Microbiological, biochemical and compositional changes during ripening of São Jorge – a raw milk cheese from the Azores (Portugal)

The microbial, compositional and biochemical profiles of São Jorge cheese (PDO) obtained from three distinct cheese plants, throughout the ripening period were determined. Fully ripened cheeses (i.e. by 130 days) contained a total of 3.1 107 CFU g 1 mesophilic bacteria, and a decrease in moisture content, concomitantly with an increase in salt content, was observed throughout the same time frame. The pH decreased until 30 days of ripening; thereafter, a slight increase was reported, up to 5.6 by the end of ripening. Urea-PAGE results showed extensive primary proteolysis, of both b-casein and as1-casein degraded at essentially similar rates; plasmin and chymosin accordingly appear to be active in the cheese curd. RP-HPLC profiles of water-soluble fractions showed minor differences between 1 and 130 day old cheeses, whereas equivalent profiles of 7% (v/v) ethanol-soluble fractions contained several peaks, indicative of a heterogeneous mixture of products of proteolysis, that evolved with time

Utilização de plantas medicinais pela população rural no sudoeste paranaense

O conhecimento e a utilização de fitoterápicos como finalidade medicinal acompanha a humanidade desde os primórdios, com objetivos de prevenir e tratar doenças. Na maioria dos casos as plantas tiveram seus princípios ativos descobertos de maneira experimental e intuitiva, baseado em conhecimentos populares. O objetivo deste trabalho foi realizar um levantamento das 10 (dez) principais plantas medicinais utilizadas pelos moradores da comunidade de São Francisco do Bandeira no município de Dois Vizinhos, Paraná. As plantas mais utilizadas foram: Sálvia (Salvia officinalis), Erva-macaé (Leonuros Sibiricus), Quebra- pedra (Phyllanthus spp), Malva (Malva sylvestris), Guaco (Mikania glomerata), Paripiroba (Piper umbellatum), Pata de vaca (Bauhinia forficata), Bálsamo-branco (Sedum dendroideum), Babosa (Aloe arborescens) Poejo (Mentha pulegium), Amora folha (Morus alba). A pesquisa concluiu que ervas medicinais são muito utilizadas tanto de forma preventiva quanto curativa.Knowledge and use of herbal medicinal purpose as accompanies humanity since the beginning, with goals of preventing and treating diseases. In most cases the plants had their active principles discovered experimentally and intuitively, based on popular knowledge. This study aimed to survey the ten (10) main medicinal plants used by the community residents of San Francisco do Bandeira in the city of Dois Vizinhos, Paraná. The most commonly used plants were: Sage (Salvia officinalis), Herb-macaé (Leonuros sibiricus), breaking stone (Phyllanthus spp), Mallow (Malva sylvestris), Guaco (Mikania glomerata), Paripiroba (Piper umbellatum), cow foot (Bauhinia forficata), Balm white (Sedum dendroideum), Aloe (Aloe arborescens) Pennyroyal (Mentha pulegium), Mulberry leaf (Morus alba). The research concluded that medicinal herbs are widely used both preventively as curative.Eje A5: Sistemas de conocimientoFacultad de Ciencias Agrarias y Forestale

Utilização de plantas medicinais pela população rural no sudoeste paranaense

O conhecimento e a utilização de fitoterápicos como finalidade medicinal acompanha a humanidade desde os primórdios, com objetivos de prevenir e tratar doenças. Na maioria dos casos as plantas tiveram seus princípios ativos descobertos de maneira experimental e intuitiva, baseado em conhecimentos populares. O objetivo deste trabalho foi realizar um levantamento das 10 (dez) principais plantas medicinais utilizadas pelos moradores da comunidade de São Francisco do Bandeira no município de Dois Vizinhos, Paraná. As plantas mais utilizadas foram: Sálvia (Salvia officinalis), Erva-macaé (Leonuros Sibiricus), Quebra- pedra (Phyllanthus spp), Malva (Malva sylvestris), Guaco (Mikania glomerata), Paripiroba (Piper umbellatum), Pata de vaca (Bauhinia forficata), Bálsamo-branco (Sedum dendroideum), Babosa (Aloe arborescens) Poejo (Mentha pulegium), Amora folha (Morus alba). A pesquisa concluiu que ervas medicinais são muito utilizadas tanto de forma preventiva quanto curativa.Knowledge and use of herbal medicinal purpose as accompanies humanity since the beginning, with goals of preventing and treating diseases. In most cases the plants had their active principles discovered experimentally and intuitively, based on popular knowledge. This study aimed to survey the ten (10) main medicinal plants used by the community residents of San Francisco do Bandeira in the city of Dois Vizinhos, Paraná. The most commonly used plants were: Sage (Salvia officinalis), Herb-macaé (Leonuros sibiricus), breaking stone (Phyllanthus spp), Mallow (Malva sylvestris), Guaco (Mikania glomerata), Paripiroba (Piper umbellatum), cow foot (Bauhinia forficata), Balm white (Sedum dendroideum), Aloe (Aloe arborescens) Pennyroyal (Mentha pulegium), Mulberry leaf (Morus alba). The research concluded that medicinal herbs are widely used both preventively as curative.Eje A5: Sistemas de conocimientoFacultad de Ciencias Agrarias y Forestale

