Isolation and Characterization of Plant growth-promoting Endophyticdiazotrophic Bacteria from Sri Lankan Rice Cultivars and Rapid Screening for their effect on Plant Growth Promotion

The present study was conducted to isolate and identify endophyticdiazotrophic bacteria in two Sri Lankan rice (Oryza Sativa L.) varieties; Suwandel and Bg 358 and to evaluate their potential to promote rice plant growth. A total of 15 putative endophyticdiazotrophic bacterial isolates were obtained from shoots and roots of Suwandel and Bg 358 rice varieties out of which 7 isolates were selected based on their ability to produce IAA and phosphate solubilization. According to the morphological characters and biochemical tests, these bacteria were identified belong to genera Bacillus (IN003, IN006, and IN007), Klebsiella (IN008 and IN018), Pantoea (IN009), and Enterobacter (IN015). All selected bacterial isolates produced IAA (7.1µmgl-1 to 30.9 µ mgl-1) in the tryptophan supplemented medium. Five out of seven bacterial isolates (IN006, IN007, IN008, IN015, and IN018) were able to solubilize inorganic phosphate on Pikovskaya\u27s agar medium. Rice seeds (Suwandel variety) treated with these endophyticdiazotrophic bacteria with plant growth-promoting ability showed significantly enhanced shoot length, root length, shoot fresh weight, shoot dry weight and root fresh weight compared to the uninoculated control. Plant inoculation experiment indicated that Enterobacter sp. (IN015) was most effective in rice plant growth promotion among seven bacterial isolates tested. These results strongly suggest that endophyticdiazotrophic bacteria characterized in this study could be successfully used to promote rice plant growth

Coopetition in Sustainable Apparel Manufacturing : An interview study

The apparel industry is one of the biggest environmentally polluting industries globally. In recent years, there has been a shift from the end consumers towards sustainable fashion products due to the concern of the environmental impact of their purchases. Due to this, fashion brands have been trying to offer sustainable products to this growing customer segment. Fashion brands have been requesting apparel manufacturers and the other ecosystem partners involved in the apparel manufacturing process to develop sustainable products. Therefore, the apparel industry has been trying to move into sustainable manufacturing methods due to the pressure from external stake-holders and also due to the growing demand for sustainable products. This has posed several challenges to conventional apparel manufacturers who have developed their business models based on fast fashion. Hence, the objective of this study is to understand the current sustainable goals of the apparel manufacturing industry and also examine the challenges faced by apparel manufacturers when achieving them. Furthermore, whether there are existing coopetition ele-ments in the apparel manufacturing ecosystem. To achieve these research objectives, a case study of a global apparel manufacturer from South Asia was conducted. A series of qualitative interviews was conducted to gather data from the main actors in the organization. The interview data was transcribed and analyzed using the thematic data analysis method to get a broader understanding of the industry. The key findings present the need for a systematic change in the apparel industry in order to make a substantial shift toward sustainability. Particularly in the current 'buyer-driven' business model, where price sensitivity is the central factor in selecting apparel manufacturers, collaborat-ing among vertical actors poses significant challenges, while horizontal actor collaboration re-mains rare. Coopetition is a novel concept in the highly competitive apparel manufacturing indus-try. However, it offers promise as a means to achieve shared sustainable goals among ecosystem partners. Although, it is essential to emphasize that such initiatives need to be spearheaded by fashion brands, given their influential role in the apparel supply chains. In addition, equal commit-ment from the ecosystem partners needs to be ensured in order to mitigate misunderstandings. In conclusion, this study presents the importance of collaboration between ecosystem partners in the apparel manufacturing industry when achieving sustainable goals

Measuring the impact of different brands of computer systems on the clinical consultation: a pilot study

Background UK general practitioners largely conduct computer-mediated consultations. Although historically there were many small general practice (GP) computer suppliers there are now around five widely used electronic patient record (EPR) systems. A new method has been developed for assessing the impact of the computer on doctor_patient interaction through detailed observation of the consultation and computer use. Objective To pilot the latest version of a method to measure the difference in coding and prescribing times on two different brands of general practice EPR system. Method We compared two GP EPR systems by observing use in real life consultations. Three video cameras recorded the consultation and screen capture software recorded computer activity.We piloted semi-automated user action recording (UAR) software to record mouse and keyboard use, to overcome limitations in manual measurement. Six trained raters analysed the videos using data capture software to measure the doctor_patient_computer interactions; we used interclass correlation coefficients (ICC) to measure reliability. Results Raters demonstrated high inter-rater reliability for verbal interactions and prescribing (ICC 0.74 to 0.99), but for measures of computer use they were not reliable. We used UAR to capture computer use and found it more reliable.Coded data entry time varied between the systems: 6.8 compared with 11.5 seconds (P = 0.006). However, the EPR with the shortest coding time had a longer prescribing time: 27.5 compared with 23.7 seconds (P = 0.64). Conclusion This methodological development improves the reliability of our method for measuring the impact of different computer systems on the GP consultation. UAR added more objectivity to the observationof doctor_computer interactions. If larger studies were to reproduce the differences between computer systems demonstrated in this pilot it might be possible to make objective comparisons between systems

