Blended Learning: Integrating an e-Learning Component for Competency Validation

Introduction: Emergency care providers are required to demonstrate competency in the management of life-threatening situation. The care provider’s ability to manage an emergency situation depends upon his/her knowledge and skills in basic CPR; and the use of emergency equipment and supplies. The education department at our healthcare facility is responsible for CPR/Emergency Management competency validation of over 2500 employees annually. Historically each employee was scheduled to attend 4 hours of class every year to review the content, complete the post-test and demonstrate skills. It was resource-intensive, time consuming, stressful and often difficult to schedule the 24/7 employees for the sessions. [See PDF for complete abstract

Label-Free Metabolic Classification of Single Cells in Droplets Using the Phasor Approach to Fluorescence Lifetime Imaging Microscopy.

Characterization of single cell metabolism is imperative for understanding subcellular functional and biochemical changes associated with healthy tissue development and the progression of numerous diseases. However, single-cell analysis often requires the use of fluorescent tags and cell lysis followed by genomic profiling to identify the cellular heterogeneity. Identifying individual cells in a noninvasive and label-free manner is crucial for the detection of energy metabolism which will discriminate cell types and most importantly critical for maintaining cell viability for further analysis. Here, we have developed a robust assay using the droplet microfluidic technology together with the phasor approach to fluorescence lifetime imaging microscopy to study cell heterogeneity within and among the leukemia cell lines (K-562 and Jurkat). We have extended these techniques to characterize metabolic differences between proliferating and quiescent cells-a critical step toward label-free single cancer cell dormancy research. The result suggests a droplet-based noninvasive and label-free method to distinguish individual cells based on their metabolic states, which could be used as an upstream phenotypic platform to correlate with genomic statistics. © 2018 International Society for Advancement of Cytometry

Rapid and label-free identification of single leukemia cells from blood in a high-density microfluidic trapping array by fluorescence lifetime imaging microscopy.

The rapid screening and isolation of single leukemia cells from blood has become critical for early leukemia detection and tumor heterogeneity interrogation. However, due to the size overlap between leukemia cells and the more abundant white blood cells (WBCs), the isolation and identification of leukemia cells individually from peripheral blood is extremely challenging and often requires immunolabeling or cytogenetic assays. Here we present a rapid and label-free single leukemia cell identification platform that combines: (1) high-throughput size-based separation of hemocytes via a single-cell trapping array, and (2) leukemia cell identification through phasor approach and fluorescence lifetime imaging microscopy (phasor-FLIM), to quantify changes between free/bound nicotinamide adenine dinucleotide (NADH) as an indirect measurement of metabolic alteration in living cells. The microfluidic trapping array designed with 1600 highly-packed addressable single-cell traps can simultaneously filter out red blood cells (RBCs) and trap WBCs/leukemia cells, and is compatible with low-magnification imaging and fast-speed fluorescence screening. The trapped single leukemia cells, e.g., THP-1, Jurkat and K562 cells, are distinguished from WBCs in the phasor-FLIM lifetime map, as they exhibit significant shift towards shorter fluorescence lifetime and a higher ratio of free/bound NADH compared to WBCs, because of their glycolysis-dominant metabolism for rapid proliferation. Based on a multiparametric scheme comparing the eight parameter-spectra of the phasor-FLIM signatures, spiked leukemia cells are quantitatively distinguished from normal WBCs with an area-under-the-curve (AUC) value of 1.00. Different leukemia cell lines are also quantitatively distinguished from each other with AUC values higher than 0.95, demonstrating high sensitivity and specificity for single cell analysis. The presented platform is the first to enable high-density size-based single-cell trapping simultaneously with RBC filtering and rapid label-free individual-leukemia-cell screening through non-invasive metabolic imaging. Compared to conventional biomolecular diagnostics techniques, phasor-FLIM based single-cell screening is label-free, cell-friendly, robust, and has the potential to screen blood in clinical volumes through parallelization

Properties and Performance of Two Wide Field of View Cherenkov/Fluorescence Telescope Array Prototypes

A wide field of view Cherenkov/fluorescence telescope array is one of the main components of the Large High Altitude Air Shower Observatory project. To serve as Cherenkov and fluorescence detectors, a flexible and mobile design is adopted for easy reconfiguring of the telescope array. Two prototype telescopes have been constructed and successfully run at the site of the ARGO-YBJ experiment in Tibet. The features and performance of the telescopes are presented

Pengaruh Latar Belakang Profesi Terhadap Persepsi Mengenai Kerjasama Interprofesi

