A proactive approach to the conservation of historic and cultural Heritage: The HeritageCare methodology

Presently, no systematic policy for the preventive conservation of the built cultural heritage exists in South-West Europe. Current approaches for inspection, diagnosis, monitoring and reactive conservation are normally intermittent, unplanned, expensive and lack methodical strategy. The available financial resources are scarce and are mostly addressed to listed buildings. Besides, owners and stakeholders often reveal reluctance to invest in preventive conservation and maintenance programs. In view of these considerations, and driven by the principle “prevention is better than cure”, the HeritageCare project has developed a system for the preventive conservation and maintenance of the built heritage. The main aim of this paper is to briefly describe the methodology, including its three levels of service, and present the main results of the implementation and validation of the service level 1 on a case study belonging to the Portuguese architectural heritage.This work was carried out within the scope of the project HeritageCare (SOE1/P5/P0258), co-funded by the Interreg-Sudoe/FEDER program and included in the research activities of the ISISE Research Centre, also financed by FEDER funds through the Competitiveness Factors Operational Program – COMPETE and by national funds through FCT – Foundation for Science and Technology within the scope of the project POCI-01-0145-FEDER-007633. Authors would like to express their gratitude to the Centre for Computer Graphics, HeritageCare partner, for the development of the platform (and supporting database) and the mobile application, outputs of the project HeritageCare. Authors would like to express their gratitude to the Santa Maria de la Real Foundation, HeritageCare partner, for the placement of the monitoring system at the Ducal Palace, Guimarães, Portugal

Ensaios de proveniência de sobreiro (Quercus suber) - resultados aos cinco anos

Congresso Florestal Nacional: a floresta e as gentes - Actas das ComunicaçõesA adaptabilidade do sobreiro às condições ambientais está pouco estudada e o uso de recursos genéticos inapropriados é uma das causas do insucesso de reflorestação tanto no curto como no longo prazo. Os resultados do esforço de florestação com sobreiro têm sido muito díspares, com valores médios de sobrevivência de 50% que oscilam entre o insucesso total e uma sobrevivência que, em alguns casos, atinge os 100%. A eventual alteração climática acentua a necessidade da utilização de material de reprodução adaptado, como meio de promover a sustentabilidade do montado e das florestas de sobreiro. A caracterização dos recursos genéticos desta espécie poderá contribuir também para a viabilidade económica e ambiental deste sistema ao identificar as populações mais adaptadas e os indivíduos produtores de melhor cortiça. A rede de ensaios de proveniência de sobreiro instalados em 1998, no âmbito da acção concertada “FAIR 1CT 95 0202”, onde estão representadas 35 populações cobrindo toda a sua área de distribuição natural são um excelente instrumento para a avaliação da adaptabilidade. Ainda que as respostas ao nível da qualidade da cortiça só possam ser obtidas tardiamente, o acompanhamento de parâmetros adaptativos ao longo do crescimento das árvores é importante e poderá fornecer informação relevante para a compreensão do funcionamento do sistema. Nesta comunicação apresentam-se os resultados obtidos, 5 anos após a instalação, nos ensaios de proveniência estabelecidos na Mata Nacional das Virtudes e no Monte da Fava relativamente a características adaptativas tais como: a sobrevivência, a eficiência do uso da água, o abrolhamento dos gomos foliares e o crescimento. Ao nível da sobrevivência as populações de origem francesa revelaram-se as menos adaptadas em ambos os locais. Quanto à altura, as 35 populações apresentaram crescimentos significativamente diferentes entre si nos dois ensaios, tendo as populações marroquinas registado os valores mais elevados. Relativamente ao abrolhamento dos gomos foliares observou-se um comportamento semelhante em termos das populações mais tardias e mais temporãs em ambos os ensaios. Geograficamente as primeiras localizam-se numa área mais ocidental da zona de distribuição natural da espécie enquanto que populações mais temporãs, com comportamento significativamente diferente das anteriores são originárias da região mais oriental

Luminescence studies on green emitting InGaN/GaN MQWs implanted with nitrogen

We studied the optical properties of metalorganic chemical vapour deposited (MOCVD) InGaN/GaN multiple quantum wells (MQW) subjected to nitrogen (N) implantation and post-growth annealing treatments. The optical characterization was carried out by means of temperature and excitation density-dependent steady state photoluminescence (PL) spectroscopy, supplemented by room temperatura PL excitation (PLE) and PL lifetime (PLL) measurements. The as-grown and as-implanted samples were found to exhibit a single green emission band attributed to localized excitons in the QW, although the N implantation leads to a strong reduction of the PL intensity. The green band was found to be surprisingly stable on annealing up to 14006C. A broad blue band dominates the low temperature PL after termal annealing in both samples. This band is more intense for the implanted sample, suggesting that defects generated by N implantation, likely related to the diffusion/segregation of indium (In), have been optically activated by the thermal treatmentThe authors acknowledge FCT for the final funding from PEst-C/CTM/LA0025/2013-14, PTDC/CTM-NAN/2156/2012, PTDC/FIS-NAN/0973/2012 and RECI/FIS-NAN/0183/ 2012 (FCOMP-01-0124-FEDER-027494) projects. J. Rodrigues thanks FCT for her PhD grant, SFRH/BD/76300/2011. ARC acknowledges financial support under the ‘Juan de la Cierva’ program (MECO, Spain) through grant JCI-2012-14509

