Tunable entanglement distillation of spatially correlated down-converted photons

We report on a new technique for entanglement distillation of the bipartite continuous variable state of spatially correlated photons generated in the spontaneous parametric down-conversion process (SPDC), where tunable non-Gaussian operations are implemented and the post-processed entanglement is certified in real-time using a single-photon sensitive electron multiplying CCD (EMCCD) camera. The local operations are performed using non-Gaussian filters modulated into a programmable spatial light modulator and, by using the EMCCD camera for actively recording the probability distributions of the twin-photons, one has fine control of the Schmidt number of the distilled state. We show that even simple non-Gaussian filters can be finely tuned to a ~67% net gain of the initial entanglement generated in the SPDC process.Comment: 12 pages, 6 figure

Integrated multi-assay culture model for stem cell chondrogenic differentiation

Recent osteochondral repair strategies highlight the promise of mesenchymal progenitors, an accessible stem cell source with osteogenic and chondrogenic potential, used in conjunction with biomaterials for tissue engineering. For this, regenerative medicine approaches require robust models to ensure selected cell populations can generate the desired cell type in a reproducible and measurable manner. Techniques for in vitro chondrogenic differentiation are well-established but largely qualitative, relying on sample staining and imaging. To facilitate the in vitro screening of pro-chondrogenic treatments, a 3D micropellet culture combined with three quantitative GAG assays has been developed, with a fourth parallel assay measuring sample content to enable normalisation. The effect of transforming growth factor beta (TGF-β) used to validate this culture format produced a measurable increase in proteoglycan production in the parallel assays, in both 2D and 3D culture configurations. When compared to traditional micropellets, the monolayer format appeared less able to detect changes in cell differentiation, however in-well 3D cultures displayed a significant differential response. Effects on collagen 2 expression confirmed these observations. Based on these results, a microplate format was optimised for 3D culture, in a high-throughput in-well configuration. This model showed improved sensitivity and confirmed the 3D micropellet in-well quantitative assays as an effective differentiation format compatible with streamlined, high-throughput chondrogenic screens

Profiling of VEGFs and VEGFRs as Prognostic Factors in Soft Tissue Sarcoma: VEGFR-3 Is an Independent Predictor of Poor Prognosis

BACKGROUND: In non-gastrointestinal stromal tumor soft tissue sarcoma (non-GIST STS) optimal treatment is surgery with wide resection margins. Vascular endothelial growth factors (VEGFs) and receptors (VEGFRs) are known to be key players in the initiation of angiogenesis and lymphangiogenesis. This study investigates the prognostic impact of VEGFs and VEGFRs in non-GIST STS with wide and non-wide resection margins. METHODS: Tumor samples from 249 patients with non-GIST STS were obtained and tissue microarrays were constructed for each specimen. Immunohistochemistry was used to evaluate the expressions of VEGF-A, -C and -D and VEGFR-1, -2 and -3. RESULTS: In the univariate analyses, VEGF-A (P=0.040) in the total material, and VEGF-A (P=0.018), VEGF-C (P=0.025) and VEGFR-3 (P=0.027) in the subgroup with wide resection margins, were significant negative prognostic indicators of disease-specific survival (DSS). In the multivariate analysis, high expression of VEGFR-3 (P=0.042, HR=1.907, 95% CI 1.024-3.549) was an independent significant negative prognostic marker for DSS among patients with wide resection margins. CONCLUSION: VEGFR-3 is a strong and independent negative prognostic marker for non-GIST STSs with wide resection margins

X-ray rotational modulation of a supersaturated star in IC 2391

We present evidence of X-ray rotational modulation on VXR45, a young fast rotator star, member of IC 2391. It is a dG9 spectral type star whose rotational period and X-ray luminosity make it a supersaturated star. Our X-ray observation, made with EPIC/PN on XMM-Newton, covers about two photometric rotational periods. The detection of rotational modulation implies the presence of structural inhomogeneities. Possible interpretations are presented and discussed.Comment: 4 pages with 4 PostScript figures. Accepted for publication in Astronomy & Astrophysics Letter

Auto-driven Photo Elicitation Interviews in Research with Children: Ethical and Practical Considerations

Children can sometimes find it difficult to articulate their experiences if they have to rely solely on words. Giving children the opportunity to use arts-based research approaches can support their participation in research and create a bridge that enables them to express their perspectives and feelings. This paper focuses on the ethical and practical considerations when using photo elicitation interviews (PEI) in research with children. The discussion and examples provided are drawn from an international study that used auto-driven PEI, where photographs are taken by children themselves, to explore children’s experiences of living with a chronic condition and the impact condition management may have on their everyday lives. In this paper we critically explore the issues arising from our use of PEI including children’s participation and engagement, balancing power and control, and keeping children safe. The main areas of focus for the paper are how PEI provided a means of shifting control; how setting photographic boundaries influenced our PEI study with children; and how we addressed risks associated with the method. Our experience shows that PEI is an engaging and valuable research method, providing a powerful medium for obtaining rich data with children. However, PEI is challenging and it requires researchers to conscientiously address ethical and practical aspects that extend beyond those inherent to standard (words-alone) interviews

Democracy Matters: Lessons from the 2015 Citizens' Assemblies on English Devolution

The Citizens’ Assembly pilots on local democracy and devolution were the first of their kind in the United Kingdom. Organised by Democracy Matters — an alliance of university researchers and civil society organisations led by Professor Matthew Flinders — and funded by the UK’s Economic and Social Research Council, the Assemblies took place in Southampton and Sheffield towards the end of 2015

Topography of the Dolomites modulates range dynamics of narrow endemic plants under climate change

open9noClimate change is expected to threaten endemic plants in the Alps. In this context, the factors that may modulate species responses are rarely investigated at a local scale. We analyzed eight alpine narrow endemics of the Dolomites (southeastern Alps) under different predicted climate change scenarios at fine spatial resolutions. We tested possible differences in elevation, topographic heterogeneity and velocity of climate change among areas of gained, lost, or stable climatic habitat. The negative impact of climate change ranged from moderate to severe, depending on scenario and species. Generally, range loss occurred at the lowest elevations, while gained and stable areas were located at highest elevations. For six of the species, climate change velocity had higher values in stable and gained areas than in lost ones. Our findings support the role of topographic heterogeneity in maintaining climatic microrefugia, however, the peculiar topography of the Dolomites, characterized by high altitude plateaus, resulted in high climate change velocity in areas of projected future climatic suitability. Our study supports the usefulness of multiple predictors of spatio-temporal range dynamics for regional climate-adapted management and eventual assisted colonization planning to not overlook or overestimate the potential impact of climate change locally.openRota F.; Casazza G.; Genova G.; Midolo G.; Prosser F.; Bertolli A.; Wilhalm T.; Nascimbene J.; Wellstein C.Rota, F.; Casazza, G.; Genova, G.; Midolo, G.; Prosser, F.; Bertolli, A.; Wilhalm, T.; Nascimbene, J.; Wellstein, C