AbstrakAnggota tim profesi kesehatan diharuskan bekerjasama dengan sesama anggotadalam menciptakan layanan kesehatan terpadu dan mewujudkan layanan yangterbaik untuk kesehatan dan keselamatan pasien. Isu kurangnya kerjasama antarprofesi kesehatan perlu diminimalisir dengan memaparkan edukasi interprofesisedini mungkin. Tujuan penelitian ini mengetahui pengaruh latar belakangpendidikan terhadap persepsi mengenai kerjasama interprofesi. Penelitian analitikobservasional dengan rancangan post-test design. Subjek penelitian yaitumahasiswa sarjana kedokteran, farmasi, dan keperawatan yang berjumlah total 288orang. IPE dilaksanakan pada modul respirasi dengan kegiatan diskusi kelompokkecil (SGD) dan skill lab masing-masing 2 kali selama 5 hari. Kompetensi kerjasamainterprofesi diukur dengan kuesioner Interprofessional Teamwork Evaluationsetelah mengikuti IPE. Perbedaan pengaruh latar belakang profesi terhadappersepsi mengenai IPT dianalisis dengan uji Kruskal-Wallis. Tidak ada perbedaansignifikan skor Interprofessional Teamwork Evaluation per domain (Pstruktur tim =0,95; Pkepemimpinan = 0,81; Pmonitoring situasi = 0,43; Pdukungan kelompok = 0,75; Pkomunikasi = 0,57) pada prodi sarjana kedokteran, farmasi dan keperawatan setelah mengikuti IPE modul respirasi. Pembagian peran dan hubungan secara horizontal dan vertikal memfasilitasi struktur tim dalam kerjasama interprofesi. Perbedaan latar belakang profesi anggota tim membangun persepsi mahasiswa dukungan kelompok dalam penyelesaian tugas. Adanya hubungan vertikal yang dinamis dan koordinasi tim mendukung persepsi terhadap kepemimpinan. Persamaan persepsi terhadap komunikasi didorong oleh anggota tim dalam memecahkan masalah dan mencapai tujuan bersama. Koordinasi dan proses feedback yang aktif disertai struktur tim yang kuat mendukung persamaan persepsi monitoring situasi. Secara keseluruhan, tidak ada perbedaan persepsi kerjasama interprofesi antara sarjana kedokteran, farmasi, dan keperawatan setelah mengikuti kegiatan IPE.Kata Kunci: utama kerjasama interprofesi, persepsi, edukasi interprofesi

Critical Exponents of the Metal-Insulator Transition in the Two-Dimensional Hubbard Model

We study the filling-controlled metal-insulator transition in the two-dimensional Hubbard model near half-filling with the use of zero temperature quantum Monte Carlo methods. In the metallic phase, the compressibility behaves as $\kappa \propto |\mu - \mu_c|^{-0.58\pm0.08}$ where $\mu_c$ is the critical chemical potential. In the insulating phase, the localization length follows $\xi_l \propto |\mu - \mu_c|^{-\nu_l}$ with $\nu_l = 0.26 \pm 0.05$. Under the assumption of hyperscaling, the compressibility data leads to a correlation length exponent $\nu_\kappa = 0.21 \pm 0.04$. Our results show that the exponents $\nu_\kappa$ and $\nu_l$ agree within statistical uncertainty. This confirms the assumption of hyperscaling with correlation length exponent $\nu = 1/4$ and dynamical exponent $z = 4$. In contrast the metal-insulator transition in the generic band insulators in all dimensions as well as in the one-dimensional Hubbard model satisfy the hyperscaling assumption with exponents $\nu = 1/2$ and $z = 2$.Comment: Two references added. The DVI file and PS figure files are also available at http://www.issp.u-tokyo.ac.jp/labs/riron/imada/furukawa/; to appear in J. Phys. Soc. Jpn 65 (1996) No.

Scanning Tunneling Microscopy and Tunneling Luminescence of the Surface of GaN Films Grown by Vapor Phase Epitaxy

We report scanning tunneling microscopy (STM) images of surfaces of GaN films and the observation of luminescence from those films induced by highly spatially localized injection of electrons or holes using STM. This combination of scanning tunneling luminescence (STL) with STM for GaN surfaces and the ability to observe both morphology and luminescence in GaN is the first step to investigate possible correlations between surface morphology and optical properties.Comment: 12 pages, Revtex 3.0, submitted to Appl. Phys. Lett., three figures available from Jian Ma at [email protected]

Effect of hydrogen bonding and complexation with metal ions on the fluorescence of luotonin A

Fluorescence characteristics of a biologically active natural alkaloid, luotonin A (LuA), were studied by steady-state and time-resolved spectroscopic methods. The rate constant of the radiationless deactivation from the singlet-excited state diminished by more than one order of magnitude when the solvent polarity was changed from toluene to water. Dual emission was found in polyfluorinated alcohols of large hydrogen bond donating ability due to photoinitiated proton displacement along the hydrogen bond. In CH 2Cl2, LuA produced both 1:1 and 1:2 hydrogen-bonded complexes with hexafluoro-2-propanol (HFIP) in the ground state. Photoexcitation of the 1:2 complex led to protonated LuA, whose fluorescence appeared at a long wavelength. LuA served as a bidentate ligand forming 1:1 complexes with metal ions in acetonitrile. The stability of the complexes diminished in the series of Cd2+ > Zn2+ > Ag+, and upon competitive binding of water to the metal cations. The effect of chelate formation on the fluorescent properties was revealed. © 2013 The Royal Society of Chemistry and Owner Societies

Recovery of the persistent current induced by the electron-electron interaction in mesoscopic metallic rings

Persistent currents in mesoscopic metallic rings induced by static magnetic fields are investigated by means of a Hamiltonian which incorporates diagonal disorder and the electron-electron interaction through a Hubbard term ($U$). Correlations are included up to second order perturbation theory which is shown to work accurately for $U$ of the order of the hopping integral. If disorder is not very strong, interactions increase the current up to near its value for a clean metal. Averaging over ring lengths eliminates the first Fourier component of the current and reduces its value, which remains low after interactions are included.Comment: uuencoded gzipped tar file containing the manuscript (tex file) and four figures (postscript files). Accepted for publication in Solid State Communications. Send e-mail to: [email protected]