A vision based aerial rbot solution for the IARC 2014 by the Technical University of Madrid

The IARC competitions aim at making the state of the art in UAV progress. The 2014 challenge deals mainly with GPS/Laser denied navigation, Robot-Robot interaction and Obstacle avoidance in the setting of a ground robot herding problem. We present in this paper a drone which will take part in this competition. The platform and hardware it is composed of and the software we designed are introduced. This software has three main components: the visual information acquisition, the mapping algorithm and the Aritificial Intelligence mission planner. A statement of the safety measures integrated in the drone and of our efforts to ensure field testing in conditions as close as possible to the challenge?s is also included

Using the Synergy between HPLC-MS and MALDI-MS Imaging to Explore the Lipidomics of Clear Cell Renal Cell Carcinoma

Lipid imaging mass spectrometry (LIMS) has been tested in several pathological contexts, demonstrating its ability to segregate and isolate lipid signatures in complex tissues, thanks to the technique’s spatial resolution. However, it cannot yet compete with the superior identification power of high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS), and therefore, very often, the latter is used to refine the assignment of the species detected by LIMS. Also, it is not clear if the differences in sensitivity and spatial resolution between the two techniques lead to a similar panel of biomarkers for a given disease. Here, we explore the capabilities of LIMS and HPLC-MS to produce a panel of lipid biomarkers to screen nephrectomy samples from 40 clear cell renal cell carcinoma patients. The same set of samples was explored by both techniques, and despite the important differences between them in terms of the number of detected and identified species (148 by LIMS and 344 by HPLC-MS in negative-ion mode) and the presence/absence of image capabilities, similar conclusions were reached: using the lipid fingerprint, it is possible to set up classifiers that correctly identify the samples as either healthy or tumor samples. The spatial resolution of LIMS enables extraction of additional information, such as the existence of necrotic areas or the existence of different tumor cell populations, but such information does not seem determinant for the correct classification of the samples, or it may be somehow compensated by the higher analytical power of HPLC-MS. Similar conclusions were reached with two very different techniques, validating their use for the discovery of lipid biomarkers.The work was funded by the Basque Government (IT971-16, IT1162-19, and ELKARTEK KK2018-00090) and has been developed as a Ph.D. project of LMS, who is the recipient of a Predoctoral Fellowship from the Spanish Government (BES- 2016-078721) . The authors are grateful to SGiker Lipidomic Service (UPV/EHU, MICINN, GV/EG, ESF) for the expert advice and technical and human support in MALDI and HPLC- MS analysis

J-PLUS:Searching for very metal-poor star candidates using the SPEEM pipeline

We explore the stellar content of the Javalambre Photometric Local Universe Survey (J-PLUS) Data Release 2 and show its potential to identify low-metallicity stars using the Stellar Parameters Estimation based on Ensemble Methods (SPEEM) pipeline. SPEEM is a tool to provide determinations of atmospheric parameters for stars and separate stellar sources from quasars, using the unique J-PLUS photometric system. The adoption of adequate selection criteria allows the identification of metal-poor star candidates suitable for spectroscopic follow-up. SPEEM consists of a series of machine learning models which uses a training sample observed by both J-PLUS and the SEGUE spectroscopic survey. The training sample has temperatures Teff between 4\,800 K and 9\,000 K; $\log g$ between 1.0 and 4.5, and $-3.

Primary and Secondary Immunodeficiency Diseases in Oncohaematology: Warning Signs, Diagnosis, and Management