West Nile virus transmission potential in Portugal

© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.It is unclear whether West Nile virus (WNV) circulates endemically in Portugal. Despite the country's adequate climate for transmission, Portugal has only reported four human WNV infections so far. We performed a review of WNV-related data (1966-2020), explored mosquito (2016-2019) and land type distributions (1992-2019), and used climate data (1981-2019) to estimate WNV transmission suitability in Portugal. Serological and molecular evidence of WNV circulation from animals and vectors was largely restricted to the south. Land type and climate-driven transmission suitability distributions, but not the distribution of WNV-capable vectors, were compatible with the North-South divide present in serological and molecular evidence of WNV circulation. Our study offers a comprehensive, data-informed perspective and review on the past epidemiology, surveillance and climate-driven transmission suitability of WNV in Portugal, highlighting the south as a subregion of importance. Given the recent WNV outbreaks across Europe, our results support a timely change towards local, active surveillance.info:eu-repo/semantics/publishedVersio

Predictors of cardiac involvement in idiopathic inflammatory myopathies

Copyright © 2023 Bandeira, Dourado, Melo, Martins, Fraga, Ferraro, Saraiva, Sousa, Parente, Soares, Correia, Almeida, Dinis, Pinto, Oliveira Pinheiro, Rato, Beirão, Samões, Santos, Mazeda, Chícharo, Faria, Neto, Lourenço, Brites, Rodrigues, Silva-Dinis, Dias, Araújo, Martins, Couto, Valido, Santos, Barreira, Fonseca and Campanilho-Marques. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Objectives: Idiopathic inflammatory myopathies (IIM) are a group of rare disorders that can affect the heart. This work aimed to find predictors of cardiac involvement in IIM. Methods: Multicenter, open cohort study, including patients registered in the IIM module of the Rheumatic Diseases Portuguese Register (Reuma.pt/Myositis) until January 2022. Patients without cardiac involvement information were excluded. Myo(peri)carditis, dilated cardiomyopathy, conduction abnormalities, and/or premature coronary artery disease were considered. Results: 230 patients were included, 163 (70.9%) of whom were females. Thirteen patients (5.7%) had cardiac involvement. Compared with IIM patients without cardiac involvement, these patients had a lower bilateral manual muscle testing score (MMT) at the peak of muscle weakness [108.0 ± 55.0 vs 147.5 ± 22.0, p=0.008] and more frequently had oesophageal [6/12 (50.0%) vs 33/207 (15.9%), p=0.009] and lung [10/13 (76.9%) vs 68/216 (31.5%), p=0.001] involvements. Anti-SRP antibodies were more commonly identified in patients with cardiac involvement [3/11 (27.3%) vs 9/174 (5.2%), p=0.026]. In the multivariate analysis, positivity for anti-SRP antibodies (OR 104.3, 95% CI: 2.5-4277.8, p=0.014) was a predictor of cardiac involvement, regardless of sex, ethnicity, age at diagnosis, and lung involvement. Sensitivity analysis confirmed these results. Conclusion: Anti-SRP antibodies were predictors of cardiac involvement in our cohort of IIM patients, irrespective of demographical characteristics and lung involvement. We suggest considering frequent screening for heart involvement in anti-SRP-positive IIM patients.info:eu-repo/semantics/publishedVersio

Long-term declines of wader populations at the Tagus estuary, Portugal: A response to global or local factors?

