What Works in Conservation 2021

This book provides an assessment of the effectiveness of 2526 conservation interventions based on summarized scientific evidence. The 2021 edition contains substantial new material on bat conservation, terrestrial mammal conservation and marine and freshwater mammals, thus completing the evidence for all mammal species categories. Other chapters cover practical global conservation of primates, amphibians, bats, birds, forests, peatlands, subtidal benthic invertebrates, shrublands and heathlands, as well as the conservation of European farmland biodiversity and some aspects of enhancing natural pest control, enhancing soil fertility, management of captive animals and control of freshwater invasive species. It contains key results from the summarized evidence for each conservation intervention and an assessment of the effectiveness of each by international expert panels. The accompanying website www.conservationevidence.com describes each of the studies individually, and provides full references. This is the sixth author-approved edition of What Works in Conservation, which is revised on an annual basis

Resistance to coronavirus infection in amino peptidase N-deficient pigs

The alphacoronaviruses, transmissible gastroenteritis virus (TGEV) and Porcine epidemic diarrhea virus (PEDV) are sources of high morbidity and mortality in neonatal pigs, a consequence of dehydration caused by the infection and necrosis of enterocytes. The biological relevance of amino peptidase N (ANPEP) as a putative receptor for TGEV and PEDV in pigs was evaluated by using CRISPR/Cas9 to edit exon 2 of ANPEP resulting in a premature stop codon. Knockout pigs possessing the null ANPEP phenotype and age matched wild type pigs were challenged with either PEDV or TGEV. Fecal swabs were collected daily from each animal beginning 1 day prior to challenge with PEDV until the termination of the study. The presence of virus nucleic acid was determined by PCR. ANPEP null pigs did not support infection with TGEV, but retained susceptibility to infection with PEDV. Immunohistochemistry confirmed the presence of PEDV reactivity and absence of TGEV reactivity in the enterocytes lining the ileum in ANPEP null pigs. The different receptor requirements for TGEV and PEDV have important implications in the development of new genetic tools for the control of enteric disease in pigs

Emerging fungal pathogen Ophidiomyces ophiodiicola in wild European snakes

Snake fungal disease (SFD) is an emerging disease of conservation concern in eastern North America. $\textit{Ophidiomyces ophiodiicola}$, the causative agent of SFD, has been isolated from over 30 species of wild snakes from six families in North America. Whilst $\textit{O. ophiodiicola}$ has been isolated from captive snakes outside North America, the pathogen has not been reported from wild snakes elsewhere. We screened 33 carcasses and 303 moulted skins from wild snakes collected from 2010–2016 in Great Britain and the Czech Republic for the presence of macroscopic skin lesions and $\textit{O. ophiodiicola}$. The fungus was detected using real-time PCR in 26 (8.6%) specimens across the period of collection. Follow up culture and histopathologic analyses confirmed that both $\textit{O. ophiodiicola}$ and SFD occur in wild European snakes. Although skin lesions were mild in most cases, in some snakes they were severe and were considered likely to have contributed to mortality. Culture characterisations demonstrated that European isolates grew more slowly than those from the United States, and phylogenetic analyses indicated that isolates from European wild snakes reside in a clade distinct from the North American isolates examined. These genetic and phenotypic differences indicate that the European isolates represent novel strains of $\textit{O. ophiodiicola}$. Further work is required to understand the individual and population level impact of this pathogen in Europe.This work was undertaken as part of the Garden Wildlife Health project www.gardenwildlifehealth.org. Funding was provided by the UK Department for the Environment Food & Rural Affairs and Welsh Government through the Animal Plant & Health Agency’s Diseases of Wildlife Scheme Scanning Surveillance Programme (Project ED1600), the Esmée Fairbairn Foundation, the Universities Federation for Animal Welfare, and the U.S. Geological Survey

Suppression of charged particle production at large transverse momentum in central Pb-Pb collisions at sNN=2.76\sqrt{s_{\rm NN}} = 2.76 TeV

Inclusive transverse momentum spectra of primary charged particles in Pb-Pb collisions at $\sqrt{s_{_{\rm NN}}}$ = 2.76 TeV have been measured by the ALICE Collaboration at the LHC. The data are presented for central and peripheral collisions, corresponding to 0-5% and 70-80% of the hadronic Pb-Pb cross section. The measured charged particle spectra in $|\eta|<0.8$ and $0.3 < p_T < 20$ GeV/$c$ are compared to the expectation in pp collisions at the same $\sqrt{s_{\rm NN}}$, scaled by the number of underlying nucleon-nucleon collisions. The comparison is expressed in terms of the nuclear modification factor $R_{\rm AA}$. The result indicates only weak medium effects ($R_{\rm AA} \approx$ 0.7) in peripheral collisions. In central collisions, $R_{\rm AA}$ reaches a minimum of about 0.14 at $p_{\rm T}=6$-7GeV/$c$ and increases significantly at larger $p_{\rm T}$. The measured suppression of high-$p_{\rm T}$ particles is stronger than that observed at lower collision energies, indicating that a very dense medium is formed in central Pb-Pb collisions at the LHC.Comment: 15 pages, 5 captioned figures, 3 tables, authors from page 10, published version, figures at http://aliceinfo.cern.ch/ArtSubmission/node/98

