Detection of He I λ10830\lambda10830 \AA{} absorption on HD 189733 b with CARMENES high-resolution transmission spectroscopy

We present three transit observations of HD 189733 b obtained with the high-resolution spectrograph CARMENES at Calar Alto. A strong absorption signal is detected in the near-infrared He I triplet at 10830 \AA{} in all three transits. During mid-transit, the mean absorption level is $0.88\pm0.04$ % measured in a $\pm$10 km s$^{-1}$ range at a net blueshift of $-3.5\pm0.4$ km s$^{-1}$ (10829.84--10830.57 \AA{}). The absorption signal exhibits radial velocities of $+6.5\pm3.1$ km s$^{-1}$ and $-12.6\pm1.0$ km s$^{-1}$ during ingress and egress, respectively; measured in the planetary rest frame. We show that stellar activity related pseudo-signals interfere with the planetary atmospheric absorption signal. They could contribute as much as 80% of the observed signal and might also affect the radial velocity signature, but pseudo-signals are very unlikely to explain the entire signal. The observed line ratio between the two unresolved and the third line of the He I triplet is $2.8\pm0.2$, which strongly deviates from the value expected for an optically thin atmospheres. When interpreted in terms of absorption in the planetary atmosphere, this favors a compact helium atmosphere with an extent of only 0.2 planetary radii and a substantial column density on the order of $4\times 10^{12}$ cm$^{-2}$. The observed radial velocities can be understood either in terms of atmospheric circulation with equatorial superrotation or as a sign of an asymmetric atmospheric component of evaporating material. We detect no clear signature of ongoing evaporation, like pre- or post-transit absorption, which could indicate material beyond the planetary Roche lobe, or radial velocities in excess of the escape velocity. These findings do not contradict planetary evaporation, but only show that the detected helium absorption in HD 189733 b does not trace the atmospheric layers that show pronounced escape signatures.Comment: 13 pages, 12 figures, accepted for publication in A&

Design of an innovative learning experience for the final project of the building engineering degree

This essay presents the bases for a new teaching methodology for the Final Project of the Degree of Building Engineering. The aim of this methodology is to approach students to advanced forms of work in architectural and engineering offices by employing Building Information Modelling (BIM) technologies. This initiative has been funded within the Call 2011 for Teaching Research Incentives ofthe I Teaching Plan of the University of Seville. Following the guidelines of the European Higher Education Area, the learning experience designed has to enable the future Building Engineers to acquire specific and generic competences ascribed to the Final Project in the Verification Report of the Building Engineering Degree. The specific competence “E71. Presentation and defence before a university board of a final project, consisting of an integration exercise of the formative contents received and skills acquired through t he degree” is trained by the development of a building execution project with the use of BIM technologies. For a decade, architecture and engineering offices have increasingly been incorporating in their projects new tools for information processing in digital integrated systems, i.e. programs which allow the construction of building virtual models in three dimensions, and the identification of their constructive components, providing them with parametric dimensions. The operating capacity of BIM programs is stronger than that of 2D drawing programs, since they can manage and generate all the technical documentation in an integrated way. As far as the generic competences are concerned, the problem is that the Final Project has ascribed twenty four competences and their training and evaluation throughout a single term, which seems rather unattainable. In order to solve this matter, the four most important generic competences of Building Engineers have been identified according to their professional profile: “G01. Capacity for organization and planning”, “G06. Information management skills”, “G09. Ability to work in an interdisciplinary team” and “G13. Positive social attitude towards social and technological innovations”. The use of BIM technologies and collaborative work methodologies allow the training of these genericcompetences. Finally, assessment matrixes of the five competences involved have been established with the descriptors of the assessment indicators for each of their corresponding criteria at each level of student achievement. This study is limited to the design of the experience; its implementation could be carried out in the first term of the 2012/2013 academic year, provided the main pre-requisite are met by students, and command of BIM programs such as ALLPLAN, REVIT or ARCHICAD, is achieved. Aware of this challenge− since BIM programs are taught as optional subjects− a curricular line for students interested in participating in this experience is also proposed for the next academic yea

Sex-related differences of fatty acid-binding protein 4 and leptin levels in atrial fibrillation

