Simulation of a biorefinery process as learning tool in chemical engineering degree

Currently, the search for alternative biomass to be used as renewable sources for energy production is one of the most important challenges to achieve a sustainable growth based on a bioeconomy strategy (Mendes et al., 2009). In this context, lignocellulosic waste are a renewable, clean, inexpensive and with high availability for the manufacture of biofuels. In this sense, the main objective of this study was the simulation and design of engineering processes that allows the valorization of lignocellulosic waste and the obtaining of biofuels as alternative to fossil fuels. This goal implies the practical application of the theoretical knowledge acquired by the student during the chemical engineering degree. Specifically, the students designed and simulated a biorefinery process that consisted of a Kraft pulping process as starting point of two main lines of production: (1) Process I: production of bioethanol and (2) Process II: direct and indirect production of dimethylether (DME), both from lignocellulosic biomass (Fig.1). Two commercial simulation packages, ASPEN HYSYS® and UNISIM were used to simulate the production of dimethylether and bioethanol, respectively. The first step was determining a strategic situation for the installation of the biorefinery. The central area of Andalusia (between the municipalities of Lucena and Antequera) was considered the most adequate area to develop the installation of the biorefinery plant after evaluating the biomass available inside of 100 kms of distance around this place. Specifically, different biomass mixtures were considered in order to ensure the viability of a constant inlet flow of biomass in the biorefinery. In function of this inlet flow of biomass, the installations were designed and dimensioned in each stage of the process. The student carried out a wide revision of state of the art to decide the most adequate processes among different alternatives to obtain dimethylether and bioethanol. The different stages selected as the most adequate in each line of the process can be observed in Fig.1. Moreover, the students evaluated the different alternatives for the valorisation and optimization of the by-products generated in each stage of the process in order to minimize the consuming of chemical compounds and energy requirements. Therefore, the students learnt to develop a real engineering process more sustainable and friendly with the environment. To sum up, the used of programs to simulate the transformation of lignocellulosic biomass in biofuels, such as, bioethanol or dimethylether, which is a process with several social, environmental and economic advantages, was an interesting learning tool for students of chemical engineering degree. Keywords Bioethanol, design, dimethylether, Kraft pulping process, simulations. References Mendes, C.V.T., Carvalho, M.G.V.S., Baptista, C.M.S.G., Rocha, J.M.S., Soares, B.I.G., Sousa, G.D.A., 2009. Valorisation of hardwood hemicelluloses in the kraft pulping process by using an integrated biorefinery concept. Food Bioproduct Process 87:197–207.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech MINECO: Proyecto CTQ2015-68654-

Transporte marítimo de corta distancia entre España e Italia: perfil de las empresas usuarias

El Transporte Marítimo de Corta Distancia (TMCD) ha sido catalogado por la Comisión Europea como el único modo de transporte que ofrece posibilidades reales de lograr un transvase de carga desde la carretera, mejorando además la competitividad y reduciendo los costes ambientales. En los últimos años se han llevado a cabo diversos estudios donde se han tratado de identificar líneas de TMCD con un potencial de futuro importante. Sin embargo, no se han abordado trabajos donde se evalúe el perfil de las empresas de transporte internacional por carretera que hacen uso de esta modalidad de transporte. Con el propósito de cubrir este déficit, en el presente trabajo se procede, sobre una muestra de 81 empresas de transporte internacional por carretera (41 españolas, 3 portuguesas y 37 italianas) que hacen uso de las líneas de TMCD entre España e Italia, a delimitar el perfil de dichas empresas, a la vez que a analizar ciertos elementos inherentes a la relación que mantienen con las navieras con las que trabaja

Learning and Assessing Competencies: New challenges for Mathematics in Engineering Degrees in Spain.

