Intraguild predation between lady beetles and lacewings: outcomes and consequences vary with focal prey and arena of interaction

Citation: Noppe, Christophe, J. P. Michaud, and Patrick De Clercq. 2012. “Intraguild Predation Between Lady Beetles and Lacewings: Outcomes and Consequences Vary With Focal Prey and Arena of Interaction.” Annals of the Entomological Society of America 105 (4): 562–71. https://doi.org/10.1603/AN11165.We examined reciprocal intraguild predation (IGP) and cannibalism among various combinations of Coleomegilla maculata DeGeer and Chrysoperla carnea Stephens larvae as they developed feeding on greenbugs, Schizaphis graminum Rondani, on sorghum plants in microcosms. Pairs of C. maculata larvae suppressed aphids better than pairs of C. carnea larvae or heterospecific larval pairs and yielded the highest rate of plant survival. IGP by C. carnea larvae occurred mostly in the first instar, whereas C. maculata larvae were more aggressive in later instars. Although C. carnea was the superior intraguild predator, winning 62.7% of contests in microcosms, this value increased to 88.9% when the experiment was repeated in petri dishes without plant material, regardless of whether greenbugs or eggs of Ephestia kuehniella Zeller were offered as focal prey. Provision in petri dishes of the sessile, higher quality prey (Ephestia) as opposed to greenbugs, improved the survival of solitary larvae and delayed cannibalism and IGP until later developmental stages in both species. Larvae of C. maculata that cannibalized took longer to develop and weighed less at pupation, independent of the arena or prey offered. Although larvae of C. carnea did not pay a cost for cannibalism or IGP in microcosms, there were some negative developmental effects of IGP in petri dishes, particularly on the Ephestia diet. These results illustrate how the plant, as a substrate, can mediate the strength of IGP interactions and how the relative suitability of the focal prey can influence both the timing and consequences of cannibalism and IGP

Glocalisation

Deze bundel brengt verslag uit van de tweedaagse conferentie die de Stichting Maatschappij en Veiligheid uit Nederland (SMV) en het Centrum voor Politiestudies uit België (CPS) organiseerden op 12 en 13 december 2013 in Deinze. De conferentie had als thema "Tides and currents in police theories", waarbij het de bedoeling was te reflecteren over hoe er tegenwoordig wordt gedacht over politie, met andere woorden over de onderliggende stromen en evoluties in politietheorieën. Rond ditzelfde thema werd tevens een Cahier Politiestudies samengesteld dat op de tweedaagse conferentie aan de deelnemers en sprekers werd aangeboden3. Het thema van de conferentie en het Cahier was gebaseerd op een paper van Jack Greene "The Tides and Currents, Eddies and Whirlpools and Riptides of Modern Policing: Connecting Thoughts". Deze paper werd geschreven naar aanleiding van een seminarie dat plaatsvond bij de Universiteit Gent in het kader van de werkgroep "politie" van de European Society of Criminology (ESC) in september 2010. Met deze conferentie wilden we de analyse verder doortrekken en kwamen naast de theoretische inzichten ook de beleidsmatige implicaties ervan aan bod. Met deze publicatie willen we de rijke discussies die zich ontwikkelden ter gelegenheid van deze conferentie onder de aandacht van de geïnteresseerden brengen en continuëren. De publicatie is deels in het Nederlands, deels in het Engels, waarbij we de auteurs vrij lieten de taal van hun voorkeur te hanteren.The politics and administration of institutional chang

Occurrence of pharmaceuticals in environmental samples: a multi-analyte approach

Pharmaceuticals and their residues in the environment have been recently recognized as one of the emerging research areas in the environmental chemistry and toxicology and caused them to be viewed as a new class of priority substances. It has been reportedthat they are introduced continuously in the environment via household use, effluents from Sewage Treatment Plants (STPs) and animal excreta. Up to date, the potential human, animal and ecological risk associated with the occurrence of these compounds in the environment is not well documented. There is an increased attention due to the fact that they are designed to have specific effects at low doses and to be resistant to Moreover, the science of mixture toxicity is complex and to date quite unknown (Bound et al., 2006; Hernando et al., 2006).Despite the increased research and regulatory interest in the occurrence of pharmaceuticals and their degradation products in STPs effluents and freshwater ecosystems (Hernando et al., 2006), the occurrence, the distribution between the different environmental compartments (i.e. water, sediments, suspended solids and aqueous organisms), the trophic transfer and their potential toxicity is to date far less documented (Emblidge and DeLorenze, 2006).In this sense, this study will present a detection method for the determination of a large group of pharmaceuticals (i.e. antibiotics, beta-agonists, painkillers, tranquilizers, nonsteroidal anti-inflammatory drugs) used both in human and veterinary practice in environmental samples using Liquid Chromatography coupled to multiple Mass Spectrometry

