The effects of a mindfulness-based family psychoeducation intervention for the caregivers of young adults with first-episode psychosis: A randomized controlled trial

Objective: In this study, we investigated the effects of a mindfulness-based family psychoeducation (MBFPE) program on the mental-health outcomes of both caregivers and young adults with first-episode psychosis with an onset in the past three years through a multi-site randomized controlled trial. We also studied the outcomes of three potential mediating effects of interpersonal mindfulness, expressed emotions, and non-attachment on the program. Method: We randomly assigned 65 caregivers of young adults with psychosis to MBFPE (n = 33) or an ordinary family psychoeducation (FPE) program (n = 32); among them, 18 young adults in recovery also participated in the evaluation of outcomes. Results: Intent-to-treat analyses were conducted. No significant time × group interaction effects of MBFPE and FPE programs were found in any of the caregivers’ outcomes. However, the young adults with psychosis reported higher levels of recovery after the MBFPE program than after the ordinary FPE program (F = 8.268, p = 0.012, d = 1.484). They also reported a larger reduction in over-involvement of their caregivers (F = 4.846, p = 0.044, d = 1.136), showing that MBFPE had a superior effect to FPE in promoting recovery and reducing over-involvement. Conclusions: A brief psychoeducation program may not reduce the burden on or improve the mental-health outcome of caregivers of individuals with recent-onset psychosis. However, integrating mindfulness into a conventional family psychoeducation program may reduce the expressed emotions of caregivers, especially over-involvement. Further studies should explore how psychoeducation programs can reduce the impact of psychosis on family through sustainable effects in terms of reducing their burden and expressed emotions, using a rigorous study and adequate sample size

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Measurement of b jet shapes in proton-proton collisions at root s=5.02 TeV

We present the first study of charged-hadron production associated with jets originating from b quarks in proton-proton collisions at a center-of-mass energy of 5.02 TeV. The data sample used in this study was collected with the CMS detector at the CERN LHC and corresponds to an integrated luminosity of 27.4 pb(-1). To characterize the jet substructure, the differential jet shapes, defined as the normalized transverse momentum distribution of charged hadrons as a function of angular distance from the jet axis, are measured for b jets. In addition to the jet shapes, the per-jet yields of charged particles associated with b jets are also quantified, again as a function of the angular distance with respect to the jet axis. Extracted jet shape and particle yield distributions for b jets are compared with results for inclusive jets, as well as with the predictions from the pythia and herwig++ event generators.Peer reviewe

Measurement of the azimuthal anisotropy of Y(1S) and Y(2S) mesons in PbPb collisions at root s(NN)=5.02 TeV

The second-order Fourier coefficients (v(2)) characterizing the azimuthal distributions of Y(1S) and Y(2S) mesons produced in PbPb collisions at root s(NN) = 5.02 TeV are studied. The Y mesons are reconstructed in their dimuon decay channel, as measured by the CMS detector. The collected data set corresponds to an integrated luminosity of 1.7 nb(-1). The scalar product method is used to extract the v2 coefficients of the azimuthal distributions. Results are reported for the rapidity range vertical bar y vertical bar < 2.4, in the transverse momentum interval 0 < pT < 50 GeV/c, and in three centrality ranges of 10-30%, 30-50% and 50-90%. In contrast to the J/psi mesons, the measured v(2) values for the Y mesons are found to be consistent with zero. (C) 2021 The Author(s). Published by Elsevier B.V.Peer reviewe

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Measurement of quark- and gluon-like jet fractions using jet charge in PbPb and pp collisions at 5.02 TeV

The momentum-weighted sum of the electric charges of particles inside a jet, known as jet charge, is sensitive to the electric charge of the particle initiating the parton shower. This paper presents jet charge distributions in $\sqrt{s_{\mathrm{NN}}}$ = 5.02 TeV lead-lead (PbPb) and proton-proton (pp) collisions recorded with the CMS detector at the LHC. These data correspond to integrated luminosities of 404 μb$^{−1}$ and 27.4 pb$^{−1}$ for PbPb and pp collisions, respectively. Leveraging the sensitivity of the jet charge to fundamental differences in the electric charges of quarks and gluons, the jet charge distributions from simulated events are used as templates to extract the quark- and gluon-like jet fractions from data. The modification of these jet fractions is examined by comparing pp and PbPb data as a function of the overlap of the colliding Pb nuclei (centrality). This measurement tests the color charge dependence of jet energy loss due to interactions with the quark-gluon plasma. No significant modification between different centrality classes and with respect to pp results is observed in the extracted quark- and gluon-like jet fractions

Study of excited Lambda(0)(b) states decaying to Lambda(0)(b)pi(+)pi(-) in proton-proton collisions at root s=13TeV

