Measuring the Effect of ITPP on Tumor Hypoxia with Multispectral Optoacoustic Tomography

https://openworks.mdanderson.org/sumexp22/1035/thumbnail.jp

Detecting regular sound changes in linguistics as events of concerted evolution

Background: Concerted evolution is normally used to describe parallel changes at different sites in a genome, but it is also observed in languages where a specific phoneme changes to the same other phoneme in many words in the lexicon—a phenomenon known as regular sound change. We develop a general statistical model that can detect concerted changes in aligned sequence data and apply it to study regular sound changes in the Turkic language family. Results: Linguistic evolution, unlike the genetic substitutional process, is dominated by events of concerted evolutionary change. Our model identified more than 70 historical events of regular sound change that occurred throughout the evolution of the Turkic language family, while simultaneously inferring a dated phylogenetic tree. Including regular sound changes yielded an approximately 4-fold improvement in the characterization of linguistic change over a simpler model of sporadic change, improved phylogenetic inference, and returned more reliable and plausible dates for events on the phylogenies. The historical timings of the concerted changes closely follow a Poisson process model, and the sound transition networks derived from our model mirror linguistic expectations. Conclusions: We demonstrate that a model with no prior knowledge of complex concerted or regular changes can nevertheless infer the historical timings and genealogical placements of events of concerted change from the signals left in contemporary data. Our model can be applied wherever discrete elements—such as genes, words, cultural trends, technologies, or morphological traits—can change in parallel within an organism or other evolving group

Development of a “Universal” Phantom for Standardization of Chemical Exchange Saturation Transfer (CEST) MRI

https://openworks.mdanderson.org/sumexp22/1039/thumbnail.jp

The Origin of the Mass--Metallicity Relation: Insights from 53,000 Star-Forming Galaxies in the SDSS

We utilize Sloan Digital Sky Survey imaging and spectroscopy of ~53,000 star-forming galaxies at z~0.1 to study the relation between stellar mass and gas-phase metallicity. We derive gas-phase oxygen abundances and stellar masses using new techniques which make use of the latest stellar evolutionary synthesis and photoionization models. We find a tight (+/-0.1 dex) correlation between stellar mass and metallicity spanning over 3 orders of magnitude in stellar mass and a factor of 10 in metallicity. The relation is relatively steep from 10^{8.5} - 10^{10.5} M_sun, in good accord with known trends between luminosity and metallicity, but flattens above 10^{10.5} M_sun. We use indirect estimates of the gas mass based on the H-alpha luminosity to compare our data to predictions from simple closed box chemical evolution models. We show that metal loss is strongly anti-correlated with baryonic mass, with low mass dwarf galaxies being 5 times more metal-depleted than L* galaxies at z~0.1. Evidence for metal depletion is not confined to dwarf galaxies, but is found in galaxies with masses as high as 10^{10} M_sun. We interpret this as strong evidence both of the ubiquity of galactic winds and of their effectiveness in removing metals from galaxy potential wells.Comment: ApJ accepted, 15 pages, 9 figures, emulateapj.st

The Recent Star Formation in NGC 6822: an Ultraviolet Study

We characterize the star formation in the low-metallicity galaxy NGC 6822 over the past few hundred million years, using GALEX far-UV (FUV, 1344-1786 A) and near-UV (NUV, 1771-2831 A) imaging, and ground-based Ha imaging. From GALEX FUV image, we define 77 star-forming (SF) regions with area >860 pc^2, and surface brightness <=26.8 mag(AB)arcsec^-2, within 0.2deg (1.7kpc) of the center of the galaxy. We estimate the extinction by interstellar dust in each SF region from resolved photometry of the hot stars it contains: E(B-V) ranges from the minimum foreground value of 0.22mag up to 0.66+-0.21mag. The integrated FUV and NUV photometry, compared with stellar population models, yields ages of the SF complexes up to a few hundred Myr, and masses from 2x10^2 Msun to 1.5x10^6 Msun. The derived ages and masses strongly depend on the assumed type of interstellar selective extinction, which we find to vary across the galaxy. The total mass of the FUV-defined SF regions translates into an average star formation rate (SFR) of 1.4x10^-2 Msun/yr over the past 100 Myr, and SFR=1.0x10^-2 Msun/yr in the most recent 10 Myr. The latter is in agreement with the value that we derive from the Ha luminosity, SFR=0.008 Msun/yr. The SFR in the most recent epoch becomes higher if we add the SFR=0.02 Msun/yr inferred from far-IR measurements, which trace star formation still embedded in dust (age <= a few Myr).Comment: Accepted for publication in ApJ, 21 pages, 6 figures, 3 table

