‘Smart Cities’ – Dynamic Sustainability Issues and Challenges for ‘Old World’ Economies: A Case from the United Kingdom

The rapid and dynamic rate of urbanization, particularly in emerging world economies, has resulted in a need to find sustainable ways of dealing with the excessive strains and pressures that come to bear on existing infrastructures and relationships. Increasingly during the twenty-first century policy makers have turned to technological solutions to deal with this challenge and the dynamics inherent within it. This move towards the utilization of technology to underpin infrastructure has led to the emergence of the term ‘Smart City’. Smart cities incorporate technology based solutions in their planning development and operation. This paper explores the organizational issues and challenges facing a post-industrial agglomeration in the North West of England as it attempted to become a ‘Smart City’. In particular the paper identifies and discusses the factors that posed significant challenges for the dynamic relationships residents, policymakers and public and private sector organizations and as a result aims to use these micro-level issues to inform the macro-debate and context of wider Smart City discussions. In order to achieve this, the paper develops a range of recommendations that are designed to inform Smart City design, planning and implementation strategies

Gymnosperms on the EDGE

Driven by limited resources and a sense of urgency, the prioritization of species for conservation has been a persistent concern in conservation science. Gymnosperms (comprising ginkgo, conifers, cycads, and gnetophytes) are one of the most threatened groups of living organisms, with 40% of the species at high risk of extinction, about twice as many as the most recent estimates for all plants (i.e. 21.4%). This high proportion of species facing extinction highlights the urgent action required to secure their future through an objective prioritization approach. The Evolutionary Distinct and Globally Endangered (EDGE) method rapidly ranks species based on their evolutionary distinctiveness and the extinction risks they face. EDGE is applied to gymnosperms using a phylogenetic tree comprising DNA sequence data for 85% of gymnosperm species (923 out of 1090 species), to which the 167 missing species were added, and IUCN Red List assessments available for 92% of species. The effect of different extinction probability transformations and the handling of IUCN data deficient species on the resulting rankings is investigated. Although top entries in our ranking comprise species that were expected to score well (e.g. Wollemia nobilis, Ginkgo biloba), many were unexpected (e.g. Araucaria araucana). These results highlight the necessity of using approaches that integrate evolutionary information in conservation science

Well Excuse Me! Replicating and Connecting Excuse-Seeking Behaviors

Excuse-seeking behavior that facilitates replacing altruistic choices with self-interested ones has been documented in several domains. In a laboratory study, we replicate three leading papers on this topic: Dana et al. (2007), and the use of information avoidance; Exley (2015), and the use of differential risk preferences; and Di Tella et al. (2015), and the use of motivated beliefs. The replications were conducted as part of a graduate course, attempting to embed one answer to the growing call for experimental replications within the pedagogic process. We fully replicate the simpler Dana et al. paper, and broadly replicate the core findings for the other two projects, though with reduced effect sizes and a failure to replicate on some secondary measures. Finally, we attempt to connect behaviors to facilitate the understanding of how each fit within the broader literature. However, we find no connections across domains

The Late Stages of Protoplanetary Disk Evolution: A Millimeter Survey of Upper Scorpius

We present deep 1.2 millimeter photometry of 37 stars in the young (5 Myr) Upper Scorpius OB association, sensitive to ~4 x 10^-3 Mjup of cool millimeter dust. Disks around four low- and solar-mass stars are detected, as well as one debris disk around an intermediate mass star, with dust masses ranging from 3.6 x 10^-3 -- 1.0 x 10^-1 Mjup. The source with the most massive disk exhibits a transition-disk spectral energy distribution. Combining our results with previous studies, we find the millimeter-detection fraction of Class II sources has significantly decreased from younger ages, and comparison with near-infrared and Halpha measurements indicates the present disks have undergone significant evolution in composition or structure at all radii. The disks of Upper Scorpius represent the tail-end of the depletion of primordial disks; while a few near-solar mass stars may still sustain giant planet formation, this process has finished around higher mass starsComment: Accepted by ApJ. 8 pages, 5 figures, 4 table

Diseño y validación mediante la Teoría de Respuesta al Ítem del Instrumento para Evaluar Capital Psicológico en las Organizaciones IPSICAP

16 p.El constructo “capital psicológico”, creado por Fred Luthans, se define como un estado de desarrollo psicológico positivo del ser humano, que lo caracteriza por (a) tener confianza (autoeficacia) para realizar los esfuerzos que sean necesarios con el fin de alcanzar el éxito en tareas retadoras; (b) hacer atribuciones de causalidad positivas (optimismo) acerca de los sucesos presentes y futuros; (c) perseverar en el logro de los objetivos y, cuando sea necesario, redireccionar los caminos para alcanzarlos (esperanza) de manera exitosa; y (d) al ser blanco de los problemas y la adversidad, mantenerse en pie, volver a comenzar e ir más allá (resiliencia) para lograr el éxito (Luthans, Youssef & Avolio, 2007a, 2007b). Este constructo ha surgido a partir de investigación empírica dentro del comportamiento organizacional positivo, y se ha identificado como un factor nuclear (core factor) de segundo orden (Avey, Patera & West, 2006). Específicamente, las bases teóricas de sus cuatro componentes tienen origen en la psicología clínica, y la aplicación al contexto laboral ha sido realizada principalmente por Fred Luthans, Carolyn Youssef y Bruce Avolio (Luthans & Avolio, 2003; Luthans, Avolio, Walumbwa & Li, 2005); aunque también ha sido trabajado por el grupo de investigación WoNT-Work and Organizational Network, dirigido por Marisa Salanova, en España.Introducción Método Resultados Discusión Referencia

Multigene Molecular Systematics Confirm Species Status of Morphologically Convergent Pagurus Hermit Crabs

