A proteomic investigation of soluble olfactory proteins in Anopheles gambiae

Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are small soluble polypeptides that bind semiochemicals in the lymph of insect chemosensilla. In the genome of Anopheles gambiae, 66 genes encode OBPs and 8 encode CSPs. Here we monitored their expression through classical proteomics (2D gel-MS analysis) and a shotgun approach. The latter method proved much more sensitive and therefore more suitable for tiny biological samples as mosquitoes antennae and eggs. Females express a larger number and higher quantities of OBPs in their antennae than males (24 vs 19). OBP9 is the most abundant in the antennae of both sexes, as well as in larvae, pupae and eggs. Of the 8 CSPs, 4 were detected in antennae, while SAP3 was the only one expressed in larvae. Our proteomic results are in fairly good agreement with data of RNA expression reported in the literature, except for OBP4 and OBP5, that we could not identify in our analysis, nor could we detect in Western Blot experiments. The relatively limited number of soluble olfactory proteins expressed at relatively high levels in mosquitoes makes further studies on the coding of chemical messages at the OBP level more accessible, providing for few specific targets. Identification of such proteins in Anopheles gambiae might facilitate future studies on host finding behavior in this important disease vector. © 2013 Mastrobuoni et al

Linking jet emission and X-ray properties in the peculiar neutron star X-ray binary Circinus X-1

We present the results of simultaneous X-ray and radio observations of the peculiar Z-type neutron star X-ray binary Cir X-1, observed with the Rossi X-ray timing explorer satellite and the Australia Telescope Compact Array in 2000 October and 2002 December. We identify typical Z source behaviour in the power density spectra as well as characteristic Z patterns drawn in an X-ray hardness-intensity diagram. Power spectra typical of bright atoll sources have also been identified at orbital phases after the periastron passage, while orbital phases before the periastron passage are characterized by power spectra that are typical neither of Z nor of atoll sources. We investigate the coupling between the X-ray and the radio properties, focusing on three orbital phases when an enhancement of the radio flux density has been detected, to test the link between the inflow (X-ray) and the outflow (radio jet) to/from the compact object. In two out of three cases we associate the presence of the radio jet to a spectral transition in the X-rays, although the transition does not precede the radio flare, as detected in other Z sources. An analogous behaviour has recently been found in the black hole candidate GX 339-4. In the third case, the radio light curve shows a similar shape to the X-ray light curve. We discuss our results in the context of jet models, considering also black hole candidates.Comment: Accepted for publication in MNRA

Validation of PARADISE 24 and Development of PARADISE-EDEN 36 in Patients with Dementia

Dementia was one of the conditions focused on in an EU (European Union) project called “PARADISE” (Psychosocial fActors Relevant to brAin DISorders in Europe) that later produced a measure called PARADISE 24, developed within the biopsychosocial model proposed in the International Classification of Functioning Disability and Health (ICF). The aims of this study are to validate PARADISE 24 on a wider sample of patients with mild to moderate dementia to expand PARADISE 24 by defining a more specific scale for dementia, by adding 18 questions specifically selected for dementia, which eventually should be reduced to 12. We enrolled 123 persons with dementia, recruited between July 2017 and July 2019 in home care and long-term care facilities, in Italy, and 80 participants were recruited in Warsaw between January and July 2012 as part of a previous cross-sectional study. The interviews with the patient and/or family were conducted by health professionals alone or as a team by using the Paradise data collection protocol. The psychometric analysis with the Rasch analysis has shown that PARADISE 24 and the selection of 18 additional condition-specific items can be expected to have good measurement properties to assess the functional state in persons with dementia

Planck intermediate results XIV : Dust emission at millimetre wavelengths in the Galactic plane

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Planck intermediate results XIII : Constraints on peculiar velocities

Peer reviewe