Early Detection of Pancreatic Intraepithelial Neoplasias (PanINs) in Transgenic Mouse Model by Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy

While pancreatic cancer (PC) survival rates have recently shown modest improvement, the disease remains largely incurable. Early detection of pancreatic cancer may result in improved outcomes and therefore, methods for early detection of cancer, even premalignant lesions, may provide more favorable outcomes. Pancreatic intraepithelial neoplasias (PanINs) have been identified as premalignant precursor lesions to pancreatic cancer. However, conventional imaging methods used for screening high-risk populations do not have the sensitivity to detect PanINs. Here, we have employed hyperpolarized metabolic imaging in vivo and nuclear magnetic resonance (1H-NMR) metabolomics ex vivo to identify and understand metabolic changes, towards enabling detection of early PanINs and progression to advanced PanINs lesions that precede pancreatic cancer formation. Progression of disease from tissue containing predominantly low-grade PanINs to tissue with high-grade PanINs showed a decreasing alanine/lactate ratio from high-resolution NMR metabolomics ex vivo. Hyperpolarized magnetic resonance spectroscopy (HP-MRS) allows over 10,000-fold sensitivity enhancement relative to conventional magnetic resonance. Real-time HP-MRS was employed to measure non-invasively changes of alanine and lactate metabolites with disease progression and in control mice in vivo, following injection of hyperpolarized [1-13C] pyruvate. The alanine-to-lactate signal intensity ratio was found to decrease as the disease progressed from low-grade PanINs to high-grade PanINs. The biochemical changes of alanine transaminase (ALT) and lactate dehydrogenase (LDH) enzyme activity were assessed. These results demonstrate that there are significant alterations of ALT and LDH activities during the transformation from early to advanced PanINs lesions. Furthermore, we demonstrate that real-time conversion kinetic rate constants (kPA and kPL) can be used as metabolic imaging biomarkers of pancreatic premalignant lesions. Findings from this emerging HP-MRS technique can be translated to the clinic for detection of pancreatic premalignant lesion in high-risk populations.Fil: Dutta, Prasanta. University of Texas; Estados UnidosFil: Castro Pando, Susana. University of Texas; Estados UnidosFil: Mascaró, Marilina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. University of Texas; Estados UnidosFil: Riquelme, Erick. University of Texas; Estados UnidosFil: Zoltan, Michelle. University of Texas; Estados UnidosFil: Zacharias, Niki M.. University of Texas; Estados UnidosFil: Gammon, Seth T.. University of Texas; Estados UnidosFil: Piwnica-Worms, David. University of Texas; Estados UnidosFil: Pagel, Mark D.. University of Texas; Estados UnidosFil: Sen, Subrata. University of Texas; Estados UnidosFil: Maitra, Anirban. University of Texas; Estados UnidosFil: Shams, Shayan. University of Texas; Estados UnidosFil: McAllister, Florencia. University of Texas; Estados UnidosFil: Bhattacharya, Pratip K.. University of Texas; Estados Unido

Chemical and Photometric Evolution of Extended Ultraviolet Disks: Optical Spectroscopy of M83 (NGC5236) and NGC4625

We present the results from the analysis of optical spectra of 31 Halpha-selected regions in the extended UV (XUV) disks of M83 (NGC5236) and NGC4625 recently discovered by GALEX. The spectra were obtained using IMACS at Las Campanas Observatory 6.5m Magellan I telescope and COSMIC at the Palomar 200-inch telescope, respectively for M83 and NGC4625. The line ratios measured indicate nebular oxygen abundances (derived from the R23 parameter) of the order of Zsun/5-Zsun/10. For most emission-line regions analyzed the line fluxes and ratios measured are best reproduced by models of photoionization by single stars with masses in the range 20-40 Msun and oxygen abundances comparable to those derived from the R23 parameter. We find indications for a relatively high N/O abundance ratio in the XUV disk of M83. Although the metallicities derived imply that these are not the first stars formed in the XUV disks, such a level of enrichment could be reached in young spiral disks only 1 Gyr after these first stars would have formed. The amount of gas in the XUV disks allow maintaining the current level of star formation for at least a few Gyr.Comment: 52 pages, 8 tables, 7 figures, accepted for publication in Ap

The Society for Immunotherapy of Cancer (SITC) clinical practice guideline on immunotherapy for the treatment of acute leukemia.

