Development of RAPD, DAMD and ISSR markers for authentication of medicinal plant Cassia auriculata and its adulterant Cassia surattensis

Cassia auriculata is an important traditional medicinal plant commonly used in many Ayurvedic formulations, meant for diabetes, rheumatism, conjunctivitis, infertility and etc. But due to similar morphological characters and misidentifications, adulteration from Cassia surattensis has been observed. Since safety and efficacy of herbal products has become a major concern due to adulterations, DNA profiling which is an effective and cheap method can be used to solve this problem by discriminating the genuine material. However, no reports about the genetic identification for these species are available to our knowledge. Therefore, the present study was devoted to developing Random Amplified Polymorphic DNA (RAPD), Direct Amplification of Minisatellite-region DNA (DAMD) and Inter Simple Sequence Repeat (ISSR) DNA profiles to authenticate C. auriculata and C. surattensis. As the first step, fresh leaf samples from both plant species were randomly collected from Gampaha district, Sri Lanka and genomic DNA were extracted using modified Cetyltrimethylammonium Bromide (CTAB) protocols. Four short arbitrary primers, two core primers and two SSR primers were used respectively with three different Polymerase Chain Reactions (PCR) based molecular markers which were RAPD, DAMD and ISSR to develop DNA profiles. Out of eight primers, three arbitrary primers, OP-F03, OP-U10 and OP-U20 and one core primer, HBV(5) yielded clear and reproducible amplification products. These results clearly discriminated the medicinal plant C. auriculata and the adulterant C. surattensis providing a complementary tool for quality control of plant derived herbal medicinal products. However, both SSR primers couldn't authenticate two plants and further work is needed to develop ISSR DNA profiles for the authentication

Isolation and Characterization of Plant growth-promoting Endophyticdiazotrophic Bacteria from Sri Lankan Rice Cultivars and Rapid Screening for their effect on Plant Growth Promotion

The present study was conducted to isolate and identify endophyticdiazotrophic bacteria in two Sri Lankan rice (Oryza Sativa L.) varieties; Suwandel and Bg 358 and to evaluate their potential to promote rice plant growth. A total of 15 putative endophyticdiazotrophic bacterial isolates were obtained from shoots and roots of Suwandel and Bg 358 rice varieties out of which 7 isolates were selected based on their ability to produce IAA and phosphate solubilization. According to the morphological characters and biochemical tests, these bacteria were identified belong to genera Bacillus (IN003, IN006, and IN007), Klebsiella (IN008 and IN018), Pantoea (IN009), and Enterobacter (IN015). All selected bacterial isolates produced IAA (7.1µmgl-1 to 30.9 µ mgl-1) in the tryptophan supplemented medium. Five out of seven bacterial isolates (IN006, IN007, IN008, IN015, and IN018) were able to solubilize inorganic phosphate on Pikovskaya\u27s agar medium. Rice seeds (Suwandel variety) treated with these endophyticdiazotrophic bacteria with plant growth-promoting ability showed significantly enhanced shoot length, root length, shoot fresh weight, shoot dry weight and root fresh weight compared to the uninoculated control. Plant inoculation experiment indicated that Enterobacter sp. (IN015) was most effective in rice plant growth promotion among seven bacterial isolates tested. These results strongly suggest that endophyticdiazotrophic bacteria characterized in this study could be successfully used to promote rice plant growth

The Rest-Frame Submillimeter Spectrum of High-Redshift, Dusty, Star-Forming Galaxies

We present the average rest-frame spectrum of high-redshift dusty, star-forming galaxies from 250-770GHz. This spectrum was constructed by stacking ALMA 3mm spectra of 22 such sources discovered by the South Pole Telescope and spanning z=2.0-5.7. In addition to multiple bright spectral features of 12CO, [CI], and H2O, we also detect several faint transitions of 13CO, HCN, HNC, HCO+, and CN, and use the observed line strengths to characterize the typical properties of the interstellar medium of these high-redshift starburst galaxies. We find that the 13CO brightness in these objects is comparable to that of the only other z>2 star-forming galaxy in which 13CO has been observed. We show that the emission from the high-critical density molecules HCN, HNC, HCO+, and CN is consistent with a warm, dense medium with T_kin ~ 55K and n_H2 >~ 10^5.5 cm^-3. High molecular hydrogen densities are required to reproduce the observed line ratios, and we demonstrate that alternatives to purely collisional excitation are unlikely to be significant for the bulk of these systems. We quantify the average emission from several species with no individually detected transitions, and find emission from the hydride CH and the linear molecule CCH for the first time at high redshift, indicating that these molecules may be powerful probes of interstellar chemistry in high-redshift systems. These observations represent the first constraints on many molecular species with rest-frame transitions from 0.4-1.2mm in star-forming systems at high redshift, and will be invaluable in making effective use of ALMA in full science operations.Comment: 19 pages, 10 figures (2 in appendices); accepted for publication in Ap

The redshift distribution of dusty star forming galaxies from the SPT survey

We use the Atacama Large Millimeter/submillimeter Array (ALMA) in Cycle 1 to determine spectroscopic redshifts of high-redshift dusty star-forming galaxies (DSFGs) selected by their 1.4mm continuum emission in the South Pole Telescope (SPT) survey. We present ALMA 3mm spectral scans between 84-114GHz for 15 galaxies and targeted ALMA 1mm observations for an additional eight sources. Our observations yield 30 new line detections from CO, [CI] , [NII] , H_2O and NH_3. We further present APEX [CII] and CO mid-J observations for seven sources for which only a single line was detected in spectral-scan data from ALMA Cycle 0 or Cycle 1. We combine the new observations with previously published and new mm/submm line and photometric data of the SPT-selected DSFGs to study their redshift distribution. The combined data yield 39 spectroscopic redshifts from molecular lines, a success rate of >85%. Our sample represents the largest data set of its kind today and has the highest spectroscopic completeness among all redshift surveys of high-z DSFGs. The median of the redshift distribution is z=3.9+/-0.4, and the highest-redshift source in our sample is at z=5.8. We discuss how the selection of our sources affects the redshift distribution, focusing on source brightness, selection wavelength, and strong gravitational lensing. We correct for the effect of gravitational lensing and find the redshift distribution for 1.4mm-selected sources with a median redshift of z=3.1+/-0.3. Comparing to redshift distributions selected at shorter wavelengths from the literature, we show that selection wavelength affects the shape of the redshift distribution