Background: Immunodeficiencies (ID), in particular primary immunodeficiencies (PID), are often associated with haematological manifestations, such as peripheral cytopenias or lymphoproliferative syndromes. Early diagnosis and management have significant prognostic implications. Secondary immunodeficiencies (SID) may also be induced by oncohaematological diseases and their treatments. Haematologists and oncologists must therefore be aware of the association between blood disorders and cancer and ID, and be prepared to offer their patients appropriate treatment without delay. Our aim was to define the warning signs of primary and secondary IDs in paediatric and adult patients with oncohaematological manifestations.Methods: A multidisciplinary group of six experts (2 haematologists, 2 immunologists, and 2 paediatricians specializing in ID) conducted a literature review and prepared a document based on agreements reached an in-person meeting. An external group of 44 IDs specialists from all over Spain assessed the document and were consulted regarding their level of agreement.Results: This document identifies the haematological and extra-haematological diseases that should prompt a suspicion of PIDs in adults and children, in both primary care and haematology and oncology departments. Cytopenia and certain lymphoproliferative disorders are key diagnostic pointers. The diagnosis must be based on a detailed clinical history, physical exploration, complete blood count and standard laboratory tests. The immunological and haematological tests included in the diagnostic process will depend on the care level. Patients who are candidates for immunoglobulin replacement therapy must be carefully selected, and treatment should be offered as soon as possible to avoid the development of complications. Finally, this document recommends procedures for monitoring these patients.Conclusions: This document combines scientific evidence with the opinion of a broad panel of experts, and emphasizes the importance of an early diagnosis and treatment to avoid complications. The resulting document is a useful tool for primary care physicians and specialists who see both adult and paediatric patients with oncohaematological diseases

The wide-field, multiplexed, spectroscopic facility WEAVE : survey design, overview, and simulated implementation

Funding for the WEAVE facility has been provided by UKRI STFC, the University of Oxford, NOVA, NWO, Instituto de Astrofísica de Canarias (IAC), the Isaac Newton Group partners (STFC, NWO, and Spain, led by the IAC), INAF, CNRS-INSU, the Observatoire de Paris, Région Île-de-France, CONCYT through INAOE, Konkoly Observatory (CSFK), Max-Planck-Institut für Astronomie (MPIA Heidelberg), Lund University, the Leibniz Institute for Astrophysics Potsdam (AIP), the Swedish Research Council, the European Commission, and the University of Pennsylvania.WEAVE, the new wide-field, massively multiplexed spectroscopic survey facility for the William Herschel Telescope, will see first light in late 2022. WEAVE comprises a new 2-degree field-of-view prime-focus corrector system, a nearly 1000-multiplex fibre positioner, 20 individually deployable 'mini' integral field units (IFUs), and a single large IFU. These fibre systems feed a dual-beam spectrograph covering the wavelength range 366-959 nm at R ∼ 5000, or two shorter ranges at R ∼ 20,000. After summarising the design and implementation of WEAVE and its data systems, we present the organisation, science drivers and design of a five- to seven-year programme of eight individual surveys to: (i) study our Galaxy's origins by completing Gaia's phase-space information, providing metallicities to its limiting magnitude for ∼ 3 million stars and detailed abundances for ∼ 1.5 million brighter field and open-cluster stars; (ii) survey ∼ 0.4 million Galactic-plane OBA stars, young stellar objects and nearby gas to understand the evolution of young stars and their environments; (iii) perform an extensive spectral survey of white dwarfs; (iv) survey  ∼ 400 neutral-hydrogen-selected galaxies with the IFUs; (v) study properties and kinematics of stellar populations and ionised gas in z 1 million spectra of LOFAR-selected radio sources; (viii) trace structures using intergalactic/circumgalactic gas at z > 2. Finally, we describe the WEAVE Operational Rehearsals using the WEAVE Simulator.PostprintPeer reviewe

The wide-field, multiplexed, spectroscopic facility WEAVE: Survey design, overview, and simulated implementation

