The Role of Mammalian Reservoir Hosts in Tick-Borne Flavivirus Biology

Small-to-medium sized mammals and large animals are lucrative sources of blood meals for ixodid ticks that transmit life-threatening tick-borne flaviviruses (TBFVs). TBFVs have been isolated from various organs obtained from wild-caught Myodes and Apodemus species in Europe and Asia. Thus, these rodents are well-established reservoirs of TBFVs. Wild-caught Peromyscus species have demonstrated seropositivity against Powassan virus, the only TBFV known to circulate in North America, suggesting that they may play an important role in the biology of the virus in this geographic region. However, virus isolation from Peromyscus species is yet to be demonstrated. Wild-caught medium-sized mammals, such as woodchucks (Marmota monax) and skunks (Mephitis mephitis) have also demonstrated seropositivity against POWV, and virus was isolated from apparently healthy animals. Despite the well-established knowledge that small-to-medium sized animals are TBFV reservoirs, specific molecular biology addressing host-pathogen interactions remains poorly understood. Elucidating these interactions will be critical for gaining insight into the mechanism(s) of viral pathogenesis and/or resistance

Are Swift Long-Lag Gamma-Ray Bursts in the Local Supercluster?

A sample of 18 long-lag (tau_{lag} > 1 s) Gamma-Ray Bursts (GRBs) has been drawn from our catalog of all Swift long GRBs. Four different tests are done on this sample to test the prediction that a large fraction of long-lag GRBs are from our Local Supercluster. The results of these four tests come out that: (1) the distribution of these GRBs shows no tendency towards the Supergalactic plane; (2) the distribution shows no tendency towards the Virgo or Coma Cluster; (3) no associated bright host galaxies (m <=15) in the Local Supercluster are found for any of the 18 GRBs; (4) 17 of these 18 GRBs have redshifts of z>0.5, which are too far to be in the Local Supercluster. All these results disproved the hypothesis that any significant fraction of long-lag GRBs are from Local Supercluster. Hence these long-lag GRBs can not be counted in the calculation of LIGO detection rates. An explanation of why we can detect long-lag GRBs at high redshift is presented.Comment: 14 pages, 1 figure, ApJ accepte

Observation of contemporaneous optical radiation from a gamma-ray burst

The origin of gamma-ray bursts (GRBs) has been enigmatic since their discovery. The situation improved dramatically in 1997, when the rapid availability of precise coordinates for the bursts allowed the detection of faint optical and radio afterglows - optical spectra thus obtained have demonstrated conclusively that the bursts occur at cosmological distances. But, despite efforts by several groups, optical detection has not hitherto been achieved during the brief duration of a burst. Here we report the detection of bright optical emission from GRB990123 while the burst was still in progress. Our observations begin 22 seconds after the onset of the burst and show an increase in brightness by a factor of 14 during the first 25 seconds; the brightness then declines by a factor of 100, at which point (700 seconds after the burst onset) it falls below our detection threshold. The redshift of this burst, approximately 1.6, implies a peak optical luminosity of 5 times 10^{49} erg per second. Optical emission from gamma-ray bursts has been generally thought to take place at the shock fronts generated by interaction of the primary energy source with the surrounding medium, where the gamma-rays might also be produced. The lack of a significant change in the gamma-ray light curve when the optical emission develops suggests that the gamma-rays are not produced at the shock front, but closer to the site of the original explosion.Comment: 10 pages, 2 figures. Accepted for publication in Nature. For additional information see http://www.umich.edu/~rotse

A statistical study of gamma-ray burst afterglows measured by the Swift Ultra-violet Optical Telescope

We present the first statistical analysis of 27 UVOT optical/ultra-violet lightcurves of GRB afterglows. We have found, through analysis of the lightcurves in the observer's frame, that a significant fraction rise in the first 500s after the GRB trigger, that all lightcurves decay after 500s, typically as a power-law with a relatively narrow distribution of decay indices, and that the brightest optical afterglows tend to decay the quickest. We find that the rise could either be produced physically by the start of the forward shock, when the jet begins to plough into the external medium, or geometrically where an off-axis observer sees a rising lightcurve as an increasing amount of emission enters the observers line of sight, which occurs as the jet slows. We find that at 99.8% confidence, there is a correlation, in the observed frame, between the apparent magnitude of the lightcurves at 400s and the rate of decay after 500s. However, in the rest frame a Spearman Rank test shows only a weak correlation of low statistical significance between luminosity and decay rate. A correlation should be expected if the afterglows were produced by off-axis jets, suggesting that the jet is viewed from within the half-opening angle theta or within a core of uniform energy density theta_c. We also produced logarithmic luminosity distributions for three rest frame epochs. We find no evidence for bimodality in any of the distributions. Finally, we compare our sample of UVOT lightcurves with the XRT lightcurve canonical model. The range in decay indices seen in UVOT lightcurves at any epoch is most similar to the range in decay of the shallow decay segment of the XRT canonical model. However, in the XRT canonical model there is no indication of the rising behaviour observed in the UVOT lightcurves.Comment: 16 pages, 9 figures, accepted MNRA

The Host Galaxies of Short-Duration Gamma-Ray Bursts: Luminosities, Metallicities, and Star Formation Rates

