649 research outputs found
Model for eukaryotic tail-anchored protein binding based on the structure of Get3
The Get3 ATPase directs the delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (ER). TA-proteins are characterized by having a single transmembrane helix (TM) at their extreme C terminus and include many essential proteins, such as SNAREs, apoptosis factors, and protein translocation components. These proteins cannot follow the SRP-dependent co-translational pathway that typifies most integral membrane proteins; instead, post-translationally, these proteins are recognized and bound by Get3 then delivered to the ER in the ATP dependent Get pathway. To elucidate a molecular mechanism for TA protein binding by Get3 we have determined three crystal structures in apo and ADP forms from Saccharomyces cerevisae (ScGet3-apo) and Aspergillus fumigatus (AfGet3-apo and AfGet3-ADP). Using structural information, we generated mutants to confirm important interfaces and essential residues. These results point to a model of how Get3 couples ATP hydrolysis to the binding and release of TA-proteins
Excitation function of elliptic flow in Au+Au collisions and the nuclear matter equation of state
We present measurements of the excitation function of elliptic flow at
midrapidity in Au+Au collisions at beam energies from 0.09 to 1.49 GeV per
nucleon. For the integral flow, we discuss the interplay between collective
expansion and spectator shadowing for three centrality classes. A complete
excitation function of transverse momentum dependence of elliptic flow is
presented for the first time in this energy range, revealing a rapid change
with incident energy below 0.4 AGeV, followed by an almost perfect scaling at
the higher energies. The equation of state of compressed nuclear matter is
addressed through comparisons to microscopic transport model calculations.Comment: 10 pages, 4 eps figures, submitted for publication. Data files will
be available at http://www.gsi.de/~fopiwww/pub
Effects of nonâsteroidal antiâinflammatory drugs and other eicosanoid pathway modifiers on antiviral and allergic responses. EAACI task force on eicosanoids consensus report in times of COVIDâ19
Nonâsteroidal antiâinflammatory drugs (NSAIDs) and other eicosanoid pathway modifiers are among the most ubiquitously used medications in the general population. Their broad antiâinflammatory, antipyretic, and analgesic effects are applied against symptoms of respiratory infections, including SARSâCoVâ2, as well as in other acute and chronic inflammatory diseases that often coexist with allergy and asthma. However, the current pandemic of COVIDâ19 also revealed the gaps in our understanding of their mechanism of action, selectivity, and interactions not only during viral infections and inflammation, but also in asthma exacerbations, uncontrolled allergic inflammation, and NSAIDsâexacerbated respiratory disease (NERD). In this context, the consensus report summarizes currently available knowledge, novel discoveries, and controversies regarding the use of NSAIDs in COVIDâ19, and the role of NSAIDs in asthma and viral asthma exacerbations. We also describe here novel mechanisms of action of leukotriene receptor antagonists (LTRAs), outline how to predict responses to LTRA therapy and discuss a potential role of LTRA therapy in COVIDâ19 treatment. Moreover, we discuss interactions of novel T2 biologicals and other eicosanoid pathway modifiers on the horizon, such as prostaglandin D2 antagonists and cannabinoids, with eicosanoid pathways, in context of viral infections and exacerbations of asthma and allergic diseases. Finally, we identify and summarize the major knowledge gaps and unmet needs in current eicosanoid research
Tracing the Evolution of Temperature in Near Fermi Energy Heavy Ion Collisions
The kinetic energy variation of emitted light clusters has been employed as a
clock to explore the time evolution of the temperature for thermalizing
composite systems produced in the reactions of 26A, 35A and 47A MeV Zn
with Ni, Mo and Au. For each system investigated, the
double isotope ratio temperature curve exhibits a high maximum apparent
temperature, in the range of 10-25 MeV, at high ejectile velocity. These
maximum values increase with increasing projectile energy and decrease with
increasing target mass. The time at which the maximum in the temperature curve
is reached ranges from 80 to 130 fm/c after contact. For each different target,
the subsequent cooling curves for all three projectile energies are quite
similar. Temperatures comparable to those of limiting temperature systematics
are reached 30 to 40 fm/c after the times corresponding to the maxima, at a
time when AMD-V transport model calculations predict entry into the final
evaporative or fragmentation stage of de-excitation of the hot composite
systems. Evidence for the establishment of thermal and chemical equilibrium is
discussed.Comment: 9 pages, 5 figure
Isotopic Dependence of the Nuclear Caloric Curve
The A/Z dependence of projectile fragmentation at relativistic energies has
been studied with the ALADIN forward spectrometer at SIS. A stable beam of
124Sn and radioactive beams of 124La and 107Sn at 600 MeV per nucleon have been
used in order to explore a wide range of isotopic compositions. Chemical
freeze-out temperatures are found to be nearly invariant with respect to the
A/Z of the produced spectator sources, consistent with predictions for expanded
systems. Small Coulomb effects (\Delta T \approx 0.6 MeV) appear for residue
production near the onset of multifragmentation.Comment: 11 pages, 3 figures, accepted for publ. in Phys. Rev. Let
Tracing a phase transition with fluctuations of the largest fragment size: Statistical multifragmentation models and the ALADIN S254 data
A phase transition signature associated with cumulants of the largest
fragment size distribution has been identified in statistical
multifragmentation models and examined in analysis of the ALADIN S254 data on
fragmentation of neutron-poor and neutron-rich projectiles. Characteristics of
the transition point indicated by this signature are weakly dependent on the
A/Z ratio of the fragmenting spectator source. In particular, chemical
freeze-out temperatures are estimated within the range 5.9 to 6.5 MeV. The
experimental results are well reproduced by the SMM model.Comment: 7 pages, 3 figures, Proceedings of the International Workshop on
Multifragmentation and Related Topics (IWM2009), Catania, Italy, November
2009
- âŠ