Using zeta-potential measurements to quantify peptide partition to lipid membranes

© The Author(s) 2011. This article is published with open access at Springerlink.com.Open Access: This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.Many cellular phenomena occur on the biomembranes. There are plenty of molecules (natural or xenobiotics) that interact directly or partially with the cell membrane. Biomolecules, such as several peptides (e.g., antimicrobial peptides) and proteins, exert their effects at the cell membrane level. This feature makes necessary investigating their interactions with lipids to clarify their mechanisms of action and side effects necessary. The determination of molecular lipid/water partition constants (Kp) is frequently used to quantify the extension of the interaction. The determination of this parameter has been achieved by using different methodologies, such as UV-Vis absorption spectrophotometry, fluorescence spectroscopy and ζ-potential measurements. In this work, we derived and tested a mathematical model to determine the Kp from ζ-potential data. The values obtained with this method were compared with those obtained by fluorescence spectroscopy, which is a regular technique used to quantify the interaction of intrinsically fluorescent peptides with selected biomembrane model systems. Two antimicrobial peptides (BP100 and pepR) were evaluated by this new method. The results obtained by this new methodology show that ζ-potential is a powerful technique to quantify peptide/lipid interactions of a wide variety of charged molecules, overcoming some of the limitations inherent to other techniques, such as the need for fluorescent labeling.This work was partially supported by project PTDC/QUI/ 69937/2006 from Fundação para a Ciência e Tecnologia-Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal), and by Fundação Calouste Gulbenkian (Portugal). JMF and MMD also thank FCT-MCTES for grants IMM/BT/37-2010 and SFRH/BD/41750/2007, respectively

Checkpoints are blind to replication restart and recombination intermediates that result in gross chromosomal rearrangements

Replication fork inactivation can be overcome by homologous recombination, but this can cause gross chromosomal rearrangements that subsequently missegregate at mitosis, driving further chromosome instability. It is unclear when the chromosome rearrangements are generated and whether individual replication problems or the resulting recombination intermediates delay the cell cycle. Here we have investigated checkpoint activation during HR-dependent replication restart using a site-specific replication fork-arrest system. Analysis during a single cell cycle shows that HR-dependent replication intermediates arise in S phase, shortly after replication arrest, and are resolved into acentric and dicentric chromosomes in G2. Despite this, cells progress into mitosis without delay. Neither the DNA damage nor the intra-S phase checkpoints are activated in the first cell cycle, demonstrating that these checkpoints are blind to replication and recombination intermediates as well as to rearranged chromosomes. The dicentrics form anaphase bridges that subsequently break, inducing checkpoint activation in the second cell cycle

Equation-Oriented Modeling and Optimization of a Biorefinery Based on Avocado Waste

Due to the growth in the consumption of avocado in recent years, the amount of waste caused by avocado peel and seed has increased. Avocado waste can be transformed into valuable products such as energy, biofuels, and biological products using integrated processes in a biorefinery. This paper considers the detailed modeling, simulation, and optimization of a biorefinery for the production of phenolic compounds, bioethanol, biological xylitol, syngas, and electrical power from avocado seed and peel, using Aspen Plus in equation-oriented mode as a process simulation tool. For a biorefinery in nominal conditions, it is possible to achieve a gross profit of approximately 30x106 USD/year for capital costs of USD 31.4x106, while the combined effect of process optimization and heat integration allows reaching a gross profit of 37x106 USD/year for capital costs of USD 30.7x106. These results of the optimized plant show its potential to take advantage of avocado seed and peel in a profitable and sustainable way. This detailed equation-based model paves the way for superstructure optimization of a biorefinery for avocado waste processing

Scalable hardware architecture for disparity map computation and object location in real-time

We present the disparity map computation core of a hardware system for isolating foreground objects in stereoscopic video streams. The operation is based on the computation of dense disparity maps using block-matching algorithms and two well-known metrics: sum of absolute differences and Census transform. Two sets of disparity maps are computed by taking each of the images as reference so that a consistency check can be performed to identify occluded pixels and eliminate spurious foreground pixels. Taking advantage of parallelism, the proposed architecture is highly scalable and provides numerous degrees of adjustment to different application needs, performance levels and resource usage. A version of the system for 640 x 480 images and a maximum disparity of 135 pixels was implemented in a system based on a Xilinx Virtex II-Pro FPGA and two cameras with a frame rate of 25 fps (less than the maximum supported frame rate of 40 fps on this platform). Implementation of the same system on a Virtex-5 FPGA is estimated to achieve 80 fps, while a version with increased parallelism is estimated to run at 140 fps (which corresponds to the calculation of more than 5.9 x 10(9) disparity-pixels per second)

Predictions of the electro-mechanical response of conductive CNT-polymer composites

We present finite element simulations to predict the conductivity, elastic response and strain-sensing capability of conductive composites comprising a polymeric matrix and carbon nanotubes. Realistic representative volume elements (RVE) of the microstructure are generated and both constituents are modelled as linear elastic solids, with resistivity independent of strain; the electrical contact between nanotubes is represented by a new element which accounts for quantum tunnelling effects and captures the sensitivity of conductivity to separation. Monte Carlo simulations are conducted and the sensitivity of the predictions to RVE size is explored. Predictions of modulus and conductivity are found in good agreement with published results. The strain-sensing capability of the material is explored for multiaxial strain states

