Design and fabrication of a novel spinning fluidised bed

Existing vertical spinning fluidised bed (SFB) have several drawbacks, such as non-uniform radial and axial bed fluidisation, feeding and ash accumulation problems. The purpose of this research, therefore is to develop a prototype of the horizontal SFB combustor capable of overcoming these drawbacks. The scopes of the research include engineering design of the prototype, computational fluid dynamics (CFD) modelling and set-up/commissioning of the developed prototype. Under this research, a prototype of the horizontal SFB has been successfully developed and is able to overcome the inherent weakness in vertical SFB. The innovative secondary chamber provides more freeboard for more complete combustion and acts as particulate control device. The prototype is suitable for burning low-density materials (rice husk, fibrous materials), which are difficult to be burnt in conventional fluidised bed by imparting a higher centrifugal force. There is also no limit to the amount of air throughput and combustion is only limited by the kinetics in which each different type of waste burns. Results from the CFD modelling narrowed down the parameters to be tested on the SFB in future experimental works, as well as providing design improvements on the current SFB design. Due to its compactness and versatility in burning a wide range of waste, the SFB prototype has the potential to be utilised as small-scale on-site waste incineration facility and high-efficiency gas burner for high-loading waste gas streams in chemical plants or refineries. The whole system is mountable to a truck and can be transported to waste sources such as rice mills, sawmills, wastewater treatment plants to incinerate waste. The full performance on the developed SFB during combustion of various types of wastes is outside the scope of the current research and therefore, is subjected to future experimental works

TCP HACK: TCP Header Checksum Option to Improve Performance Over Lossy Links

In recent years, wireless networks have become increasingly common and an increasing number of devices are communicating with each other over lossy links. Unfortunately, TCP performs poorly over lossy links as it is unable to differentiate the loss due to packet corruption from that due to congestion. In this paper, we present an extension to TCP which enables TCP to distinguish packet corruption from congestion in lossy environments resulting in improved performance. We refer to this extension as the HeAder ChecKsum option (HACK). We implemented our algorithm in the Linux kernel and performed various tests to determine its effectiveness. Our results have shown that HACK performs substantially better than both SACK and NewReno in cases where burst corruptions are frequent. We also found that HACK can co-exist very nicely with SACK and performs even better with SACK enabled

Loss of viability during freeze-thaw of intact and adherent human embryonic stem cells with conventional slow-cooling protocols is predominantly due to apoptosis rather than cellular necrosis

10.1007/s11373-005-9051-9Journal of Biomedical Science133433-44

Entomologic and Virologic Investigation of Chikungunya, Singapore

Data from longitudinal analyses can be useful in the design and implementation of control strategies

A Comparison of Assays for Accurate Copy Number Measurement of the Low-Affinity Fc Gamma Receptor Genes FCGR3A and FCGR3B

The FCGR3 locus encoding the low affinity activating receptor FcγRIII, plays a vital role in immunity triggered by cellular effector and regulatory functions. Copy number of the genes FCGR3A and FCGR3B has previously been reported to affect susceptibility to several autoimmune diseases and chronic inflammatory conditions. However, such genetic association studies often yield inconsistent results; hence require assays that are robust with low error rate. We investigated the accuracy and efficiency in estimating FCGR3 CNV by comparing Sequenom MassARRAY and paralogue ratio test-restriction enzyme digest variant ratio (RT-REDVR). In addition, since many genetic association studies of FCGR3B CNV were carried out using real-time quantitative PCR, we have also included the evaluation of that method’s performance in estimating the multi-allelic CNV of FCGR3B. The qPCR assay exhibited a considerably broader distribution of signal intensity, potentially introducing error in estimation of copy number and higher false positive rates. Both Sequenom and PRT-REDVR showed lesser systematic bias, but Sequenom skewed towards copy number normal (CN = 2). The discrepancy between Sequenom and PRT-REDVR might be attributed either to batch effects noise in individual measurements. Our study suggests that PRT-REDVR is more robust and accurate in genotyping the CNV of FCGR3, but highlights the needs of multiple independent assays for extensive validation when performing a genetic association study with multi-allelic CNVs

Cardiovascular disease-related miRNAs expression: potential role as biomarkers and effects of training exercise

Cardiovascular diseases (CVDs) are one of the most important causes of mortality worldwide, therefore the need of effective preventive strategies is imperative. Aging is associated with significant changes in both cardiovascular structure and function that lower the threshold for clinical signs and symptoms, making older people more susceptible to CVDs morbidity and mortality. microRNAs (miRNAs) modulate gene expression at post-transcriptional level and increasing evidence has shown that miRNAs are involved in cardiovascular physiology and in the pathogenesis of CVDs. Physical activity is recommended by the medical community and the cardiovascular benefits of exercise are multifactorial and include important systemic effects on skeletal muscle, the peripheral vasculature, metabolism, and neuroendocrine systems, as well as beneficial modifications within the myocardium itself. In this review we describe the role of miRNAs and their dysregulation in several types of CVDs. We provide an overview of miRNAs in CVDs and of the effects of physical activity on miRNA regulation involved in both cardiovascular pathologies and age-related cardiovascular changes and diseases. Circulating miRNAs in response to acute and chronic sport exercise appear to be modulated following training exercise, and may furthermore serve as potential biomarkers for CVDs and different age-related CVDs

Identification of Close Relatives in the HUGO Pan-Asian SNP Database

The HUGO Pan-Asian SNP Consortium has recently released a genome-wide dataset, which consists of 1,719 DNA samples collected from 71 Asian populations. For studies of human population genetics such as genetic structure and migration history, this provided the most comprehensive large-scale survey of genetic variation to date in East and Southeast Asia. However, although considered in the analysis, close relatives were not clearly reported in the original paper. Here we performed a systematic analysis of genetic relationships among individuals from the Pan-Asian SNP (PASNP) database and identified 3 pairs of monozygotic twins or duplicate samples, 100 pairs of first-degree and 161 second-degree of relationships. Three standardized subsets with different levels of unrelated individuals were suggested here for future applications of the samples in most types of population-genetics studies (denoted by PASNP1716, PASNP1640 and PASNP1583 respectively) based on the relationships inferred in this study. In addition, we provided gender information for PASNP samples, which were not included in the original dataset, based on analysis of X chromosome data

Population Genetic Structure of Peninsular Malaysia Malay Sub-Ethnic Groups

Patterns of modern human population structure are helpful in understanding the history of human migration and admixture. We conducted a study on genetic structure of the Malay population in Malaysia, using 54,794 genome-wide single nucleotide polymorphism genotype data generated in four Malay sub-ethnic groups in peninsular Malaysia (Melayu Kelantan, Melayu Minang, Melayu Jawa and Melayu Bugis). To the best of our knowledge this is the first study conducted on these four Malay sub-ethnic groups and the analysis of genotype data of these four groups were compiled together with 11 other populations' genotype data from Indonesia, China, India, Africa and indigenous populations in Peninsular Malaysia obtained from the Pan-Asian SNP database. The phylogeny of populations showed that all of the four Malay sub-ethnic groups are separated into at least three different clusters. The Melayu Jawa, Melayu Bugis and Melayu Minang have a very close genetic relationship with Indonesian populations indicating a common ancestral history, while the Melayu Kelantan formed a distinct group on the tree indicating that they are genetically different from the other Malay sub-ethnic groups. We have detected genetic structuring among the Malay populations and this could possibly be accounted for by their different historical origins. Our results provide information of the genetic differentiation between these populations and a valuable insight into the origins of the Malay sub-ethnic groups in Peninsular Malaysia