Introduction: In spite of contemporary morphological taxonomy appraisals, apparent high morphological similarity raises uncertainty about the species status of certain Pagurus hermit crabs. This is exemplified between two European species, Pagurus excavatus (Herbst, 1791) and Pagurus alatus (Fabricius 1775), whose species status is still difficult to resolve using morphological criteria alone. Methodology/Principal Findings: To address such ambiguities, we used combinations of Maximum Likelihood (ML) and Bayesian Inference (BI) methods to delineate species boundaries of P. alatus and P. excavatus and formulate an intermediate Pagurus phylogenetic hypothesis, based upon single and concatenated mitochondrial (cytochrome oxidase I [COI]) and nuclear (16S and 28s ribosomal RNA) gene partitions. The molecular data supported the species status of P. excavatus and P. alatus and also clearly resolved two divergent clades within hermit crabs from the Northeast Atlantic Ocean and the Mediterranean Sea. Conclusions/Significance: Despite the abundance and prominent ecological role of hermit crabs, Pagurus, in North East Atlantic Ocean and Mediterranean Sea ecosystems, many important aspects of their taxonomy, biology, systematics and evolution remain poorly explored. The topologies presented here should be regarded as hypotheses that can be incorporated into the robust and integrated understanding of the systematic relationships within and between species of the genus Pagurus inhabiting the Northeast Atlantic Ocean and the Mediterranean Sea

Optimization of the doxycycline-dependent simian immunodeficiency virus through in vitro evolution

<p>Abstract</p> <p>Background</p> <p>Vaccination of macaques with live attenuated simian immunodeficiency virus (SIV) provides significant protection against the wild-type virus. The use of a live attenuated human immunodeficiency virus (HIV) as AIDS vaccine in humans is however considered unsafe because of the risk that the attenuated virus may accumulate genetic changes during persistence and evolve to a pathogenic variant. We earlier presented a conditionally live HIV-1 variant that replicates exclusively in the presence of doxycycline (dox). Replication of this vaccine strain can be limited to the time that is needed to provide full protection through transient dox administration. Since the effectiveness and safety of such a conditionally live virus vaccine should be tested in macaques, we constructed a similar dox-dependent SIV variant. The Tat-TAR transcription control mechanism in this virus was inactivated through mutation and functionally replaced by the dox-inducible Tet-On regulatory system. This SIV-rtTA variant replicated in a dox-dependent manner in T cell lines, but not as efficiently as the parental SIVmac239 strain. Since macaque studies will likely require an efficiently replicating variant, we set out to optimize SIV-rtTA through in vitro viral evolution.</p> <p>Results</p> <p>Upon long-term culturing of SIV-rtTA, additional nucleotide substitutions were observed in TAR that affect the structure of this RNA element but that do not restore Tat binding. We demonstrate that the bulge and loop mutations that we had introduced in the TAR element of SIV-rtTA to inactivate the Tat-TAR mechanism, shifted the equilibrium between two alternative conformations of TAR. The additional TAR mutations observed in the evolved variants partially or completely restored this equilibrium, which suggests that the balance between the two TAR conformations is important for efficient viral replication. Moreover, SIV-rtTA acquired mutations in the U3 promoter region. We demonstrate that these TAR and U3 changes improve viral replication in T-cell lines and macaque peripheral blood mononuclear cells (PBMC) but do not affect dox-control.</p> <p>Conclusion</p> <p>The dox-dependent SIV-rtTA variant was optimized by viral evolution, yielding variants that can be used to test the conditionally live virus vaccine approach and as a tool in SIV biology studies and vaccine research.</p

Malignant hyperthermia

Malignant hyperthermia (MH) is a pharmacogenetic disorder of skeletal muscle that presents as a hypermetabolic response to potent volatile anesthetic gases such as halothane, sevoflurane, desflurane and the depolarizing muscle relaxant succinylcholine, and rarely, in humans, to stresses such as vigorous exercise and heat. The incidence of MH reactions ranges from 1:5,000 to 1:50,000–100,000 anesthesias. However, the prevalence of the genetic abnormalities may be as great as one in 3,000 individuals. MH affects humans, certain pig breeds, dogs, horses, and probably other animals. The classic signs of MH include hyperthermia to marked degree, tachycardia, tachypnea, increased carbon dioxide production, increased oxygen consumption, acidosis, muscle rigidity, and rhabdomyolysis, all related to a hypermetabolic response. The syndrome is likely to be fatal if untreated. Early recognition of the signs of MH, specifically elevation of end-expired carbon dioxide, provides the clinical diagnostic clues. In humans the syndrome is inherited in autosomal dominant pattern, while in pigs in autosomal recessive. The pathophysiologic changes of MH are due to uncontrolled rise of myoplasmic calcium, which activates biochemical processes related to muscle activation. Due to ATP depletion, the muscle membrane integrity is compromised leading to hyperkalemia and rhabdomyolysis. In most cases, the syndrome is caused by a defect in the ryanodine receptor. Over 90 mutations have been identified in the RYR-1 gene located on chromosome 19q13.1, and at least 25 are causal for MH. Diagnostic testing relies on assessing the in vitro contracture response of biopsied muscle to halothane, caffeine, and other drugs. Elucidation of the genetic changes has led to the introduction, on a limited basis so far, of genetic testing for susceptibility to MH. As the sensitivity of genetic testing increases, molecular genetics will be used for identifying those at risk with greater frequency. Dantrolene sodium is a specific antagonist of the pathophysiologic changes of MH and should be available wherever general anesthesia is administered. Thanks to the dramatic progress in understanding the clinical manifestation and pathophysiology of the syndrome, the mortality from MH has dropped from over 80% thirty years ago to less than 5%