Contact inhibition of locomotion and mechanical cross-talk between cell-cell and cell-substrate adhesion determines the pattern of junctional tension in epithelial cell aggregates

We generated a computational approach to analyze the biomechanics of epithelial cell aggregates, either island or stripes or entire monolayers, that combines both vertex and contact-inhibition-of-locomotion models to include both cell-cell and cell-substrate adhesion. Examination of the distribution of cell protrusions (adhesion to the substrate) in the model predicted high order profiles of cell organization that agree with those previously seen experimentally. Cells acquired an asymmetric distribution of basal protrusions, traction forces and apical aspect ratios that decreased when moving from the edge to the island center. Our in silico analysis also showed that tension on cell-cell junctions and apical stress is not homogeneous across the island. Instead, these parameters are higher at the island center and scales up with island size, which we confirmed experimentally using laser ablation assays and immunofluorescence. Without formally being a 3-dimensional model, our approach has the minimal elements necessary to reproduce the distribution of cellular forces and mechanical crosstalk as well as distribution of principal stress in cells within epithelial cell aggregates. By making experimental testable predictions, our approach would benefit the mechanical analysis of epithelial tissues, especially when local changes in cell-cell and/or cell-substrate adhesion drive collective cell behavior.Comment: 39 pages, 8 Figures. Supplementary Information is include

Endocrine disruption in the Scheldt estuary distribution, exposure and effects (ENDIS-RISKS). Final report

ENDIS-RISKS is a multidisciplinary, research project conducted by five institutes. This project aimed to assess the distribution, exposure and effects of endocrine disruptors in the Scheldt estuary, with specific attention to invertebrates. The Scheldt estuary is known to be one of the most polluted estuaries in the world. The industrial areas of Ghent and Antwerp are to a large extent responsible for this pollution. To achieve these goals detailed knowledge of the distribution and long-term effects of these substances is needed. This information is crucial for the development of future-oriented policy measures at the national and European level. The project can be divided into four different research phases. In Phase I the occurance and distribution of endocrine disrupting substances in the Scheldt estuary was studied. Water, sediment, suspended solids and biota were sampled 3 times a year for a period of 4 years (2002-2006). In all these matrices, 7 groups of chemicals were analysed: estrogens, pesticides, phthalates, organotins, polyaromatic components (PCBs, PBDEs), polyaromatic hydrocarbons (PAHs) and phenols. All the analyzed chemicals are on the OSPAR list of priority chemicals or are indicated as endocrine disruptors on this list. The different water samples were also tested using in vitro assays to assess their potential to bind to the (human) estrogen and androgen receptor. Phase II evaluated the exposure of biota occuring in the Scheldt estuary to endocrine disrupting substances. Based on the results of the chemical analysis, priority substances were selected. Phase III studied the effects of endocrine disrupting substances occurring in the Scheldt estuary on resident mysid shrimp populations (laboratory and field studies). Substances of concern were selected and tested in the laboratory to evaluate their effects on the estuarine mysid Neomysis integer. In the context of this project, three new assays using invertebrate-specific endpoints were developed to examine the effect of endocrine disrupting chemicals (EDCs) on molting, embryogenesis and vitellogenesis of N. integer. Finally, in Phase IV laboratory and field results were used to perform a preliminary environmental risk assessment of endocrine disruptors in the Scheldt estuary. Samples were collected along the salinity gradiënt of the Scheldt estuary with the RV Belgica. Water samples were taken with Teflon-coated Go-Flo bottles (10L), sediment samples with Van Veen Grab, biota with a hyperbentic sledge, and suspended particulate matter (SPM) was continuously sampled with an Alfa Laval flow-through centrifuge. For the chemical analysis, protocols were developed to analyse estrogens, organotriazine herbicides, organochlorine pesticides, phtalates, organotins, PAHs, PCBs, and PBDEs in the different matrices: i.e. water, sediment, SPM and biota.Experimental studies were performed to analyse growth, molting, embryogenesis and vitellogenesis of N. integer. These studies were needed to develop ecotoxicological assays to evaluate EDCs on these physiological processes. To study growth of N. integer, organisms were individually transferrred in exposure solutions and molts were collected to measure the growth after each molting. To study embryogenesis, embryos were taking out of the marsupium and placed in multiwell plates. Each day survival, developmental stages and hatching was analysed. To study vitellogenesis, vitellin was isolated from eggs with gelfitration and polyclonal antibodies were developed (in rabbits). With the isolated vitellin and the antibodies an enzyme-linked immunosorbent assay (ELISA) was developed. Vitellin was quatified in ovigerous females exposed to test compound in the laboratory and in females collected from the different sampling sites of the Scheldt estuary. In addition to vitellin levels, energy allocation and testosterone metabolism was examined in field collected mysids. Finally, results from population stu

