The design of CO2-based working fluids for high-temperature heat source power cycles

The application of CO2power cycles is advantageous to exploit high-temperature sources (500-800°C) in the case of available low-temperature heat sinks (15-25°C). However, their efficiency is strongly reduced for higher heat sink temperatures. At these temperatures, due to the low-critical temperature of CO2(about 31°C), CO2is in fact compressed in the supercritical vapor phase rather than in the liquid phase, thus increasing energetic demand for compression. One of the solutions envisaged to overcome this problem is the addition of one or more chemicals that allow having a mixture with a higher critical temperature than the one of pure CO2. This preserve the working fluid compression in its liquid phase, even in the case of heat sinks with temperatures greater than 25°C. This research shows that the addition to CO2of a properly selected chemical component enables to increase the critical temperature up to 45°C with relevant improvements of cycle efficiency with respect to pure-CO2power cycles. In particular, it summarizes the most relevant criteria to be accounted for when selecting CO2-additives. Moreover, the paper warns of the thermodynamic effects deriving from adding to CO2a second characterized by a much more high critical temperature, such as the occurrence of infinite-pressure critical points and multiple-phase liquid-liquid and vapor-liquid critical points. Finally, the paper analyses the thermodynamic properties of a high-critical temperature CO2-based mixture, suitable for these applications, that presents multiple phase critical points. In this regard, it is specified that the paper also aims at filling a knowledge gap in the study of thermodynamic properties of mixtures presenting how do enthalpy and specific volume change in response to pressure variations in the event of liquid-liquid and vapour-liquid critical points. Finally, we present the comparison between performances of power cycles which use, as working fluid, either pure CO2or the novel designed higher temperature CO2-based mixture

Removing noise from pyrosequenced amplicons

Background In many environmental genomics applications a homologous region of DNA from a diverse sample is first amplified by PCR and then sequenced. The next generation sequencing technology, 454 pyrosequencing, has allowed much larger read numbers from PCR amplicons than ever before. This has revolutionised the study of microbial diversity as it is now possible to sequence a substantial fraction of the 16S rRNA genes in a community. However, there is a growing realisation that because of the large read numbers and the lack of consensus sequences it is vital to distinguish noise from true sequence diversity in this data. Otherwise this leads to inflated estimates of the number of types or operational taxonomic units (OTUs) present. Three sources of error are important: sequencing error, PCR single base substitutions and PCR chimeras. We present AmpliconNoise, a development of the PyroNoise algorithm that is capable of separately removing 454 sequencing errors and PCR single base errors. We also introduce a novel chimera removal program, Perseus, that exploits the sequence abundances associated with pyrosequencing data. We use data sets where samples of known diversity have been amplified and sequenced to quantify the effect of each of the sources of error on OTU inflation and to validate these algorithms

On the randomised stability constant for inverse problems

International audienceIn this paper we introduce the randomised stability constant for abstract inverse problems, as a generalisation of the randomised observability constant, which was studied in the context of observability inequalities for the linear wave equation. We study the main properties of the randomised stability constant and discuss the implications for the practical inversion, which are not straightforward

Anatomy of an exhumed debrite and impact on stratal architecture

Submarine debrites generate complicated patterns of seabed relief which in uence subsequent ow behaviour and depositional patterns. However, recognizing this interaction in subsurface data is challenging in seismic and well data. To bridge this resolution gap, large-scale outcrop analogues can be used. The early post-rift Middle Jurassic succession of the Los Molles Formation is well-exposed along a 10 km long and downdip-orientated W-E outcrop belt located the western Central Neuquen Basin, Argentina.Fil: Martanez Donate, Ander. University of Manchester; Reino UnidoFil: Privat, Aurelia. University of Leeds; Reino UnidoFil: Spychala, Yvonne. Leibniz Universitat Hannover; AlemaniaFil: Hodgson, David. University of Leeds; Reino UnidoFil: Jackson, Christopher A. L.. Imperial College London; Reino UnidoFil: Kane, Ian. University of Manchester; Reino UnidoFil: Schwarz, Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Geológicas. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Investigaciones Geológicas; ArgentinaFil: Flint, Stephen S.. University of Manchester; Reino Unido59th British Sedimentological Research Group Annual General MeetingLiverpoolReino UnidoBritish Sedimentological Research GroupUniversity of Liverpoo