The association of some short-duration gamma-ray bursts (GRBs) with elliptical galaxies established that their progenitors, unlike those of long GRBs, belong to an old stellar population. However, the majority of short GRBs appear to occur in star forming galaxies, raising the possibility that some progenitors are related to recent star formation activity. Here we present optical spectroscopy of these hosts and measure their luminosities, star formation rates, and metallicities. We find luminosities of L_B~0.1-1.5 L*, star formation rates of SFR~0.2-6 M_sun/yr, and metallicities of 12+log(O/H)~8.5-8.9 (Z~0.6-1.6 Z_sun). A detailed comparison to the hosts of long GRBs reveals systematically higher luminosities, lower specific star formation rates (SFR/L_B) by about an order of magnitude, and higher metallicities by about 0.6 dex. The K-S probability that the short and long GRB hosts are drawn from the same underlying galaxy distribution is only 10^-3. On the other hand, short GRB hosts exhibit excellent agreement with the specific star formation rates and the luminosity-metallicity relation of field galaxies at z~0.1-1. We thus conclude that short GRB hosts are not dominated by young stellar populations like long GRBs hosts. Instead, short GRB hosts appear to be drawn uniformly from the underlying galaxy distribution, indicating that the progenitors have a wide age distribution of several Gyr.Comment: Submitted to Ap

Framework for Leadership and Training of Biosafety Level 4 Laboratory Workers

One-sentence summary for table of contents: Training should include theoretical consideration of biocontainment principles, practical hands-on training, and mentored on-the-job experience

Demonstration of Universal Parametric Entangling Gates on a Multi-Qubit Lattice

We show that parametric coupling techniques can be used to generate selective entangling interactions for multi-qubit processors. By inducing coherent population exchange between adjacent qubits under frequency modulation, we implement a universal gateset for a linear array of four superconducting qubits. An average process fidelity of $\mathcal{F}=93\%$ is estimated for three two-qubit gates via quantum process tomography. We establish the suitability of these techniques for computation by preparing a four-qubit maximally entangled state and comparing the estimated state fidelity against the expected performance of the individual entangling gates. In addition, we prepare an eight-qubit register in all possible bitstring permutations and monitor the fidelity of a two-qubit gate across one pair of these qubits. Across all such permutations, an average fidelity of $\mathcal{F}=91.6\pm2.6\%$ is observed. These results thus offer a path to a scalable architecture with high selectivity and low crosstalk

Dust and Metal Column Densities in Gamma-Ray Burst Host Galaxies

In this paper we present the results from the analysis of a sample of 28 gamma-ray burst (GRB) afterglow spectral energy distributions, spanning the X-ray through to near-infrared wavelengths. This is the largest sample of GRB afterglow spectral energy distributions thus far studied, providing a strong handle on the optical depth distribution of soft X-ray absorption and dust-extinction systems in GRB host galaxies. We detect an absorption system within the GRB host galaxy in 79% of the sample, and an extinction system in 71% of the sample, and find the Small Magellanic Cloud (SMC) extinction law to provide an acceptable fit to the host galaxy extinction profile for the majority of cases, consistent with previous findings. The range in the soft X-ray absorption to dust-extinction ratio, N_{H,X}/Av, in GRB host galaxies spans almost two orders of magnitude, and the typical ratios are significantly larger than those of the Magellanic Clouds or Milky Way. Although dust destruction could be a cause, at least in part, for the large N_{H,X}/Av ratios, the good fit provided by the SMC extinction law for the majority of our sample suggests that there is an abundance of small dust grains in the GRB environment, which we would expect to have been destroyed if dust destruction were responsible for the large N_{H,X}/Av ratios. Instead, our analysis suggests that the distribution of N_{H,X}/Av in GRB host galaxies may be mostly intrinsic to these galaxies, and this is further substantiated by evidence for a strong negative correlation between N_{H,X}/Av and metallicity for a subsample of GRB hosts with known metallicity. Furthermore, we find the N_{H,X}/Av ratio and metallicity for this subsample of GRBs to be comparable to the relation found in other more metal-rich galaxies.Comment: 23 pages, 10 figures, accepted for publication in MNRA

Assessment of higher order cognitive skills in undergraduate education: modified essay or multiple choice questions? Research paper

Background: Reliable and valid written tests of higher cognitive function are difficult to produce, particularly for the assessment of clinical problem solving. Modified Essay Questions (MEQs) are often used to assess these higher order abilities in preference to other forms of assessment, including multiple-choice questions (MCQs). MEQs often form a vital component of end-of-course assessments in higher education. It is not clear how effectively these questions assess higher order cognitive skills. This study was designed to assess the effectiveness of the MEQ to measure higher-order cognitive skills in an undergraduate institution. Methods: An analysis of multiple-choice questions and modified essay questions (MEQs) used for summative assessment in a clinical undergraduate curriculum was undertaken. A total of 50 MCQs and 139 stages of MEQs were examined, which came from three exams run over two years. The effectiveness of the questions was determined by two assessors and was defined by the questions ability to measure higher cognitive skills, as determined by a modification of Bloom's taxonomy, and its quality as determined by the presence of item writing flaws. Results: Over 50% of all of the MEQs tested factual recall. This was similar to the percentage of MCQs testing factual recall. The modified essay question failed in its role of consistently assessing higher cognitive skills whereas the MCQ frequently tested more than mere recall of knowledge. Conclusion: Construction of MEQs, which will assess higher order cognitive skills cannot be assumed to be a simple task. Well-constructed MCQs should be considered a satisfactory replacement for MEQs if the MEQs cannot be designed to adequately test higher order skills. Such MCQs are capable of withstanding the intellectual and statistical scrutiny imposed by a high stakes exit examination.Edward J Palmer, Peter G Devit