A study of excited Lambda(0)(b) baryons is reported, based on a data sample collected in 2016-2018 with the CMS detector at the LHC in proton-proton collisions at a center-of-mass energy of 13 TeV, corresponding to an integrated luminosity of up to 140fb(-1). The existence of four excited Lambda(0)(b) states: Lambda(0)(b) (5912)(0), Lambda(0)(b) (5920)(0), Lambda(0)(b) (6146)(0), and Lambda(0)(b) (6152)(0) in the Lambda(0)(b)pi(+)pi(-) mass spectrum is confirmed, and their masses are measured. The Lambda(0)(b)pi(+)pi(-) mass distribution exhibits a broad excess of events in the region of 6040-6100 MeV, whose origin cannot be discerned with the present data. (C) 2020 The Author(s). Published by Elsevier B.V

The production of isolated photons in PbPb and pp collisions at sNN=\sqrt{s_\mathrm{NN}} = 5.02 TeV

The combination between petrological data and historical documentation sources is necessary to establish an accurate methodology for the location of the original quarries of the stony materials used in a determinate historical building. The petrographical and petrophisical data correlation combined with a multivariate statistical analysis, by means of similarity indexes of both the materials in the building and the probable source geological formations, allows the location even when the documental sources would he incomplete or lost, or when the source areas would be modified. The works of location of original quarries used in architectural heritage are important since they allow improving the works of restoration on a building by means of the possibility of using identical materials for substitution or reintegration of decayed elements. The establishment of the original areas also offers the possibility of obtaining unaltered semipiés to evaluate the state and processes of decay of the building as well as to use this rough material to test the effectivity and durability of protective arid consolidant treatments in the building and monuments. The aim of this work has been to characterize and to locate the origin of the stony material in the stonework of the Crypt of the Cathedral of Santa María la Real de la Almudena (Madrid). These stone types came from quarries located in Viana de Jadraque (Guadalajara, Spain) and in the central area of Portugal. These materials do not correspond with the traditional ones used in the city of Madrid and its presence is due to the opening of new ways of transport in the second half of the 19"' centuiy.Para establecer una metodología adecuada con el fin de localizar las canteras originales de los materiales pétreos de construcción utilizados en un determinado edificio histórico se necesita combinar datos petrológicos y fuentes de documentación histórica. La correlación de los datos petrográficos y petrofísicos combinada con el análisis estadístico multivariante, mediante índices de similitud entre el edificio y las probables formaciones geológicas fuente, permite la localizacion si las fuentes documentales se hubiesen perdido o fuesen incompletas o si las áreas de extracción han sido modificadas. La localizacion de los materiales originales es importante porque permite mejorar las labores de restauración del edificio al poder utilizar un material idéntico para sustitución y reintegración de elementos deteriorados. También ofrece la posibilidad de obtener muestras para evaluar los procesos y estado de deterioro del edificio y utilizar este material para los ensayos de efectividad y durabilidad de los tratamientos de consolidación y protección del monumento. El objetivo de este trabajo ha sido caracterizar y localizar el origen de los materiales pétreos de la sillería de la Cripta de la Catedral de Santa María la Real de la Almudena (Madrid). Estos tipos ¿le piedra proceden de canteras localizadas en Viana de Jadraque (Guadalajara) y en el área central de Portugal. Los materiales localizados no corresponden con los tradicionalmente usados en la ciudad de Madrid y su presencia es el resultado de la apertura de nuevas vías de transporte en la segunda mitad del siglo XIX

Nuclear modification of Υ\Upsilon states in pPb collisions at sNN\sqrt{s_\mathrm{NN}} = 5.02 TeV

Production cross sections of $\Upsilon$(1S), $\Upsilon$(2S), and $\Upsilon$(3S) states decaying into \muplusmuminus in proton-lead (pPb) collisions are reported using data collected by the CMS experiment at$\sqrt{s_\mathrm{NN}}$ = 5.02 TeV. A comparison is made with corresponding cross sections obtained with pp data measured at the same collision energy and scaled by the Pb nucleus mass number. The nuclear modification factor for $\Upsilon$(1S) is found to be $R_\mathrm{pPb}(\Upsilon(1S))$ = 0.806 $\pm$ 0.024 (stat) $\pm$ 0.059 (syst). Similar results for the excited states indicate a sequential suppression pattern, such that $R_\mathrm{pPb}(\Upsilon(1S)) \gt R_\mathrm{pPb}(\Upsilon(2S)) \gt R_\mathrm{pPb}(\Upsilon(3S))$. The suppression is much less pronounced in pPb than in PbPb collisions, and independent of transverse momentum $p_\mathrm{T}^\Upsilon$ and center-of-mass rapidity $y_\mathrm{CM}^\Upsilon$ of the individual $\Upsilon$ state in the studied range $p_\mathrm{T}^\Upsilon \lt$ 30 GeV$/c$ and $\vert y_\mathrm{CM}^\Upsilon\vert \lt$ 1.93. Models that incorporate sequential suppression of bottomonia in pPb collisions are in better agreement with the data than those which only assume initial-state modifications