Comparison of breast and bowel cancer screening uptake patterns in a common cohort of South Asian women in England

Background: Inequalities in uptake of cancer screening by ethnic minority populations are well documented in a number of international studies. However, most studies to date have explored screening uptake for a single cancer only. This paper compares breast and bowel cancer screening uptake for a cohort of South Asian women invited to undertake both, and similarly investigates these women's breast cancer screening behaviour over a period of fifteen years. Methods: Screening data for rounds 1, 2 and 5 (1989-2004) of the NHS breast cancer screening programme and for round 1 of the NHS bowel screening pilot (2000-2002) were obtained for women aged 50-69 resident in the English bowel screening pilot site, Coventry and Warwickshire, who had been invited to undertake breast and bowel cancer screening in the period 2000-2002. Breast and bowel cancer screening uptake levels were calculated and compared using the chi-squared test. Results: 72,566 women were invited to breast and bowel cancer screening after exclusions. Of these, 3,539 were South Asian and 69,027 non-Asian; 18,730 had been invited to mammography over the previous fifteen years (rounds 1 to 5). South Asian women were significantly less likely to undertake both breast and bowel cancer screening; 29.9% (n = 1,057) compared to 59.4% (n = 40,969) for non-Asians (p < 0.001). Women in both groups who consistently chose to undertake breast cancer screening in rounds 1, 2 and 5 were more likely to complete round 1 bowel cancer screening. However, the likelihood of completion of bowel cancer screening was still significantly lower for South Asians; 49.5% vs. 82.3% for non-Asians, p < 0.001. South Asian women who undertook breast cancer screening in only one round were no more likely to complete bowel cancer screening than those who decided against breast cancer screening in all three rounds. In contrast, similar women in the non-Asian population had an increased likelihood of completing the new bowel cancer screening test. The likelihood of continued uptake of mammography after undertaking screening in round 1 differed between South Asian religio-linguistic groups. Noticeably, women in the Muslim population were less likely to continue to participate in mammography than those in other South Asian groups. Conclusions: Culturally appropriate targeted interventions are required to reduce observed disparities in cancer screening uptakes

A study on the extent, aetiology and associated factors of dandruff in a group of medical students and the in vitro effects of antidandruff preparations

Introduction: Dandruff is a scalp disorder, characterized by itching and rapid skin flaking of the scalp of a person. Yeasts of the genus Malassezia are responsible for dandruff. The prevalence of dandruff in South Asia is 60.1%, whereas in the world it is 50%. The objectives of this study were to ascertain the prevalence, describe associated factors, isolate Malassezia sp. from dandruff samples of medical students and test the anti-fungal effects of commercially available shampoo and crude extracts of commonly used herbs against Malassezia. Methods: Ninety five students were chosen by systematic sampling. A self-administered questionnaire was used for data collection. Samples of skin flakes/swabs was collected from the scalp of participants. Specimens were examined using three direct microscopic methods. Fungal colonies isolated on Saborauds/Dixon Agar were identified using Gram stain and catalase test. Antifungal activity of six shampoo brands and crude extracts of eleven herbs were tested against isolated Malassezia sp. by agar well/disc diffusion method. Results: Prevalence of dandruff was 64.2% in the study group. The typical ‘spaghetti and meatball’ appearance was seen in the skin flakes taken from 44 of 61 (72.1%) students. Malassezia sp. was isolated only from 49 of the 61 students (80.3%). In-vitro, anti-malassezial effect (Inhibition zone (IZ)&gt;10mm) was observed in 4 of the 7 tested ‘anti dandruff’ shampoos. Crude extracts of fresh leaves of Psidium guavava (guava), flowers of Punica grantum (pomegranate) and fruits of Solanum lycopersicum (tomato) and Citrus hystrix (Kaffir lime) showed IZs &gt; 10mm. Conclusion: Prevalence of dandruff in the studied group of medical students was higher than in the Asian population. Four of the tested ‘anti dandruff’ shampoos and crude extracts of leaves of P. guavava (guava), fruits of S. lycopersicum (tomato), C. hystrix (kaffir lime), and flowers of P. grantum (pomegranate) showed an in-vitro inhibitory effect on the growth of Malassezia sp.</p