Male-male marriage in Sinophone and Anglophone Harry Potter danmei and slash

The aim of this study is to compare Sinophone and Anglophone fan fiction consisting of female-oriented male-male romance: danmei and slash, respectively. To increase comparability, we analysed Harry Potter fan fiction in which the characters Harry and Draco are married. Male-male marriage was selected because our online Sinophone and Anglophone BL fandom surveys indicate this to be the most popular story element of the nine options we provided. We analysed five stories originally written in Chinese and five originally written in English which subsequently had been fan-translated into Chinese. Using Thematic Analysis (Braun & Clarke, 2006) we found some robust patterns. In contrast to the Anglophone fiction, the Sinophone tended to: stress the importance of family approval for the marriage; incorporate a wedding ceremony; employ clearly gendered roles between partners; utilise extended, as opposed to nuclear, families; and showed the couple to produce children, particularly boys. Hence, the stories mirror the relative social conservatism and social liberalism of their cultures of origin. However, in reading and writing such danmei young Chinese women are still pushing at the boundaries of the traditional family

Hadron Energy Reconstruction for the ATLAS Calorimetry in the Framework of the Non-parametrical Method

This paper discusses hadron energy reconstruction for the ATLAS barrel prototype combined calorimeter (consisting of a lead-liquid argon electromagnetic part and an iron-scintillator hadronic part) in the framework of the non-parametrical method. The non-parametrical method utilizes only the known $e/h$ ratios and the electron calibration constants and does not require the determination of any parameters by a minimization technique. Thus, this technique lends itself to an easy use in a first level trigger. The reconstructed mean values of the hadron energies are within $\pm 1$ of the true values and the fractional energy resolution is $[(58\pm3)/\sqrt{E}+(2.5\pm0.3)$. The value of the $e/h$ ratio obtained for the electromagnetic compartment of the combined calorimeter is $1.74\pm0.04$ and agrees with the prediction that $e/h > 1.7$ for this electromagnetic calorimeter. Results of a study of the longitudinal hadronic shower development are also presented. The data have been taken in the H8 beam line of the CERN SPS using pions of energies from 10 to 300 GeV.Comment: 33 pages, 13 figures, Will be published in NIM

Observation of the Early Structural Changes Leading to the Formation of Protein Superstructures.

Formation of superstructures in protein aggregation processes has been indicated as a general pathway for several proteins, possibly playing a role in human pathologies. There is a severe lack of knowledge on the origin of such species in terms of both mechanisms of formation and structural features. We use equine lysozyme as a model protein, and by combining spectroscopic techniques and microscopy with X-ray fiber diffraction and ab initio modeling of Small Angle X-ray Scattering data, we isolate the partially unfolded state from which one of these superstructures (i.e., particulate) originates. We reveal the low-resolution structure of the unfolded state and its mechanism of formation, highlighting the physicochemical features and the possible pathway of formation of the particulate structure. Our findings provide a novel detailed knowledge of such a general and alternative aggregation pathway for proteins, this being crucial for a basic and broader understanding of the aggregation phenomena.This is the author's accepted manuscript and will be under embargo until the 3rd of September 2015. The final version is published by ACS in The Journal of Physical Chemistry Letters here: http://pubs.acs.org/doi/abs/10.1021/jz501614e