Control Natural Language Query Editor Guided by OWL/XML Path

It is become difficult when common user try to access semantic web application or any one ontology database-like gene ontology, plant ontology etc. Because either he/she don’t know about ontology technology or about no of concepts present in the ontology database. So only expert user can handle the applications or database. To avoid this problem, I have developed the Controlled natural language query editor guided by xml path. Here ontology data is get used in the xml file format and then that file is get used to generate ontology tree. Ontology tree is work as suggester for user in the generation of their query. Query then fire on xml/ontology file to retrieve desire result

Molecular study of Anaplasma marginale parasite in carrier cattle in Al-Nasiriyah city

To detect Anaplasma marginale among carrier cattle by using polymerase chain reaction (PCR) technique, 64 blood samples, fromhealthy cows in abattoir of Al-Nasiriyah city were collected from June till August, 2017 in this study. By targeting MAR1bB2 gene with the molecular weight of approximately 265 bp, Anaplasma marginale were detected in18 samples (28.125%). One of these positive sample was recoded in National Center for Biotechnology Information, NCBI; Gene Bank

Timing the initiation of multiple myeloma

The evolution and progression of multiple myeloma and its precursors over time is poorly understood. Here, we investigate the landscape and timing of mutational processes shaping multiple myeloma evolution in a large cohort of 89 whole genomes and 973 exomes. We identify eight processes, including a mutational signature caused by exposure to melphalan. Reconstructing the chronological activity of each mutational signature, we estimate that the initial transformation of a germinal center B-cell usually occurred during the first 2nd-3rd decades of life. We define four main patterns of activation-induced deaminase (AID) and apolipoprotein B mRNA editing catalytic polypeptide-like (APOBEC) mutagenesis over time, including a subset of patients with evidence of prolonged AID activity during the pre-malignant phase, indicating antigen-responsiveness and germinal center reentry. Our findings provide a framework to study the etiology of multiple myeloma and explore strategies for prevention and early detection

Transcriptional and Post-transcriptional Gene Regulation by Long Non-coding RNA.

Advances in genomics technology over recent years have led to the surprising discovery that the genome is far more pervasively transcribed than was previously appreciated. Much of the newly-discovered transcriptome appears to represent long non-coding RNA (lncRNA), a heterogeneous group of largely uncharacterised transcripts. Understanding the biological function of these molecules represents a major challenge and in this review we discuss some of the progress made to date. One major theme of lncRNA biology seems to be the existence of a network of interactions with microRNA (miRNA) pathways. lncRNA has been shown to act as both a source and an inhibitory regulator of miRNA. At the transcriptional level, a model is emerging whereby lncRNA bridges DNA and protein by binding to chromatin and serving as a scaffold for modifying protein complexes. Such a mechanism can bridge promoters to enhancers or enhancer-like non-coding genes by regulating chromatin looping, as well as conferring specificity on histone modifying complexes by directing them to specific loci

Impact of chronic stress protocols in learning and memory in rodents: systematic review and meta-analysis

The idea that maladaptive stress impairs cognitive function has been a cornerstone of decades in basic and clinical research. However, disparate findings have reinforced the need to aggregate results from multiple sources in order to confirm the validity of such statement. In this work, a systematic review and meta-analyses were performed to aggregate results from rodent studies investigating the impact of chronic stress on learning and memory. Results obtained from the included studies revealed a significant effect of stress on global cognitive performance. In addition, stressed rodents presented worse consolidation of learned memories, although no significantly differences between groups at the acquisition phase were found. Despite the methodological heterogeneity across studies, these effects were independent of the type of stress, animals' strains or age. However, our findings suggest that stress yields a more detrimental effect on spatial navigation tests' performance. Surprisingly, the vast majority of the selected studies in this field did not report appropriate statistics and were excluded from the quantitative analysis. We have therefore purposed a set of guidelines termed PROBE (Preferred Reporting Orientations for Behavioral Experiments) to promote an adequate reporting of behavioral experiments.This work was funded by the European Commission (FP7) "SwitchBox" (Contract HEALTH-F2-2010-259772) project and co-financed by the Portuguese North Regional Operational Program (ON.2 - O Novo Norte) under the National Strategic Reference Framework (QREN), through the European Regional Development Fund (FEDER), and by Fundacao Calouste Gulbenkian (Portugal) (Contract grant number: P-139977; project "Better mental health during ageing based on temporal prediction of individual brain ageing trajectories (TEMPO)"). PSM is supported by an FCT fellowship grant, from the PhD-iHES program, with the reference PDE/BDE/113601/2015.info:eu-repo/semantics/publishedVersio

Studies on catalytic co-pyrolysis of bakelite and refineries residual fuel oil using ZSM-5 catalyst to produce lighter fuel oil

426-430Refineries Residual Fuel Oil (RFO) and Bakelite (BL) and were co-pyrolyzed together using catalyst ZSM-5 under the atmospheric pressure with different mixing ratios of the feedstocks. The TGA analysis shows that most of the degradation take place between the temperature range of 250-400oC. The studies were carried out at two different temperatures of 500oC and 600oC using a batch reactor with different blended ratios, with blended feedstocks to catalyst ratio of 4:1. The effects of the two variables i.e. temperature and blending ratios among the two feedstocks, in terms of the products yield of liquid fuel, gas, coke and conversion were determined. The blending ratio of 4:1 (RFO: BL) yielding highest conversion of 76 wt. % at 600oC was found to be the most favourable condition in this investigation

DIFFERENT EFFECTS OF TIANEPTINE PRETREATMENT IN RATS EXPOSED TO ACUTE STRESS AND REPEATED SEVERE STRESS

In this study we aim to discuss the relationship between stress and learning and emotionality in on experimental model using two different stress conditions: acute stress (single restraint stress for 20 min) and repeated severe stress (6-h doily restraint for 27 days). We studied the effects of tianeptine, which has been suggested to hove anxiolytic and cognition-enhancing effects under stressful conditions. After acute stress, the increase in the duration of immobility (F = 5.753 and 3.664) in the open field and holeboard tests and the decrease in rearing (F = 3.891) in the holeboard test were significant when compared to controls (P < 0.05). Results for repeated severe stress showed that in both the open field and holeboard tests the decrease in rearing (F 4.494 and 4.530, respectively), increase in the duration of immobility (F = 6.069 and 4.742, respectively) and decrease in head dips (F 4,938) in the holeboard test were statistically significant (P < 0.05). The group pretreated with tianeptine showed no significant difference from controls for either acute or repeated severe stress conditions. In the Morris water maze test, acute stress led to a prolongation of average escape latency, which indicated a spatial learning deficit. Treatment with tianeptine prior to acute stress prevented this spatial deficit. Repeated severe stress also led to spatial learning deficits in rats but this deficit was not prevented by treatment with tianeptine. Our study demonstrates that pretreatment with tianeptine had different effects on stress-induced spatial learning deficits under acute and repeated stress conditions, while the effects on emotionality and anxiety-like behavior were similar The mechanisms implicated in stress-induced emotional and memory deficits will be discussed