Eesti Evangeelse Luterliku Kiriku Viljandi praostkonna kirikumuusikud koostöös paikkonna muusika- ja kultuurieluga aastatel 2000-2010

Käesoleva uurimistöö eesmärgiks oli välja selgitada kirikumuusikute ja paikkonna vahelist muusika- ja kultuurialast koostööd soodustavad ning takistavad tegurid.http://tartu.ester.ee/record=b2608714~S13*es

Small molecule probes of protein aggregation

Understanding the mechanisms of amyloid formation and toxicity remain major challenges. Whilst substantial progress has been made in the development of methods able to identify the species formed during self-assembly and to describe the kinetic mechanisms of aggregation, the structure(s) of non-native species, including potentially toxic oligomers, remain elusive. Moreover, how fibrils contribute to disease remains unclear. Here we review recent advances in the development of small molecules and other reagents that are helping to define the mechanisms of protein aggregation in molecular detail. Such probes form a powerful platform with which to better define the mechanisms of structural conversion into amyloid fibrils and may provide the much-needed stepping stone for future development of successful therapeutic agents

Narva. Vaade Hermanni kindlusele (linnusele) O-st

PostkaartTekst foto tagaküljel: Narva. Die Hermannsfestung von der Ostseite. Trükitud: Karl Noormägi. Tempel: Instituu

Narva. Kaubasadam, paremal bastion "Pax", 20. sajandi algus

PostkaartTekst foto tagaküljel: Narva. Die Festung Ivangorod. Trükitud: Karl Noormägi broom-piltpostkaartide töökoda Trallinnas. - Reval, Estonie. Tempel: Instituu

Dynamics of soil respiration in storm areas-Sõõru storm damaged areas as an example

Käesoleva töö raames uuritakse mullahingamist tormikahjustatud aladel häiringujärgselt. Töö eesmärgiks on teada saada faktorid, mis mõjutavad mullahingamist häiringujärgselt tormikahjustatud alal ja kas mullahingamine on erinevalt kahjustatud aladel erinev. Töö käigus koguti mullahingamise ja mulla temperatuuri andmeid. Seejärel teostati statsistiline analüüs. Andmeanalüüsi käigus leiti, et mullahingamist mõjutavad proovitüki asukoht, kahjustatus, mõõtmispäev ja mõõtmisala iseloom. Mullatemperatuur ei mõjutanud oluliselt mullahingamist. Mullahingamised olid sarnased koristatud alal ja täielikult kahjustatud alal, kus tormi tagajärjel oli hävinud kogu puurinne. Mullahingamised olid erinevad täielikult kahjustatud alal ja osaliselt kahjustatud alal, kus tormiga oli hävinud ca 50% puurindest. Mullahingamised olid erinevad ka osaliselt kahjustatud alal ja koristatud alal.The aim of the study was to examine the soil respiration in storm damaged areas after windthrow. The work aims to find out what are the factors affecting soil respiration after windthrow in storm damaged area and whether soil respiration is affected by disturbance severity (totally disturbed, partially disturbed) and actions taken place after disturbance (area harvested after disturbance, areas not harvested after disturbance). During this work the soil respiration and soil temperature data was collected and statistical analysis were performed. I found that soil respiration is affected by the location of the plot, damage severity and measuring day. Soil temperature did not significantly affect soil respiration. Soil respirations were similar in harvested areas and totally damaged areas where the storm had destroyed the tree layer. Soil respirations were different in totally damaged and partially damaged areas where the storm had destroyed about 50% of tree layer. Soil respirations were different in partially damaged areas and harvested areas

Evaluation of Zn2+- and Cu2+-Binding Affinities of Native Cu,Zn-SOD1 and Its G93A Mutant by LC-ICP MS

The tight binding of Cu and Zn ions to superoxide dismutase 1 (SOD1) maintains the protein stability, associated with amyotrophic lateral sclerosis (ALS). Yet, the quantitative studies remain to be explored for the metal-binding affinity of wild-type SOD1 and its mutants. We have investigated the demetallation of Cu,Zn-SOD1 and its ALS-related G93A mutant in the presence of different standard metal ion chelators at varying temperatures by using an LC-ICP MS-based approach and fast size-exclusion chromatography. Our results showed that from the slow first-order kinetics both metal ions Zn2+ and Cu2+ were released simultaneously from the protein at elevated temperatures. The rate of the release depends on the concentration of chelating ligands but is almost independent of their metal-binding affinities. Similar studies with the G93A mutant of Cu,Zn-SOD1 revealed slightly faster metal-release. The demetallation of Cu,Zn-SOD1 comes always to completion, which hindered the calculation of the KD values. From the Arrhenius plots of the demetallation in the absence of chelators ΔH‡ = 173 kJ/mol for wt and 191 kJ/mol for G93A mutant Cu,Zn-SOD1 was estimated. Obtained high ΔH values are indicative of the occurrence of protein conformational changes before demetallation and we concluded that Cu,Zn-SOD1 complex is in native conditions kinetically inert. The fibrillization of both forms of SOD1 was similar