A psicologia como neurociência cognitiva : implicações para a compreensão dos processos básicos e suas aplicações

O presente artigo procura ilustrar o modo como os desenvolvimentos das neurociências cognitivas poderão ajudar a compreender alguns dos processos psicológicos básicos e, simultaneamente, ser traduzidos para importantes domínios da psicologia aplicada, particularmente no domínio clínico. Exemplificaremos a partir de algumas linhas de investigação programática em curso nas diferentes subsecções do Laboratório de Neuropsicofisiologia da Escola de Psicologia da Universidade do Minho. As potencialidades metodológicas proporcionadas pela neurofisiologia, neuroimagiologia, neuromodelação, psicofisiologia, neurobioquímica e neurogenética serão exemplificadas nas suas aplicações à linguagem (e suas implicações para a compreensão da esquizofrenia), funcionamento sócio-cognitivo (e implicações para a compreensão das perturbações do neurodesenvolvimento), funcionamento executivo (com implicações para a compreensão das perturbações do espectro obsessivo), empatia (e implicações para a compreensão da psicoterapia), mecanismos de stress (com implicações para a compreensão das perturbações de ansiedade), e, finalmente, comportamento animal (com implicações para o conhecimento dos sistemas sensoriais e perceptuais).(undefined

Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort

This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5 coding region, which is followed by Sanger population sequencing. NS5A RASs were present in 28.4% (23/81) of all GT1-infected patients with 9.9% (8/81) having the Y93C/H mutation. NS5B RASs showed a prevalence of 14.8% (12/81) and were only detected in GT1b. Overall 38.3% (31/81) of all GT1 HCV-infected patients presented baseline RASs. The obtained data supports the usefulness of resistance testing prior to treatment since a statistically significant association was found between treatment failure and the baseline presence of specific NS5 RASs known as Y93C/H (p = 0.04).publishersversionpublishe

Preparation of a Nanoemulsion with Carapa guianensis

Andiroba (Carapa guianensis) seeds are the source of an oil with a wide range of biological activities and ethnopharmacological uses. However, few studies have devoted attention to innovative formulations, including nanoemulsions. The present study aimed to obtain a colloidal system with the andiroba oil using a low-energy and organic-solvent-free method. Moreover, the preliminary residual larvicidal activity of the nanoemulsion against Aedes aegypti was evaluated. Oleic and palmitic acids were the major fatty acids, in addition to the phytosterol β-sitosterol and limonoids (tetranortriterpenoids). The required hydrophile-lipophile was around 11.0 and the optimal nanoemulsion was obtained using polysorbate 85. The particle size distribution suggested the presence of small droplets (mean diameter around 150 nm) and low polydispersity index (around 0.150). The effect of temperature on particle size distribution revealed that no major droplet size increase occurred. The preliminary residual larvicidal assay suggested that the mortality increased as a function of time. The present study allowed achievement of a potential bioactive oil in water nanoemulsion that may be a promising controlled release system. Moreover, the ecofriendly approach involved in the preparation associated with the great bioactive potential of C. guianensis makes this nanoemulsion very promising for valorization of this Amazon raw material

Effect of Nickel Nanocatalyst Loading on Supercritical Water Gasification of Coconut Shell

Impregnation of metal catalysts into biomass before thermochemical conversion may provide benefits of increased selective reactivity to obtain desirable products. In this work, coconut shells impregnated with increasing loadings of nickel were successfully prepared using a room-temperature impregnation method using a nickel salt solution at 1 and 2 molar (M) concentrations. The physicochemical characterization of the 2 M impregnated sample revealed the presence of 5.6 wt% of nickel with a particle size of 13.5 nm. The nickel-impregnated samples’ supercritical water gasification (SCWG) was conducted with biomass loading ranging from 20 wt% to 30 wt%, at temperatures between 400 °C and 500 °C, and residence times from 20 to 60 min. Higher nickel loading, higher temperatures and longer reaction times promoted the production of H2 and CO2 up to 15 and 79 mol%. Higher nickel loading also led to an increased Hydrogen Gasification Efficiency value of up to 133%. The analysis of hydrochars suggested that increasing nickel loading enhanced the reduction in nickel ions to the Ni0 nanoparticles, leading to higher H2. Additionally, the chemical composition of the liquid product showed the significant ability of nickel to promote lignin decomposition into phenol, facilitating the phenol hydrogenation reaction and subsequent gas production

Association of Candidate Gene Polymorphisms With Chronic Kidney Disease: Results of a Case-Control Analysis in the Nefrona Cohort