Migratory wader populations face global threats, mainly related to increasing rates of habitat loss and disturbance driven by human activities. To a large extent, the long-term survival of these populations requires the conservation of networks of sites along their migratory flyways. The Tagus estuary, Portugal, is among the most important wetlands for waders in the East Atlantic Flyway. Annual winter wader counts have been carried in this wetland since 1975 and a monthly roost-monitoring programme was implemented in 2007. Wintering populations of three out of the five most abundant species, Dunlin Calidris alpina, Grey Plover Pluvialis squatarola and Redshank Tringa totanus, showed significant population declines over the past three decades, which are most likely due to the loss and degradation of roost sites as a result of increasing human activity. The situation is unlikely to improve, as a high proportion of the wintering waders use roost sites that are situated in highly urbanised areas with no legal protection. The use of different roost sites by waders is highly variable both temporally and spatially, thus emphasizing the need for a network of good quality roost sites. Additionally, during migration, 60–80% of all the waders of the Tagus estuary concentrate at a single refuge, thus increasing the risk for wader populations during these periods. ------ RESUMO ------ As populações de aves limícolas migradoras enfrentam ameaças globais, maioritariamente relacionadas com a crescente perda de habitat e perturbação humana. Para garantir a sobrevivência a longo-termo destas populações é imprescindível a conservação de uma rede de áreas húmidas ao longo das suas rotas migratórias. O estuário do Tejo, em Portugal, é uma das mais importantes áreas húmidas para aves limícolas na rota Migratória do Atlântico Este. Desde 1975, foram efectuadas contagens anuais de aves limícolas no inverno nesta zona húmida e, em 2007, foi implementado um programa de monitorização mensal dos refúgios de preia-mar. As populações invernantes de três das cinco espécies de limícolas mais abundantes no estuário, o Pilrito-de-peito-preto Calidris alpina, a Tarambola-cinzenta Pluvialis squatarola e o Perna-vermelha Tringa totanus, mostraram tendências significativas de decréscimo nas últimas três décadas, potencialmente devido à degradação e perda de refúgios como resultado da crescente actividade antropogénica. Esta situação poderá deteriorar-se, uma vez que uma grande proporção das aves limícolas invernantes utiliza refúgios em áreas densamente urbanizadas sem qualquer estatuto legal de protecção. A utilização dos diferentes refúgios do estuário do Tejo por aves limícolas tem uma grande variação espacial e temporal, o que reforça a importância da existência de uma rede de refúgios de elevada qualidade nesta área húmida. Durante a migração, 60 a 80% das aves limícolas concentram-se num único refúgio, ficando assim particularmente vulneráveis neste período

Valorização do patrimônio científico e tecnológico brasileiro: descobrindo conjuntos de objetos de C&T pelo Brasil

Most of Brazil's science and technology (S&T) heritage has yet to be tracked down. The knowledge amassed on the topic is still limited, and there is a real risk that Brazilian science and technology objects may already have been modernized or discarded. A research project was developed in the last four years including several initiatives to preserve this kind of heritage, as such: a national survey to build up a picture of the current state of this heritage; studies concerning heritage protection legislation of some countries, including Brazil; and two case studies of collections of S&T objects (Observatório do Valongo and Colégio Pedro II). This paper presents an overview of the results obtained in the several initiatives of preservation undertaken. The survey is divided into different areas of knowledge and different time frames. As such, we are interested in sets of objects from the exact sciences, the different engineering specialties, as well as geography, geology and oceanography, which were manufactured in or before the 1960s. A registration form was prepared which was adapted from a form used in surveys of collections of this kind in Portugal. Sets of objects have been identified at museums, universities and research institutes. From the results, it could be observed that: the best preserved collections are at the few museums devoted to the area, but the universities hold most of the artifacts and the overwhelming majority of the objects were made in the twentieth century.A maior parte dos bens que constituem o patrimônio cultural da Ciência e Tecnologia (C&T) está para ser descoberta. O conhecimento acumulado sobre o tema ainda é limitado e há um risco real de que os objetos já tenham sido modernizados ou descartados. Nos últimos quatro anos, desenvolveu-se um projeto de pesquisa, envolvendo várias iniciativas para preservar este tipo de patrimônio: um levantamento nacional para construir um panorama do estado atual desse patrimônio; estudos sobre a legislação de proteção ao patrimônio de alguns países, inclusive o Brasil; e dois estudos de caso de coleções de objetos de C&T (Observatório do Valongo e Colégio Pedro II, ambos no Rio de Janeiro). Este artigo apresenta uma visão geral dos resultados obtidos e, em especial, dos levantamentos de conjuntos de objetos de C&T. Utilizou-se um recorte em relação a áreas do conhecimento e período histórico, compreendendo objetos pertencentes à s ciências exatas, à s diferentes especialidades de engenharia, bem como geografia, geologia e oceanografia, e fabricados até 1960. A partir da adaptação de um formulário usado em pesquisas similares em Portugal, elaborou-se uma ficha de registro, que permitiu identificar conjuntos de objetos em museus, universidades e institutos de pesquisa. Pôde-se, com isso, observar que as coleções mais bem preservadas estão nos poucos museus dedicados à área, enquanto as universidades detêm a maior parte dos artefatos, e que a esmagadora maioria dos objetos foi produzida no século XX

Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns

Abstract Background The molecular phylogenetic relationships and population structure of the species of the Anopheles triannulatus complex: Anopheles triannulatus s.s., Anopheles halophylus and the putative species Anopheles triannulatus C were investigated. Methods\ud \ud The mitochondrial COI gene, the nuclear white gene and rDNA ITS2 of samples that include the known geographic distribution of these taxa were analyzed. Phylogenetic analyses were performed using Bayesian inference, Maximum parsimony and Maximum likelihood approaches. Results Each data set analyzed septely yielded a different topology but none provided evidence for the seption of An. halophylus and An. triannulatus C, consistent with the hypothesis that the two are undergoing incipient speciation. The phylogenetic analyses of the white gene found three main clades, whereas the statistical parsimony network detected only a single metapopulation of Anopheles triannulatus s.l. Seven COI lineages were detected by phylogenetic and network analysis. In contrast, the network, but not the phylogenetic analyses, strongly supported three ITS2 groups. Combined data analyses provided the best resolution of the trees, with two major clades, Amazonian (clade I) and trans-Andean + Amazon Delta (clade II). Clade I consists of multiple subclades: An. halophylus + An. triannulatus C; trans-Andean Venezuela; central Amazonia + central Bolivia; Atlantic coastal lowland; and Amazon delta. Clade II includes three subclades: Panama; cis-Andean Colombia; and cis-Venezuela. The Amazon delta specimens are in both clades, likely indicating local sympatry. Spatial and molecular variance analyses detected nine groups, corroborating some of subclades obtained in the combined data analysis. Conclusion Combination of the three molecular markers provided the best resolution for differentiation within An. triannulatus s.s. and An. halophylus and C. The latest two species seem to be very closely related and the analyses performed were not conclusive regarding species differentiation. Further studies including new molecular markers would be desirable to solve this species status question. Besides, results of the study indicate a trans-Andean origin for An. triannulatus s.l. The potential implications for malaria epidemiology remain to be investigated.This project has been financially supported by NIH grant AI ROI 54139–02 to JE Conn, PAPES IV Fiocruz-CNPq to RLO, and from the Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP (Grant 2011/20397-7 to MAMS), CNPq (BPP no. 301666/2011-3 to MAMS).This research was performed under a Memorandum of Understanding between the Walter Reed Army Institute of Research and the Smithsonian Institution, with institutional support provided by both organizations. The material to be published reflects the views of the authors and should not be construed to represent those of the Department of the Army or the Department of Defense

Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns

Abstract Background The molecular phylogenetic relationships and population structure of the species of the Anopheles triannulatus complex: Anopheles triannulatus s.s., Anopheles halophylus and the putative species Anopheles triannulatus C were investigated. Methods\ud \ud The mitochondrial COI gene, the nuclear white gene and rDNA ITS2 of samples that include the known geographic distribution of these taxa were analyzed. Phylogenetic analyses were performed using Bayesian inference, Maximum parsimony and Maximum likelihood approaches. Results Each data set analyzed septely yielded a different topology but none provided evidence for the seption of An. halophylus and An. triannulatus C, consistent with the hypothesis that the two are undergoing incipient speciation. The phylogenetic analyses of the white gene found three main clades, whereas the statistical parsimony network detected only a single metapopulation of Anopheles triannulatus s.l. Seven COI lineages were detected by phylogenetic and network analysis. In contrast, the network, but not the phylogenetic analyses, strongly supported three ITS2 groups. Combined data analyses provided the best resolution of the trees, with two major clades, Amazonian (clade I) and trans-Andean + Amazon Delta (clade II). Clade I consists of multiple subclades: An. halophylus + An. triannulatus C; trans-Andean Venezuela; central Amazonia + central Bolivia; Atlantic coastal lowland; and Amazon delta. Clade II includes three subclades: Panama; cis-Andean Colombia; and cis-Venezuela. The Amazon delta specimens are in both clades, likely indicating local sympatry. Spatial and molecular variance analyses detected nine groups, corroborating some of subclades obtained in the combined data analysis. Conclusion Combination of the three molecular markers provided the best resolution for differentiation within An. triannulatus s.s. and An. halophylus and C. The latest two species seem to be very closely related and the analyses performed were not conclusive regarding species differentiation. Further studies including new molecular markers would be desirable to solve this species status question. Besides, results of the study indicate a trans-Andean origin for An. triannulatus s.l. The potential implications for malaria epidemiology remain to be investigated.This project has been financially supported by NIH grant AI ROI 54139–02 to JE Conn, PAPES IV Fiocruz-CNPq to RLO, and from the Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP (Grant 2011/20397-7 to MAMS), CNPq (BPP no. 301666/2011-3 to MAMS).This research was performed under a Memorandum of Understanding between the Walter Reed Army Institute of Research and the Smithsonian Institution, with institutional support provided by both organizations. The material to be published reflects the views of the authors and should not be construed to represent those of the Department of the Army or the Department of Defense