SPT 0538-50: Physical conditions in the ISM of a strongly lensed dusty star-forming galaxy at z=2.8

We present observations of SPT-S J053816-5030.8, a gravitationally-lensed dusty star forming galaxy (DSFG) at z = 2.7817, first discovered at millimeter wavelengths by the South Pole Telescope. SPT 0538-50 is typical of the brightest sources found by wide-field millimeter-wavelength surveys, being lensed by an intervening galaxy at moderate redshift (in this instance, at z = 0.441). We present a wide array of multi-wavelength spectroscopic and photometric data on SPT 0538-50, including data from ALMA, Herschel PACS and SPIRE, Hubble, Spitzer, VLT, ATCA, APEX, and the SMA. We use high resolution imaging from HST to de-blend SPT 0538-50, separating DSFG emission from that of the foreground lens. Combined with a source model derived from ALMA imaging (which suggests a magnification factor of 21 +/- 4), we derive the intrinsic properties of SPT 0538-50, including the stellar mass, far-IR luminosity, star formation rate, molecular gas mass, and - using molecular line fluxes - the excitation conditions within the ISM. The derived physical properties argue that we are witnessing compact, merger-driven star formation in SPT 0538-50, similar to local starburst galaxies, and unlike that seen in some other DSFGs at this epoch.Comment: 16 pages, 11 figures. Accepted for publication in Ap

Anatomy of a post-starburst minor merger: a multi-wavelength WFC3 study of NGC 4150

(Abridged) We present a spatially-resolved near-UV/optical study of NGC 4150, using the Wide Field Camera 3 (WFC3) on board the Hubble Space Telescope. Previous studies of this early-type galaxy (ETG) indicate that it has a large reservoir of molecular gas, exhibits a kinematically decoupled core (likely indication of recent merging) and strong, central H_B absorption (indicative of young stars). The core of NGC 4150 shows ubiquitous near-UV emission and remarkable dusty substructure. Our analysis shows this galaxy to lie in the near-UV green valley, and its pixel-by-pixel photometry exhibits a narrow range of near-UV/optical colours that are similar to those of nearby E+A (post-starburst) galaxies. We parametrise the properties of the recent star formation (age, mass fraction, metallicity and internal dust content) in the NGC 4150 pixels by comparing the observed near-UV/optical photometry to stellar models. The typical age of the recent star formation (RSF) is around 0.9 Gyrs, consistent with the similarity of the near-UV colours to post-starburst systems, while the morphological structure of the young component supports the proposed merger scenario. The RSF metallicity, representative of the metallicity of the gas fuelling star formation, is around 0.3 - 0.5 Zsun. Assuming that this galaxy is a merger and that the gas is sourced mainly from the infalling companion, these metallicities plausibly indicate the gas-phase metallicity (GPM) of the accreted satellite. Comparison to the local mass-GPM relation suggests (crudely) that the mass of the accreted system is around 3x10^8 Msun, making NGC 4150 a 1:20 minor merger. A summation of the pixel RSF mass fractions indicates that the RSF contributes about 2-3 percent of the stellar mass. This work reaffirms our hypothesis that minor mergers play a significant role in the evolution of ETGs at late epochs.Comment: 28 pages, 2 tables, accepted for publication in Ap

Planck Intermediate Results. IV. The XMM-Newton validation programme for new Planck galaxy clusters

We present the final results from the XMM-Newton validation follow-up of new Planck galaxy cluster candidates. We observed 15 new candidates, detected with signal-to-noise ratios between 4.0 and 6.1 in the 15.5-month nominal Planck survey. The candidates were selected using ancillary data flags derived from the ROSAT All Sky Survey (RASS) and Digitized Sky Survey all-sky maps, with the aim of pushing into the low SZ flux, high-z regime and testing RASS flags as indicators of candidate reliability. 14 new clusters were detected by XMM, including 2 double systems. Redshifts lie in the range 0.2 to 0.9, with 6 clusters at z>0.5. Estimated M500 range from 2.5 10^14 to 8 10^14 Msun. We discuss our results in the context of the full XMM validation programme, in which 51 new clusters have been detected. This includes 4 double and 2 triple systems, some of which are chance projections on the sky of clusters at different z. We find that association with a RASS-BSC source is a robust indicator of the reliability of a candidate, whereas association with a FSC source does not guarantee that the SZ candidate is a bona fide cluster. Nevertheless, most Planck clusters appear in RASS maps, with a significance greater than 2 sigma being a good indication that the candidate is a real cluster. The full sample gives a Planck sensitivity threshold of Y500 ~ 4 10^-4 arcmin^2, with indication for Malmquist bias in the YX-Y500 relation below this level. The corresponding mass threshold depends on z. Systems with M500 > 5 10^14 Msun at z > 0.5 are easily detectable with Planck. The newly-detected clusters follow the YX-Y500 relation derived from X-ray selected samples. Compared to X-ray selected clusters, the new SZ clusters have a lower X-ray luminosity on average for their mass. There is no indication of departure from standard self-similar evolution in the X-ray versus SZ scaling properties. (abridged)Comment: accepted by A&