Exploring the anthelmintic properties of Australian native shrubs with respect to their potential role in livestock grazing systems

We measured in vitro anthelmintic activity in extracts from 85 species of Australian native shrub, with a view to identifying species able to provide a degree of worm control in grazing systems. Approximately 40% of the species showed significant activity in inhibiting development of Haemonchus contortus larvae. The most active extracts showed IC50 values of 60–300 mg/ml. Pre-incubation with polyvinylpolypyrrolidine removed the activity from some extracts, implicating tannins as the bioactive agent, while in other cases the pre-incubation had no effect, indicating the presence of other anthelmintic compounds. Plant reproductive maturity (onset of flowering or fruiting) was associated with increasing anthelmintic activity in some species. Variability was observed between plants of the same species growing in different environments, while variation between individual plants of the same species within a single field suggests the existence of distinct chemotypes. Significant activity against adult H. contortus worms in vitro was also demonstrated in a limited number of extracts tested against this life stage. Our study indicates that there is potential for Australian native shrubs to play an anthelmintic role in grazing systems, and highlights some plant biology factors which will need to be considered in order to maximize any anthelmintic effects.A. C. Kotze, J. O’Grady, J. Emms, A. F. Toovey, S. Hughes, P. Jessop, M. Bennell P. E. Vercoe and D. K. Revel

Bioactive ceramic-reinforced composites for bone augmentation

Biomaterials have been used to repair the human body for millennia, but it is only since the 1970s that man-made composites have been used. Hydroxyapatite (HA)-reinforced polyethylene (PE) is the first of the ‘second-generation’ biomaterials that have been developed to be bioactive rather than bioinert. The mechanical properties have been characterized using quasi-static, fatigue, creep and fracture toughness testing, and these studies have allowed optimization of the production method. The in vitro and in vivo biological properties have been investigated with a range of filler content and have shown that the presence of sufficient bioactive filler leads to a bioactive composite. Finally, the material has been applied clinically, initially in the orbital floor and later in the middle ear. From this initial combination of HA in PE other bioactive ceramic polymer composites have been developed

Race and sex: teachers' views on who gets ahead in schools?

The research reported here was part of a large study of the impact of age, disability, race and sex on the teaching profession in England. The basic question asked in this research was how do these factors interact with career aspirations and achievements of classteachers, promoted teachers and headteachers? There were three different data sources: a large postal survey drawn from diverse geographic regions across England with over 2000 respondents; face‐to‐face individual interviews with over 100 teachers in 18 case study schools from across all of the main regions of England; discussions with special interest groups of teachers. Not surprisingly, the answer to the above question was complex. Nonetheless, the paper's conclusion highlights some of the noteworthy themes across this broad sample of teachers from primary, secondary and special schools

Lymphoid aggregates that resemble tertiary lymphoid organs define a specific pathological subset in metal-on-metal hip replacements

Aseptic lymphocyte-dominated vasculitis-associated lesion (ALVAL) has been used to describe the histological lesion associated with metal-on-metal (M-M) bearings. We tested the hypothesis that the lymphoid aggregates, associated with ALVAL lesions resemble tertiary lymphoid organs (TLOs). Histopathological changes were examined in the periprosthetic tissue of 62 M-M hip replacements requiring revision surgery, with particular emphasis on the characteristics and pattern of the lymphocytic infiltrate. Immunofluorescence and immunohistochemistry were used to study the classical features of TLOs in cases where large organized lymphoid follicles were present. Synchrotron X-ray fluorescence (XRF) measurements were undertaken to detect localisation of implant derived ions/particles within the samples. Based on type of lymphocytic infiltrates, three different categories were recognised; diffuse aggregates (51%), T cell aggregates (20%), and organised lymphoid aggregates (29%). Further investigation of tissues with organised lymphoid aggregates showed that these tissues recapitulate many of the features of TLOs with T cells and B cells organised into discrete areas, the presence of follicular dendritic cells, acquisition of high endothelial venule like phenotype by blood vessels, expression of lymphoid chemokines and the presence of plasma cells. Co-localisation of implant-derived metals with lymphoid aggregates was observed. These findings suggest that in addition to the well described general foreign body reaction mediated by macrophages and a T cell mediated type IV hypersensitivity response, an under-recognized immunological reaction to metal wear debris involving B cells and the formation of tertiary lymphoid organs occurs in a distinct subset of patients with M-M implants

The Introduction of the Prevent Duty into Schools and Colleges: Stories of Continuity and Change