Aims: Adiposity plays a key role in the pathogenesis of atrial fibrillation (AF). Our aim was to study the sex differences in adipokines levels according to AF burden. Methods and results: Two independent cohorts of patients were studied: (i) consecutive patients with AF undergoing catheter ablation (n = 217) and (ii) a control group (n = 105). (i) Adipokines, oxidative stress, indirect autonomic markers, and leucocytes mRNA levels were analysed; (ii) correlation between biomarkers was explored with heatmaps and Kendall correlation coefficients; and (iii) logistic regression and random forest model were used to determine predictors of AF recurrence after ablation. Our results showed that: (i) fatty acid-binding protein 4 (FABP4) and leptin levels were higher in women than in men in both cohorts (P < 0.01). In women, FABP4 levels were higher on AF cohort (20 ± 14 control, 29 ± 18 paroxysmal AF and 31 ± 17 ng/mL persistent AF; P < 0.01). In men, leptin levels were lower on AF cohort (22 ± 15 control, 13 ± 16 paroxysmal AF and 13 ± 11 ng/mL persistent AF; P < 0.01). (ii) In female with paroxysmal AF, there was a lower acetylcholinesterase and higher carbonic anhydrase levels with respect to men (P < 0.05). (iii) Adipokines have an important role on discriminate AF recurrence after ablation. In persistent AF, FABP4 was the best predictor of recurrence after ablation (1.067, 95% confidence interval 1-1.14; P = 0.046). Conclusion: The major finding of the present study is the sex-based differences of FABP4 and leptin levels according to AF burden. These adipokines are associated with oxidative stress, inflammatory and autonomic indirect markers, indicating that they may play a role in AF perpetuation.This study was supported by projects (PI16/01282 and PI18/01584) integrated in the Plan Estatal de I+D+I 2016–2019 and cofounded by ISCIII-Subdirección General de Evaluación y Fomento de la Investigación del Fondo Europeo de Desarrollo Regional (FEDER). J.N.L.-C. and M.R.-M. were a recipient of a Sociedade Galega de Cardioloxía (SOGACAR) research grant. D.d.G.-C. was a recipient of a Juan de la Cierva-Incorporación grant from the Ministry of Science Innovation and Universities (IJCI-2016-29393). CIBER Cardiovascular (CB16/11/00403 to V.Ll.-C. and D.d.G.-C.) is a project from Carlos III Health Institute.Peer reviewe

Magnetic Fields in Massive Star-forming Regions (MagMaR). I. Linear Polarized Imaging of the Ultracompact H II Region G5.89-0.39

We report 1.2 mm polarized continuum emission observations carried out with the Atacama Large Millimeter/submillimeter Array toward the high-mass star formation region G5.89-0.39. The observations show a prominent 0.2 pc north-south filamentary structure. The ultracompact H ii region in G5.89-0.39 breaks the filament into two pieces. Its millimeter emission shows a dusty belt with a mass of 55-115 M o˙ and 4500 au in radius, surrounding an inner part comprising mostly ionized gas, with dust emission only accounting for about 30% of the total millimeter emission. We also found a lattice of convex arches that may be produced by dragged dust and gas from the explosive dispersal event involving the O5 Feldt's star. The north-south filament has a mass between 300 and 600 M o˙ and harbors a cluster of about 20 mm envelopes with a median size and mass of 1700 au and 1.5 M o˙, respectively, some of which are already forming protostars. We interpret the polarized emission in the filament as mainly coming from magnetically aligned dust grains. The polarization fraction is ∼4.4% in the filaments and 2.1% at the shell. The magnetic fields are along the North Filament and perpendicular to the South Filament. In the Central Shell, the magnetic fields are roughly radial in a ring surrounding the dusty belt between 4500 and 7500 au, similar to the pattern recently found in the surroundings of Orion BN/KL. This may be an independent observational signpost of explosive dispersal outflows and should be further investigated in other regions.P.S. was partially supported by a Grant-in-Aid for Scientific Research (KAKENHI No. 18H01259) of the Japan Society for the Promotion of Science (JSPS). L.A.Z. acknowledges financial support from CONACyT-280775 and UNAM-PAPIIT IN110618 grants, México. C.L.H.H. acknowledges the support of the NAOJ Fellowship and JSPS KAKENHI grants 18K13586 and 20K14527. J.M.G. is supported by the grant AYA2017-84390-C2-R (AEI/FEDER, EU). K.T. was supported by JSPS KAKENHI grant No. 20H05645. The National Radio Astronomy Observatory is a facility of the National Science Foundation operated under cooperative agreement by Associated Universities, Inc