The introduction of new degrees adapted to the European Area of Higher Education (EAHE) has involved a radically different approach to the curriculum. The new programs are structured around competencies that should be acquired. Considering the competencies, teachers must define and develop learning objectives, design teaching methods and establish appropriate evaluation systems. While most Spanish universities have incorporated methodological innovations and evaluation systems different from traditional exams, there is enough confusion about how to teach and assess competencies and learning outcomes, as traditionally the teaching and assessment have focused on knowledge. In this paper we analyze the state-of-the-art in the mathematical courses of the new engineering degrees in some Spanish universities

OPTIMIZACIÓN DE GESTIÓN DE ALMACÉN Y COMPRAS DE LA EMPRESA “MATIZADOS Y DECORACIONES” MEDIANTE RECONOCIMIENTO DE PATRONES POR PREDICCIÓN DE SERIES DE TIEMPO

RECONOCIMIENTO DE PATRONES SERIES DE TIEMPO RED NEURONAL VISUAL STUDIO VISUAL C# MYSQL NET FRAMEWORK 4.0 FUNCIÓN SIGMOIDEA ARQUITECTURA VON NEUMANN ESTADO DEL ARTE PREDICCIÓN EN SERIES DE TIEMPO CON MODELOS ADITIVOS RECONOCIMIENTO DE LENGUAJES DE SIGNOS MEDIANTE REDES NEURONALES PREDICCIÓN CON REDES NEURONALES: COMPARACIÓN CON LAS METODOLOGÍAS DE BOX Y JENKIN

The Made In Effect on the Perception of a Spanish Industrial Product in Foreign Markets

With globalization, organizations must be able to look for their own differentiation with the aim to obtain sustainable competitive advantages. Differentiation across the products can be a key factor if it is also associated with their place of origin. More and more, if we consider that the nowadays economical crisis is affecting several of the most representative sectors and industries. In this respect, this paper has as aim to analyze the perception that professionals of the construction industry of some of the main European markets, exactly, France, Poland and United Kingdom, have on different types of ceramic tiles having into account their place of origin.Ante la globalización, las empresas deben ser capaces de buscar sus propios parámetros de diferenciación con el fin de obtener ventajas competitivas sostenibles. La diferenciación a través de los productos puede ser un factor clave si además va asociada a su lugar de origen. Además la crisis de las principales economías mundiales afecta a varios de sus sectores más representativos. El presente trabajo tiene como objetivo analizar la percepción que profesionales del sector de la construcción de algunos de los principales mercados europeos, concretamente, Francia, Polonia y Reino Unido, tienen sobre diferentes tipos de recubrimiento para suelos en función de su lugar de origen

CARGO: a web portal to integrate customized biological information

There is a huge quantity of information generated in Life Sciences, and it is dispersed in many databases and repositories. Despite the broad availability of the information, there is a great demand for methods that are able to look for, gather and display distributed data in a standardized and friendly way. CARGO (Cancer And Related Genes Online) is a configurable biological web portal designed as a tool to facilitate, integrate and visualize results from Internet resources, independently of their native format or access method. Through the use of small agents, called widgets, supported by a Rich Internet Application (RIA) paradigm based on AJAX, CARGO provides pieces of minimal, relevant and descriptive biological information. The tool is designed to be used by experimental biologists with no training in bioinformatics. In the current state, the system presents a list of human cancer genes. Available at http://cargo.bioinfo.cnio.e