Sense of personal agency in adolescence and young adulthood: A preliminary assessment model

Although there is a growing interest in the concept of sense of personal agency in adolescence and young adulthood, its operationalization and assessment have been inconsistent. We propose and test a preliminary assessment model of sense of agency combining four of the most relevant indicators suggested by the literature for its assessment (setting goals, optimism, decision-making, and self-efficacy). We conducted three independent studies with young adults [study 1 = 692; study 2 = 410] and adolescents [study 3 = 481] to analyze its psychometric properties. The CFA results revealed a good fit to the data in all three studies. The results of studies 1 and 2 indicate that even though the four dimensions share a significant proportion of variance, they do not assess the overlapping aspects of sense of agency. The findings of study 1 clarified that the proposed measurement model is invariant across sex and different levels of psychosocial risk. The results of study 2 suggest that, as expected, the latent construct of sense of agency is linked to different dimensions of psycho-emotional adjustment of young adults. Lastly, the findings of study 3 revealed that our preliminary model is invariant across three assessment points establishing the measurement longitudinal invariance

Stability and Change in Adolescents’ Sense of Agency: Contributions of Sex, Multiple Risk, Pandemic Stress, and Attachment to Parents

Although literature states that individual, relational, and contextual factors contribute to adolescents’ sense of agency, more research is needed to clarify and understand how adolescents develop this belief over time. The current study examined the stability/change trajectories of the sense of agency during adolescence, specifically across high school, analyzing whether attachment to parents over time, adolescents’ sex, cumulative risk in baseline, and pandemic-related stress explained these trajectories. The sample included 467 Portuguese adolescents (40.7% were males; Mage = 15.58 years, SD = 0.80), evaluated three times across 18 months. This work yielded three significant findings. First, adolescents’ sense of agency significantly increased over time, with significant between-subject variance at the initial levels but not at the growth rate. Second, attachment to parents consistently links to adolescents’ sense of agency across time, despite the differential contributions from attachment to mothers and fathers. Third, boys reported greater growth in the sense of agency than girls. Adolescents’ cumulative risk at T1 predicted lower initial levels of sense of agency, whereas higher pandemic-related stress predicted less growth of the sense of agency. These findings emphasize the contributions of individual and family characteristics and the role of the broader social context in shaping the development of adolescents’ sense of agency. The findings underline the need to consider further the differential influences of adolescents’ relationships with mothers and fathers to understand changes in adolescents’ sense of agency

Digital subtraction radiographic analysis of the combination of bioabsorbable membrane and bovine morphogenetic protein pool in human periodontal infrabony defects

Objectives: This study assessed the bone density gain and its relationship with the periodontal clinical parameters in a case series of a regenerative therapy procedure. Material and Methods: Using a split-mouth study design, 10 pairs of infrabony defects from 15 patients were treated with a pool of bovine bone morphogenetic proteins associated with collagen membrane (test sites) or collagen membrane only (control sites). The periodontal healing was clinically and radiographically monitored for six months. Standardized presurgical and 6-month postoperative radiographs were digitized for digital subtraction analysis, which showed relative bone density gain in both groups of 0.034 ± 0.423 and 0.105 ± 0.423 in the test and control group, respectively (p>0.05). Results: As regards the area size of bone density change, the influence of the therapy was detected in 2.5 mm2 in the test group and 2 mm2 in the control group (p>0.05). Additionally, no correlation was observed between the favorable clinical results and the bone density gain measured by digital subtraction radiography (p>0.05). Conclusions: The findings of this study suggest that the clinical benefit of the regenerative therapy observed did not come with significant bone density gains. Long-term evaluation may lead to a different conclusions

An antidamping spin–orbit torque originating from the Berry curvature

Magnetization switching at the interface between ferromagnetic and paramagnetic metals, controlled by current-induced torques, could be exploited in magnetic memory technologies. Compelling questions arise regarding the role played in the switching by the spin Hall effect in the paramagnet and by the spin–orbit torque originating from the broken inversion symmetry at the interface. Of particular importance are the antidamping components of these current-induced torques acting against the equilibrium-restoring Gilbert damping of the magnetization dynamics. Here, we report the observation of an antidamping spin–orbit torque that stems from the Berry curvature, in analogy to the origin of the intrinsic spin Hall effect. We chose the ferromagnetic semiconductor (Ga,Mn)As as a material system because its crystal inversion asymmetry allows us to measure bare ferromagnetic films, rather than ferromagnetic paramagnetic heterostructures,eliminating by design any spin Hall effect contribution. We provide an intuitive picture of the Berry curvature origin of this antidamping spin–orbit torque as well as its microscopic modelling. We expect the Berry curvature spin–orbit torque to be of comparable strength to the spin-Hall effect-driven antidamping torque in ferromagnets interfaced with paramagnets with strong intrinsic spin Hall effect