A multivariable prognostic score to guide systemic therapy in early-stage HER2-positive breast cancer: a retrospective study with an external evaluation

Background: In early-stage HER2-positive breast cancer, escalation or de-escalation of systemic therapy is a controversial topic. As an aid to treatment decisions, we aimed to develop a prognostic assay that integrates multiple data types for predicting survival outcome in patients with newly diagnosed HER2-positive breast cancer. Methods: We derived a combined prognostic model using retrospective clinical–pathological data on stromal tumour-infiltrating lymphocytes, PAM50 subtypes, and expression of 55 genes obtained from patients who participated in the Short-HER phase 3 trial. The trial enrolled patients with newly diagnosed, node-positive, HER2-positive breast cancer or, if node negative, with at least one risk factor (ie, tumour size >2 cm, histological grade 3, lymphovascular invasion, Ki67 >20%, age ≤35 years, or hormone receptor negativity), and randomly assigned them to adjuvant anthracycline plus taxane-based combinations with either 9 weeks or 1 year of trastuzumab. Trastuzumab was administered intravenously every 3 weeks (8 mg/kg loading dose at first cycle, and 6 mg/kg thereafter) for 18 doses or weekly (4 mg/kg loading dose in the first week, and 2 mg/kg thereafter) for 9 weeks, starting concomitantly with the first taxane dose. Median follow-up was 91·4 months (IQR 75·1–105·6). The primary objective of our study was to derive and evaluate a combined prognostic score associated with distant metastasis-free survival (the time between randomisation and distant recurrence or death before recurrence), an exploratory endpoint in Short-HER. Patient samples in the training dataset were split into a training set (n=290) and a testing set (n=145), balancing for event and treatment group. The training set was further stratified into 100 iterations of Monte-Carlo cross validation (MCCV). Cox proportional hazard models were fit to MCCV training samples using Elastic-Net. A maximum of 92 features were assessed. The final prognostic model was evaluated in an independent combined dataset of 267 patients with early-stage HER2-positive breast cancer treated with different neoadjuvant and adjuvant anti-HER2-based combinations and from four other studies (PAMELA, CHER-LOB, Hospital Clinic, and Padova) with disease-free survival outcome data. Findings: From Short-HER, data from 435 (35%) of 1254 patients for tumour size (T1 vs rest), nodal status (N0 vs rest), number of tumour-infiltrating lymphocytes (continuous variable), subtype (HER2-enriched and basal-like vs rest), and 13 genes composed the final model (named HER2DX). HER2DX was significantly associated with distant metastasis-free survival as a continuous variable (p<0·0001). HER2DX median score for quartiles 1–2 was identified as the cutoff to identify low-risk patients; and the score that distinguished quartile 3 from quartile 4 was the cutoff to distinguish medium-risk and high-risk populations. The 5-year distant metastasis-free survival of the low-risk, medium-risk, and high-risk populations were 98·1% (95% CI 96·3–99·9), 88·9% (83·2–95·0), and 73·9% (66·0–82·7), respectively (low-risk vs high-risk hazard ratio [HR] 0·04, 95% CI 0·0–0·1, p<0·0001). In the evaluation cohort, HER2DX was significantly associated with disease-free survival as a continuous variable (HR 2·77, 95% CI 1·4–5·6, p=0·0040) and as group categories (low-risk vs high-risk HR 0·27, 0·1–0·7, p=0·005). 5-year disease-free survival in the HER2DX low-risk group was 93·5% (89·0–98·3%) and in the high-risk group was 81·1% (71·5–92·1). Interpretation: The HER2DX combined prognostic score identifies patients with early-stage, HER2-positive breast cancer who might be candidates for escalated or de-escalated systemic treatment. Future clinical validation of HER2DX seems warranted to establish its use in different scenarios, especially in the neoadjuvant setting. Funding: Instituto Salud Carlos III, Save the Mama, Pas a Pas, Fundación Científica, Asociación Española Contra el Cáncer, Fundación SEOM, National Institutes of Health, Agenzia Italiana del Farmaco, International Agency for Research on Cancer, and the Veneto Institute of Oncology, and Italian Association for Cancer Research

