastic ataxia and hypomyelination

Background and purpose Hypomyelinating leukodystrophies are a heterogeneous group of genetic disorders with a wide spectrum of phenotypes and a high rate of genetically unsolved cases. Bi‐allelic mutations in NKX6‐2 were recently linked to spastic ataxia 8 with hypomyelinating leukodystrophy. Methods Using a combination of homozygosity mapping, exome sequencing, and detailed clinical and neuroimaging assessment a series of new NKX6‐2 mutations in a multicentre setting is described. Then, all reported NKX6‐2 mutations and those identified in this study were combined and an in‐depth analysis of NKX6‐2‐related disease spectrum was provided. Results Eleven new cases from eight families of different ethnic backgrounds carrying compound heterozygous and homozygous pathogenic variants in NKX6‐2 were identified, evidencing a high NKX6‐2 mutation burden in the hypomyelinating leukodystrophy disease spectrum. Our data reveal a phenotype spectrum with neonatal onset, global psychomotor delay and worse prognosis at the severe end and a childhood onset with mainly motor phenotype at the milder end. The phenotypic and neuroimaging expression in NKX6‐2 is described and it is shown that phenotypes with epilepsy in the absence of overt hypomyelination and diffuse hypomyelination without seizures can occur. Conclusions NKX6‐2 mutations should be considered in patients with autosomal recessive, very early onset of nystagmus, cerebellar ataxia with hypotonia that rapidly progresses to spasticity, particularly when associated with neuroimaging signs of hypomyelination. Therefore, it is recommended that NXK6‐2 should be included in hypomyelinating leukodystrophy and spastic ataxia diagnostic panels