Harinosidad en durazno: Diferencias entre cultivares y el proteoma de un mismo cultivar

La oferta de durazno en el mercado está restringida a un período muy corto del año, de octubre a marzo. La presencia en el mercado de cada cultivar, varía de 21 a 35 días. Este período se debe a la amplitud de la fecha de cosecha, 1 a 2 semanas y no más de 3 semanas de almacenaje refrigerado. La principal causa de esa corta vida poscosecha es la manifestación de un desorden fisiológico, asociado a la conservación refrigerada, denominado “harinosidad” cuyo síntoma más característico es la disminución de jugosidad. Durante la temporada 2015/2016 evaluó la frecuencia de aparición de síntomas de harinosidad en los cultivares (cvs) Rich Lady, Ginart, Forastero, Flavorcrest, Gold Prince, Aniversario INTA, Opedepe, Flamer Crest, Red Globe, Spring Lady, Don Carlos INTA, Rojo 2 (de dos lotes diferentes) y Elegant Lady. Se plantearon dos objetivos: 1) conocer la aptitud de los cvs para ser utilizados en programas de mejora genética de la EEA INTA San Pedro y 2) identificar mediante MALDI-TOF/TOF diferencias en el proteoma de frutos harinosos y no harinosos, provenientes de un mismo cultivar, temporada y tratamiento. Los frutos de los 13 cvs se colocaron en cámaras a 0°C durante 21 días y luego de 3 o 4 días a 20°C, cuando alcanzaron la madurez organoléptica, se evaluó el contenido aparente de jugo mediante una escala visual de 0 a 5 (0=Sano y 5 Máxima Harinosidad). Los cultivares Don Carlos INTA, Aniversario INTA y Flavorcrest también se evaluaron luego de 28 días a 0°C y 3 o 4 días a 20°C. Se determinó la media, la moda y los porcentajes de frutos en los diferentes grados de harinosidad en la escala visual. Se encontraron diferencias en la sensibilidad al daño por frío entre cultivares, siendo tolerantes Rich Lady, Forastero, Flavorcrest y Ginart. Destacándose el cv Flavorcrest que alcanzó los 28 días con baja harinosidad. Hasta el momento solo se analizó el proteoma del cultivar Spring Lady. En este cultivar, luego de 21 días a 0°C, se detectaron pocos frutos no harinosos y se identificaron un total de 213 proteínas diferencialmente expresadas entre frutos harinosos y no harinosos. Se encontraron importantes diferencias en los metabolismos proteicos, lipídicos y de pared celular. Además se destaca una marcada aparición de enzimas relacionadas a metabolismos secundarios, como la chalcona-flavona isomerasa y la caroteno desaturasa involucradas en la síntesis de flavonoides y carotenos, respectivamente. En contraste se encontró una notoria desaparición de enzimas encargadas del control del estado redox en los frutos harinosos, como la superóxido dismutasa y una glutatión peroxidasa. Esto podría ser el resultado de una respuesta al estrés por frío generado por el tratamiento o una consecuencia del daño ocasionado.EEA San PedroFil: Monti, Laura L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Lara, María V. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Drincovich, María F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentin

ten year estimated risk of bone fracture in women with differentiated thyroid cancer under tsh suppressive levothyroxine therapy

Introduction: After thyroidectomy and radioiodine therapy, patients with differentiated thyroid cancer (DTC) are indefinitely treated with levothyroxine (L-T4). Osteoporosis is a debated consequence of hypothyroxinaemia. The aim of this study was to evaluate bone mineral density (BMD) and fracture risk assessed by FRAX in a cohort of DTC women. Material and methods: Seventy-four women with DTC (aged 56.5 ± 9.9 years) treated at the mean age of 51.9 ± 12.0 years were studied. Baseline BMD and FRAX were evaluated after 3.0 years (median). BMD and FRAX were further evaluated 5.5 years (median) after the baseline evaluation. A cohort of 120 euthyroid women, matched for age, BMI, and menopausal status, were evaluated as controls. Results: L-T4 dosages were 813.6 ± 208.8 μg/week and 782.1 ± 184.4 μg/week at the baseline and second evaluation, respectively. The risks of major osteoporotic fracture (MOF) and hip fracture (HF) were similar in DTC patients and in controls. In DTC women, significant changes in FRAX were found, with a higher increase in the probability of HF than of MOF. A similar change was found in controls. A significant inverse correlation (P < 0.001) between L-T4 dosage and HF/MOF probability on both first and second evaluations was found. A significant inverse correlation (P = 0.05) was found between fT4, TSH and duration of therapy and HF/MOF probability only on the second evaluation. Conclusions: FRAX increase is a multi-factorial, age-related phenomenon. The absence of correlations between L-T4 dosage, length of therapy or fT4 levels and FRAX does not enable us to attribute an increased fracture risk to DTC women with well-controlled disease on therapy. (Endokrynol Pol 2016; 67 (4): 350–358

Interfering with ROS metabolism in cancer cells: the potential role of quercetin.