Influence of particle composition and thermal cycling on bijel formation

Colloidal particles with appropriate wetting properties can become very strongly trapped at an interface between two immiscible fluids. We have harnessed this phenomenon to create a new class of soft materials with intriguing and potentially useful characteristics. The material is known as a bijel: bicontinuous interfacially-jammed emulsion gel. It is a colloid-stabilized emulsion with fluid-bicontinuous domains. The potential to create these gels was first predicted using computer simulations. Experimentally we use mixtures of water and 2,6-lutidine at the composition for which the system undergoes a critical demixing transition on warming. Colloidal silica, with appropriate surface chemistry, is dispersed while the system is in the single-fluid phase; the composite sample is then slowly warmed well beyond the critical temperature. The liquids phase separate via spinodal decomposition and the particles become swept up on the newly created interfaces. As the domains coarsen the interfacial area decreases and the particles eventually become jammed together. The resulting structures have a significant yield stress and are stable for many months. Here we begin to explore the complex wetting properties of fluorescently-tagged silica surfaces in water-lutidine mixtures, showing how they can be tuned to allow bijel creation. Additionally we demonstrate how the particle properties change with time while they are immersed in the solvents.Comment: Proceedings of the 7th Liquid Matter Conference, held in Lund (Sweden) in June 200

Cluster Analysis and Comparison of Various Chloroplast Transcriptomes and Genes in Arabidopsis thaliana

Chloroplast RNA metabolism is integrated into wider gene regulatory networks. To explore how, we performed a chloroplast genome-wide expression analysis on numerous nuclear Arabidopsis mutants affected in diverse chloroplast functions and wild-type plants subjected to various stresses and conditions. On the basis of clustering analysis, plastid genes could be divided into two oppositely regulated clusters, largely congruent with known targets of nucleus- and plastid-encoded RNA polymerases, respectively. Further eight sub-clusters contained co-transcribed and functionally tightly associated genes. The chloroplast transcriptomes could also be classified into two major groups comprising mutants preferentially affected in general plastid gene expression and other chloroplast functions, respectively. Deviations from characteristic expression profiles of transcriptomes served to identify novel mutants impaired in accumulation and/or processing of specific plastid RNAs. Expression profiles were useful to distinguish albino mutants affected in plastid gene expression from those with defects in other plastid functions. Remarkably, biotic and abiotic stressors did not define transcriptionally determined clusters indicating that post-transcriptional regulation of plastid gene expression becomes more important under changing environmental conditions. Overall, the identification of sets of co-regulated genes provides insights into the integration of plastid gene expression into common pathways that ensures a coordinated response

“Savages Who Speak French”: Folklore, Primitivism and Morals in Robert Hertz

Hertz's analysis of the Alpine cult of Saint Besse apparently marks a break from his studies of death, sin and the left to folkloric studies. This analysis helps one to understand the personality of Robert Hertz. His sociological curiosity about folklore reveals his ambiguous position in social sciences at the beginning of the twentieth century. His text appears to be a variation from the Durkheimian norm, but another reading could suggest that Hertz continued and went beyond Durkheimian thought to something between sociology of the modern world and engaged socialism. Through this study, Hertz linked his political ideals, his work in ethnology and his desire for social involvement. The cult of Saint Besse perpetuated as much religious tradition as local identity. The Alpine people were presented in the text as wilful perpetuators of an ideal social order, whose loss for his contemporary city dwellers Hertz feared. The alpine Other, marked by a material and moral backwardness, represented for activist and socialist Hertz one of the paths of balanced social organization that stabilized the identity of a group across time if it fit rather well into the folkloric stereotypes of the beginning of the twentieth century. Finally, by linking events in Herz's life (e.g., the accidental Alpine death of his father), this article suggests that the legend of Saint Besse embodied several recurring motifs in Hertz' career: the accidental deaths of saint and father by falls, the military role of the saint and of Hertz himself