WEAVE, the new wide-field, massively multiplexed spectroscopic survey facility for the William Herschel Telescope, will see first light in late 2022. WEAVE comprises a new 2-degree field-of-view prime-focus corrector system, a nearly 1000-multiplex fibre positioner, 20 individually deployable 'mini' integral field units (IFUs), and a single large IFU. These fibre systems feed a dual-beam spectrograph covering the wavelength range 366$-$959\,nm at $R\sim5000$, or two shorter ranges at $R\sim20\,000$. After summarising the design and implementation of WEAVE and its data systems, we present the organisation, science drivers and design of a five- to seven-year programme of eight individual surveys to: (i) study our Galaxy's origins by completing Gaia's phase-space information, providing metallicities to its limiting magnitude for $\sim$3 million stars and detailed abundances for $\sim1.5$ million brighter field and open-cluster stars; (ii) survey $\sim0.4$ million Galactic-plane OBA stars, young stellar objects and nearby gas to understand the evolution of young stars and their environments; (iii) perform an extensive spectral survey of white dwarfs; (iv) survey $\sim400$ neutral-hydrogen-selected galaxies with the IFUs; (v) study properties and kinematics of stellar populations and ionised gas in $z<0.5$ cluster galaxies; (vi) survey stellar populations and kinematics in $\sim25\,000$ field galaxies at $0.3\lesssim z \lesssim 0.7$; (vii) study the cosmic evolution of accretion and star formation using $>1$ million spectra of LOFAR-selected radio sources; (viii) trace structures using intergalactic/circumgalactic gas at $z>2$. Finally, we describe the WEAVE Operational Rehearsals using the WEAVE Simulator.Comment: 41 pages, 27 figures, accepted for publication by MNRA

The wide-field, multiplexed, spectroscopic facility WEAVE: Survey design, overview, and simulated implementation

WEAVE, the new wide-field, massively multiplexed spectroscopic survey facility for the William Herschel Telescope, will see first light in late 2022. WEAVE comprises a new 2-degree field-of-view prime-focus corrector system, a nearly 1000-multiplex fibre positioner, 20 individually deployable 'mini' integral field units (IFUs), and a single large IFU. These fibre systems feed a dual-beam spectrograph covering the wavelength range 366−959\,nm at R∼5000, or two shorter ranges at R∼20000. After summarising the design and implementation of WEAVE and its data systems, we present the organisation, science drivers and design of a five- to seven-year programme of eight individual surveys to: (i) study our Galaxy's origins by completing Gaia's phase-space information, providing metallicities to its limiting magnitude for ∼3 million stars and detailed abundances for ∼1.5 million brighter field and open-cluster stars; (ii) survey ∼0.4 million Galactic-plane OBA stars, young stellar objects and nearby gas to understand the evolution of young stars and their environments; (iii) perform an extensive spectral survey of white dwarfs; (iv) survey ∼400 neutral-hydrogen-selected galaxies with the IFUs; (v) study properties and kinematics of stellar populations and ionised gas in z1 million spectra of LOFAR-selected radio sources; (viii) trace structures using intergalactic/circumgalactic gas at z>2. Finally, we describe the WEAVE Operational Rehearsals using the WEAVE Simulator

Revisión femoral con tallo cónico de fijación distal.