Drawing on mixed methods research carried out with school and college staff during 2015 and 2016, this chapter provides insight into how the Prevent Duty ‘landed’ in schools and colleges during the first 18 months after its introduction in July 2015. The discussion centres on four key questions: (1) To what extent did staff express overall opposition to or support for the Prevent Duty? (2) To what extent was the Prevent Duty interpreted by staff in schools and colleges as a straightforward extension of existing safeguarding responsibilities? (3) To what extent did staff perceive the Duty to be exacerbating the stigmatisation of Muslim students? (4) To what extent did staff perceive the Duty to have a ‘chilling effect’ on classrooms and on student voices

Noroviruses as a Cause of Diarrhea in Immunocompromised Pediatric Hematopoietic Stem Cell and Solid Organ Transplant Recipients

Case reports describe significant norovirus gastroenteritis morbidity in immunocompromised patients. We evaluated norovirus pathogenesis in prospectively enrolled solid organ (SOT) and hematopoietic stem cell transplant (HSCT) patients with diarrhea who presented to Texas Children\u27s Hospital and submitted stool for enteric testing. Noroviruses were detected by real-time reverse transcription polymerase chain reaction. Clinical outcomes of norovirus diarrhea and non-norovirus diarrhea patients, matched by transplanted organ type, were compared. Norovirus infection was identified in 25 (22%) of 116 patients, more frequently than other enteropathogens. Fifty percent of norovirus patients experienced diarrhea lasting ≥14 days, with median duration of 12.5 days (range 1–324 days); 29% developed diarrhea recurrence. Fifty-five percent of norovirus patients were hospitalized for diarrhea, with 27% requiring intensive care unit (ICU) admission. One HSCT recipient developed pneumatosis intestinalis. Three HSCT patients expired ≤6 months of norovirus diarrhea onset. Compared to non-norovirus diarrhea patients, norovirus patients experienced significantly more frequent ICU admission (27% vs. 0%, p = 0.02), greater serum creatinine rise (median 0.3 vs. 0.2 mg/dL, p = 0.01), and more weight loss (median 1.6 vs. 0.6 kg, p \u3c 0.01). Noroviruses are an important cause of diarrhea in pediatric transplant patients and are associated with significant clinical complications

A Climate Index Optimized for Longshore Sediment Transport Reveals Interannual and Multidecadal Littoral Cell Rotations

A recent 35-year endpoint shoreline change analysis revealed significant counterclockwiserotations occurring in north-central Oregon, USA, littoral cells that extend 10s of kilometers in length.While the potential for severe El Niños to contribute to littoral cell rotations at seasonal to interannual scalewas previously recognized, the dynamics resulting in persistent (multidecadal) rotation were unknown,largely due to a lack of historical wave conditions extending back multiple decades and the difficulty ofseparating the timescales of shoreline variability in a high energy region. This study addresses this questionby (1) developing a statistical downscaling framework to characterize wave conditions relevant for longshoresediment transport during data-poor decades and (2) applying a one-line shoreline change model toquantitatively assess the potential for such large embayed beaches to rotate. A climateINdex was optimizedto capture variability in longshore wave power as a proxy for potentialLOngshore Sediment Transport(LOST_IN), and a procedure was developed to simulate many realizations of potential wave conditions fromthe index. Waves were transformed dynamically with Simulating Waves Nearshore to the nearshore asinputs to a one-line model that revealed shoreline rotations of embayed beaches at multiple time and spatialscales not previously discernible from infrequent observations. Model results indicate that littoral cellsrespond to both interannual and multidecadal oscillations, producing comparable shoreline excursions toextreme El Niño winters. The technique quantitatively relates morphodynamic forcing to specific climatepatterns and has the potential to better identify and quantify coastal variability on timescales relevant to achanging climate.This work would not have been possible without funding from the NSF Graduate Research Fellowship Program (GRFP) through NSF grant DGE-1314109, the Coastal and Ocean Climate Applications (COCA) program through NOAA grant NA15OAR4310243, NOAA’s Regional Integrated Sciences and Assessments Program (RISA), under NOAA grant NA15OAR4310145, and the Spanish Ministerio de Educación Cultura y Deporte FPU (Formación del Profesorado Universitario) studentship BOE-A-2013-12235. Beach survey data collection undertaken on the Oregon coast was made possible by the Northwest Association of Networked Ocean Observing Systems (NANOOS) through NOAA grant NA16NOS0120019

Ozone sensitivity to varying greenhouse gases and ozone-depleting substances in CCMI-1 simulations