Teknik Tekstil Endüstrileri: Nonwoven Kumaş Örneği Ve Gaziantep’in Durumu

"Teknik tekstiller" kavramı ilk ortaya çıktığında sadece endüstriyel amaçlı (giysi ve ev tekstili dışında) tekstil malzemeleri için kullanılan bir ifade iken şu an geniş bir sektörü temsil eden bir kavrama dönüşmüştür. 130 milyar $'lık bir piyasa ve yüksek bir katma değer ile birçok ülke ve yatırımcının tercih ettiği bir sektör haline gelmiştir. Yıllar geçtikçe, teknolojinin de gelişimiyle, teknik tekstil üretiminde ve uygulama alanlarında büyük çapta artma olmuş; çok farklı özelliklere sahip lifler üretilmeye başlanmıştır. Teknik tekstiller, önceleri askeri ve sportif giysilerde kullanılmış; sonraları moda tasarımcılarının bu malzemeleri koleksiyonlarına taşımalarıyla, yüksek teknolojik tekstiller, günlük giysilere de adapte edilmeye başlanmıştır. 90'lı yıllardan sonra elektronik ürünlerin giysilerimize entegre edilmesiyle birlikte teknik tekstil üretimi yeni bir boyut kazanmıştır. Endüstriyel tekstil malzemesi üreticileri, moda tasarımcıları, tekstil sanatçıları ve bilim adamlarına kadar alanında uzman birçok kişinin katılımıyla teknik/akıllı malzemeler alanında yeni ürünler ortaya konmaktadır. Bu çalışmada, teknik tekstil piyasasının genişliği ve üretim açısından önde yer alan ülkelerdeki durumunun ne aşamada olduğu, gelecekte piyasanın ulaşacağı seviye ve ülkemizdeki durum hakkında veriler bir araya getirilerek sektörün genel bir bakış açısı ile değerlendirme imkânı sunmaya çalışılmıştır. İlk olarak teknik tekstil kavramları açıklanmış, daha sonra teknik tekstil sınıflamaları üzerinde durulmuştur. Dünya genelinde trend bir sektör haline gelen bu alanda genel durum ve ülkemizin durumu ortaya konmaya çalışılmış, iktisadi açıdan piyasa yapısı analiz edilmeye çalışılmış, yoğunlaşma ve Herfindal ve Hirschman endeksleri uygulanarak sektör yapısı hakkında daha net veriler ortaya konmuştur. Gaziantep'teki firmalar üzerinde yapılan bu endüstri yapısı değerlendirmesinden sonra firmaların mevcut durumlarını değerlendirmek için Swot analizi yapılmıştır

The role of clonal communication and heterogeneity in breast cancer

Background: Cancer is a rapidly evolving, multifactorial disease that accumulates numerous genetic and epigenetic alterations. This results in molecular and phenotypic heterogeneity within the tumor, the complexity of which is further amplified through specific interactions between cancer cells. We aimed to dissect the molecular mechanisms underlying the cooperation between different clones. Methods: We produced clonal cell lines derived from the MDA-MB-231 breast cancer cell line, using the UbC-StarTrack system, which allowed tracking of multiple clones by color: GFP C3, mKO E10 and Sapphire D7. Characterization of these clones was performed by growth rate, cell metabolic activity, wound healing, invasion assays and genetic and epigenetic arrays. Tumorigenicity was tested by orthotopic and intravenous injections. Clonal cooperation was evaluated by medium complementation, co-culture and co-injection assays. Results: Characterization of these clones in vitro revealed clear genetic and epigenetic differences that affected growth rate, cell metabolic activity, morphology and cytokine expression among cell lines. In vivo, all clonal cell lines were able to form tumors; however, injection of an equal mix of the different clones led to tumors with very few mKO E10 cells. Additionally, the mKO E10 clonal cell line showed a significant inability to form lung metastases. These results confirm that even in stable cell lines heterogeneity is present. In vitro, the complementation of growth medium with medium or exosomes from parental or clonal cell lines increased the growth rate of the other clones. Complementation assays, co-growth and co-injection of mKO E10 and GFP C3 clonal cell lines increased the efficiency of invasion and migration. Conclusions: These findings support a model where interplay between clones confers aggressiveness, and which may allow identification of the factors involved in cellular communication that could play a role in clonal cooperation and thus represent new targets for preventing tumor progression

Ig-Like Transcript 2 (ILT2) Blockade and Lenalidomide Restore NK Cell Function in Chronic Lymphocytic Leukemia