Abstract: A main feature of cancer cells, when compared to normal ones, is a persistent pro-oxidative state that leads to an intrinsic oxidative stress. Cancer cells have higher levels of reactive oxygen species (ROS) than normal cells, and ROS are, in turn, responsible for the maintenance of the cancer phenotype. Persistent ROS stress may induce adaptive stress responses, enabling cancer cells to survive with high levels of ROS and maintain cellular viability. However, excessive ROS levels render cancer cells highly susceptible to quercetin, one of the main dietary flavonoids. Quercetin depletes intracellular glutathione and increases intracellular ROS to a level that can cause cell death

Manejo Federal de la Zona Costera Argentina

La Zona Costera Argentina es un continuo fluvio-marítimo de gran diversidad climática y biogeográfica que se extiende desde el extremo norte de la cuenca del Río de la Plata hasta el extremo sur de la costa patagónica. Este trabajo analiza los cambios relacionados con la gestión costera en el período 2009-2019 en políticas públicas, normativa, competencias, responsabilidades, instituciones, instrumentos,formación, capacitación, recursos financieros, conocimiento científico, información y educación ambientales y participación ciudadana. Entre los avances más significativos se encuentran la determinación de los límites de la plataforma continental, la regulación más precisa en el Código Civil y Comercial reformado, la formulación de una Estrategia Federal de Manejo Costero y varias estrategias sectoriales, la creación de la primera área marina protegida, el aumento de los cursos de postgrado relacionados con la gestión costera y la inclusión de contenidos ambientales en todos los niveles del sistema educativo. Por otro lado, cabe destacar la escasez de recursos financieros para el fortalecimiento institucional, la baja incidencia de la participación ciudadana y la persistencia de conflictos de competencia interjurisdiccional. Se concluye que el modelo sectorial de gestión costera se ha consolidado, con una tendencia a migrar de una gestión centralizada a nivel nacional a un sistema federal con mayor capacidad de decisión para las provincias.The Argentine Coastal Zone is a fluvio-maritime continuum of great climatic and biogeographic diversity that extends from the northern end of the Río de la Plata basin to the southern extreme of the Patagonian coast. This paper analyzes the changes related to coastal management in the period 2009-2019 in public policies, regulations, competencies, responsibilities, institutions, instruments, training, capacity building, financial resources, scientific knowledge, environmental information and education, and citizen participation. Among the most significant advances are the determination of the limits of the continental shelf, the more precise regulation in the reformed Civil and Commercial Code, the formulation of a Federal Coastal Management Strategy and several sectoral strategies, the creation of the first marine protected area, the increase of the postgraduate courses related to coastal management, and the expansion of the environmental contents to all levels of the education system. On the other hand, it is worth noting the scarcity of financial resources for institutional strengthening, the low incidence of citizen participation and the persistence of interjurisdictional competition conflicts. It is concluded that the sectoral model of coastal management has consolidated, with a tendency to migrate from a centralized management at the national level to a federal system with greater decision capacity for the provinces.Fil: Dadon, Jose Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad Presidencia; Argentina. Universidad de Buenos Aires. Facultad de Arquitectura, Diseño y Urbanismo. Centro de Investigaciones Gestión de Espacios Costeros; ArgentinaFil: Boscarol, Nadia. Ministerio de Trabajo, Empleo y Seguridad Social; ArgentinaFil: Monti, Alejandro Jorge Arturo. Universidad Nacional de la Patagonia "san Juan Bosco". Facultad de Humanidades y Cs. Sociales (sede Trelew). Instituto de Investigaciones Geograficas de la Patagonia (sede Trelew); ArgentinaFil: García, Mónica Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Humanidades. Departamento de Geografía. Centro de Investigaciones Geográficas Socio-ambientales; ArgentinaFil: Verón, Eleonora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Humanidades. Departamento de Geografía. Centro de Investigaciones Geográficas Socio-ambientales; ArgentinaFil: de Haro, Javier Cristian. Universidad de Buenos Aires. Facultad de Arquitectura, Diseño y Urbanismo. Centro de Investigaciones Gestión de Espacios Costeros; ArgentinaFil: Fevre, Roberto. Universidad de Buenos Aires. Facultad de Arquitectura, Diseño y Urbanismo. Centro de Investigaciones Gestión de Espacios Costeros; ArgentinaFil: Beltrán, Vilma Jacqueline. Universidad Nacional de la Patagonia "san Juan Bosco". Facultad de Humanidades y Cs. Sociales (sede Trelew). Instituto de Investigaciones Geograficas de la Patagonia (sede Trelew); Argentina. Gobierno de la Provincia del Chubut. Ministerio de Ambiente y Control del Desarrollo Sustentable.; ArgentinaFil: Raimondo, Ana María. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Humanidades y Ciencias Sociales Sede Comodoro. Instituto de Investigaciones Geográficas de la Patagonia; ArgentinaFil: Lara, Albina. Universidad de Buenos Aires. Facultad de Ciencias Económicas; ArgentinaFil: Lasta, Carlos Angel. Gobierno de la Provincia de Buenos Aires. Organismo Provincial Para El Desarrollo Sostenible; Argentin

