Plasticity with generalized hardening:Constitutive modeling and computational aspects

© Springer-Verlag Berlin Heidelberg 2016.In this work, an extended theory of plasticity with generalized hardening is proposed to describe the response of geomaterials under both mechanical and environmental processes, which include as special cases several elastoplastic constitutive equations proposed in the literature to model such processes as desaturation or suction hardening, thermal softening, chemo-mechanical coupling effects in fine-grained soils, as well as weathering of soft rocks. In the formulation of the theory, the coupling between mechanical and environmental processes takes place at two levels: first, as an additional direct contribution to the constitutive stress changes, taking place in both elastic and elastoplastic processes; and second, as a result of the evolution of the internal state variables induced by changes in the environmental process variables. This last effect is incorporated through a set of generalized hardening rules. As an example of application, the general formulation is specialized to the particular case of weak calcarenite rocks undergoing degradation processes due to plastic deformations, changes in degree of saturation (short-term debonding) and chemical dissolution of the bond material and the solid grains (long-term debonding). The resulting model is implemented in a FE code by means of an implicit generalized backward Euler algorithm, suitably modified to incorporate the full formalism of plasticity with generalized hardening. Results of numerical simulations carried out at the element level show the accuracy and efficiency properties of the proposed stresspoint algorithm. The simulation of a representative initialboundary value problem demonstrates the practical relevance of environmental degradation effects in practical applications, over periods of time comparable with the life cycle of most geotechnical structures

Microvesicles and Viral Infection▿

Cells secrete various membrane-enclosed microvesicles from their cell surface (shedding microvesicles) and from internal, endosome-derived membranes (exosomes). Intriguingly, these vesicles have many characteristics in common with enveloped viruses, including biophysical properties, biogenesis, and uptake by cells. Recent discoveries describing the microvesicle-mediated intercellular transfer of functional cellular proteins, RNAs, and mRNAs have revealed additional similarities between viruses and cellular microvesicles. Apparent differences include the complexity of viral entry, temporally regulated viral expression, and self-replication proceeding to infection of new cells. Interestingly, many virally infected cells secrete microvesicles that differ in content from their virion counterparts but may contain various viral proteins and RNAs. For the most part, these particles have not been analyzed for their content or functions during viral infection. However, early studies of microvesicles (L-particles) secreted from herpes simplex virus-infected cells provided the first evidence of microvesicle-mediated intercellular communication. In the case of Epstein-Barr virus, recent evidence suggests that this tumorigenic herpesvirus also utilizes exosomes as a mechanism of cell-to-cell communication through the transfer of signaling competent proteins and functional microRNAs to uninfected cells. This review focuses on aspects of the biology of microvesicles with an emphasis on their potential contributions to viral infection and pathogenesis