One of the cardinal features of chronic lymphocytic leukemia (CLL) is its association with a profound immunosuppression. NK cell function is markedly impaired in CLL patients, who show a significant dysregulation of the expression of activating and inhibitory receptors. Here, we analyzed the role of the novel inhibitory receptor Ig-like transcript 2 (ILT2, also termed LIR-1, LILRB1) in the regulation of NK cells in CLL. Our results show that ILT2 expression was significantly decreased on leukemic cells and increased on NK cells of CLL patients, particularly in those with advanced disease and with bad prognostic features, such as those carrying chromosome del(11q). The immunomodulatory drug lenalidomide may regulate the expression of ILT2 and its ligands in CLL since it significantly increased the expression of ILT2 and partially reestablished the expression of its ligands on leukemic cells. Furthermore, lenalidomide significantly increased the activation and proliferation of NK cells, which was strongly enhanced by ILT2 blockade. Combining ILT2 blockade and lenalidomide activated NK cell cytotoxicity resulting in increased elimination of leukemic cells from CLL patients. Overall, we describe herein the role of an inhibitory receptor involved in the suppression of NK cell activity in CLL, which is restored by ILT2 blockade in combination with lenalidomide, suggesting that it may be an interesting therapeutic strategy to be explored in this disease

A genetic approach reveals different modes of action of prefoldins

[EN] The prefoldin complex (PFDc) was identified in humans as a co-chaperone of the cytosolic chaperonin T-COMPLEX PROTEIN RING COMPLEX (TRiC)/CHAPERONIN CONTAINING TCP-1 (CCT). PFDc is conserved in eukaryotes and is composed of subunits PFD1-6, and PFDc-TRiC/CCT folds actin and tubulins. PFDs also participate in a wide range of cellular processes, both in the cytoplasm and in the nucleus, and their malfunction causes developmental alterations and disease in animals and altered growth and environmental responses in yeast and plants. Genetic analyses in yeast indicate that not all of their functions require the canonical complex. The lack of systematic genetic analyses in plants and animals, however, makes it difficult to discern whether PFDs participate in a process as the canonical complex or in alternative configurations, which is necessary to understand their mode of action. To tackle this question, and on the premise that the canonical complex cannot be formed if one subunit is missing, we generated an Arabidopsis (Arabidopsis thaliana) mutant deficient in the six PFDs and compared various growth and environmental responses with those of the individual mutants. In this way, we demonstrate that the PFDc is required for seed germination, to delay flowering, or to respond to high salt stress or low temperature, whereas at least two PFDs redundantly attenuate the response to osmotic stress. A coexpression analysis of differentially expressed genes in the sextuple mutant identified several transcription factors, including ABA INSENSITIVE 5 (ABI5) and PHYTOCHROME-INTERACTING FACTOR 4, acting downstream of PFDs. Furthermore, the transcriptomic analysis allowed assigning additional roles for PFDs, for instance, in response to higher temperature.This work was supported by grants from the Spanish Ministry of Economy and Competitiveness and "Agencia Estatal de Investigacion"/FEDER/European Union (BIO2013-43184-P to D.A. and M.A.B., and BIO2016-79133-P and PID2019-109925GB-I00 to D.A.). N.B.-T., A.S.-M., and A.P.-A. were recipient of Ministerio de Economia y Competitividad (BES-2014-068868), EU MSCA-IF (H2020-MSCA-IF-2016746396) and Ministerio de Educacion (FPU17/05186) fellowships, respectively.Esteve-Bruna, D.; Blanco-Touriñán, N.; Serrano-Mislata, A.; Esquinas-Ariza, RM.; Resentini, F.; Forment Millet, JJ.; Carrasco-López, C.... (2021). A genetic approach reveals different modes of action of prefoldins. Plant Physiology. 187(3):1534-1550. https://doi.org/10.1093/plphys/kiab348S15341550187