Acute leukemia is a constellation of rapidly progressing diseases that affect a wide range of patients regardless of age or gender. Traditional treatment options for patients with acute leukemia include chemotherapy and hematopoietic cell transplantation. The advent of cancer immunotherapy has had a significant impact on acute leukemia treatment. Novel immunotherapeutic agents including antibody-drug conjugates, bispecific T cell engagers, and chimeric antigen receptor T cell therapies have efficacy and have recently been approved by the US Food and Drug Administration (FDA) for the treatment of patients with acute leukemia. The Society for Immunotherapy of Cancer (SITC) convened a panel of experts to develop a clinical practice guideline composed of consensus recommendations on immunotherapy for the treatment of acute lymphoblastic leukemia and acute myeloid leukemia

The Rest Frame Optical Spectra of Lyman Break Galaxies: Star Formation, Extinction, Abundances, and Kinematics

We present the first results of a spectroscopic survey of Lyman break galaxies in the near-infrared aimed at detecting the emission lines of [O II], [O III], and Hbeta from the H II regions of star forming galaxies at z = 3. From observations of 19 objects with the Keck and VLT telescopes, we reach the following main conclusions. Contrary to expectations, the star formation rates deduced from the Hbeta luminosity are on average no larger than those implied by the stellar continuum at 1500 A; presumably any differential extinction between rest-frame optical and UV is small compared with the relative uncertainties in the calibrations of these two star formation tracers. For the galaxies in our sample, the abundance of O can only be determined to within one order of magnitude. Even so, it seems well established that LBGs are the most metal-enriched structures at z = 3, apart from QSOs, with abundances greater than about 1/10 solar. They are also significantly overluminous for their metallicities; this is probably an indication that their mass-to-light ratios are small compared with present-day galaxies. At face value their velocity dispersions, sigma = 50 - 115 km/s imply virial masses of about 10^{10} solar masses within half-light radii of 2.5 kpc. However, we are unable to establish if the widths of the emission lines do reflect the motions of the H II regions within the gravitational potential of the galaxies, even though in two cases we see hints of rotation curves. All 19 LBGs observed show evidence for galactic-scale superwinds; such outflows are important for regulating star formation, distributing metals over large volumes, and allowing Lyman continuum photons to escape and ionize the IGM.Comment: 44 pages, LaTeX, 13 Postscript Figures. Accepted for publication in the Astrophysical Journal, Vol. 554, No. 2 (Jun 20, 2001

Anti-CD45 Pretargeted Radioimmunotherapy Prior to Bone Marrow Transplantation without Total Body Irradiation Facilitates Engraftment From Haploidentical Donors and Prolongs Survival in a Disseminated Murine Leukemia Model

s / Biol Blood Marrow Transplant 19 (2013) S211eS232 S228 chemotherapy was HIDAC (1-3 grams/m2 for 6-8 doses)/ Etoposide(15-40mg/kg) in 16 patients and growth factor alone in one patient. Median time from diagnosis to ASCT was 4.2 (range 3.6-7) months. Preparative regimen for ASCT was Busulfan (3.2mg/kg x 4)/Etoposide (60 mg/kg) in 12 patients and high dose melphalan in 5 patients. The median CD34 cells infused was 4.9 x 10e6/kg (range 2.8 to 15.9).All patients engrafted with a median time to neutrophil engraftment of 11 (range10-12) days. The median time to platelet engraftment was 20 (range15-40) days. The median length of inpatient stay during the ASCT admission was 14 (range 10-25) days. One patient died of progressive disease 14 months post ASCT. Two patients died in remission on day 53 (sepsis) and day 836 (unknown cause) post ASCT. Fourteen patients (82%) are currently alive in complete remission. at a median follow-up of 20 (range 140) months post ASCT. Conclusion: Consolidation of good risk AML patients with ASCT following induction of complete remission is safe and effective in preventing relapse in good risk AML patients