36 research outputs found
The seasonal nitrogen cycle in Wilkinson Basin, Gulf of Maine, as estimated by 1-D biological model optimization
Author Posting. Š Elsevier B.V., 2009. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Journal of Marine Systems 78 (2009): 77-93, doi:10.1016/j.jmarsys.2009.04.001.The objective of this study was to fit a simple ecosystem model to climatological
nitrogen cycle data in the Gulf of Maine, in order to calibrate the biological model
for use in future 3-D modelling studies. First depth-dependent monthly climatologies
of nitrate, ammonium, chlorophyll, zooplankton, detritus and primary production
data from Wilkinson Basin, Gulf of Maine, were created. A 6-box nitrogen-based
ecosystem model was objectively fitted to the data through parameter optimization.
Optimization was based on weighted least squares with model-data misfits nondi-
mensionalized by assigned uncertainties in the monthly climatological estimates.
These uncertainties were estimated as the standard deviations of the raw data from
the 6-meter and monthly bin averages. On average the model fits the monthly means
almost within their assigned uncertainties.
Several statistics are examined to assess model-data misfit. Pattern statistics such
as the correlation coefficient lack practical significance when data errors are large
relative to the signal, as in this application. Thus Taylor diagrams were not found
to be useful. The RMSE and model bias normalized by the data error were found
to be the most useful skill metrics as they indicate whether the model fits the data
within its estimated error.
The optimal simulated nitrogen cycle budgets are presented, as an estimate of the
seasonal nitrogen cycle in Wilkinson Basin, and discussed in context of the available
data.Wilkinson Basin has spring and fall phytoplankton blooms, and strong summer
stratification with a deep chlorophyll maximum near 21 m, just above the nitracline.
The mean euphotic zone depth is estimated to be 25 m, relatively constant with
season. The model estimates annual primary production as 176 g C mâ2 yrâ1,
annual new production as 71 g C mâ2 yrâ1 and sinking PON fluxes of 9.7 and 4.7
g N mâ2 yrâ1 at 24 and 198 m respectively.
Areas for improvement in the biological model, the model optimization method,
and significant data gaps are identified.This work was supported by ONR, NSF, and NOAA grant to Dennis
McGillicuddy
Gain-of-function screen for genes that affect Drosophila muscle pattern formation.
This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation
Zusammenfassung
N-Heterocyclic carbenes (NHCs) have been shown to be useful ligands for the Suzuki-Miyaura cross-coupling at low catalyst loadings. We now report that the commercially available and air-stable [Pd(IPr)(cin)Cl] pre-catalyst permits the formation of various functionalized biaryls from aryl chlorides and boronic acids (37 examples) under very mild conditions using a mixture of ethanol/water as solvent and an inorganic base
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Morphophysiology of potato (Solanum tuberosum) in response to drought stress: paving the way forward
The cultivated potato (Solanum tuberosum L.) is currently the third most important food crop in the world and is becoming increasingly important to the local economies of developing countries. Climate change threatens to drastically reduce potato yields in areas of the world where the growing season is predicted to become hotter and drier. Modern potato is well known as an extremely drought susceptible crop, which has primarily been attributed to its shallow root system. This review addresses this decades old consensus, and highlights other, less well understood, morphophysiological features of potato which likely contribute to drought susceptibility. This review explores the effects of drought on these traits and goes on to discuss phenotypes which may be associated with drought tolerance in potato. Small canopies which increase harvest index and decrease evapotranspiration, open stem-type canopies which increase light penetration, and shallow but densely rooted cultivars, which increase water uptake, have all been associated with drought tolerance in the past, but have largely been ignored. While individual studies on a limited number of cultivars may have examined these phenotypes, they are typically overlooked due to the consensus that root depth is the only significant cause of drought susceptibility in potato. We review this work, particularly with respect to potato morphology, in the context of a changing climate, and highlight the gaps in our understanding of drought tolerance in potato that such work implies