Neuromodulation and neuroprotective effects of chlorogenic acids in excitatory synapses of mouse hippocampal slices

The increased healthspan afforded by coffee intake provides novel opportunities to identify new therapeutic strategies. Caffeine has been proposed to afford benefits through adenosine A2A receptors, which can control synaptic dysfunction underlying some brain disease. However, decaffeinated coffee and other main components of coffee such as chlorogenic acids, also attenuate brain dysfunction, although it is unknown if they control synaptic function. We now used electrophysiological recordings in mouse hippocampal slices to test if realistic concentrations of chlorogenic acids directly affect synaptic transmission and plasticity. 3-(3,4-dihydroxycinnamoyl)quinic acid (CA, 1–10 μM) and 5-O-(trans-3,4-dihydroxycinnamoyl)-D-quinic acid (NCA, 1–10 μM) were devoid of effect on synaptic transmission, paired-pulse facilitation or long-term potentiation (LTP) and long-term depression (LTD) in Schaffer collaterals-CA1 pyramidal synapses. However, CA and NCA increased the recovery of synaptic transmission upon re-oxygenation following 7 min of oxygen/glucose deprivation, an in vitro ischemia model. Also, CA and NCA attenuated the shift of LTD into LTP observed in hippocampal slices from animals with hippocampal-dependent memory deterioration after exposure to β-amyloid 1–42 (2 nmol, icv), in the context of Alzheimer’s disease. These findings show that chlorogenic acids do not directly affect synaptic transmission and plasticity but can indirectly affect other cellular targets to correct synaptic dysfunction. Unraveling the molecular mechanisms of action of chlorogenic acids will allow the design of hitherto unrecognized novel neuroprotective strategies

Fatores associados a hábitos de saúde bucal e utilização de serviços odontológicos entre adolescentes Factors associated with oral health habits and use of dental services by adolescents

Estudo transversal com 1.170 adolescentes da sétima série da rede pública municipal de Gravataí, Rio Grande do Sul, Brasil, investigando a associação de fatores sócio-demográficos, psicossociais e relacionados ao estilo de vida com hábitos de saúde bucal e utilização de serviços odontológicos. Os dados foram analisados por regressão de Cox modificada para estudos transversais. As meninas tiveram maior freqüência de escovação dentária assim como aqueles que relataram não se sentirem discriminados ou sozinhos. O uso diário de fio dental associou-se à melhor inserção sócio-econômica, à busca por serviços privados, à compreensão dos pais e à ausência de sentimento de solidão. A freqüência anual de utilização de serviços odontológicos foi maior entre os de inserção sócio-econômica mais alta. Houve maior busca de serviços por motivo preventivo entre aqueles com melhor inserção sócio-econômica, os que se sentiram compreendidos pelos pais e os que não costumavam consumir doces. Os hábitos de saúde bucal apresentaram associação com inserção sócio-econômica familiar e com fatores psicossociais, exceção à utilização anual de serviços. Quanto ao estilo de vida, o baixo consumo de doces repercutiu positivamente sobre o motivo da visita ao serviço.<br>This was a cross-sectional study of 1,170 seventh-grade adolescents from the municipal public school system in Gravataí, Rio Grande do Sul State, Brazil, investigating the association between socio-demographic, psychosocial, and lifestyle factors and oral health habits and use of dental services. Data were analyzed by Cox regression, modified for cross-sectional studies. Females showed higher frequency of brushing, as did adolescents that reported not feeling alone or discriminated. Daily flossing was associated with higher socioeconomic status (SES), use of private dental services, parental understanding, and lack of feeling of loneliness. Frequency of annual dental visits was higher among individuals with higher SES. Preventive dental checkups were more frequent among individuals with higher SES, those who felt understood by their parents, and those who did not habitually eat candy. Oral health habits were associated with family SES and psychosocial factors except for frequency of annual dental visits. As for lifestyle, low candy consumption had a positive impact on reasons for use of dental appointments

Resumos concluídos - Saúde Coletiva

Resumos concluídos - Saúde Coletiv

Characterisation of microbial attack on archaeological bone

As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones. © 2003 Elsevier Science Ltd. All rights reserved