49 research outputs found
Nitric oxide triggers a transient metabolic reprogramming in Arabidopsis
[EN] Nitric oxide (NO) regulates plant growth and development as well as responses to stress that enhanced its endogenous production. Arabidopsis plants exposed to a pulse of exogenous NO gas were used for untargeted global metabolomic analyses thus allowing the identification of metabolic processes affected by NO. At early time points after treatment, NO scavenged superoxide anion and induced the nitration and the S-nitrosylation of proteins. These events preceded an extensive though transient metabolic reprogramming at 6 h after NO treatment, which included enhanced levels of polyamines, lipid catabolism and accumulation of phospholipids, chlorophyll breakdown, protein and nucleic acid turnover and increased content of sugars. Accordingly, lipid-related structures such as root cell membranes and leaf cuticle altered their permeability upon NO treatment. Besides, NO-treated plants displayed degradation of starch granules, which is consistent with the increased sugar content observed in the metabolomic survey. The metabolic profile was restored to baseline levels at 24 h post-treatment, thus pointing up the plasticity of plant metabolism in response to nitroxidative stress conditions.This work was supported by grants BIO2011-27526 and BIO2014-56067-P from the Spanish Ministry of Economy and Competitiveness and FEDER funds. We thank support and comments from Danny Alexander (Metabolon Inc., USA) on metabolomic analyses.Leon Ramos, J.; Costa-Broseta, Á.; Castillo López Del Toro, MC. (2016). Nitric oxide triggers a transient metabolic reprogramming in Arabidopsis. Scientific Reports. 6:1-14. doi:10.1038/srep37945S1146Arc, E., Galland, M., Godin, B., Cueff, G. & Rajjou, L. Nitric oxide implication in the control of seed dormancy and germination. Front. Plant Sci. 4, 346 (2013).Beligni, M. V. & Lamattina, L. 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Response of barley seedlings to water deficit and enhanced UV-B irradiation acting alone and in combination
Diverse functional interactions between nitric oxide and abscisic acid in plant development and responses to stress
[EN] The extensive support for abscisic acid (ABA) involvement in the complex regulatory networks controlling stress
responses and development in plants contrasts with the relatively recent role assigned to nitric oxide (NO). Because
treatment with exogenous ABA leads to enhanced production of NO, it has been widely considered that NO participates
downstream of ABA in controlling processes such as stomata movement, seed dormancy, and germination.
However, data on leaf senescence and responses to stress suggest that the functional interaction between ABA and
NO is more complex than previously thought, including not only cooperation but also antagonism. The functional
relationship is probably determined by several factors including the time- and place-dependent pattern of accumulation
of both molecules, the threshold levels, and the regulatory factors important for perception. These factors will
determine the actions exerted by each regulator. Here, several examples of well-documented functional interactions
between NO and ABA are analysed in light of the most recent reported data on seed dormancy and germination, stomata
movements, leaf senescence, and responses to abiotic and biotic stressesThis work was supported by MICINN (Spain) grants BIO2011-27526 and CONSOLIDER CSD2007-00057 to JL and postdoctoral contracts to MCC and AC. RM was funded by a pre-doctoral fellowship of the FPU Program from MEC (Spain). We thank Michael Holdsworth (University of Nottingham, UK) for critical reading of this manuscript and for his helpful comments and corrections. We would also like to express our appreciation for all the contributions reported in this research area, and particularly to those researchers who have not been cited in this review because of the limitations of manuscript length.Leon Ramos, J.; Castillo López Del Toro, MC.; Coego González, A.; Lozano Juste, J.; Mir Moreno, R. (2014). Diverse functional interactions between nitric oxide and abscisic acid in plant development and responses to stress. Journal of Experimental Botany. 65(4):907-921. https://doi.org/10.1093/jxb/ert454S907921654ALBORESI, A., GESTIN, C., LEYDECKER, M.-T., BEDU, M., MEYER, C., & TRUONG, H.-N. (2005). Nitrate, a signal relieving seed dormancy in Arabidopsis. Plant, Cell and Environment, 28(4), 500-512. doi:10.1111/j.1365-3040.2005.01292.xAsai, S., & Yoshioka, H. (2009). Nitric Oxide as a Partner of Reactive Oxygen Species Participates in Disease Resistance to Necrotrophic Pathogen Botrytis cinerea in Nicotiana benthamiana. Molecular Plant-Microbe Interactions, 22(6), 619-629. doi:10.1094/mpmi-22-6-0619Asselbergh, B., De Vleesschauwer, D., & Höfte, M. (2008). Global Switches and Fine-Tuning—ABA Modulates Plant Pathogen Defense. Molecular Plant-Microbe Interactions, 21(6), 709-719. doi:10.1094/mpmi-21-6-0709Batak, I., Dević, M., Gibal, Z., Grubišić, D., Poff, K. L., & Konjević, R. (2002). The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds. Seed Science Research, 12(4), 253-259. doi:10.1079/ssr2002118Beligni, M. V., & Lamattina, L. (2000). Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants. Planta, 210(2), 215-221. doi:10.1007/pl00008128Bellin, D., Asai, S., Delledonne, M., & Yoshioka, H. (2013). Nitric Oxide as a Mediator for Defense Responses. Molecular Plant-Microbe Interactions, 26(3), 271-277. doi:10.1094/mpmi-09-12-0214-crBethke, P. C., Libourel, I. G. L., Aoyama, N., Chung, Y.-Y., Still, D. W., & Jones, R. L. (2007). The Arabidopsis Aleurone Layer Responds to Nitric Oxide, Gibberellin, and Abscisic Acid and Is Sufficient and Necessary for Seed Dormancy. Plant Physiology, 143(3), 1173-1188. doi:10.1104/pp.106.093435Bethke, P. C., Libourel, I. G. L., & Jones, R. L. (2005). Nitric oxide reduces seed dormancy in Arabidopsis. Journal of Experimental Botany, 57(3), 517-526. doi:10.1093/jxb/erj060Bethke, P. C., Libourel, I. G. L., Reinöhl, V., & Jones, R. L. (2005). Sodium nitroprusside, cyanide, nitrite, and nitrate break Arabidopsis seed dormancy in a nitric oxide-dependent manner. Planta, 223(4), 805-812. doi:10.1007/s00425-005-0116-9Bright, J., Desikan, R., Hancock, J. T., Weir, I. S., & Neill, S. J. (2005). ABA-induced NO generation and stomatal closure in Arabidopsis are dependent on H2
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