The human DNA glycosylases NEIL1 and NEIL3 excise psoralen-induced DNA-DNA cross-links in a four-stranded DNA structure

Interstrand cross-links (ICLs) are highly cytotoxic DNA lesions that block DNA replication and transcription by preventing strand separation. Previously, we demonstrated that the bacterial and human DNA glycosylases Nei and NEIL1 excise unhooked psoralen-derived ICLs in three-stranded DNA via hydrolysis of the glycosidic bond between the crosslinked base and deoxyribose sugar. Furthermore, NEIL3 from Xenopus laevis has been shown to cleave psoralen- and abasic site-induced ICLs in Xenopus egg extracts. Here we report that human NEIL3 cleaves psoralen-induced DNA-DNA cross-links in three-stranded and four-stranded DNA substrates to generate unhooked DNA fragments containing either an abasic site or a psoralen-thymine monoadduct. Furthermore, while Nei and NEIL1 also cleave a psoralen-induced four-stranded DNA substrate to generate two unhooked DNA duplexes with a nick, NEIL3 targets both DNA strands in the ICL without generating single-strand breaks. The DNA substrate specificities of these Nei-like enzymes imply the occurrence of long uninterrupted three- and four-stranded crosslinked DNA-DNA structures that may originate in vivo from DNA replication fork bypass of an ICL. In conclusion, the Nei-like DNA glycosylases unhook psoralen-derived ICLs in various DNA structures via a genuine repair mechanism in which complex DNA lesions can be removed without generation of highly toxic double-strand breaks

Substrate Entrainment, Depositional Relief, and Sediment Capture: Impact of a Submarine Landslide on Flow Process and Sediment Supply

From Frontiers via Jisc Publications RouterHistory: collection 2021, received 2021-08-12, accepted 2021-10-27, epub 2021-11-16Publication status: PublishedSubmarine landslides can generate complicated patterns of seafloor relief that influence subsequent flow behaviour and sediment dispersal patterns. In subsurface studies, the term mass transport deposits (MTDs) is commonly used and covers a range of processes and resultant deposits. While the large-scale morphology of submarine landslide deposits can be resolved in seismic reflection data, the nature of their upper surface and its impact on both facies distributions and stratal architecture of overlying deposits is rarely resolvable. However, field-based studies often allow a more detailed characterisation of the deposit. The early post-rift Middle Jurassic deep-water succession of the Los Molles Formation is exceptionally well-exposed along a dip-orientated WSW-ENE outcrop belt in the Chacay Melehue depocentre, Neuquén Basin, Argentina. We correlate 27 sedimentary logs constrained by marker beds to document the sedimentology and architecture of a >47 m thick and at least 9.6 km long debrite, which contains two different types of megaclasts. The debrite overlies ramps and steps, indicating erosion and substrate entrainment. Two distinct sandstone-dominated units overlie the debrite. The lower sandstone unit is characterised by: 1) abrupt thickness changes, wedging and progressive rotation of laminae in sandstone beds associated with growth strata; and 2) detached sandstone load balls within the underlying debrite. The combination of these features suggests syn-sedimentary foundering processes due to density instabilities at the top of the fluid-saturated mud-rich debrite. The debrite relief controlled the spatial distribution of foundered sandstones. The upper sandstone unit is characterised by thin-bedded deposits, locally overlain by medium-to thick-bedded lobe axis/off-axis deposits. The thin-beds show local thinning and onlapping onto the debrite, where it develops its highest relief. Facies distributions and stacking patterns record the progradation of submarine lobes and their complex interaction with long-lived debrite-related topography. The emplacement of a kilometre-scale debrite in an otherwise mud-rich basinal setting and accumulation of overlying sand-rich deposits suggests a genetic link between the mass-wasting event and transient coarse clastic sediment supply to an otherwise sand-starved part of the basin. Therefore, submarine landslides demonstrably impact the routing and behaviour of subsequent sediment gravity flows, which must be considered when predicting facies distributions and palaeoenvironments above MTDs in subsurface datasets