Search for new particles in events with energetic jets and large missing transverse momentum in proton-proton collisions at root s=13 TeV

A search is presented for new particles produced at the LHC in proton-proton collisions at root s = 13 TeV, using events with energetic jets and large missing transverse momentum. The analysis is based on a data sample corresponding to an integrated luminosity of 101 fb(-1), collected in 2017-2018 with the CMS detector. Machine learning techniques are used to define separate categories for events with narrow jets from initial-state radiation and events with large-radius jets consistent with a hadronic decay of a W or Z boson. A statistical combination is made with an earlier search based on a data sample of 36 fb(-1), collected in 2016. No significant excess of events is observed with respect to the standard model background expectation determined from control samples in data. The results are interpreted in terms of limits on the branching fraction of an invisible decay of the Higgs boson, as well as constraints on simplified models of dark matter, on first-generation scalar leptoquarks decaying to quarks and neutrinos, and on models with large extra dimensions. Several of the new limits, specifically for spin-1 dark matter mediators, pseudoscalar mediators, colored mediators, and leptoquarks, are the most restrictive to date.Peer reviewe

Search for invisible decays of the Higgs boson produced via vector boson fusion in proton-proton collisions at s\sqrt{s} = 13 TeV

A search for invisible decays of the Higgs boson produced via vector boson fusion (VBF) has been performed with 101  fb$^{-1}$ of proton-proton collisions delivered by the LHC at $\sqrt{s}$ =13  TeV and collected by the CMS detector in 2017 and 2018. The sensitivity to the VBF production mechanism is enhanced by constructing two analysis categories, one based on missing transverse momentum and a second based on the properties of jets. In addition to control regions with Z and W boson candidate events, a highly populated control region, based on the production of a photon in association with jets, is used to constrain the dominant irreducible background from the invisible decay of a Z boson produced in association with jets. The results of this search are combined with all previous measurements in the VBF topology, based on data collected in 2012 (at $\sqrt{s}$ =8  TeV), 2015, and 2016, corresponding to integrated luminosities of 19.7, 2.3, and 36.3  fb$^{-1}$, respectively. The observed (expected) upper limit on the invisible branching fraction of the Higgs boson is found to be 0.18 (0.10) at the 95% confidence level, assuming the standard model production cross section. The results are also interpreted in the context of Higgs-portal models

Observation of the Bc+_\mathrm{c}^+ Meson in Pb-Pb and pp Collisions at sNN\sqrt{s_{\mathrm{NN}}} = 5.02 TeV and Measurement of its Nuclear Modification Factor

The B$_\mathrm{c}^+$ meson is observed for the first time in heavy ion collisions. Data from the CMS detector are used to study the production of the B$_\mathrm{c}^+$ meson in lead-lead (Pb-Pb) and proton-proton (pp) collisions at a center-of-mass energy per nucleon pair of $\sqrt{s_{\mathrm{NN}}}$ = 5.02 TeV , via the B$_\mathrm{c}^+$ → (J/ψ → μ$^+$μ$^−$)μ$^+$ν$_μ$ decay. The B$_\mathrm{c}^+$ nuclear modification factor, derived from the PbPb-to-pp ratio of production cross sections, is measured in two bins of the trimuon transverse momentum and of the PbPb collision centrality. The B$_\mathrm{c}^+$meson is shown to be less suppressed than quarkonia and most of the open heavy-flavor mesons, suggesting that effects of the hot and dense nuclear matter created in heavy ion collisions contribute to its production. This measurement sets forth a promising new probe of the interplay of suppression and enhancement mechanisms in the production of heavy-flavor mesons in the quark-gluon plasma

Search for top squarks in the four-body decay mode with single lepton final states in proton-proton collisions at s \sqrt{s} = 13 TeV

A search for the pair production of the lightest supersymmetric partner of the top quark, the top squark (t∼1), is presented. The search targets the four-body decay of the t∼1, which is preferred when the mass difference between the top squark and the lightest supersymmetric particle is smaller than the mass of the W boson. This decay mode consists of a bottom quark, two other fermions, and the lightest neutralino (χ∼01), which is assumed to be the lightest supersymmetric particle. The data correspond to an integrated luminosity of 138 fb−1 of proton-proton collisions at a center-of-mass energy of 13 TeV collected by the CMS experiment at the CERN LHC. Events are selected using the presence of a high-momentum jet, an electron or muon with low transverse momentum, and a significant missing transverse momentum. The signal is selected based on a multivariate approach that is optimized for the difference between m(t∼1) and m(χ∼01). The contribution from leading background processes is estimated from data. No significant excess is observed above the expectation from standard model processes. The results of this search exclude top squarks at 95% confidence level for masses up to 480 and 700 GeV for m(t∼1) − m(χ∼01) = 10 and 80 GeV, respectively