Prevalence of Polycystic Ovary Syndrome in Women from Opposite-Sex Twin Pairs

Introduction: Intrauterine androgens of a male fetus may influence the female fetus in opposite-sex twin pairs. Because female intrauterine overexposure to androgens could lead to polycystic ovary syndrome (PCOS), the prevalence of PCOS should be higher in women from opposite-sex twin pairs. Therefore, the aim of the current study was to evaluate the prevalence of PCOS in women from opposite-sex twin pairs compared to women from same-sex twin pairs, sisters, and female spouses of twins. Subjects and Methods: Data from 1325 monozygotic twins, 1191 dizygotic twins (711 women from same-sex twin pairs and 480 women from opposite-sex twin pairs), 745 sisters of twins, and 218 spouses of male twins were evaluated. PCOS was defined as less than nine natural menstrual cycles a year combined with either hirsutism or acne. The prevalence of PCOS was compared using a ±2 test. Binary logistic regression analyses were conducted to test for confounding effects of smoking, age, and body mass index. Results: No significant differences in PCOS prevalence were found between women from same-sex twin pairs (either monozygotic or dizygotic), opposite-sex twin pairs, sisters, and spouses. Conclusion: The prevalence of PCOS is not different in women from opposite-sex and same-sex twin pairs, singleton sisters, or spouses. This indicates that possible androgen exposure of the female fetus, caused by a shared intrauterine environment with a male fetus, does not result in PCOS-like traits. Copyright © 2009 by The Endocrine Society

Displays of authority in the clinical consultation: A linguistic ethnographic study of the electronic patient record

AbstractThe introduction of computers into general practice settings has profoundly changed the dynamics of the clinical consultation. Previous research exploring the impact of the computer (in what has been termed the ‘triadic’ consultation) has shown that computer use and communication between doctor and patient are intricately coordinated and inseparable. Swinglehurst et al. have recently been critical of the ongoing tendency within health communication research to focus on ‘the computer’ as a relatively simple ‘black box’, or as a material presence in the consultation. By re-focussing on the electronic patient record (EPR) and conceptualising this as a complex collection of silent but consequential voices, they have opened up new and more nuanced possibilities for analysis. This orientation makes visible a tension between the immediate contingencies of the interaction as it unfolds moment-by-moment and the more standardised, institutional demands which are embedded in the EPR (‘dilemma of attention’). In this paper I extend this work, presenting an in-depth examination of how participants in the consultation manage this tension. I used linguistic ethnographic methods to study 54 video recorded consultations from a dataset collected between 2007 and 2008 in two UK general practices, combining microanalysis of the consultation with ethnographic attention to the wider organisational and institutional context. My analysis draws on the theoretical work of Erving Goffman and Mikhail Bakhtin, incorporating attention to the ‘here and now’ of the interaction as well as an appreciation of the ‘distributed’ nature of the EPR, its role in hosting and circulating new voices, and in mediating participants' talk and social practices. It reveals – in apparently fleeting moments of negotiation and contestation – the extent to which the EPR shapes the dynamic construction, display and circulation of authority in the contemporary consultation

DIABRISK - SL Prevention of cardio-metabolic disease with life style modification in young urban Sri Lankan's - study protocol for a randomized controlled trial

<p>Abstract</p> <p>Background</p> <p>Urban South-Asian's are predisposed to early onset of type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD). There is an urgent need for country specific primary prevention strategies to address the growing burden of cardio-metabolic disease in this population. The aim of this clinical trial is to evaluate whether intensive (3-monthly) lifestyle modification advice is superior to a less-intensive (12 monthly; control group) lifestyle modification advice on a primary composite cardio-metabolic end point in 'at risk' urban subjects aged between 5-40 years.</p> <p>Methods/Design</p> <p>This is an open randomised controlled parallel group clinical trial performed at a single centre in Colombo, Sri-Lanka. A cluster sampling strategy was used to select a large representative sample of subjects aged between 5-40 years at high risk of T2DM and CVD for the intervention study. We have screened 23,298 (males 47% females 53%) healthy subjects for four risk factors: obesity, elevated waist circumference, family history of diabetes and physical inactivity, using a questionnaire and anthropometry. Those with two or more risk-factors were recruited to the intervention trial. We aim to recruit 4600 subjects for the intervention trial. The primary composite cardio-metabolic end point is; new onset T2DM, impaired glucose tolerance, impaired fasting glycaemia, new onset hypertension and albuminuria, following 5 years of intervention. The effect of the intervention on pre-specified secondary endpoints will also be evaluated. The study will be conducted according to good clinical and ethical practice, data analysis and reporting guidelines.</p> <p>Discussion</p> <p>DIABRISK-SL is a large population based trial to evaluate the prevalence of diabetes, pre-diabetes and cardio-metabolic risk factors among young urban Sri-Lankans and the effect of a primary prevention strategy on cardio-metabolic disease end points. This work will enable country specific and regional cardio-metabolic risk scores to be derived. Further if the proposed intervention is successful the results of this study can be translated and implemented as a low-cost primary prevention tool in Sri-Lanka and other low/middle income developing countries.</p> <p>Trial registration</p> <p>The trial is registered with the World Health Organisation and Sri-Lanka clinical trial registry number SLCTR/2008/003</p

