A model for 3-methyladenine recognition by 3-methyladenine DNA glycosylase I (TAG) from Staphylococcus aureus

The structure of 3-methyladenine DNA glycosylase I in complex with 3-methyladenine is reported

Extensive DNA mimicry by the ArdA anti-restriction protein and its role in the spread of antibiotic resistance

The ardA gene, found in many prokaryotes including important pathogenic species, allows associated mobile genetic elements to evade the ubiquitous Type I DNA restriction systems and thereby assist the spread of resistance genes in bacterial populations. As such, ardA contributes to a major healthcare problem. We have solved the structure of the ArdA protein from the conjugative transposon Tn916 and find that it has a novel extremely elongated curved cylindrical structure with defined helical grooves. The high density of aspartate and glutamate residues on the surface follow a helical pattern and the whole protein mimics a 42-base pair stretch of B-form DNA making ArdA by far the largest DNA mimic known. Each monomer of this dimeric structure comprises three alpha–beta domains, each with a different fold. These domains have the same fold as previously determined proteins possessing entirely different functions. This DNA mimicry explains how ArdA can bind and inhibit the Type I restriction enzymes and we demonstrate that 6 different ardA from pathogenic bacteria can function in Escherichia coli hosting a range of different Type I restriction systems

The protein structure initiative structural genomics knowledgebase

The Protein Structure Initiative Structural Genomics Knowledgebase (PSI SGKB, http://kb.psi-structuralgenomics.org) has been created to turn the products of the PSI structural genomics effort into knowledge that can be used by the biological research community to understand living systems and disease. This resource provides central access to structures in the Protein Data Bank (PDB), along with functional annotations, associated homology models, worldwide protein target tracking information, available protocols and the potential to obtain DNA materials for many of the targets. It also offers the ability to search all of the structural and methodological publications and the innovative technologies that were catalyzed by the PSI's high-throughput research efforts. In collaboration with the Nature Publishing Group, the PSI SGKB provides a research library, editorials about new research advances, news and an events calendar to present a broader view of structural biology and structural genomics. By making these resources freely available, the PSI SGKB serves as a bridge to connect the structural biology and the greater biomedical communitie

Crystallization of Ranasmurfin, a blue-coloured protein from Polypedates leucomystax

A novel blue protein from frog nests has been crystallized

AXIOM: Advanced X-Ray Imaging Of the Magnetosheath

AXIOM (Advanced X-ray Imaging Of the Magnetosphere) is a concept mission which aims to explain how the Earth's magnetosphere responds to the changing impact of the solar wind using a unique method never attempted before; performing wide-field soft X-ray imaging and spectroscopy of the magnetosheath. magnetopause and bow shock at high spatial and temporal resolution. Global imaging of these regions is possible because of the solar wind charge exchange (SWCX) process which produces elevated soft X-ray emission from the interaction of high charge-state solar wind ions with primarily neutral hydrogen in the Earth's exosphere and near-interplanetary space

AXIOM: advanced X-ray imaging of the magnetosphere

Planetary plasma and magnetic field environments can be studied in two complementary ways—by in situ measurements, or by remote sensing. While the former provide precise information about plasma behaviour, instabilities and dynamics on local scales, the latter offers the global view necessary to understand the overall interaction of the magnetospheric plasma with the solar wind. Some parts of the Earth’s magnetosphere have been remotely sensed, but the majority remains unexplored by this type of measurements. Here we propose a novel and more elegant approach employing remote X-ray imaging techniques, which are now possible thanks to the relatively recent discovery of solar wind charge exchange X-ray emissions in the vicinity of the Earth’s magnetosphere. In this article we describe how an appropriately designed and located X-ray telescope, supported by simultaneous in situ measurements of the solar wind, can be used to image the dayside magnetosphere, magnetosheath and bow shock, with a temporal and spatial resolution sufficient to address several key outstanding questions concerning how the solar wind interacts with the Earth’s magnetosphere on a global level. Global images of the dayside magnetospheric boundaries require vantage points well outside the magnetosphere. Our studies have led us to propose ‘AXIOM: Advanced X-ray Imaging of the Magnetosphere’, a concept mission using a Vega launcher with a LISA Pathfinder-type Propulsion Module to place the spacecraft in a Lissajous orbit around the Earth–Moon L1 point. The model payload consists of an X-ray Wide Field Imager, capable of both imaging and spectroscopy, and an in situ plasma and magnetic field measurement package. This package comprises a Proton-Alpha Sensor, designed to measure the bulk properties of the solar wind, an Ion Composition Analyser, to characterise the minor ion populations in the solar wind that cause charge exchange emission, and a Magnetometer, designed to measure the strength and direction of the solar wind magnetic field. We also show simulations that demonstrate how the proposed X-ray telescope design is capable of imaging the predicted emission from the dayside magnetosphere with the sensitivity and cadence required to achieve the science goals of the mission