Multicentric standardization of protocols for the diagnosis of human mitochondrial respiratory chain defects

The quantification of mitochondrial respiratory chain (MRC) enzymatic activities is essential for diagnosis of a wide range of mitochondrial diseases, ranging from inherited defects to secondary dysfunctions. MRC lesion is frequently linked to extended cell damage through the generation of proton leak or oxidative stress, threatening organ viability and patient health. However, the intrinsic challenge of a methodological setup and the high variability in measuring MRC enzymatic activities represents a major obstacle for comparative analysis amongst institutions. To improve experimental and statistical robustness, seven Spanish centers with extensive experience in mitochondrial research and diagnosis joined to standardize common protocols for spectrophotometric MRC enzymatic measurements using minimum amounts of sample. Herein, we present the detailed protocols, reference ranges, tips and troubleshooting methods for experimental and analytical setups in different sample preparations and tissues that will allow an international standardization of common protocols for the diagnosis of MRC defects. Methodological standardization is a crucial step to obtain comparable reference ranges and international standards for laboratory assays to set the path for further diagnosis and research in the field of mitochondrial diseases. © 2022 by the authors. Licensee MDPI, Basel, Switzerland

Спостереження як метод державного фінансового контролю за сплатою податків суб'єктами малого підприємництва

У статті проаналізовано особливості державного контролю за діяльністю суб’єктів малого підприємництва. Наведено авторське визначення методу спостереження в державному фінансовому контролі за сплатою податків та обґрунтовано доцільність його застосування в перспективі. (The features of state control over the activities of small businesses are analyzed. The author’s definition of the method of monitoring in the state financial control over tax payments is given, and the expedience for its use in the prospects is grounded.

Green electrochemical template synthesis of CoPt nanoparticles with tunable size, composition, and magnetism from microemulsions using an ionic liquid (bmimPF6)

Altres ajuts: Substrates have been prepared in IMB-CNM (CSIC),supported by the (CSIC) NGG-258 project.Electrodeposition from microemulsions using ionic liquids is revealed as a green method for synthesizing magnetic alloyed nanoparticles, avoiding the use of aggressive reducing agents. Microemulsions containing droplets of aqueous solution (electrolytic solution containing Pt(IV) and Co(II) ions) in an ionic liquid (bmimPF) define nanoreactors in which the electrochemical reduction takes place. Highly crystalline hcp alloyed CoPt nanoparticles, in the 10-120 nm range with a rather narrow size distribution, have been deposited on a conductive substrate. The relative amount of aqueous solution to ionic liquid determines the size of the nanoreactors, which serve as nanotemplates for the growth of the nanoparticles and hence determine their size and distribution. Further, the stoichiometry (PtCo) of the particles can be tuned by the composition of the electrolytic solution inside the droplets. The control of the size and composition of the particles allows tailoring the room-temperature magnetic behavior of the nanoparticles from superparaparamagnetic to hard magnetic (with a coercivity of H = 4100 Oe) in the as-obtained state. © 2014 American Chemical Society

Earliest Known Use of Marine Resources by Neanderthals

Numerous studies along the northern Mediterranean borderland have documented the use of shellfish by Neanderthals but none of these finds are prior to Marine Isotopic Stage 3 (MIS 3). In this paper we present evidence that gathering and consumption of mollusks can now be traced back to the lowest level of the archaeological sequence at Bajondillo Cave (Málaga, Spain), dated during the MIS 6. The paper describes the taxonomical and taphonomical features of the mollusk assemblages from this level Bj19 and briefly touches upon those retrieved in levels Bj18 (MIS 5) and Bj17 (MIS 4), evidencing a continuity of the shellfishing activity that reaches to MIS 3. This evidence is substantiated on 29 datings through radiocarbon, thermoluminescence and U series methods. Obtained dates and paleoenvironmental records from the cave include isotopic, pollen, lithostratigraphic and sedimentological analyses and they are fully coherent with paleoclimate conditions expected for the different stages. We conclude that described use of shellfish resources by Neanderthals (H. neanderthalensis) in Southern Spain started ∼150 ka and were almost contemporaneous to Pinnacle Point (South Africa), when shellfishing is first documented in archaic modern humans