Overexpression of Nrdp1 in the Heart Exacerbates Doxorubicin-Induced Cardiac Dysfunction in Mice

BACKGROUND: Cardiac cell death and generation of oxidative stress contribute to doxorubicin (DOX)-induced cardiac dysfunction. E3 ligase Nrdp1 plays a critical role in the regulation of cell apoptosis, inflammation and production of reactive oxygen species (ROS), which may contribute to heart failure. However, the role of Nrdp1 in DOX-induced cardiac injury remains to be determined. METHODS AND RESULTS: We examined the effect of Nrdp1 overexpression with DOX treatment in rat neonatal cardiomyocytes and mouse heart tissue. Cardiomyocytes were infected with adenovirus containing GFP (Ad-GFP), Nrdp1 wild-type (Ad-Nrdp1) or the dominant-negative form of Nrdp1 (Ad-Dn-Nrdp1), then treated with DOX for 24 hr. DOX treatment increased cell death and apoptosis, with Ad-Nrdp1 infection enhancing these actions but Ad-Dn-Nrdp1 infection attenuating these effects. Furthermore, 5 days after a single injection of DOX (20 mg/kg, intraperitoneally), Nrdp1 transgenic mice (TG) showed decreased cardiac function and increased apoptosis, autophagy and oxidative stress as compared with wild-type (WT) mice (P<0.01). Survival rate was significantly lower in Nrdp1 TG mice than in WT mice 10 days after DOX injection (P<0.01). CONCLUSIONS/SIGNIFICANCE: These results were associated with decreased activation of Akt, extracellular signal-regulated kinase 1/2 (ERK1/2) and signal transducer and activator of transcription 3 (STAT3) signaling pathways. Nrdp1 may be a key mediator in the development of cardiac dysfunction after DOX treatment and associated with inhibition of Akt, ERK1/2 and STAT3. Nrdp1 may be a new therapeutic target in protecting against the cardiotoxic effects of DOX

UNderstanding uptake of Immunisations in TravellIng aNd Gypsy communities (UNITING): Protocol for an exploratory, qualitative study

© BMJ Open 2015. Introduction: Gypsies, Travellers and Roma (referred to here as Travellers) experience significantly poorer health and have shorter life expectancy than the general population. They are also less likely to access health services including immunisation. To improve immunisation rates, we need to understand what helps and hinders individuals in these communities in taking up immunisations. This study has two aims: (1) Investigate the barriers and facilitators to acceptability and uptake of immunisations among six Traveller communities in the UK; (2) Identify potential interventions to increase uptake in these Traveller communities. Methods and analysis: A three-phase qualitative study with six Traveller communities. PHASE 1: In each community, we will explore up to 45 Travellers' views about the influences on their immunisation behaviours and ideas for improving uptake in their community. PHASE 2: In each community, we will investigate 6-8 service providers' perspectives on barriers and facilitators to childhood and adult immunisations for Traveller communities with whom they work, and ideas to improve uptake. Interview data will be analysed using the Framework approach. PHASE 3: The findings will be discussed and interventions prioritised in six workshops, each with 10-12 phase 1 and 3-4 phase 2 participants. Ethics and dissemination: This research received approval from NRES Committee Yorkshire and The Humber-Leeds East (Ref. 13/YH/02). It will produce (1) findings on the barriers and facilitators to uptake of immunisations in six Traveller communities; (2) a prioritised list of potentially feasible and acceptable interventions for increasing uptake in these communities; and (3) methodological development in undertaking research with diverse Traveller communities. The study has the potential to inform new ways of delivering services to ensure high immunisation uptake. Findings will be disseminated to participants, relevant UK organisations with responsibility for the implementation of immunisation policy and Traveller health/welfare; and submitted for publication in academic journals