BackgroundProximal bone loss at the femoral stem is the main challenge facing surgeons at the time of revisión total hip arthroplasty. The aim of revision surgeries to obtain a stable placement of components and to restore joint kinematics. ObjectivesThe purpose of this paper is to present our experience in hip revisions with tapered distal stem fixation with a minimum follow-up of 2 years. Evaluate the survival of the implants used, Harris hip score, complications and reinterventions associated with the use of these stems for complex femoral revision surgeries.Study Design & MethodsSeries of Cases. We evaluated 37 patients (39 hips) between 2010 and 2014 in which non-cemented femoral stems were used (28 modular and 11 non-modular).The average age was 63.5 years (SD 13.3). Study patients included 18 (48.64%) women and 19 (51.35%) men. Causes of reoperation included 14 (35.89%) cases of aseptic loosening, 14 (35.89%) infections, 7 (17.94%) periprosthetic fractures, 3 (7.69%) stem fracture and 1 (2.56%) for instability. Mean follow-up was 42 months (range, 24-74 months).ResultsThe survival of the femoral stem for any reason according to Kaplan-Meier's analysis was 92.3%. The average Harris score improved from 37 (10-77) points before the operation to 81 points (33-96) at last follow-up. Four patients (10,2 %) presented dislocation; one (2,5 %) deep infection; one (2,5 %) had an intra-operative fracture; subsidence took place in 3 (7,6 %) patients and it was necessary to reurse 2 (5,1 %) femoral implants.ConclusionsThe femoral stem has showed satisfactory results in 2 to 6 years of follow-up in several conditions of femoral revision surgeries. It requires a simple technique with clinical improvement that has been widely published in the literature.IntroducciónLa pérdida de hueso proximal en la cirugía de revisión del vástago femoral es el principal desafío que presenta el cirujano al momento de realizar una revisión. El objetivo es obtener una fijación estable de los componentes, así como la restauración de la cinemática de la articulación.Material y métodosEvaluamos 37 pacientes (39 revisiones femorales) entre 2010 y 2014 en los que se utilizó tallos cónicos no cementado de fijación distal (28 modular y 11 no modulares). La edad promedio fue 63,5 años (DS: 13,3). Fueron incluidos 18 (48,64%) mujeres y 19 (51,35%) hombres. Se intervinieron 14 (35,89%) casos de aflojamiento aséptico, 14 (35,89%) infecciones, 7 (17,94%) fracturas peri protésicas, 3 (7,69%) fractura de vástago y 1 (2,56%) por inestabilidad.  El seguimiento medio fue de 42 meses (rango, 24-74).ResultadosLa puntuación de Harris media mejoró de 37 (10-77) puntos antes de la operación a 81 (33-96) puntos en el último seguimiento. Cuatro pacientes (10,2%) presentaron luxación; uno (2,5%) infección profunda; uno (2,5%) tuvo una fractura intra-operatoria; se produjo hundimiento en 3 (7,6%) pacientes y  fue necesario revisar 2 (5,1%)  implantes femorales.ConclusionesEl vástago cónico ha mostrado resultados satisfactorios en 2 a 6 años de seguimiento en varias condiciones de revisión femoral. Emplea una técnica simple y reproducible con una mejoría clínica ampliamente publicada

Combining imaging mass spectrometry and immunohistochemistry to analyse the lipidome of spinal cord inflammation

Inflammation is a complex process that accompanies many pathologies. Actually, dysregulation of the inflammatory process is behind many autoimmune diseases. Thus, treatment of such pathologies may benefit from in-depth knowledge of the metabolic changes associated with inflammation. Here, we developed a strategy to characterize the lipid fingerprint of inflammation in a mouse model of spinal cord injury. Using lipid imaging mass spectrometry (LIMS), we scanned spinal cord sections from nine animals injected with lysophosphatidylcholine, a chemical model of demyelination. The lesions were demonstrated to be highly heterogeneous, and therefore, comparison with immunofluorescence experiments carried out in the same section scanned by LIMS was required to accurately identify the morphology of the lesion. Following this protocol, three main areas were defined: the lesion core, the peri-lesion, which is the front of the lesion and is rich in infiltrating cells, and the uninvolved tissue. Segmentation of the LIMS experiments allowed us to isolate the lipid fingerprint of each area in a precise way, as demonstrated by the analysis using classification models. A clear difference in lipid signature was observed between the lesion front and the epicentre, where the damage was maximized. This study is a first step to unravel the changes in the lipidome associated with inflammation in the context of diverse pathologies, such as multiple sclerosis.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This project was supported by grants from the Basque Government (IT1491-22, IT1551-22) and from MCIN/AEI/https://doi.org/10.13039/501100011033 and by “ERDF A way of making Europe” (PID2019-109724RB-I00, PID2021-127918NB-I00 and PID2022-1382760B-100). IC and AM thank the Basque Government and the Spanish Ministry of Education and Science for their predoctoral fellowships. The authors acknowledge the technical support and mass spectrometry resources provided by SGIker (UPV/EHU) as well as the microscope facilities provided by SGIker (UPV/EHU) and the Achucarro Basque Center for Neuroscience

MALDI-IMS files of 53 experiments over sections of nevus, primary melanoma and melanoma metastasis in imzml format

&lt;p&gt;MALDI-IMS files of 53 experiments over sections of nevus, primary melanoma and melanoma metastasis in imzml format. The experiments were recorded in negative-ion mode with a MALDI LTQ-orbitrap XL (ThermoFisher) at 25 um/pixel of spatial resolution. We also uploaded three .ppt with a comparison between the eosin-hematoxilin images, the IHC (MelanA and HMB45) and the segmentation images&lt;/p&gt