Ozone fields simulated for the first phase of the Chemistry-Climate Model Initiative (CCMI-1) will be used as forcing data in the 6th Coupled Model Intercomparison Project. Here we assess, using reference and sensitivity simulations produced for CCMI-1, the suitability of CCMI-1 model results for this process, investigating the degree of consistency amongst models regarding their responses to variations in individual forcings. We consider the influences of methane, nitrous oxide, a combination of chlorinated or brominated ozone-depleting substances, and a combination of carbon dioxide and other greenhouse gases. We find varying degrees of consistency in the models' responses in ozone to these individual forcings, including some considerable disagreement. In particular, the response of total-column ozone to these forcings is less consistent across the multi-model ensemble than profile comparisons. We analyse how stratospheric age of air, a commonly used diagnostic of stratospheric transport, responds to the forcings. For this diagnostic we find some salient differences in model behaviour, which may explain some of the findings for ozone. The findings imply that the ozone fields derived from CCMI-1 are subject to considerable uncertainties regarding the impacts of these anthropogenic forcings. We offer some thoughts on how to best approach the problem of generating a consensus ozone database from a multi-model ensemble such as CCMI-1

The effect of atmospheric nudging on the stratospheric residual circulation in chemistry–climate models

We perform the first multi-model intercomparison of the impact of nudged meteorology on the stratospheric residual circulation using hindcast simulations from the Chemistry–Climate Model Initiative (CCMI). We examine simulations over the period 1980–2009 from seven models in which the meteorological fields are nudged towards a reanalysis dataset and compare these with their equivalent free-running simulations and the reanalyses themselves. We show that for the current implementations, nudging meteorology does not constrain the mean strength of the stratospheric residual circulation and that the inter-model spread is similar, or even larger, than in the free-running simulations. The nudged models generally show slightly stronger upwelling in the tropical lower stratosphere compared to the free-running versions and exhibit marked differences compared to the directly estimated residual circulation from the reanalysis dataset they are nudged towards. Downward control calculations applied to the nudged simulations reveal substantial differences between the climatological lower-stratospheric tropical upward mass flux (TUMF) computed from the modelled wave forcing and that calculated directly from the residual circulation. This explicitly shows that nudging decouples the wave forcing and the residual circulation so that the divergence of the angular momentum flux due to the mean motion is not balanced by eddy motions, as would typically be expected in the time mean. Overall, nudging meteorological fields leads to increased inter-model spread for most of the measures of the mean climatological stratospheric residual circulation assessed in this study. In contrast, the nudged simulations show a high degree of consistency in the inter-annual variability in the TUMF in the lower stratosphere, which is primarily related to the contribution to variability from the resolved wave forcing. The more consistent inter-annual variability in TUMF in the nudged models also compares more closely with the variability found in the reanalyses, particularly in boreal winter. We apply a multiple linear regression (MLR) model to separate the drivers of inter-annual and long-term variations in the simulated TUMF; this explains up to ∼75 % of the variance in TUMF in the nudged simulations. The MLR model reveals a statistically significant positive trend in TUMF for most models over the period 1980–2009. The TUMF trend magnitude is generally larger in the nudged models compared to their free-running counterparts, but the intermodel range of trends doubles from around a factor of 2 to a factor of 4 due to nudging. Furthermore, the nudged models generally do not match the TUMF trends in the reanalysis they are nudged towards for trends over different periods in the interval 1980–2009. Hence, we conclude that nudging does not strongly constrain long-term trends simulated by the chemistry–climate model (CCM) in the residual circulation. Our findings show that while nudged simulations may, by construction, produce accurate temperatures and realistic representations of fast horizontal transport, this is not typically the case for the slower zonal mean vertical transport in the stratosphere. Consequently, caution is required when using nudged simulations to interpret the behaviour of stratospheric tracers that are affected by the residual circulation

Validating Operator Event Sequence Diagrams: The case of an automated vehicle to human driver handovers

Abstract: Predicting what drivers will do as vehicle control is handed over to them from automation is a relatively new challenge for the motor vehicle industry. Operator Event Sequence Diagrams (OESDs) offer a way of modeling the interactions between the driver and vehicle automation in the handover of control. In this paper, two studies are presented in which a range of handover strategies are tested. The anticipated driver strategies were modeled using OESDs to serve as predictions of driver behavior. Drivers were then observed in two separate studies: (1) using a Lower‐Fidelity (vehicle seat and controls) simulator and (2) using a Higher‐Fidelity (whole vehicle) simulator. Driver behavior during a takeover task was categorized according to the signal detection paradigm into hits, misses, false alarms, and correct rejections. The results showed that for all strategies in both sets of studies, the median criterion for validity was exceeded ( φ > 0.8), suggesting that OESDs made good predictions of driver behavior during the handover of the vehicle from automation to manual control