Exploring Solar-Terrestrial Interactions via Multiple Observers (A White Paper for the Voyage 2050 long-term plan in the ESA Science Programme)

This paper addresses the fundamental science question: "How does solar wind energy flow through the Earth's magnetosphere, how is it converted and distributed?". We need to understand how the Sun creates the heliosphere, and how the planets interact with the solar wind and its magnetic field, not just as a matter of scientific curiosity, but to address a clear and pressing practical problem: space weather, which can influence the performance and reliability of our technological systems, in space and on the ground, and can endanger human life and health. Much knowledge has already been acquired over the past decades, but the infant stage of space weather forecasting demonstrates that we still have a vast amount of learning to do. We can tackle this issue in two ways: 1) By using multiple spacecraft measuring conditions in situ in the magnetosphere in order to make sense of the fundamental small scale processes that enable transport and coupling, or 2) By taking a global approach to observations of the conditions that prevail throughout geospace in order to quantify the global effects of external drivers. A global approach is now being taken by a number of space missions under development and the first tantalising results of their exploration will be available in the next decade. Here we propose the next step-up in the quest for a complete understanding of how the Sun gives rise to and controls the Earth's plasma environment: a tomographic imaging approach comprising two spacecraft which enable global imaging of magnetopause and cusps, auroral regions, plasmasphere and ring current, alongside in situ measurements. Such a mission is going to be crucial on the way to achieve scientific closure on the question of solar-terrestrial interactions

Rats, assorted shit and ‘racist groundwater’: towards extra-sectional understandings of childhoods and social-material processes

Reflecting on a study of children’s outdoor play in a ‘white, working class estate’ in east London, this paper argues that social-material processes that are characteristically massy, indivisible, unseen, fluid and noxious have, problematically, remained hidden-in-plain-sight within multidisciplinary research with children and young people. For example, juxtaposing qualitative and autoethnographic data, we highlight children’s vivid, troubling narratives of swarming rats, smearing excrement, and percolating subsurface flows of water, toxins and racialised affects. In so doing, we develop a wider argument that key theorisations of matter, nature and nonhuman co-presences have often struggled to articulate the indivisibility of social-material processes from contemporary social-political-economic geographies. Over the course of the paper, as children’s raced, classed, exclusionary, disenfranchised narratives accumulate, we recognise the urgency of reconciling microgeographical accounts of play and materiality with readings of geographies of social-economic inequalities, exclusions, ethnicities, religions, memorialisations and mortalities. To this end, we initiate an argument for a move from intersectional to extra-sectional analyses that might retain intersectionality’s critical and political purchase, whilst simultaneously folding social-material complexities and vitalities into its theorisation

Exploring solar-terrestrial interactions via multiple imaging observers

How does solar wind energy flow through the Earth's magnetosphere, how is it converted and distributed? is the question we want to address. We need to understand how geomagnetic storms and substorms start and grow, not just as a matter of scientific curiosity, but to address a clear and pressing practical problem: space weather, which can influence the performance and reliability of our technological systems, in space and on the ground, and can endanger human life and health. Much knowledge has already been acquired over the past decades, particularly by making use of multiple spacecraft measuring conditions in situ, but the infant stage of space weather forecasting demonstrates that we still have a vast amount of learning to do. A novel global approach is now being taken by a number of space imaging missions which are under development and the first tantalising results of their exploration will be available in the next decade. In this White Paper, submitted to ESA in response to the Voyage 2050 Call, we propose the next step in the quest for a complete understanding of how the Sun controls the Earth's plasma environment: a tomographic imaging approach comprising two spacecraft in highly inclined polar orbits, enabling global imaging of magnetopause and cusps in soft X-rays, of auroral regions in FUV, of plasmasphere and ring current in EUV and ENA (Energetic Neutral Atoms), alongside in situ measurements. Such a mission, encompassing the variety of physical processes determining the conditions of geospace, will be crucial on the way to achieving scientific closure on the question of solar-terrestrial interactions.Peer reviewe