Two-pion Bose-Einstein correlations in central Pb-Pb collisions at sNN\sqrt{s_{\rm NN}} = 2.76 TeV

The first measurement of two-pion Bose-Einstein correlations in central Pb-Pb collisions at $\sqrt{s_{\rm NN}} = 2.76$ TeV at the Large Hadron Collider is presented. We observe a growing trend with energy now not only for the longitudinal and the outward but also for the sideward pion source radius. The pion homogeneity volume and the decoupling time are significantly larger than those measured at RHIC.Comment: 17 pages, 5 captioned figures, 1 table, authors from page 12, published version, figures at http://aliceinfo.cern.ch/ArtSubmission/node/388

A Severe Lack of Evidence Limits Effective Conservation of the World's Primates

Threats to biodiversity are well documented. However, to effectively conserve species and their habitats, we need to know which conservation interventions do (or do not) work. Evidence-based conservation evaluates interventions within a scientific framework. The Conservation Evidence project has summarized thousands of studies testing conservation interventions and compiled these as synopses for various habitats and taxa. In the present article, we analyzed the interventions assessed in the primate synopsis and compared these with other taxa. We found that despite intensive efforts to study primates and the extensive threats they face, less than 1% of primate studies evaluated conservation effectiveness. The studies often lacked quantitative data, failed to undertake postimplementation monitoring of populations or individuals, or implemented several interventions at once. Furthermore, the studies were biased toward specific taxa, geographic regions, and interventions. We describe barriers for testing primate conservation interventions and propose actions to improve the conservation evidence base to protect this endangered and globally important taxon

A Role for VEGFR2 Activation in Endothelial Responses Caused by Barrier Disruptive OxPAPC Concentrations

Introduction: Oxidation products of 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphatidylcholine (OxPAPC) differentially modulate endothelial cell (EC) barrier function in a dose-dependent fashion. Vascular endothelial growth factor receptor-2 (VEGFR2) is involved in the OxPAPC-induced EC inflammatory activation. This study examined a role of VEGFR2 in barrier dysfunction caused by high concentrations of OxPAPC and evaluated downstream signaling mechanisms resulting from the effect of OxPAPC in EC from pulmonary and systemic circulation

What Works in Conservation 2018

This book provides an assessment of the effectiveness of 1277 conservation interventions based on summarized scientific evidence. The 2018 edition contains new chapters covering practical global conservation of primates, peatlands, shrublands and heathlands, management of captive animals as well as an extended chapter on control of freshwater invasive species. Other chapters cover global conservation of amphibians, bats, birds and forests, conservation of European farmland biodiversity and some aspects of enhancing natural pest control, enhancing soil fertility and control of freshwater invasive species. It contains key results from the summarized evidence for each conservation intervention and an assessment of the effectiveness of each by international expert panels. The accompanying website www.conservationevidence.com describes each of the studies individually, and provides full references

What Works in Conservation: 2021 (PDF)

Does the creation of artificial reefs benefit subtidal benthic invertebrates? Is the use of organic farming instead of conventional farming beneficial to bat conservation? Does installing wildlife warning reflectors along roads benefit mammal conservation? Does the installation of exclusion and/or escape devices on fishing nets benefit marine and freshwater mammal conservation? What Works in Conservation has been created to provide practitioners with answers to these and many other questions about practical conservation. This book provides an assessment of the effectiveness of 2526 conservation interventions based on summarized scientific evidence. The 2021 edition containssubstantial new material on bat conservation, terrestrial mammal conservation and marine and freshwater mammals, thus completing the evidence for all mammal species categories. Other chapters cover practical global conservation of primates, amphibians, bats, birds, forests, peatlands, subtidal benthic invertebrates, shrublands and heathlands, as well as the conservation of European farmland biodiversity and some aspects of enhancing natural pest control, enhancing soil fertility, management of captive animals and control of freshwater invasive species. It contains key results from the summarized evidence for each conservation intervention and an assessment of the effectiveness of each by international expert panels. The accompanying website www.conservationevidence.com describes each of the studies individually, and provides full references. This is the fourth author-approved edition of What Works in Conservation, which is revised on an annual basis

What Works in Conservation 2019

What Works in Conservation has been created to provide practitioners with answers to these and many other questions about practical conservation. This book provides an assessment of the effectiveness of 1389 conservation interventions based on summarized scientific evidence. The 2019 edition contains new material on bat conservation. Other chapters cover practical global conservation of primates, peatlands, shrublands and heathlands, management of captive animals as well as an extended chapter on control of freshwater invasive species, the global conservation of amphibians, bats, birds and forests, conservation of European farmland biodiversity and some aspects of enhancing natural pest control, enhancing soil fertility and control of freshwater invasive species. It contains key results from the summarized evidence for each conservation intervention and an assessment of the effectiveness of each by international expert panels. The accompanying website www.conservationevidence.com describes each of the studies individually, and provides full references. This is the fourth edition of What Works in Conservation, which is revised on an annual basis. Arcadia, DEFRA, ESRC, MAVA Foundation, NERC, Natural England, Robert Bosch Stiftung, Synchronicity Earth, South West Water and Waitrose Ltd. have generously contributed towards the publication of this volume