Chronic kidney disease (CKD) is a major risk factor for end-stage renal disease, cardiovascular disease and premature death. Despite classical clinical risk factors for CKD and some genetic risk factors have been identified, the residual risk observed in prediction models is still high. Therefore, new risk factors need to be identified in order to better predict the risk of CKD in the population. Here, we analyzed the genetic association of 79 SNPs of proteins associated with mineral metabolism disturbances with CKD in a cohort that includes 2, 445 CKD cases and 559 controls. Genotyping was performed with matrix assisted laser desorption ionizationtime of flight mass spectrometry. We used logistic regression models considering different genetic inheritance models to assess the association of the SNPs with the prevalence of CKD, adjusting for known risk factors. Eight SNPs (rs1126616, rs35068180, rs2238135, rs1800247, rs385564, rs4236, rs2248359, and rs1564858) were associated with CKD even after adjusting by sex, age and race. A model containing five of these SNPs (rs1126616, rs35068180, rs1800247, rs4236, and rs2248359), diabetes and hypertension showed better performance than models considering only clinical risk factors, significantly increasing the area under the curve of the model without polymorphisms. Furthermore, one of the SNPs (the rs2248359) showed an interaction with hypertension, being the risk genotype affecting only hypertensive patients. We conclude that 5 SNPs related to proteins implicated in mineral metabolism disturbances (Osteopontin, osteocalcin, matrix gla protein, matrix metalloprotease 3 and 24 hydroxylase) are associated to an increased risk of suffering CKD

Local hydrological conditions influence tree diversity and composition across the Amazon basin

Tree diversity and composition in Amazonia are known to be strongly determined by the water supplied by precipitation. Nevertheless, within the same climatic regime, water availability is modulated by local topography and soil characteristics (hereafter referred to as local hydrological conditions), varying from saturated and poorly drained to well-drained and potentially dry areas. While these conditions may be expected to influence species distribution, the impacts of local hydrological conditions on tree diversity and composition remain poorly understood at the whole Amazon basin scale. Using a dataset of 443 1-ha non-flooded forest plots distributed across the basin, we investigate how local hydrological conditions influence 1) tree alpha diversity, 2) the community-weighted wood density mean (CWM-wd) – a proxy for hydraulic resistance and 3) tree species composition. We find that the effect of local hydrological conditions on tree diversity depends on climate, being more evident in wetter forests, where diversity increases towards locations with well-drained soils. CWM-wd increased towards better drained soils in Southern and Western Amazonia. Tree species composition changed along local soil hydrological gradients in Central-Eastern, Western and Southern Amazonia, and those changes were correlated with changes in the mean wood density of plots. Our results suggest that local hydrological gradients filter species, influencing the diversity and composition of Amazonian forests. Overall, this study shows that the effect of local hydrological conditions is pervasive, extending over wide Amazonian regions, and reinforces the importance of accounting for local topography and hydrology to better understand the likely response and resilience of forests to increased frequency of extreme climate events and rising temperatures

Geographic patterns of tree dispersal modes in Amazonia and their ecological correlates

Aim: To investigate the geographic patterns and ecological correlates in the geographic distribution of the most common tree dispersal modes in Amazonia (endozoochory, synzoochory, anemochory and hydrochory). We examined if the proportional abundance of these dispersal modes could be explained by the availability of dispersal agents (disperser-availability hypothesis) and/or the availability of resources for constructing zoochorous fruits (resource-availability hypothesis). Time period: Tree-inventory plots established between 1934 and 2019. Major taxa studied: Trees with a diameter at breast height (DBH) ≥ 9.55 cm. Location: Amazonia, here defined as the lowland rain forests of the Amazon River basin and the Guiana Shield. Methods: We assigned dispersal modes to a total of 5433 species and morphospecies within 1877 tree-inventory plots across terra-firme, seasonally flooded, and permanently flooded forests. We investigated geographic patterns in the proportional abundance of dispersal modes. We performed an abundance-weighted mean pairwise distance (MPD) test and fit generalized linear models (GLMs) to explain the geographic distribution of dispersal modes. Results: Anemochory was significantly, positively associated with mean annual wind speed, and hydrochory was significantly higher in flooded forests. Dispersal modes did not consistently show significant associations with the availability of resources for constructing zoochorous fruits. A lower dissimilarity in dispersal modes, resulting from a higher dominance of endozoochory, occurred in terra-firme forests (excluding podzols) compared to flooded forests. Main conclusions: The disperser-availability hypothesis was well supported for abiotic dispersal modes (anemochory and hydrochory). The availability of resources for constructing zoochorous fruits seems an unlikely explanation for the distribution of dispersal modes in Amazonia. The association between frugivores and the proportional abundance of zoochory requires further research, as tree recruitment not only depends on dispersal vectors but also on conditions that favour or limit seedling recruitment across forest types